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CyFlow Cube 6 ImmunologyMicrobiologyIndustrial ApplicationsAgrosciences AquacultureFlow Cytometry: Simply elegant. Elegantly simple.
01 esforHealthcareforMibio crologyMedical Diagnostics ResearchMicrobiology IndustryImmunologyDNA AnalysisCell Cycle AnalysisCell CountingQuality Control in DairyHIV/AIDSStem CellsCell ProliferationViabilityLeukemiaApoptosisIndustry & Milk ProductsFermentation Process ControlLymphomaCell CulturesCytokinesApplications Live/Dead AnalysisCell Cycle AnalysisPlatelet CountingHematologyAbsolute CellLeukocyte DepletionPathologyCancer Research2 ApplicationsCountingViabilityLive/Dead AnalysisQuality Control inFood & Beverage IndustryToxicologyDetection of Marine Biology & Algae
The CyFlow Cube 6 has been designed by Partec as a high performance systemwhich offers you the best and most reliable tool for routine and research work.Furthermore, the advanced flow cytometry technology of the CyFlow Cube 6 iscapable of a wide range of applications.The Perfect Solution for all yourCell Analysis Applications: CyFlow Cube 6Agrosciences Breeding AquacultureBioreactor ProcessOptimisationDetection ofPloidy LevelParticle CountingPlant Genome SizePharmaceutical IndustryDNA AnalysisQuality ControlAneuploidsin Cosmeticsand AllopolyploidsDetection ofApomixis andReproductionBehaviourDetection of HybridsPolysomyPolysomaty and PlantSperm Cell ViabilityChimera AnalysisGender DeterminationSperm Cell FunctionCell Type Identificationin Natural PopulationsSperm Cell CountingResearchApplications 3
15"Unique Instrument DesignBuilt-in 15” TFT ScreenTwo different Wavelengths available02 HighlightsMade in Germany4 Highlights4
Intuitive Powerful FCM SoftwareOptional CyFlow Robby 6 Autoloading StationMinimal Setup TimeUNIQUE FLOW CYTOMETRY DESIGN BY PARTEC.Superior Performance. Most Cost-effective Solution.The CyFlow Cube 6 from Partec opens a new dimension in flow cytometry.CyFlow Cube 6Optical Parameters (Colors)High-performance, bench-top designwith fully-integrated fluidics, built-inPC and a 15" TFT monitor6 (4 Colors FSC SSC)488 nm and 638 nm laser modulesLight Sources1 or 2 lasers (488 nm blue and 638 nm red)(other wavelengths available upon request)Options & UpgradesCyFlow Robby 6 Autoloading Stationfor tubes and well platessuperior fluorescence sensitivity: 100 MESF (FITC) 50 MESF (PE)down to nanotechnology: superiorsmall particle detection 50 nmflexible and modular CyFlow Cube 6system configurations optional CyFlow Robby 6 AutoloadingStation for well plates and tubesCyFlow Cube 6 — Selection of Available Light SourcesExcitation (nm)DetectorBlue 488GreenFITCGFPAlexa Fluor 488OrangePEYFPHydroethidineRed 638Exemplary DyesSyto 9-24Oregon GreenOrange RedPE-Texas RedPIECDEBRed IPE-Cy5PerCPPE-Dy647Acridine Orange7-AADRed IIPE-Cy5.5PerCP-Cy5.5Far RedPE-Cy7Syto 59-63Dy647TO-PRO3Alexa Fluor 750Cy7Red IAPCAPC-Cy5Red IIAPC-Cy5.5Cy5.5Far RedAPC-Cy7APC-H7JC-1 (monomers) DiOC6(3)H2-DCF-DAJC-1 (aggregates)Alexa Fluor 647Draq5Cy5Highlights 5
Extremely Small DimensionsAdditional 2nd Screen SupportAutoloader for Tubes and Well Plates03 Instrument DesignFULL FLEXIBILITY FOR YOUR APPLICATIONS.The CyFlow Cube 6 offers flow cytometrists the most cost-efficient wayto be perfectly equipped for current and new applications.Intuitive easy-to-use flexibleflow cytometer for any laboratoryTruly stand-alone and fully integratedhigh performance instrumentThe CyFlow Cube 6 impressively demonstrates how state-With its small dimensions of only L 381 W 280 H 279,5 mm,of-the-art flow cytometry technology reduces set-up time andthe 2-laser CyFlow Cube 6 features built-in Windows PC,maintenance to a minimum, achieves intuitive easy instrument15" TFT screen (additional 2nd screen support), software-operation and therefore offers the highest possible time andcontrolled pressure regulators and near-by sheath / wastecost efficiency in your daily laboratory work.containers. Additional space on or under your laboratorybench is no longer required. The CyFlow Cube 6 is equippedDue to its unique design and superior performance charac-with standard interfaces for USB, LAN, video output, etc.teristics, the CyFlow Cube 6 covers a wide range of appli cations, both in the medical and the non-medical fields.The high performance and computing power of the CyFlow The CyFlow Cube 6 has been built for offering a perfectCube 6 allows real-time signal analysis, real-time signalworkflow in your daily routine use. Furthermore, due to itsprocessing and real-time display of each event generated bymost modern system architecture, based on the latesta cell or particle. This unique capability of an entirely real-timegeneration of cutting-edge component technologies (e.g.performing flow cytometer is a prerequisite for precise highstate-of-the-art laser modules, optoelectronics, signalspeed analysis and accurate absolute counting. More thanprocessing boards, etc.), the CyFlow Cube 6 is surprisinglythis, the CyFlow Cube 6 offers the optimum in fluorescencecompact and robust. Sample autoloading is optionallysensitivity, DNA quantification, scatter resolution and smallavailable with the CyFlow Robby 6 Autoloading Stationparticle detection down to nanotechnology size (e.g. for virusfor well plates and sample tubes. Laboratories equippedor bacteria analysis). with the CyFlow Cube 6 therefore benefit from the bestflow cytometry technology available on the market.Instrument6Design
Software CyView Optical Control of the Flow Process04 SoftwareCyView : Powerful but intuitiveSoftware for CyFlow Cube 6.The easy-to-use CyView data acquisition and data analysis software is your perfectcontrol tool for operating the CyFlow Cube 6 with maximum efficiency.The Windows CyView software integrates instrumentCyView Software Specificationscontrol including acquisition, on- and offline data analysis, Windows based CyView software for routine and research applications multiple language support for CyView software menues editable CyView user environments flow cytometry standard file format (FCS 2.0, 3.0, 3.1) for storage of originalon- and offline compensation into a complete softwarepackage. Predefined and freely adaptable instrument settingsand panels facilitate switching between different applications.CyView is dedicated for all applications in immunophenotyping, microbiology, cell cycle analysis, DNA quantification,ploidy analysis, etc. Data are stored in FCS flow cytometrystandard file format for easy exchange with other analysissoftware. One of the unique features is the digital on- andoffline color crosstalk compensation of the spectral overlapof fluorescence from simultaneously analysed dyes. TheN-color software compensation algorithm allows a correctionof the crosstalk between any parameters without the needof rerunning a sample.CyView optimally supports the True Volumetric AbsoluteCounting feature of the CyFlow Cube 6, displaying particleconcentrations for any subsets of cells, even if defined bya gate at a later time after the acquisition.and evaluated data 8 parameter real-time data acquisition, real-time data analysis,real-time data display 64 calculated parameters plus time parameter one and two parameter histograms and dotplots 64 — 4096 channels resolution for 1P histograms 64/64 — 4096/4096 channels for 2P dotplots linear 3-decade logarithmic 4-decade logarithmic scale (selectable) software-based lin/log transformation single and multiple trigger on any parameter or combinationof parameters (AND/OR) analysis pre-selectable on time, number of events, sample volume multiparameter online crosstalk compensation multiparameter online color gating doublet discrimination DNA cell cycle and DNA peak analysis software-controlled True Volumetric Absolute Counting peak and cluster analysis and statistics real-time sort trigger generation data display templates, plots, channels, regions and calculated resultseditable via XML files all steps of complete analysis runs editable via XML files compensation and XML configurations can be stored separatelyor included in the FCS file connection to well plate/sample tube autoloader multitube panel system with automated acquisition automated data transfer to laboratory information systems (LIS) support of 3rd party flow cytometry software (on request)Several features are in preparation. Technical specifications are subject to change without notice.Software 7
05 TVAC (True Volumetric Absolute Counting)The Optimum in precision and accuracy.Partec True Volumetric Absolute Counting and the CyFlow Cube 6 flow cuvetteensure that cells and particles are analysed and counted with the highestpossible precision and accuracy.The CyFlow Cube 6 analyses concentrations of any particleor cell subpopulation using True Volumetric Absolute Counting (TVAC). This advanced technology is solely based on thefundamental definition of absolute counting i.e.: the particleconcentration (c) is equal to the counted number (n) of cellsin a given volume (v), c n/v. In the CyFlow Cube 6, the volumeis precisely measured directly by mechanical means, ratherthan indirectly with expensive and sometimes problematicbeads, thus eliminating any errors related to varying beadconcentrations or bead aggregations. The CyFlow Cube 6allows the analysis of a fixed volume as defined by the distance between two platinum electrodes. The desired volumecan also be freely selected, based on digital sample speedcontrol by software.StartCountTrue8 Volumetric Absolute CountingStopHighlights of TVACabsolute counts with CV 2%no expenses for calibration beadsno errors related to calibration reduction in cost, time and preparation steps becauseneither reference beads nor hematology referencecounts are required
06 Partec Quartz Flow CuvetteTHE HEART OF THE FLOW CYTOMETer.The CyFlow Cube 6 quartz flow cuvette design is based on Partec’sunique experience having introduced fluorescence-based flow cytometry in 1968.SIDE ScatterHigh NA ObjectiveRed Diode LaserForward ScatterSampleBlue Solid State LaserSheath Fluid (Hydrodynamic Focusing)The design of the CyFlow Cube 6 quartz flow cuvette incor at a time as they pass the laser beam. Due to the opticalporates more than 40 years of Partec experience in handlingand mechan ical design of the Partec flow cuvette, superiorfluids with nanoliter precision. This high-precision analysisresults are guaranteed for all parameters, e.g. coefficients ofis achieved by hydrodynamic focusing of the particles as theyvariation (CVs) of about 2.5% on all fluorescence channels.flow through the cuvette, one optimally — aligned particlePartec Quartz Flow Cuvette 9
07 SpecificationsCyFlow Cube 6 Specifications.Superior performance and state-of-the art technology at a glance.up to 2 light sources simultaneously blue solid state laser:20, 50, 100 mW@488 nmred diode laser: 25, 40 mW@638 nm other laser power and laser wavelengths availableOptics modular optical system with up to 6 opticalparameters with selected PMTs with integratedelectronic preamplifier for FSC, SSC, FL1-FL4standard setup and filterscolor CCD camera for video flow monitor standard objective mount with high numericalaperture immersion gel coupling, e.g. for detectionof weak cytokines (option) separated intermediate image planes foroptimized spatial filtering by diaphragmsElectronicsLength 718 mm15" ScreenHeight 528 mmLight Sourcescleaning system, avoids sample droplets andminimizes cross contamination True Volumetric Absolute Counting based onmechanical volume measurement, no needfor reference particles contamination-free computer controlled precision syringe pump for sample transport andTrue Volumetric Absolute Counting, pumpspeed continuously adjustable from 0–20 µl/s,sheath fluid pressure continuously adjustablefrom 0–800 mbar easily accessible sheath fluid and waste reservoirswith fluid level sensorsreal-time data acquisition, real-time data analysisand real-time data display for detailed specifications please see page 7Length 381 mmHeight 279.5 mm 50 MESF (PE)fluorescence resolution: CV 2%DNA quantification: CV 1% configurations with up to 4 colors,6 optical parameters time parametertransport with sheath fluid sample port with computer controlled BioSafetySoftware Windows based FCM software CyView for parallel signal processing for each of the opticalchannels with 16 bit analog-to-digital converters single and multiple trigger on any parameter orcombination of parameters (AND/OR) individual threshold level settingsLength 381 mmComputer Displaybuilt-in latest industry standard Windows PCintegrated 15" TFT LCD displayCCD video camera for flow monitordual screen setup (optional)keyboard, mouse100 MB/s and 1000 MB/s Ethernet connection DeskJet color printer, b&w or color laser printer(optional), printing via networkDepth 280 mmanalysis of single cells and microscopic particlesscatter particle size range: 50 nm - 200 µmfluorescence sensitivity: 100 MESF (FITC)Flow System synthetic quartz flow cuvette for laminar sampleDepth 280 mmHeight 279.5 mmGeneral compact flow cytometer for automated sequentialTechnical specifications are subject to change without notice. Partec 2013. All rights reserved.Specifications10For in vitrodiagnostic use.
08 CompanyFlow Cytometry made BY PARTEC.Excellence for new applications and increasing requirementsin clinical routine and research applications. Partec 2013. All information is subject to change.More than 40 Years of Experience andProfessional ExpertisePartec (est. 1967) — pioneer in flow cytometry for 45 years —continues this tradition by introducing the newest generationof CyFlow Analysers and CyFlow Sorters featuring innovative computer controlled fluidic systems, modular opticalbench systems with advanced PMTs for all optical channels,state-of-the-art computer and digital electronic technologies as well as real-time data acquisition and real-time datadisplay.Highest Quality WarrantyQuality, performance, precision and cost effectiveness ofPartec instruments and reagents profit from a unique produc tion depth for manufacturing modules and components inoptics, electronics, laser technology, fluidics and mechan ics,employing a modern and sophisticated production line andQuality Managment System with certified highest internationalstandards. This includes research, development, productioncompliant to cGMP, service and customer support.Covering more than 100 countries worldwidewww.partec.com/worldwideCompany 11
Product Fact SheetCyFlow Cube 6Product PictureProductivity valuesHigh-performance, bench-top design withfluidics, built-in PC and a 15" TFT monitorintegratedMain features of CyFlow Cube 6 Product nameConfigurations with up 6 optical parameters(up to 4 colours)488 nm blue laser and 638 nm red laserParticle size: 0.1 – 100 µmFluorescence resolution: CV 2%Fluorescence sensitivity: 100 MESF (FITC) 50MESF (PE)Maximum acquisition rate 15.000 particles/sFlexible system configurationsAutomatic absolute counting by electrodes (TVAC)and syringe controlled volumetric countingOptional CyFlow Robby 6 Autoloading Station forwell plates and tubesStart-up time 5minEasy to use acquisition softwareCyFlow Cube 6 Manufacturer informationSpecifications The CyFlow Cube 6 is manufactured bySysmex Partec GmbH.Sysmex Partec GmbHAm Flugplatz 1302828 ers LightSources Optics Sysmex Partec is an ISO 9001:2008 and ISO 13485:2012certified company. Summary The CyFlow Cube 6 is a compact bench top flowcytometer for analysis of single cells and microscopic particles. The CyFlow Cube 6 with its standardized 2laser configuration system is an optimal solution fordedicated applications. The easy-to-use CyView software provides instrument control, data acquisition and data storage. Furthermore CyFlow Cube 6 offers the TrueVolumetric Absolute Counting (TVAC) feature which allowsdisplaying of particle concentrations for any subsets ofcells without the need of reference beads, even if definedby a gate at a later time after the acquisition.FlowSystem Description5 to 6 optical parameters(3 to 4 colours FSC & SSC)Up to 2 simultaneous light sources(laser)o Blue laser: 50mW @488nmo Red laser: 25 mW @638nmModular optical system with selectedPMTs with integrated electronicpreamplifier for FSC, SSC, FL1-FL4Exchangeable optical filtersStandard objective mount with highnumerical apertureSeparated intermediate image planesfor optimized spatial filtering bydiaphragmsQuartz flow cuvette for laminar sampletransport and hydrodynamic focusingCompletely closed fluidic systemSample port with biosafety cleaningsystemTrue Volumetric Absolute Countingbased on mechanical volumemeasurementComputer controlled precision Syringepump, speed continuously adjustablefrom 0–20 μl/sEasily accessible sheath fluid andwaste reservoirs with fluid levelsensorsSysmex Partec GmbHAm Flugplatz 13, 02828 Görlitz, Germany Phone 49 3581 8746-0 Fax 49 3581 8746-70 info@sysmex-partec.com www.sysmex-partec.comDesign and specifications may be subject to change without notice due to further product development and it is not intended to grant rights or impose obligations.Rev. 001 Date 2015-06-22 ENPage 1 /2
Product Fact Sheet CyFlow Cube 6Electronics Computer Software Dimensions Weight QC functions Interface Operative temperatureOperative humidityParallel signal processing for eachoptical channelSingle and multiple trigger on anyparameter or combination ofparametersIndividual threshold level settings16 bit analog-to-digital convertersBuilt-in Windows PCMicrosoft Windows 7 professional64-bit operating systemIntegrated 15" TFT LCD displayDual screen setup (optional)Keyboard, mouse4 USB ports100MB/s and 1000MB/s EthernetconnectionDeskJet colour printer, printing vianetworkWindows based FCM softwareCyView for real-time data acquisition,real-time data analysis and real-timedata displayEditable CyView user environmentsGuided prime and shut down proceduresEasy experimental template set up(configuration files)Flow cytometry standard data (FCS)format for storage of original andevaluated data1 parameter histograms and dot plots64 — 4096 channels resolution for 1parameter histograms64/64 — 4096/4096 channels for 2parameter dot plotsTime parameterSelectable linear scale or 4 decadelogarithmic scaleSoftware-based lin/log transformationAnalysis pre-selectable on time, numberof events or sample volumeMulti parameter online/offline crosstalkcompensationMulti parameter gating(colour highlighting feature)Compensation can be stored separatelyor included in the FCS fileFCS Express RUO software(Dongle version) for data analysis andreportingStandalone instrument:L 385 mm x W 280 mm x H 290 mmWith Autoloading Station: L 745 mmNoise Electrical SpecificationNominal voltagePower consumption 70 dBA2/II100 – 240 VAC200 VAArticle numberArticle no.CY-S3060R V1 SCY-S3060R V2 SCY-S3060R V3 SCY-S-3080-6Item CyFlow Cube 6Description488/50-5P 638/25-1PCyFlow Cube 6 488/50-5P 488/50-6PCyFlow Cube 6 CyFlow Robby 6 Autoloading StationThis product is intended ‘For Research Use Only’ (RUO).18 kgControl of instrument operationUSB, LAN, video output15-30 C20-85%, non-condensingSysmex Partec GmbHAm Flugplatz 13, 02828 Görlitz, Germany Phone 49 3581 8746-0 Fax 49 3581 8746-70 info@sysmex-partec.com www.sysmex-partec.comDesign and specifications may be subject to change without notice due to further product development and it is not intended to grant rights or impose obligations.Rev. 001 Date 2015-06-22 ENPage 2 / 2
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unIQuE Flow CyToMETRy DESIGn by PARTEC. superior performance. Most Cost-effective solution. the CyFlow Cube 6 from partec opens a new dimension in flow cytometry. high-performance, bench-top design with fully-integrated fluidics, built-in PC and a 15" TFT monitor _ 488nm and
