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Leica DMS300 Swing-arm StandBaseplate and Vertical ColumnInstructions for safe assemblyDue to the heavy weight of the baseplate, improper use can cause injuriesor damage to the surrounding area. For safetyreasons, use two people for this part of thesetup.Assembling the column1. Push the tapping screw through the baseplate from below.2. Fit the serrated lock washer onto the screw.3. Install the vertical column on the base plateusing the screw.3213Leica DMS300 User ManualAssembly 18

Safety Ring and Horizontal ArmInstalling the safety ringMounting the horizontal armFastening the horizontal arm122112131. Push the safety ring over the column.2. Securely tighten the clamping screw.The safety ring is the most importantsafety element of the entire configuration. It protects the horizontal arm from accidentally falling down.Leica DMS300 User Manual1. Push the cross-member with the horizontal arm over the column so that the crossmember lies securely on the safety ring.2. You can now rotate the horizontal arm inany direction desired.The swing-arm stand is designed sothat the Leica DMS300 can be moved asdesired.1. Rotate the horizontal arm into a secure position.2. Carefully tighten the clamping screw on thecross-member to prevent the joint on thecolumn from rotating.3. Tighten the clamping screw to prevent thearm from extending.For safety reasons, you should fasten thehorizontal arm in place when you leaveyour workstation.Assembly 19

10" MonitorInstall on the horizontal armInstall on the vertical arm2214131. Remove the clamping screw.2. Install the monitor holder on the horizontalarm.3. Fasten the monitor holder to the clampingscrew.1. Install the monitor holder on the verticalarm.2. Fasten the monitor holder to the clampingscrew.4. Reinstall the clamping screw.Leica DMS300 User ManualAssembly 20

Microscope Carrier and Leica DMS300Assembling the microscope carrierFree movement of the microscopeAssembling the Leica DMS300LEICA DMS30022211. Insert the connecting pin from below intothe bore of the horizontal arm.2. Fasten the microscope carrier in place usingthe washer and safety screw.131. Unscrew the clamping screw.2. Place the Leica DMS300 in the microscopecarrier so that the notch in the microscopehousing and the lower clamping screwoverlap.The microscope can be rotated freely togetherwith the microscope carrier.3. Fasten the microscope in place using theclamping screw.Leica DMS300 User ManualAssembly 21

Objective InstallationInstructions for safe assemblyHold the objective firmly during assembly and disassembly so that it does notfall onto the stage plate. Remove all specimensfrom the stage plate first.Assembly 11. Remove the protective cap on the opticscarrier by turning it.Leica DMS300 User Manual22. Screw the objective into the optics carrier.Assembly 22

Installing the Leica Ring LightAssembly of the Leica ring light illuminator3001231. Connect the external power supply unit tothe Leica ring light illuminator.2. Attach the Leica ring light illuminator to theobjective from below.Ensure that the locking screw of the illuminator and the locking screw of theoptics carrier are on a straight line.Connect the power supply to the power cable.3. Tighten the locking screw.Leica DMS300 User ManualAssembly 23

Illumination: Leica Ring Light Illuminator (Continued)Assembly of the diffuser11. The diffuser is attached and screwed inbelow the ring light illuminator.We recommend assembling the diffuseron the Leica ring light illuminator on atable in reverse and then mounting them on theobjective together.Leica DMS300 User ManualAssembly 24

Leica DMS300 With Incident Light StandIncident Light Base and Focusing ColumnGeneral notesThis chapter shows an example of assembly of the Leica DMS300 on an incidentlight base. Images and descriptions may varywhen using another incident light base.Assembling the column1. Take the three provided screws out of thepackaging.2. Install the incident light base on the columnusing the three screws.12Leica DMS300 User ManualAssembly 25

Microscope Carrier and Leica DMS300Assembling the Leica DMS300Assembling the microscope carrier2LEICA DMS300211. Fasten the microscope carrier to the columnusing the provided Allen key and theprovided screw (recommended variant forobjectives with a small working distance).122. Alternatively, the microscope carrier canalso be installed upside-down (recommended variant for objectives with a largeworking distance).2131. Unscrew the clamping screw.2. Place the Leica DMS300 in the microscopecarrier so that the notch in the microscopehousing and the lower clamping screwoverlap.3. Fasten the microscope in place using theclamping screw.Leica DMS300 User ManualAssembly 26

10" MonitorInstall on the column1. Align the mounting pins so that they arealigned with the vertical slot in the back ofthe focus column.2. Insert the pins into the slot so that themounting bracket is firmly seated on thefocus column3. Rotate the thumb screw so that the mounting pins rotate 90 clockwise.Leica DMS300 User Manual4. Slightly pull out on the thumb screw so thatthe mounting pins remain in place.5. While pulling out on the thumb screw,rotate clockwise to lock the monitor mounting bracket in place.7. Once the monitor bracket is secure, adjustthe monitor using the knob on the mounting arm to move the monitor to the optimalworking position.6. The bracket can be positioned as desired onthe back of the column.Assembly 27

Illumination: Installing the Leica LED3000 RLInstalling the Leica LED3000 RL1231. Connect the external power supply unit(10 450 266) to the Leica LED3000 RL.2. Attach the Leica LED3000 RL to the objective from below.4Ensure that the locking screw of the illuminator and the locking screw of theoptics carrier are on a straight line.Connect the power supply to the power cable.3. Tighten the locking screw.The supported working distance isbetween 60 mm and 150 mm.Leica DMS300 User ManualAssembly 28

Illumination: Installing the Leica LED3000 RL (Continued)Installing optional accessories11. The optional diffuser is attached andscrewed in below the ring illuminator.We recommend assembling the accessories on the Leica LED3000 RL on a table inreverse and then mounting them on the objective together.Leica DMS300 User Manual11. The optional polarization set (polarizer andanalyzer) is attached and screwed in belowthe ring illuminator. The provided smallmetal plate is used as a fastening aid in theprocess.Assembly 29

InstallationLeica DMS300 User ManualInstallation 30

HD Mode (Standalone)Cables and Terminals, Activating HD Mode (Standalone)General instructionsThe Leica DMS300 is designed for the useof HD-capable (high definition)monitors. We highly recommend connecting the HDmonitor using an HDMI connector and not usinga DVI adapter, as otherwise proper function canno longer be guaranteed!Please only connect the provided 5 Vpower supply unit. Using an incorrect voltage can substantially damage theLeica DMS300.Activating HD mode (standalone)Power supply and monitor2bPCHDFOF12a1. Set the mode switch to "HD" in order to setthe Leica DMS300 to HD mode (standalone).2I1. Connect the Leica DMS300 to a suitableDMHpower socket using the USB cable andpower supply.2a. Connect the Leica DMS300 to the HD monitor using the HDMI cable. Or:2b. Connect the Leica DMS300 to the 10" monitor using the HDMI cable. Connect the10" monitor to a suitable power socket.Leica DMS300 User ManualONAn SD card with sufficient free memorymust be inserted into the Leica DMS300in order to save images in HD mode (standalone).In order to avoid instrument malfunctions, do not connect the Leica DMS300to a computer while in HD mode (standalone).Installation 31

PC ModeConnecting a Computer, Installing Software, Activating PC ModeInstallation and connectionFor instructions on installing and usingthe software for PC mode, refer to theDVD provided with the Leica DMS300.1. Place the DVD provided into the computer'sdisk drive and follow the instructions forinstalling the software.Activating PC modeThe Leica DMS300 is powered by thecomputer via the USB cable. This changesthe color of the status light. The status light isred when switching on the Leica DMS300; assoon as it is green, you can open the softwareand view, record or manage images.PC222. Connect the Leica DMS300 to the computerusing the USB cable.Leica DMS300 User ManualONHDFOF1. Set the mode switch to "PC" in order to setthe Leica DMS300 to PC mode.ONHDPCFOFMIHD3. Follow the instructions in the software foradjusting and recording an image.Leica Application Suite has to be installedon the computer in order to use theLeica DMS300 in PC mode. The computer alsohas to have sufficient free hard drive space.Installation 32

Limitations in PC ModeOperationThe Leica DMS300 is controlled entirelyvia the computer in PC mode. The frontbutton, the remote control and the footswitchdo not work. The menus for the camera are alsonot displayed on the HD monitor. Status information can be retrieved at any time despite thisby usingon the remote control.Aspect ratioImages cannot be saved onto the SD cardin PC mode.4:316:9Images are always output in a 4:3aspect ratio when the Leica DMS300 isconnected to a computer – even if a 16:9 monitor is connected via HDMI. However, the imageis displayed correctly on these kinds of devices,just with black on the screen edges.Leica DMS300 User ManualInstallation 33

Startup ProcedureLeica DMS300 User ManualStartup Procedure 34

Leica DMS300Switching on the Leica DMS300HD mode (standalone)PCONHDPC ModeIf the Leica DMS300 is connected to anHD monitor in PC mode:1OFOF2Ensure that the objective is screwed ontothe microscope, the mode switch is set to"HD" and the HD monitor is switched on.PCthe remote control does not respond;in other words, the user menu does notappear.OOn the HD monitor, a live image is shownin 4:3 aspect ratio (with black bars right andleft).OThe menu does not appear.OThe message "No camera" appears whenstarting the LAS-EZ software.ONHD1FOF3Ensure that the objective is screwed ontothe microscope, the mode switch is set to"PC" and the computer monitor is switched on.1. Set the on/off switch to "ON" to switch onthe Leica DMS300.1. Set the on/off switch to "ON" to switch onthe Leica DMS300.2. The status light's LED switches from red togreen, a signal tone sounds and the liveimage is displayed on the HD monitor - theLeica DMS300 is ready to use.2. Start Leica Application Suite.Leica DMS300 User Manual3The status light's LED switches from red togreen, a signal tone sounds and the liveimage is displayed on the computer – theLeica DMS300 is ready to use.Startup Procedure 35

Switching on the IlluminationGeneral instructionsThe light from the Leica LED illuminatorcan be very bright. Avoid looking directlyinto the LEDs.The intensity of the illuminator can beadjusted in 10 increments.When pressing the buttons, hold thekeypad between your thumb and indexfinger. Avoid tapping the keypad with just onefinger if possible.Leica DMS300 User ManualUse11. Switch on the illuminator by briefly pressingthe(ON/OFF) button. The green LED onthe upper left corner now lights up.232. Adjust the brightness by briefly pressing theorbuttons.3. Switch off the illuminator by briefly pressingthebutton.Startup Procedure 36

Focusing, Adjusting the Resistance of the Focus DriveFocusingFocusing raises or lowers the microscope usingthe focusing drive. The specimen detail isbrought into sharp focus as soon as it is in thefocal point of the objective.OThe focusing drive can be operated eitherleft- or right-handed.Adjusting the resistanceIs the focusing drive too loose or too tight? Doesthe equipment tend to slide downwards? Theresistance can be adjusted individually depending on the equipment weight and personal preferences as follows:300300111. Grip the drive knobs with both hands andturn them towards each other until thedesired resistance is reached during focusing.Boom StandLeica DMS300 User ManualIncident light baseStartup Procedure 37

Changing Magnification, Click-stop FeatureEnabling and disabling click stopsChanging the magnification (zoom)2321. Look at the HD monitor.2. Focus on the specimen.3. Rotate the magnification changer until thedesired magnification is configured or it hasto be refocused.5.04.053.31300The magnification changer can optionallybe operated either with or without clickstops enabled. Continuous zoom is possiblewhen the click stops are disabled, which manyusers find convenient.6.01Parfocality: The Leica DMS300 is parfocally matched. If the specimen is in focusat the highest magnification level, this remainstrue across the entire magnification range.21. Push the button towards the knob to enablethe click stops.2. Push the button away from the knob todisable the click stops.The rotary knob for the zoom can be usedeither left or right-handed.Leica DMS300 User ManualStartup Procedure 38

Remote ControlGeneral instructionsDescription101112Always keep the remote control for controllingthe Leica DMS300 pointed towards the microscope's IR receiver.The remote control does not respondif the microscope is connected to acomputer.Instead of the remote control, the frontbutton or the footswitch (depending onthe configuration) can be used to trigger theLeica DMS300.Leica DMS300 User Manual13132564781. Start/stop video recording2. Save still image to SD card3. Stop/continue live image,pause/play movie clip4. View files on SD card5. Move to previous image on SD card6. Move to next image on SD card7. Brightness adjustment up or down8. Pairing button9. Show/hide information box10. White balance set button11. Call up the camera menu12. Arrow keys for navigation13. OK to confirm79Startup Procedure 39

Remote Control (Continued)Changing the batteriesChecking the batteries1. Switch on the microscope and HD monitorso that you see the live image on the HDmonitor.122Leica DMS300 User Manual2. Hold the remote control in the beam pathso that it faces upwards and press any key.1. Remove the battery cover on the rear side ofthe remote control.Because the microscope's chip also responds tothe infrared range, you can see how the LED ontop of the remote control lights up on the HDmonitor. If this is not the case, the batteries needto be changed.2. Replace the batteries (2x AAA) and close thebattery cover.For the battery replacement, you needtwo (2) type AAA batteries.Startup Procedure 40

Pairing Cameras With RemotesPairingThe Leica DMS300 and the remotecontrol can be paired and then onlyrespond to each other. This can be helpful whenusing multiple cameras and remote controls.1. Press theprocess.button to start or end the3. The microscope only responds to theremote control commands from the remotecontrol used for the pairing.Resetting to factory setting1. Press the buttonto start the process.until a corresponding2. Press the buttonconfirmation is displayed on the HD monitor.In order to achieve successful pairingand avoid pairing by mistake, the secondstep must be performed within 4 seconds.If a "timeout" is displayed after 4 seconds,press the "Pair" button again to start theprocess.2. Press and hold the button on your remotecontrol representing the channel that youwant to assign to the connection (seeimage to the right, channel selection) untila corresponding confirmation is displayedon the HD monitor. All of the buttonsexcept thebutton can be used for this.Leica DMS300 User ManualSee more on this topic in our online videos atour website www.leica-microsystems.com.Startup Procedure 41

Configuring the Front Button or FootswitchGeneral instructionsConfiguring1. Point the remote control towards theLeica DMS300.4. Select "FRONT BUTTON" for the front buttonor "FOOT SWITCH" for the footswitch:2. Press the buttonto show the user menuon the computer monitor.3. Select "SETUP USER" in the main menu:Both the Leica DMS300's front button andfootswitch can be configured with the remotecontrol as defined by the user.The default setting is "CAPTURE" forcapturing an image.The Leica DMS300's front button andfootswitch do not work in PC mode.Leica DMS300 User ManualStartup Procedure 42

Configuring the Front Button or Footswitch (Continued)5. The following configurations are possible byselecting the corresponding option:ONONE: No FunctionOCAPTURE: Capture an image and save it tothe SD card.OSHOW LAST: Show a preview of the mostrecently acquired image.OMOVIE: Start video recording or stop a videorecording that is in progress. Saved to theSD card as an MP4 file.OOVERLAY: Show crosshair or overlay. Pressing this button multiple times scrollsthrough the list of crosshairs or overlays.OWHITE BAL: Run white balance for colorcorrecting the camera. For additional information on white balance, refer to page 50.See more on this topic in our online videos atour website www.leica-microsystems.com.Leica DMS300 User ManualStartup Procedure 43

OperationLeica DMS300 User ManualOperation 44

HD Mode (Standalone)Checking Cables and TerminalsGeneral instructionsThe Leica DMS300 is designed for the useof HD-capable (high definition) monitors.We highly recommend connecting the HD monitor using an HDMI connector and not using aDVI adapter, as otherwise proper function can nolonger be guaranteed!Please only connect the provided 5 Vpower supply unit. Using an incorrect voltage can substantially damage the Leica DMS300.HD mode (standalone)Power supply and HD monitor2bPCONHDFOF12a1. Check whether the mode switch is set to"HD" (standalone).2IM1. Check that the Leica DMS300 is properlyHDsupplied with power via the USB powersupply.An SD card with sufficient free memorymust be inserted into the Leica DMS300in order to be able to save images in HD mode(standalone).2a. Check whether the Leica DMS300 isconnected to the HD monitor properly via theHDMI cable. Or:2a. Check whether the Leica DMS300 isconnected to the 10" monitor with the HDMIcable and the monitor is attached to a powersocket.Leica DMS300 User ManualOperation 45

Switching on the Leica DMS300Switching on the Leica DMS300 (standalone)En

Leica DMS300 User Manual General Instructions 9 Safety Instructions (continued) Repairs, service work O ORefer to "Safety Concept" booklet O Only original Leica Microsystems spare parts may be used. O Before opening the instruments, switch off the power and unplug the power cable. O Avoid con