Basics Of Slit Lamp Microscopy - Bhatti Eye

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Basics of slit lamp microscopyDr. S S Bhattiwww.bhattieye.com

The 2 basic parts of the slit lampbiomicroscope are: The slit lamp (illumination system) The biomicroscope

The illumination system can be1. Of the Zeiss type2. Of the Haag Streit type

In the Zeiss type the illuminationcomes from below

In the Haag Streit type theillumination comes from above

In both types of illumination systemthe Kohler illumination principle isused:The filament is imaged on to the objective lens but themechanical slit is imaged on to the patient’s eye

The biomicroscope:based on the optics of a compoundmicroscope Two basic types:– The Grenough type– The Galilean changer type

The Grenough type(Classical HaagStreit)Flip lever to changemagnification

The Galilean Magnification changerKnob to changemagnification (3or 5step)

ObjectiveGalilean magnification changer

Magnification can also be changedby changing the eyepiece power10 X16 X

The slit lamp and the biomicroscopeare maneouvred together on a crossslide by means of a joystick

The coupling between the slit lampand the biomicroscope This is such as to make the system“parfocal” i.e the focus of the slit and the focus of themicroscope are at the same point. This parfocality may occasionally need tobe dissociated as for example in thetechnique of sclerotic scatter

The coupling between the slit lampand the biomicroscope This allows both theslit and themicroscope to rotateabout the point offocus (i.e the eye)

Dissociation of parfocality can be done in“Haag Streit” type slit lamps by looseningthe sclerotic scatter knob

This dissociation of parfocality isuseful for indirect illumination,sclerotic scatter and retroillumination

The key to successfulexamination of theanterior segment isknowledge of thevarious methods oflighting which can beachieved by the slitlamp.

Diffuse illumination Not all slit lamps havethis option

Diffuse illumination for surfacedetails

Diffuse illumination

Diffuse illumination

Diffuse illumination

Focal broadbeam illumination

Broad beamNarrow beam

Focal broad beamBusacca’s noduleon iris

Knob to widen beam

Focal slit illumination

Focal slit illumination

Retroillumination- against red glow

Retroillumination- YAG pits on clawIOL

Indirect illumination(similar tosclerotic scatter)

Sclerotic scatter

Knob for sclerotic scatter allows slitbeam to be horizontally rocked

Parfocality of slit and viewingaltered for sclerotic scatter

Sclerotic scatter

Specular illumination

Filter turret

With additional dyes

CollageBroad beamRetroilluminationagainst red glowslitRetroilluminationagainst lens

Lens precipitatesDiffuseilluminationFocalillumination

SPKS- a collage

Krukenberg spindle

Traumatic rosette cataract

Anatomy of the angle

Normally the angle of the anterior chambercannot be seen as light from it cannot exitfrom the eye due to total internal reflection atthe cornea

A gonioscopy lens allows light fromthe angle to exit the eye byeliminating the cornea air interfaceGoldman gonioscopy lens

Direct GonioscopyKoeppeGonioscopyKoeppegonioscopy lenslens

Angle recession

Trabecular pigmentation

Fundus examinationcan be done with aslit lamp with the useof ancillary lenses Ancillary lenses arerequired to neutralizethe refractive powerof the cornea .

Use of the short reflex mirror is recommended forposterior segment examination because the upwardprojection of the long mirror blocks one of the eyepieces when the illumination is kept at a small 3-5degree angle from the binocularHowever, the illumination beamcolumn must then be tilted elsethe illumination beam will fallpartly outside the mirror reducingthe illumination entering the eye

Broad beamNarrow beam

Some contact Fundus slitlamplensesMainsterHighMagnificationMainster standardMainster PRP(widefield forpanretinalphotocoagulation)

Fundus view with slitlamp andMainster contact lens

Slit lamp examination with a Volkquadraspheric contact lens(dislocated crystalline lens)

Use of the short reflex mirror is recommended for posterior segment examination because the upward projection of the long mirror blocks one of the eye pieces when the illumination is kept at a small 3-5 degree angle from the binocular However, the illumination beam column must then be tilted else the illumination beam will fall