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MACSQuant Flow CytometersThe future of automation
At the heart of intelligent designThe first MACSQuant Analyzer was engineeredto enable demanding and diverse flow cytometryapplications of researchers around the world.At Miltenyi Biotec, we have since continued toexpand our instrument portfolio to meet the evergrowing complexities in cell analysis. Innovation isat the core of each MACSQuant Instrument, offeringautomated flow cytometry solutions for a diversearray of appliations.Wondering which MACSQuant Flow Cytometer isthe best fit for your lab? Explore your options in theMACSQuant family of flow cytometers. MACSQuant Analyzer 10automating and standardizing key assays MACSQuant Analyzer 16increasing parameters with more colors MACSQuant Xhigh-throughput applications fromstreamlined fluidics and sample processing MACSQuant VYBred fluorescent protein analysis andoptimized FITC and PE applicationsAutomation featuresAutomated acquisition Automated labeling Automated start up, shutdown, cleaning Automated compensation Optics10 parameter16 parameter 405 nm laser 488 nm laser 561 nm laser640 nm laser 405 nm side scatter488 nm side scatter Sample processingNeedle-arm vibration mixing Orbital sample mixingPipette sample mixing 24-tube rack processing 96-well plate processing 384-well plate processing High-throughput plate screening Sample cooling (chill racks) Low carryover: standard mode 0.01% Absolute cell counting Rare cell enrichment Remote support Unique featuresMulti-instrument alignment 21 CFR Part 11 compliant software
Reliable reagents REAfinity Recombinant Antibodiesfor reproducible results inflow cytometry applications Vio and VioBright Dyes withsuperior mean fluorescence intensityand high stain indicesCustomized solutions Antibodies and conjugates tailoredto your specifications Personalized multicolor antibodycocktails Custom 'Express Mode' development,including pre-set acquisition, algorithmbased gating, and analysis templates21 CFR Part 11 software packageSeamless data submission toregulatory agencies ensured by: Audit trail Analysis reports with e-signature User management system accordingto 21 CFR part 11Support and service Remote support at your fingertipsvia MACSQuant live support portal On- and offsite training programs Application support Comprehensive service offeringsincluding IQOQ
MACSQuant Analyzer 10Your trusted partner in automated flow cytometryThe MACSQuant Analyzer 10 brings to life theversatility and power required for modern flowcytometry applications. Whether mining for rare cells,analyzing the efficiency of your cell manufacturingprocess or investigating signaling pathways, you areequipped for the task at hand.Truly hands-free operationWith a range of automated features, theMACSQuant Analyzer 10 lays the foundation for trueautomation present in all instruments belonging tothe MACSQuant Family of flow cytometers.Automated featuresof the MACSQuant FamilyApplication highlight: Automated assays In-process and quality control Cellular therapy, i.e., chimeric antigenreceptor (CAR) T cell manufacturing Toxicological assays Whole blood analysis Cell counting Rare cell analysis Small particles / exosomes Cytokine arraysStartup, cleaning,and shutdownCompensationLabelingCalibration andquality control (QC)MACSQuant Analyzer 10 optical bench405 nm488 nm640 nm450/50 nmVioBlue 488/10 nmFSC655–730 nmAPC525/50 nmVioGreen 488/10 nmSSC750 nm LPAPC-Vio 770525/50 nmFITC585/40 nmPE655–730 nmPerCP-Vio 700750 nm LPPE-Vio 770
MACSQuant XReliable high-throughput flow cytometryEngineered from the ground up as a compact flowcytometer, the MACSQuant X was made to providespeed and reliability in high-throughput screening.It allows you to get more data, in less time, with lesseffort. Perfect for laboratories that want to maximizeefficiency, while enjoying the certainty of gettingreliable results from every tube, 96- or 384-wellplate. The MACSQuant X has been designed tomeet high-throughput cell analysis needs for themost challenging large-scale screening settings.Technology highlight:Integration with liquid handling systems Robust flow cytometer capable of processingup to 9,200 samples per day(96-well plate within 15 minutes,384-well plate in under 60 minutes) Seamless integration into a varietyof liquid handling systemsNumber of eventsOrbital and needle mixing ensure consistency and accuracyin assay performance and cell counting.4,0003,0002,0001,0000Wells 1–3841384Figure 1: Fast and reliable processing of 96- and 384-well plates.MACSQuant X optical bench405 nm488 nm640 nm450/50 nmVioBlue 488/10 nmFSC655–730 nmAPC525/50 nmVioGreen 488/10 nmSSC750 nm LPAPC-Vio 770525/50 nmFITC585/40 nmPE655–730 nmPerCP-Vio 700750 nm LPPE-Vio 770
MACSQuant Analyzer 16Colorful new possibilities in automated flow cytometryExpanding fully automated data acquisition to16-parameters is what the MACSQuant Analyzer 16is all about. The instrument’s compact designis suited for basic research as well as advancedimmune monitoring applications. It provides userswith the flexibility and customization known fromthe MACSQuant Analyzer 10, with an additional sixfluorescent channels to meet the increasing demandsof modern laboratories.Unique features Expanded fluorescent channels: Amplify your datawith up to 16 parameters, while using 40 % lesssample material Violet side scatter: Enhance your small particleanalysis with the optimal laser line.Technology highlight: More acquisitionparameters and multi-instrument alignment Explore more cell subsets and their activationstatus by utilizing a broader range offluorochrome and antibody options Improved consistency and reproducibility byeasy aligning of instrument settings forintra- and inter-lab assays or applications.Down the hall or around the world!MACSQuant Analyzer 16 optical bench405 nm488 nm640 nm405/10 nmSSC488/10 nmFSC667/30 nmAPC450/50 nmVioBlue 525/50 nmFITC725/40 nmAlexa Fluor 700525/50 nmVioGreen 579/34 nmPE785/62 nmAPC-Vio 770579/34 nmBV 570 615/20 nmPE-Vio 615615/20 nmBV 605 667/30 nmPerCP667/30 nmBV 650 725/40 nmPerCP-Vio 700785/62 nmPE-Vio 770
MACSQuant VYBYour benchmark for fluorescent protein flow cytometryOn top of the performance, automation, compactnessand convenience associated with the MACSQuant Familyof flow cytometers, the MACSQuant VYB comes witha uniquely configured optical bench, featuring violet,yellow, and blue lasers. It is a perfect match for labsutilizing fluorescent protein reporters or for labs thatwant to simply use PE and FITC conjugates.Technology highlight: Finding the right fitfor fluorescent proteins with the yellow laser Fully utilize fluorescent proteins and reporterapplications that require the Violet, Blue orYellow laser such as mCherry, GFP and CFP(figure 2)CFPeGFPYFPmCherrymKate 2PIFigure 2: The MACSQuant VYB can detect a wide range of fluorescentproteins. Histograms of cells transfected with CFP, GFP, YFP (top row),mCherry, and mKate 2 (bottom row) are easily distinguished from nontransfected cells (shown in purple). GFP and YFP are often difficult todistinguish using flow cytometry due to similarities in their emissionspectra. With the MACSQuant VYB, YFP (shown in yellow) and GFP(shown in green) cells are clearly distinguishable when observed in thePI channel (bottom right). Excite and analyze APC and APC tandems Minimize compensation for FITC / GFP andPE conjugates (figure 3)PEAB10³10³10²10²10¹10¹10-1-1 0 1PE Optimal excitation of PE and PE tandem dyes10¹10²10³10-1-1 0 1GFP10¹10²10³GFPFigure 3: Cells expressing GFP were stained using a PE-conjugatedantibody against a dim marker and analyzed using the MACSQuantVYB. Excitation of both PE and GFP with the blue 488 nm laser onlyminimally resolves the two signals (A). In comparison, excitation of PEby the yellow 561 nm laser and of GFP by the blue 488 nm laser fullyresolves these two signals without the need for any compensations (B).MACSQuant VYB optical bench405 nm488 nm561 nm452/45 nmVioBlue , BFP525/50 nmGFP, FITC561/4 nmFSC525/50 nmVioGreen 593–650 nmPI, LSS-mKate561/4 nmSSC586/15 nmPE615/20 nmmCherry, PE-Vio 615661/20 nmmKate, APC750 nm LPPE-Vio 770, APC-Vio 770
miltenyibiotec.com/macsquantThe MACSQuant XReliable high-throughput flow cytometryUSA/CanadaMiltenyi Biotec Inc.2303 Lindbergh StreetAuburn, CA 95602, USAPhone 800 FOR MACSPhone 1 530 888 8871Fax 1 877 591 1060macs@miltenyibiotec.comAustraliaMiltenyi BiotecAustralia Pty. Ltd.Unit 11, 2 Eden Park DriveMacquarie Park, NSW 2113AustraliaPhone 61 2 8877 7400Fax 61 2 9889 5044macs@miltenyibiotec.com.auBeneluxMiltenyi Biotec B.V.Sandifortdreef 172333 ZZ LeidenThe Netherlandsmacs@miltenyibiotec.nlCustomer serviceThe NetherlandsPhone 0800 4020120Fax 0800 4020100Customer service BelgiumPhone 0800 94016Fax 0800 99626Customer service LuxembourgPhone 800 24971Fax 800 24984ChinaMiltenyi Biotec Technology &Trading (Shanghai) Co., Ltd.Room 401No. 1077, Zhangheng RoadPudong New Area201203 Shanghai, P.R. ChinaPhone 86 21 6235 1005Fax 86 21 6235 0953macs@miltenyibiotec.com.cnFranceMiltenyi Biotec SAS10 rue Mercoeur75011 Paris, FrancePhone 33 1 56 98 16 16Fax 33 1 56 98 16 17macs@miltenyibiotec.frItalyMiltenyi Biotec S.r.l.Via Paolo Nanni Costa, 3040133 BolognaItalyPhone 39 051 6 460 411Fax 39 051 6 460 499macs@miltenyibiotec.itJapanMiltenyi Biotec K.K.NEX-Eitai Building 5F16-10 Fuyuki, Koto-kuTokyo 135-0041, JapanPhone 81 3 5646 8910Fax 81 3 5646 8911macs@miltenyibiotec.jpNordics and BalticsMiltenyi Biotec Norden ABScheelevägen 17223 70 LundSwedenmacs@miltenyibiotec.seCustomer service SwedenPhone 0200 111 800Fax 046 280 72 99 Customer service DenmarkPhone 80 20 30 10Fax 46 46 280 72 99 Customer serviceNorway, Finland, Iceland,and Baltic countriesPhone 46 46 280 72 80Fax 46 46 280 72 99SingaporeMiltenyi Biotec Asia Pacific Pte Ltd438B Alexandra Road, Block BAlexandra Technopark#06-01Singapore 119968Phone 65 6238 8183Fax 65 6238 0302macssg@miltenyibiotec.com.sgSouth KoreaMiltenyi Biotec Korea Co., Ltd.Arigi Bldg. 8F562 Nonhyeon-roGangnam-guSeoul 06136, South KoreaPhone 82 2 555 1988Fax 82 2 555 8890macs@miltenyibiotec.co.krSwitzerlandMiltenyi Biotec Swiss AGGibelinstrasse 274500 SolothurnSwitzerlandPhone 41 32 623 08 47Fax 49 2204 85197macs@miltenyibiotec.chUnited KingdomMiltenyi Biotec Ltd.Almac House, Church LaneBisley, Surrey GU24 9DR, UKPhone 44 1483 799 800Fax 44 1483 799 SpainMiltenyi Biotec S.L.C/Luis Buñuel 2Ciudad de la Imagen28223 Pozuelo de Alarcón (Madrid)SpainPhone 34 91 512 12 90Fax 34 91 512 12 91macs@miltenyibiotec.esMiltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact.Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.MACS, and the MACS logo are registered trademarks or trademarks of Miltenyi Biotec and/or its affiliates in various countries worldwide.Copyright 2020 Miltenyi Biotec and/or its affiliates. All rights reserved.130-118-920.04Germany/AustriaMiltenyi Biotec B.V. & Co. KGFriedrich-Ebert-Straße 6851429 Bergisch GladbachGermanyPhone 49 2204 8306-0Fax 49 2204 85197macs@miltenyibiotec.de
Robust flow cytometer capable of processing up to 9,200 samples per day (96-well plate within 15 minutes, 384-well plate in under 60 minutes) Seamless integration into a variety of liquid handling systems Figure 1: Fast and reliable processing of 96- and 384-well plates. Orbital and needle mixing ensure consistency and accuracy
