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Comparison of Applied Biosystems TaqMan ️ andRoche reagents on the LightCycler 480 II Real-Time PCR Detection SystemNovember 12, 20181The world leader in serving science
Applied Biosystems TaqMan Reagents on Roche LC480 II systemObjective:Compare the performance of TaqMan ️ Master Mixes and Roche reagents using TaqMan ️ Gene Expression Assays on the RocheLightCycler 480 II real time PCR detection system. TaqMan Fast Advanced Master Mix with TaqMan Gene ExpressionAssays in singleplex and multiplex experiments Sensitivity Dynamic Range Singleplex vs. Duplex Gene Expression Applied Biosystems TaqPath 1-Step Multiplex Master Mix withTaqMan Gene Expression Assays Sensitivity Dynamic Range Singleplex vs. 4-plex Gene ExpressionFor Research Use Only. Not for use in diagnostic procedures. TaqPath 1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018 Thermo FisherScientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of RocheMolecular Systems, Inc., used under permission and license. Roche is a trademark of Hoffman-La Roche., and LightCycler is a registered trademark of Roche Diagnostics GmbH, which arenot affiliated with Thermo Fisher Scientific. The statements herein have not been reviewed or endorsed by Roche or its subsidiaries.
TaqMan Fast Advanced vs LightCycler Probes Master ComparisonTaqMan Fast Advanced master mix outperforms the LCProbes Master mix on the Light Cycler 480 II systemAttributeTaqMan Fast AdvancedMaster MixLightCycler ProbesMaster7 Log dynamic range Sensitivity @ 1 copy Performance in multiplex -Includes UNG for carry over contamination -Faster by 10 minFast72 hr24 hrRun timeBenchtop stabilityFor Research Use Only. Not for use in diagnostic procedures. TaqPath 1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018 Thermo FisherScientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of RocheMolecular Systems, Inc., used under permission and license. Roche is a trademark of Hoffman-La Roche., and LightCycler is a registered trademark of Roche Diagnostics GmbH, which arenot affiliated with Thermo Fisher Scientific. The statements herein have not been reviewed or endorsed by Roche or its subsidiaries.
TaqMan Fast Adv. and LC 480 Probes Master ComparisonTaqMan ️ Fast Advanced Master MixOn the LightCycler 480System IIVSLightCycler Probes MasterFor Research Use Only. Not for use in diagnostic procedures. TaqPath 1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is aregistered trademark of Roche Molecular Systems, Inc., used under permission and license. Roche is a trademark of Hoffman-La Roche., and LightCycler is a registeredtrademark of Roche Diagnostics GmbH, which are not affiliated with Thermo Fisher Scientific. The statements herein have not been reviewed or endorsed by Roche or its
TaqMan Fast Advanced Master Mix: Gene Expression MultiplexTaqMan ️ Fast Advanced Master Mix outperforms Roche LC 480Probes Master Mix in multiplexed reactions on the Roche LightCycler 480 II*. 50ng Ave. Cq valueSimplex vs DuplexTarget Assay18S*Multiplex and singleplexreactions performed with 50ngcDNA with TaqMan GeneExpression Assays in 5replicatesFor Research Use Only. Not for use in diagnostic procedures. TaqPath 1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018 ThermoFisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registeredtrademark of Roche Molecular Systems, Inc., used under permission and license. Roche is a trademark of Hoffman-La Roche., and LightCycler is a registered trademark of RocheDiagnostics GmbH, which are not affiliated with Thermo Fisher Scientific. The statements herein have not been reviewed or endorsed by Roche or its subsidiaries.
TaqMan Fast Advanced Master Mix: SensitivityLightCycler 480 Probes MasterTaqMan Fast Advanced Master MixTaqMan Fast Advancedmaster mix demonstratessensitivity down to 1copy on the LightCycler 480 II**Multiplex and singleplexreactions performed with50ng cDNA with TaqManGene Expression Assays in 5replicatesFor Research Use Only. Not for use in diagnostic procedures. TaqPath 1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is aregistered trademark of Roche Molecular Systems, Inc., used under permission and license. Roche is a trademark of Hoffman-La Roche., and LightCycler is a registeredtrademark of Roche Diagnostics GmbH, which are not affiliated with Thermo Fisher Scientific. The statements herein have not been reviewed or endorsed by Roche or its
TaqMan Fast Advanced Master Mix: Dynamic RangeTaqMan Fast Advanced master mix yields broad linear dynamic rangeand reliable quantitation at low copy numberLightCycler 480 Probes MasterTaqMan Fast Advanced Master MixAmplification plots from an 8-log dilution series of 50 ng / µL human cDNA amplified in 5 replicate reactions with an 18S rRNA TaqMan Gene Expression Assay on a RocheLightCycler 480 II Real-Time PCR Detection System. LightCycler 480 Probes Master (left) was compared to TaqMan Fast Advanced Master Mix (right). Note the moreconsistent results with TaqMan Fast Advanced Master Mix at the lowest concentrationFor Research Use Only. Not for use in diagnostic procedures. TaqPath 1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018 Thermo FisherScientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of RocheMolecular Systems, Inc., used under permission and license. Roche is a trademark of Hoffman-La Roche., and LightCycler is a registered trademark of Roche Diagnostics GmbH, whichare not affiliated with Thermo Fisher Scientific. The statements herein have not been reviewed or endorsed by Roche or its subsidiaries.
TaqPath 1-Step Multiplex Master Mix Comparison TaqPath 1-Step Multiplex Master Mix(No ROX)On the LightCycler 480System IILightCycler Multiplex RNA VirusMaster MixFor Research Use Only. Not for use in diagnostic procedures. TaqPath 1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018 ThermoFisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registeredtrademark of Roche Molecular Systems, Inc., used under permission and license. Roche is a trademark of Hoffman-La Roche., and LightCycler is a registered trademark of RocheDiagnostics GmbH, which are not affiliated with Thermo Fisher Scientific. The statements herein have not been reviewed or endorsed by Roche or its subsidiaries.
TaqPath 1-Step Multiplex vs LC Multiplex RNA Virus ComparisonTaqPath 1-Step Multiplex Master Mix outperforms theLightCycler Multiplex RNA Virus Master on theLight Cycler 480 IIAttributeTaqPath 1-Step MultiplexMaster MixLC Multiplex RNAVirus MasterDynamic range -Sensitivity -Performance in multiplex -PCR efficiency -Includes UNG for carry over contamination -General Purpose Reagent -Run time For Research Use Only. Not for use in diagnostic procedures. TaqPath 1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018 ThermoFisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registeredtrademark of Roche Molecular Systems, Inc., used under permission and license. Roche is a trademark of Hoffman-La Roche., and LightCycler is a registered trademark of RocheDiagnostics GmbH, which are not affiliated with Thermo Fisher Scientific. The statements herein have not been reviewed or endorsed by Roche or its subsidiaries.
TaqPath 1-Step Multiplex Master Mix: Dynamic range TaqPath 1-Step Multiplex Master Mix detected at all copy levels on the RocheLightCycler 480 II*No amplificationNo amplification*Thirty-twoTaqMan GeneExpressionAssays wererun in both 4plex (5replicates) andin simplex (3replicates).
TaqPath 1-Step Multiplex: Gene Expression Multiplex TaqPath 1-step Multiplex Master Mix provides consistent, reliable resultsin singleplex and multiplex gene expression on the Roche LightCycler 480 II**Thirty-two TaqManGene ExpressionAssays were run inboth 4-plex (5replicates) and insimplex (3 replicates).For Research Use Only. Not for use in diagnostic procedures. TaqPath 1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018 ThermoFisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registeredtrademark of Roche Molecular Systems, Inc., used under permission and license. Roche is a trademark of Hoffman-La Roche., and LightCycler is a registered trademark ofRoche Diagnostics GmbH, which are not affiliated with Thermo Fisher Scientific. The statements herein have not been reviewed or endorsed by Roche or its subsidiaries.
LightCycler Multiplex RNA Virus: Gene Expression Multiplex LightCycler Multiplex RNA Virus Master Mix shows inconsistent resultsbetween singleplex and multiplex gene expression on the Roche LightCycler 480 II**Thirty-two TaqManGene ExpressionAssays were run in both4-plex (5 replicates) andin simplex (3 replicates).For Research Use Only. Not for use in diagnostic procedures. TaqPath 1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018 Thermo FisherScientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of RocheMolecular Systems, Inc., used under permission and license. Roche is a trademark of Hoffman-La Roche., and LightCycler is a registered trademark of Roche Diagnostics GmbH, whichare not affiliated with Thermo Fisher Scientific. The statements herein have not been reviewed or endorsed by Roche or its subsidiaries.
TaqPath 1-Step Multiplex Master Mix: Multiplexing TaqPath 1-step Multiplex outperforms LightCycler Multiplex RNAVirus Master Mix in multiplex qPCR on the Roche LightCycler 480 IIFor Research Use Only. Not for use in diagnostic procedures. TaqPath 1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is aregistered trademark of Roche Molecular Systems, Inc., used under permission and license. Roche is a trademark of Hoffman-La Roche., and LightCycler is a registeredtrademark of Roche Diagnostics GmbH, which are not affiliated with Thermo Fisher Scientific. The statements herein have not been reviewed or endorsed by Roche or itssubsidiaries.
TaqPath 1-Step Multiplex Master Mix: Sensitivity TaqPath 1-step Multiplex shows superior sensitivity compared toLightCycler Multiplex RNA Virus Master Mix on the Roche LightCycler 480 II*Ave. xRNA RNAVirus tepMultiplexMasterMasterMix Mix252015105 Ave Cq of 50ng (TaqPath vs. LightCycler)*Thirty-two TaqMan GeneExpression Assays were run inboth 4-plex (5 replicates) andin simplex (3 replicates).For Research Use Only. Not for use in diagnostic procedures. TaqPath 1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018 Thermo FisherScientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of RocheMolecular Systems, Inc., used under permission and license. Roche is a trademark of Hoffman-La Roche., and LightCycler is a registered trademark of Roche Diagnostics GmbH, whichare not affiliated with Thermo Fisher Scientific. The statements herein have not been reviewed or endorsed by Roche or its subsidiaries.
TaqPath 1-Step Multiplex: PCR Efficiency TaqPath 1-step Multiplex master mix shows superior PCR efficiencycompared to LightCycler Multiplex RNA Virus Master Mix on the Roche LightCycler 480 II*180PCR Eff. (%)16014012010080604020LightCycler MultiplexRNA Virus masterTaqPath 1-step MultiplexMaster Mix0*Thirty-two TaqMan GeneExpression Assays were runin both 4-plex (5 replicates)and in simplex (3 replicates).For Research Use Only. Not for use in diagnostic procedures. TaqPath 1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018 Thermo FisherScientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of RocheMolecular Systems, Inc., used under permission and license. Roche is a trademark of Hoffman-La Roche., and LightCycler is a registered trademark of Roche Diagnostics GmbH, whichare not affiliated with Thermo Fisher Scientific. The statements herein have not been reviewed or endorsed by Roche or its subsidiaries.
Conclusions and Next Steps Applied Biosystems TaqMan / TaqPath assays and reagents outperform the Roche LightCycler reagents on the Roche LightCycler 480 II qPCR instrument. TaqMan Fast Advanced Master Mix shows better reproducibility, dynamic range and PCRefficiency on the LightCycler 480 II for gene expression singleplex and multiplex assays thanLightCycler Probe product/4444556 TaqPath 1-Step Multiplex Master Mix demonstrates improved dynamic range, PCRefficiency, and sensitivity than LightCycler Multiplex RNA Virus Master Mix on RocheLightCycler 480 II for gene expression for both simplex and multiplex applicationshttps://www.thermofisher.com/taqpath To learn more about Applied Biosystems qPCR master mixes or to request a free sample,please visit www.thermofisher.com/qpcrmm Did you know TaqMan assays are guaranteed to perform? To learn more about TaqManassays or purchase an extra small size, please visit www.thermofisher.com/taqmanFor Research Use Only. Not for use in diagnostic procedures. TaqPath 1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018 Thermo FisherScientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of RocheMolecular Systems, Inc., used under permission and license. Roche is a trademark of Hoffman-La Roche., and LightCycler is a registered trademark of Roche Diagnostics GmbH, whichare not affiliated with Thermo Fisher Scientific. The statements herein have not been reviewed or endorsed by Roche or its subsidiaries.
Appendix TaqMan Fast Advanced Master Mix product information TaqPath 1-Step Multiplex Master Mix product information Thermal cycling conditionsFor Research Use Only. Not for use in diagnostic procedures. TaqPath 1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018 Thermo FisherScientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of RocheMolecular Systems, Inc., used under permission and license. Roche is a trademark of Hoffman-La Roche., and LightCycler is a registered trademark of Roche Diagnostics GmbH, whichare not affiliated with Thermo Fisher Scientific. The statements herein have not been reviewed or endorsed by Roche or its subsidiaries.
TaqMan Fast Advanced Master MixTaqMan Fast Advanced Master Mix has beendesigned to match or exceed the performance ofstandard master mixes, delivering shorter run times( 40 minutes) with results equal to or better thanwhat is achieved today. Best-in-class performance Enhanced benchtop stability Optimization for multiplexing Reduced run times on fast and standard instrumentation Includes UNGVerified with TaqMan Assays for gene expression and microRNAs,and TaqMan array microfluidic cardsUse with:TaqMan GeneExpressionAssaysandTaqManAdvancedmiRNA AssaysFor Research Use Only. Not for use in diagnostic procedures. TaqPath 1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018 Thermo FisherScientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of RocheMolecular Systems, Inc., used under permission and license.
TaqPath 1-Step Multiplex Master MixA 1-step RT-qPCR master mix optimizedspecifically for multiplex applications thatdelivers superior performance and enhanceddata confidence. Inhibitor tolerant Excellent sensitivity andreproducibility Optimized for multiplexing Easy-to-use 4X mixManufactured in an ISO 13485 certified facilityTaqPath 1-StepMultiplex Master Mixincludes MUSTANG PURPLE passive reference dyeTaqPath 1-StepMultiplex Master Mix(No ROX)no passive reference dye19General purpose reagent. For Laboratory Use.
Thermal Cycling ConditionsLightCycler 480 ProbeMasterTaqMan FastAdvanced Master MixStandardTaqMan FastAdvanced Master MixFast384 well plate384 well plate384 well plate# of Cycles454545Ramp RateLC 480 II default *LC 480 II default *LC 480 II default *5:00 @ 95 0:20 @ 95 0:20 @ 95 Denature0:10 @ 95 0:15 @ 95 0:03 @ 95 Anneal0:30 @ 50 1:00 @ 60 0:30 @ 60 Extend0:01 @ 72 --0:10 @ 40 -- 58 min 79 min 47 minFormatPre-IncubationEnzyme ActivationAmplificationCoolingTotal Run Time* 384 well plate default ramp rate (up 4.8 C / down 2.5 C )For Research Use Only. Not for use in diagnostic procedures. TaqPath 1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018 Thermo FisherScientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of RocheMolecular Systems, Inc., used under permission and license. Roche is a trademark of Hoffman-La Roche., and LightCycler is a registered trademark of Roche Diagnostics GmbH, whichare not affiliated with Thermo Fisher Scientific. The statements herein have not been reviewed or endorsed by Roche or its subsidiaries.
Thermal Cycling ConditionsLightCycler Multiplex RNA VirusMasterTaqPath 1-StepMultiplex MasterMix384 well plate384 well plate# of Cycles4545Ramp RateLC 480 II default *LC 480 II default *UNG incubationN/A2:00 @ 25 C**Pre-Incubation10:00 @ 50 C10:00 @ 53 CEnzyme Activation0:30 @ 95 C2:00 @ 95 CDenature0:05 @ 95 C0:03 @ 95 CAnneal0:30 @ 60 C0:30 @ 60 C0:30 @ 40 C0:10 @ 40 C 60 min 60 min ( 2 min UNGincubation)FormatAmplificationCoolingTotal Run Time21*384 well plate default ramp rate (up 4.8 C / down 2.5 C )**on the bench (the lowest block temp of LC480II is 37 C
TaqPath 1-step Multiplex outperforms LightCycler Multiplex RNA Virus Master Mix in multiplex qPCR on the Roche LightCycler 480 II TaqPath 1-Step Multiplex Master Mix: Multiplexing For Research Use Only. Not for use in diagnostic procedures. TaqPath1-Step Multiplex Master Mix is a General Purpose Reagent for Laboratory Use. 2018
