Transcription
Analytical Methods Approved for Compliance Monitoring under theGround Water RuleAnalysis for the following contaminants shall be conducted in accordance with the methods in the following table, or their equivalent asdetermined by EPA. The methods and monitoring requirements for these contaminants are specified in 40 CFR 141.402(c)(2).Additional methods are listed in Appendix A to Subpart C of Part 141.The CFR is the legal reference for approved methods and takes precedent over this table. The table should accurately reflect theanalytical methods information published in 40 CFR 141.§141.402(c)(2)– Analytical Methods(1) The time from sample collection to initiation of analysis may not exceed 30 hours. The ground water system is encouraged but is not required to holdsamples below 10 C during transit.(2) Collection of a standard sample volume of at least 100 mL for fecal indicator analysis regardless of the fecal indicator or analytical method used(§141.402(b)(3))(3) Analysis of all ground water source samples using one of the analytical methods listed in the §141.402(c)(2) of this section for the presence of E.coli,enterococci, or coliphage.The procedures must be conducted with the documents listed at §141.402(c)(2) or one of the alternative methods listed in Appendix A to Subpart C of Part 141.For Standard Methods Online, the year in which each method was approved by the Standard Methods Committee is designated by the last two digits followingthe hyphen in the method number. The methods listed are the only online versions of the method that maybe used. For vendor methods, the date of themethod listed in §141.402(c)(2) or Appendix A to Subpart C of Part 141 is the date/version of the approved method. Laboratories should be careful to use onlythe approved versions of the methods as product packaging inserts may not be the same as the approved versions of the methods.(a) Laboratory certification- systems must have all compliance samples required under this subpart analyzed by a laboratory certified by EPA or a primacystate to analyze drinking water samples. The laboratory used by the system must be certified for each method (and associated contaminant(s)) used forcompliance monitoring analyses under this rule.(b) Incorporate by reference – The methods required in §141.402(c)(2) are incorporated by reference into the regulation.ContaminantEscherichia coli - Procedure (following Lactose Fermentation Methods):Office of Water (MS – 4606 M)EPA 815-B-19-006April 2019
Page 2 of 8MethodOrganization9221 FStandard Methods9221 FStandard Methods9221 FStandard Methods9221 F -06Standard Methods OnlineReference Title/SourceStandard Methods for theExamination of Water andWastewater, 20th EditionStandard MethodsStandard Methods for theExamination of Water andWastewater, 22nd EditionStandard MethodsStandard Methods for theExamination of Water andWastewater, 23rd EditionStandard MethodsOnline version. Approval year isdesignated by the last 2 digits.Only online versions cited in theregulations or in Appendix A toSubpart C of Part 141 areapproved.Standard MethodsDateNotes1998EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can beused for E.coli testing step after use of Standard Methods9221B, 9221D, 9222B, or 9222C2012EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can beused for E.coli testing step after use of Standard Methods9221B, 9221D, 9222B, or 9222C2017EC-MUG (Method 9221F) can be used for E.coli testing stepafter use of Standard Methods 9221B, 9221D, 9222B, or 9222C2006EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can beused for E.coli testing step after use of Standard Methods9221B, 9221D, 9222B, or 9222CDateNotes1998EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can beused for E.coli testing step after use of Standard Methods9221B, 9221D, 9222B, or 9222C2017NA-MUG (Method 9222I) can be used for E.coli testing stepafter use of Standard Methods 9221B, 9221D, 9222B, or 9222CContaminantEscherichia coli - Partition methods:MethodOrganization9222 G.1c(2)Standard Methods9222 IStandard MethodsContaminantEscherichia coli - membrane filtration methods:Reference Title/SourceStandard Methods for theExamination of Water andWastewater, 20th EditionStandard MethodsStandard Methods for theExamination of Water andWastewater, 23rd EditionStandard Methods
Page 3 of 8MethodOrganization1604EPAmColiBlue24 TestHach Company9222 JStandard MethodsChromocultEMD MilliporeReference Title/SourceMethod 1604: Total Coliformsand Escherichia coli in Water byMembrane Filtration Using aSimultaneous DetectionTechnique (MI Medium),September 2002EPA Method 1604Membrane Filtration Method mColiBlue24 Broth, Revision 2,August 17, 1999m-ColiBlue 24 TestStandard Methods for theExamination of Water andWastewater, 23rd EditionStandard MethodsChromocult Coliform AgarPresence/Absence MembraneFilter Test Method for Detectionand Identification of ColiformBacteria and Escherichia coli forFinished Waters, November2000, Version scherichia coli - enzyme substrate methods:MethodOrganization9223 BColilert Standard Methods9223 BColilert Standard Methods9223 BColilert Standard MethodsReference Title/SourceStandard Methods for theExamination of Water andWastewater, 20th EditionStandard MethodsStandard Methods for theExamination of Water andWastewater, 21st EditionStandard MethodsStandard Methods for theExamination of Water andWastewater, 22nd EditionStandard MethodsDate199820052012Notes
Page 4 of 8MethodOrganization9223 BColilert Standard Methods9223 B-97Colilert Standard Methods Online9223 B-04Colilert Standard Methods Online9223 BColisure Standard Methods9223 BColisure Standard Methods9223 BColisure Standard Methods9223 BColisure Standard Methods9223 B-97Colisure Standard Methods OnlineReference Title/SourceStandard Methods for theExamination of Water andWastewater, 23rd EditionStandard MethodsOnline version. Approval year isdesignated by the last 2 digits.Only online versions cited in theregulations or in Appendix A toSubpart C of Part 141 areapproved.Online version. Approval year isdesignated by the last 2 digits.Only online versions cited in theregulations or in Appendix A toSubpart C of Part 141 areapproved.Standard Methods for theExamination of Water andWastewater, 20th EditionStandard MethodsStandard Methods for theExamination of Water andWastewater, 21st EditionStandard MethodsStandard Methods for theExamination of Water andWastewater, 22nd EditionStandard MethodsStandard Methods for theExamination of Water andWastewater, 23rd EditionStandard MethodsOnline version. Approval year isdesignated by the last 2 digits.Only online versions cited in theregulations or in Appendix A toSubpart C of Part 141 tes
Page 5 of 8MethodOrganization9223 B-04Colisure Standard Methods Online9223 BColilert-18 Standard Methods9223 BColilert-18 Standard Methods9223 BColilert-18 Standard Methods9223 BColilert-18 Standard Methods9223 B-97Colilert-18 Standard Methods Online9223 B-04Colilert-18 Standard Methods OnlineReference Title/SourceOnline version. Approval year isdesignated by the last 2 digits.Only online versions cited in theregulations or in Appendix A toSubpart C of Part 141 areapproved.Standard Methods for theExamination of Water andWastewater, 20th EditionStandard MethodsStandard Methods for theExamination of Water andWastewater, 21st EditionStandard MethodsStandard Methods for theExamination of Water andWastewater, 22nd EditionStandard MethodsStandard Methods for theExamination of Water andWastewater, 23rd EditionStandard MethodsOnline version. Approval year isdesignated by the last 2 digits.Only online versions cited in theregulations or in Appendix A toSubpart C of Part 141 areapproved.Online version. Approval year isdesignated by the last 2 digits.Only online versions cited in theregulations or in Appendix A toSubpart C of Part 141 areapproved.Date2004199820052012201719972004Notes
Page 6 of 8MethodOrganizationE*Colite Charm SciencesReadycult EMD MilliporeModifiedColitag CPI International, Inc.Tecta EC/TC, v.1.0Veolia Water Solutions andTechnologiesTecta EC/TC, v.2.0Pathogen Detection Systems, Inc.ContaminantReference Title/SourceCharm E*Colite Presence/Absence Test forDetection and Identification ofColiform Bacteria andEscherichia coli in DrinkingWater,January 9, 1998Charm E*ColiteReadycult Coliforms 100Presence/Absence Test forDetection and Identification ofColiform Bacteria andEscherichia coli in FinishedWaters,January 2007,Version 1.1ReadycultModified Colitag Test Methodfor the Simultaneous Detectionof E. coli and other TotalColiforms in water (ATP D050035), August 28, 2009Modified ColitagTecta EC/TC medium and theTecta Instrument: APresence/Absence Method forthe Simultaneous Detection ofTotal Coliforms and Escherichiacoli (E. coli) in Drinking Water,May 2014, Version 1.0Tecta, Version 1.0Tecta EC/TC medium and theTecta Instrument: APresence/Absence Method forthe Simultaneous Detection ofTotal Coliforms and Escherichiacoli (E. coli) in Drinking Water,March 2017, Version 2.0Tecta, Version 2.0Date1998200720092014Notes
Page 7 of 8Enterococci – Multiple Tube Technique methods:MethodOrganization9230 BStandard Methods9230 B-04Standard Methods OnlineEnterococci – membrane filtration methods:MethodOrganization9230 CStandard Methods9230 CStandard Methods1600EPAEnterococci – enzyme substrate methods:Reference Title/SourceStandard Methods for theExamination of Water andWastewater, 20th EditionStandard MethodsOnline version. Approval year isdesignated by the last 2 digits.Only online versions cited in theregulations or in Appendix A toSubpart C of Part 141 areapproved.Standard MethodsReference Title/SourceStandard Methods for theExamination of Water andWastewater, 20th EditionStandard MethodsStandard Methods for theExamination of Water andWastewater, 23rd EditionStandard MethodsEPA Method 1600: Enterococciin Water by MembraneFiltration Using membraneEnterococcus Indoxyl-β-DGlucoside Agar (mEI)EPA 821-R-02-022September 2002EPA Method 1600 (2002)DateNotes19982004Date199820172002Notes
Page 8 of 8MethodOrganizationEnterolertApplied and EnvironmentalMicrobiology9230 DStandard MethodsReference Title/SourceBudnick, G.E., R.T. Howard, D.R.Mayo. 1996. “Evaluation ofEnterolert for Enumeration ofEnterococci in RecreationWaters”. Applied andEnvironmental Microbiology.62:3881-3884.Standard Methods for theExamination of Water andWastewater, 23rd EditionStandard MethodsDateNotes19962017Fluorogenic Substrate Enterococcus Test using nization1601EPA1602EPAFast Phage Charm SciencesReference Title/SourceEPA 1601: Male-specific (F ) andSomatic Coliphage in Water byTwo-step EnrichmentProcedure,April 2001EPA Method 1601 (2001)EPA 1602: Male-specific (F ) andSomatic Coliphage in Water bySingle Agar Layer (SAL)Procedure,April 2001EPA Method 1602 (2001)Fast Phage TestPresence/Absence for Coliphagein Ground Water with Same DayPositive Prediction, ATP CaseNo. D09-0007, Version 009,November 28, 2012Fast Phage Test, v. 009200120012012
Standard Methods for the Examination of Water and Wastewater, 20. th. Edition Standard Methods 1998 EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can be used for . E.coli. testing step after use of Standard Methods 9221B, 9221D, 9222B, or 9222C 9221 F Standard Methods . Standard Methods for the Examination of Water and Wastewater, 22. nd. Edition
