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SimplyBlue SafeStainSimplyBlue SafeStainFast, Sensitive, and Safe Protein StainingWith SimplyBlue SafeStainprotein staining is: FastBands develop in minutes SensitiveDetect as little as 7 ng of protein SafeNo hazardous acid/alcoholfixatives or destains required
SimplyBlue SafeStain SimplifiesProtein StainingSimplyBlue SafeStain is a uniquely formulated, ready-to-use Coomassie G-250 stain used to visualize protein bands in polyacrylamide gels. WithSimplyBlue , you’ll get the highly sensitive staining results your experimentsrequire–fast. Solution preparation time and expenditure is eliminated,saving you time and money. In addition, staining with SimplyBlue iscompletely non-hazardous and the protocol does not require acid/alcohol fixatives ordestaining solutions. You’ll save money on hazardous material shipping, waste storage, anddisposal charges, and you’ll create a healthier work environment. No other commerciallyavailable protein stain can meet these standards.Simple protocol is fast and flexibleWith SimplyBlue SafeStain, Coomassie staining is as easyhours of destaining time. If you need maximum sensitivityas 1-2-3 (Figure 1). Simply rinse your gel for 15 minutes inand crystal clear background, you can perform an optionalwater, stain with 20 ml ofSimplyBlue SafeStain, andwater-based destain. With SimplyBlue your bands willanalyze your results. A fixative solution is not required fornever fade–even if the gel is left overnight in the stain orany gel format. Bands begin to develop immediately. In asdestain. You don’t have to watch over your gels, freeinglittle as 5 minutes, you can see 1 µg of protein. For fastyour time for more important matters.results, gels can be analyzed directly in the stain, eliminatingFigure 1 - Staining with SimplyBlue SafeStain is as easy as 1-2-312Rinse your gel with water for 5 minutes.Repeat two more times.Stain your gel with 20 ml of SimplyBlue SafeStain for 1 hour*. Bands develop inminutes.3Analyze your gel directly in the stain, orcontinue with the optional water-baseddestaining protocol to achieve maximumsensitivity and crystal-clear background.*Although 1 µg of most proteins can be detected within 5 minutes, the SimplyBlue Basic Protocol recommends staining for 1 hour to ensure maximum dye saturation of allproteins and concentrations.Toll Free: 800 955 6288
SimplyBlue SafeStainVersatile Staining ReagentCompatible with the NuPAGE Gel SystemThe patented NuPAGE Gel system offers high-performanceCoomassie stains (Figure 2). With SimplyBlue , less than 7 ng ofprotein separation in a neutral pH environment. This results inreduced BSA can be detected–that’s at least two times greaterreduced protein modifications and increased gel stability to ensuresensitivity than with other stains. If you’re not using NuPAGE accurate separation results and provide a 12-month shelf-life.Gels don’t worry. SimplyBlue will give you fast, sensitive resultsSimplyBlue SafeStain is specifically designed to achieve maximumwith all polyacrylamide gels including Tris-Glycine, Tricine, IEF,staining performance withNuPAGE Gels.NuPAGE Gels stainedwith SimplyBlue demonstrate faster band development and moreZymogram, and native-PAGE gels, as well as with PVDFmembranes.sensitive staining results than with other commercially availableFigure 2 - Comparison of NuPAGE Novex Bis-Tris 4-12% Gels stained with SimplyBlue SafeStain or GelCode BlueA. Gel stained with SimplyBlue 12345B. Gel stained with GelCode Blue678910NuPAGE Novex Bis-Tris 4-12% Gel stained with SimplyBlue for1 hour, then destained overnight in a water-based destain. As little as 2ng reduced BSA can be detected (Indicated by the arrow in lane 10).The following samples were loaded on each gel:LaneLaneLaneLaneLane1:2:3:4:5:1 µg reduced BSA10 µl Mark12 Standard16 ng reduced BSA8 ng reduced BSA7 ng reduced BSALaneLaneLaneLaneLane6: 6 ng reduced BSA7: 5 ng reduced BSA8: 4 ng reduced BSA9: 3 ng reduced BSA10: 2 ng reduced BSA12345678910NuPAGE Novex Bis-Tris 4-12% Gel stained with GelCode Blue for1 hour, then destained overnight in water. Even the 16 ng reducedBSA band is not visible (Indicated by the arrow in lane 3).
SimplyBlue SafeStainSensitive Staining for MaximumPerformanceDetect as little as 7ng of proteinSimplyBlue SafeStain combines a unique colloidalAfter just 5 minutes in the SimplyBlue SafeStain, 1 mgCoomassie G-250 staining solution with an optimizedof protein can be detected. With additional water-basedstaining protocol to achieve the highest sensitivity of anydestaining, 7 ng or less can be detected. In addition,commercially available Coomassie stain. The gels inthere is no loss in sensitivity when the gel is leftFigure 3 demonstrate the superior staining resultsovernight in the destain. With SimplyBlue , you’ll get theachieved withSimplyBlue over other Coomassie stains.most sensitive staining results.Figure 3 - Greater sensitivity with SimplyBlue SafeStainSimplyBlue SafeStainA1 234567 8 9 10GelCode BlueD1 234567 8 9 10Coomassie R-250 StainG1 234567 8 9 10The following samples wereelectrophoresed on NuPAGE Novex Bis-Tris 4-12% Gelsand then stained with eitherAfter5 minutesin stainSimplyBlue SafeStain,GelCode Blue, or CoomassieR-250 following manufacturerrecommended protocols. Sensitivity: 1 µg protein Mark12 clearly visibleB1 23456 Sensitivity: 6 µg protein Mark12 not visible7 8 9 10E1 234567 8 9 10 Sensitivity: 1 µg protein Mark12 not visible High backgroundH1 234567 8 9 10Items to note are listed beloweach gel. Arrows indicateachieved sensitivity.Lane 1: 6 µg protein mixLane 2: 1 µg rabbit IgGLane 3: 1 µg reduced BSALane 4: 5 µg E. coli lysateAfter2 hours indestainLane 5: 20 ng reduced BSALane 6: 10 ng reduced BSALane 7: 7 ng reduced BSALane 8: 3 ng reduced BSALane 9: 10 µl Mark12 Standard Water-based destain Sensitivity: 7 ng reduced BSAC1 23456 789 10 Water destain Sensitivity: 20 ng reduced BSAF12 3456 7 8 9 10 Methanol/acetic acid destain Sensitivity: 20 ng reduced BSA High BackgroundI1 23456Lane 10: 5 µl Mark12 Standard7 8 9 10Afterovernightin destain Water-based destain Sensitivity: 7 ng reduced BSA No loss in sensitivity withovernight water-based destaining Water destain Sensitivity: 20 ng reduced BSA Loss in sensitivity with overnightwater destaining Methanol/acetic acid destain Sensitivity: 10 ng reduced BSAwww.invitrogen.com
SimplyBlue SafeStainSafe, Non-hazardous StainingNo acid or alcohol requiredUnlike traditional R-250 and some G-250 Coomassiesave money on hazardous shipping, waste storage, andstains, SimplyBlue SafeStain does not require methanoldisposal fees. Additionally, SimplyBlue SafeStain hasor acetic acid fixatives or destains. The risks ofbeen independently tested for safety and confirmed to behazardous exposure and unpleasant odors are eliminated,non-hazardous, non-toxic, non-flammable, and non-providing a healthier lab environment. There are nocorrosive (Table 1). In most areas, SimplyBlue can beHazMat charges associated with SimplyBlue , so you’llsafely disposed down the drain*.Table 1 - SimplyBlue SafeStain is confirmed non-hazardousAnalysisMethodResultsIgnitabilityEPA 1010Non-hazardous ( 144 F)CorrosivityEPA 150.1Non-hazardous ( pH 2.1)Corrosivity(by Corrositex )DOT-E 10904Non-hazardousReactivity(Cyanide and Sulfide)EPA 9010B/9014EPA 9030ANo Reactivity DetectedAquatic Toxicity(fathead minnow; definitive,CAC, Title 22)CA Fish and GameNon-hazardous* SimplyBlue SafeStain has been extensively tested for safety. Ogden Environmental and EnergyServices Co., Inc. San Diego, CA U.S.A, has independently confirmed these tests. Check the MSDSand your local authorities for special disposal requirements in your area.www.invitrogen.com
SimplyBlue SafeStainConvenient PackagingSupplied ready-to-useSimplyBlue SafeStain is packaged in a 1 L bottle containingThe SimplyBlue staining reagent is guaranteed to be stable1X, ready-to-use staining solution. Simply follow theand provide maximum performance for 6 months. As aninstructions conveniently located on the bottle to achieveadded convenience, SimplyBlue can be stored on yourhigh-quality staining results. We also provide protocols forbench top at room temperature, so it’s within reach whenachieving maximum sensitivity with various gel formats.you need it.Order your next favorite blue todayFor fast, sensitive, and safe protein staining, get SimplyBlue SafeStain. You’ll save time, money, and get superiorsensitivity in all your protein polyacrylamide gel analyses.Order today and make SimplyBlue your next favorite blue.DescriptionSimplyBlue SafeStainSimplyBlue SafestainQuantity1 L*3.5LCat. No.LC6060LC6065*Sufficient reagent is supplied to stain 50 mini-gels.SimplyBlue is a trademark of Invitrogen Corporation. NuPAGE is a registered trademark of Invitrogen Corporation and is covered under U.S. Patent No. 5,578,180 and 6,059,948. Coomassie is a registered trademark of Imperial Industries, PLC. GelCode is a registered trademark of PIERCE Chemical Company. Corrositex is a registered trademark of InVitro International.1600 Faraday AvenueCarlsbad, California 92008 Toll Free: 800 955 6288 P:760 603 7200 F: 760 603 7201 www.invitrogen.com 2001 Invitrogen Corporation. Printed in the U.S.A. Reproduction forbidden without permission. All prices are in U.S. dollars and are subject to change.713-01979 110101Updated 021902
SimplyBlue SafeStain Simplifies Protein Staining With SimplyBlue SafeStain, Coomassie staining is as easy as 1-2-3 (Figure 1). Simply rinse your gel for 15 minutes in water, stain with 20 ml of SimplyBlue SafeStain, and analyze your results. A fixative solution is not required for any gel format. Bands begin to develop immediately. In as
