WIXLIB

CANDIDATE BRIEFCryo-Electron Microscopy Scientist or Senior Cryo-Electron MicroscopyScientist, Faculty of Biological SciencesSalary: Grade 7 ( 33,797– 40,322 p.a.) orGrade 8 ( 41,526 – 49,553 p.a.)Reference: FBSBY1045Fixed term for 16 months (maternity cover)We will consider flexible working arrangements

Cryo-Electron Microscopy Scientist/Senior Cryo-Electron Microscopy ScientistAstbury Centre for Structural Molecular BiologySchool of Molecular and Cellular BiologyAre you looking for a role to develop your cryo-electron microscopy experiencewithin the context of a world-class electron microscopy facility? Do you have apassion for cutting edge biological and biomedical discovery?We are looking for a structural biologist who is looking to develop and disseminatetheir skills and knowledge in cryoEM working as a cryoEM scientist or senior cryoEMscientist.You will be working within the Astbury Biostructure laboratory, with state-of-the-artequipment including two recently upgraded Titan Krios microscopes with Falcon4/selectris and fringe free illumination. Working as part of the facility team, you willwork on the development and provision of high-end cryo-electron microscopy for ouruser community. Day-to-day you will be involved with the operation of Titan Kriosmicroscopes supporting our community to prepare and image frozen-hydratedspecimens. You will provide guidance, training and advice in areas covering samplepreparation, optimisation, freezing and data acquisition, as well as analysis andinterpretation of EM outcomes. You will contribute your experience to our researchprogramme and running of a cryo-EM facility, where you will have opportunities useboth single particle and tomography methods.You should have a Ph.D. in Structural Molecular Biology and a passion for studyingbiological and biomedical processes. You will work closely, interactively andcollaboratively with the facility team as well as the wider user community, and soexcellent communication and interpersonal skills are essential.The University of Leeds and the Faculty of Biological Sciences are committed toproviding equal opportunities for all and offer a range of family friendly policies. TheUniversity is a charter member of Athena SWAN (the national body that promotesgender equality in higher education), and the Faculty of Biological Sciences wasawarded a Silver award in 2020. We are proud to be an inclusive Faculty that valuesall staff, and are happy to consider job share applications and requests for flexible

working arrangements from our employees. Our Athena SWAN webpage providesmore information.What does the role entail?As a Cryo-electron Microscopy Scientist your main duties will include: Assisting with design and execution of cutting edge research programmesusing cryo-electron microscopy (cryo-EM); Working as part of the facility team to support the EM activities of a range ofacademic and industrial researchers, both internally and externally; Assisting with the design, implementation and optimisation of workflows forsingle particle EM and tomography; Preparing and imaging negative stain and cryo-EM specimens on behalf ofother users; Training users in the operation of TEMs and image processing and analysismethods, with a view to obtaining and analysing cryo-EM data of the highestquality; Supporting user sessions on the Titan Krios; Making a contribution to the dissemination of research results, includingpublications and presentations; Interface and collaborate with IT, assisting with the management andimplementation of EM software including appropriate documentation andtraining resources; Contributing to a world-class, collaborative research environment, includingmentoring junior researchers and training students, and maintaining your owncontinuing professional development, particularly keeping up-to-date withtechnology relevant to cryo-EM science.As a Senior cryo-electron Microscopy Scientist your additional duties will include: Bring experience as a facility based scientist; Independently supporting the breadth of imaging activities in the EM facility,assisting sessions at all levels, including the implementation of novelworkflows; Manage relationships with external partners; Playing a crucial role in trouble shooting, and working with service engineersto bring about resolutions;

Establish and maintain research networks with key staff with the School,Faculty, University and beyond. To maintain an independent network ofcontacts to enhance the internationally excellent reputation of ABSL.Designing and executing cutting edge research programmes using cryoelectron microscopy (cryo-EM) in the Astbury Biostructure Laboratory;Lead the design of training, and implementation of new training resourcesThese duties provide a framework for the role and should not be regarded as adefinitive list. Other reasonable duties may be required consistent with the grade ofthe post.What will you bring to the role?As a Cryo-electron Microscopy Scientist you will have: A PhD (or close to completion), or equivalent experience, in StructuralMolecular Biology; Experience in the operation of electron microscopes; Experience preparing biological specimens for imaging in electronmicroscopes; Experience in data processing of structural biology data, including experiencewith high performance computing and familiarity with UNIX systems; Good organisational, planning and self-management skills, with the ability tosupport a range of different work streams/projects simultaneously and retain aclear focus on outcomes/deadlines; The ability to work effectively as a member of a team; Excellent interpersonal and communications skills with the ability tocorrespond and communicate with a wide range of people at all levels ofexperience and seniority; Experience in teaching in a one-on-one or small group setting.You may also have: Experience with the operation of cryoTEM; Experience of imaging and analysing single particle cryo-electron microscopydata; Experience of imaging and analysing cryo-electron tomography specimens toproduce data;

Experience collecting and processing negatively-stained specimens forelectron microscopy;Experience of the analysis of cryo-EM data to produce 3D structures, includingexperience with a range of different image processing packages;Experience with software for map visualisation and analysis, including modelbuilding and flexible fitting software.As a Senior cryo-electron Microscopy Scientist will have: A minimum of three years post-doctoral experience in cryo-electron microscopyor tomography; Existing experience supporting a multi-user community in their cryoEM studies; Extensive experience in preparing a diverse range of specimens for cryo-EMimaging; Extensive experience of the analysis, visualisation, validation and interpretationof cryo-EM data; Intimate knowledge of the control systems and software of Titan Kriosmicroscopes. This would include the core competence of independently andfully troubleshooting Krios operation for a user of any ability; Experience in data collection for single particle and tomography projects, usingEPU and/or SerialEM. A strong understanding of the computing hardware, software and datarequirements of cryo-EM science, including experience with high performancecomputing and networking; A track record of research outputs in refereed publications of internationallyexcellent quality; Experience managing relationships with external stakeholders such as industrypartners; Outstanding organisational, planning and self-management skills, with theability to support multiple different projects, and fulfil multiple different rolessimultaneously, understand the links between them, and retain a clear focus onoutcomes/deadlines; Ability to contribute to management and administrative processes andstructures, including managing resources and/or staff under challengingcircumstances; Outstanding interpersonal and communications skills with the ability tocorrespond, communicate and shape opinion with a wide range of people at alllevels of experience and seniority;

High motivation to develop and publicise the breadth and quality of the activitiesof Astbury Biostructure Laboratory.As a Senior cryo-electron Microscopy Scientist may also have: Experience in serialEM scripting;Experience scripting EM image processing solutions, which may includethe design and implementation of on-the-fly image;Experience in correlative microscopy;Experience in electron diffraction.How to applyYou can apply for this role online; more guidance can be found on our How to Applyinformation page. Applications should be submitted by 23:59 (UK time) on theadvertised closing date.Your application should include: A supporting statement providing evidence to support each requirement listedon the ‘What will you bring to the role’ section of the Candidate Brief (no morethan two sides of A4, minimum font size 11); An academic curriculum vitae, including a list of your publications.Contact informationTo explore the post further or for any queries you may have, please contact:Rebecca Thompson, Head Research Facilities, Faculty Biological SciencesTel: 44 (0)113 3433384Email: r.f.thompson@leeds.ac.ukNeil Ranson, Professor of Structural Molecular BiologyTel: 44 (0)113 343 7065Email: n.a.ranson@leeds.ac.ukAdditional information

Find out more about the Faculty of Biological Sciences and the School of Molecularand Cellular Biology.Find out more about our Research and associated facilities.Working at LeedsFind out more about the benefits of working at the University and what it is like to liveand work in the Leeds area on our Working at Leeds information page.Candidates with disabilitiesInformation for candidates with disabilities, impairments or health conditions,including requesting alternative formats, can be found on our Accessibilityinformation page or by getting in touch with us at disclosure@leeds.ac.uk.Criminal record informationRehabilitation of Offenders Act 1974A criminal record check is not required for this position. However, all applicants willbe required to declare if they have any ‘unspent’ criminal offences, including thosepending.Any offer of appointment will be in accordance with our Criminal Records policy. Youcan find out more about required checks and declarations in our Criminal Recordsinformation page.

equipment including two recently upgraded Titan Krios microscopes with Falcon 4/selectris and fringe free illumination. Working as part of the facility team, you will work on the development and provision of high-end cryo-electron microscopy for our . and work in the Leeds area on our Working at Leeds information page. Candidates with .