Transcription
crystallographersfavouriteliquid handlers
mosquito for proteincrystallographyThe automation of protein crystallography screening has made asignificant contribution to the rapid expansion of crystallographybased structural biology. Automated liquid handlers such asmosquito crystal and mosquito LCP improve the accuracy,reproducibility and throughput of protein crystallography screens,reducing labour intensive plate preparation for screening,optimisation and scale up as well as eliminating manual pipettingerrors. All our mosquito systems offer:n range of low volumes - robust performance from microlitresto nanolitresn accurate and precise - using positive‑displacementpipetting technology in the 25 nL – 1,200 nL volume rangen cost savings from optimised sample use and reduced wasten zero cross contamination - using disposable pipette tipsn minimal dead volumes - optimising reagent usen more than just dispensing - our instruments are truepipettors, able to aspirate multiple components, dispense andeven mix without splashing or split dropsn ease-of-use - intuitive set-up and software proven inmultiple-user labs; just walk up and use itn fast - no slow wash steps or system fluidsmosquito crystalmosquito LCPprotein crystallisation screeningautomating LCP set-upmosquito crystal is the protein crystallographer’s favourite liquidhandler. It makes protein crystallography screening faster, morecost-effective and quite simply easier than ever before. It bringstogether speed, accuracy and high precision pipetting of nanolitrevolumes with zero cross-contamination from a disposable tip.mosquito LCP is the ultimate tool for membrane proteincrystallisation with all the functionality of mosquito crystal.It allows you to fully automate LCP set-ups accurately andrepeatedly. mosquito LCP allows you to dispense lipidiccubic phase (LCP) volumes as low as 25 nL, while automatedcalibration of syringe and pipette positioning ensures precisedrop-on-drop placement to facilitate automated imaging.n flexible automation – rapid automated plate set-up for allstandard crystallisation techniques: sitting drop, hanging drop,microbatch, bicelles, microseeding and additive screeningn reproducibility - unrivalled reproducibility down to 25 nLn no configuration changes for different experiments- eliminates the need for instrument configuration changeswhen changing techniques or liquid viscositiesn multiple aspiration before a single dispense andmicroseeding, which is essential for automating additivescreeningn unrivaled drop precision - perfectly positioned drops fordownstream imaging by placing protein and screen dropswith the same headn robust day-to-day performance, even in heavy use.Proven reliability with no blocking or cloggingn automation of membrane protein crystallisationscreening, optimisation and scale-up using techniquessuch as LCP and bicellesn versatility - ability to set up both LCP and all traditionalprotein crystallisation experiments with just one instrumentusing commercially available SBS-format platesn flexibility of sample type - precise pipetting acrossa wide range of liquid viscosities with no format changerequiredn speed of set up – rapid tip changing and no washingn active humidity control – mosquito’s active humiditychamber reduces experimental inconsistencies caused byvariation in the humidity in the environment
dragonfly crystalaccessoriesscreen optimisation optimisedLCP mixerdragonfly crystal enhances protein crystal screen optimisation.dragonfly crystal is the ideal system to complement SPTLabtech’s mosquito in the protein crystallisation workflow. Oncethe initial crystal ‘hits’ are identified, dragonfly crystal produces aset of optimised conditions to grow better diffracting crystals.The LCP mixer has beendesigned to automatically mixprotein and monooelin (MO),or other lipids, into a lipidiccubic phase (LCP) from twocoupled syringes. n no liquid classification - making set-up quick and easyn flexible automation - dispense any volume from 0.5 μLupwards to 4 mL, into any well, from any syringe. Independentcontrol of each channeln fast - rapid completion of plate irrespective of viscosityvariation in stocks (5-8 mins)n negligible evaporation of the dispensed reagents due tominimal set-up timen stock integrity - positive displacement technologypreserves stock integrity, even for volatilesn free unlimited screen designer software - interactive,highly intuitive, powerful and simple set-up on multipleinstruments anywhere, anytimen a ccepts any combinationof 100 μL and 50 μLHamilton syringesn a ccepts any samplevolumen s imple and robust designn g love friendly touch screendisplayn o n-the-fly adjustment ofmix cyclesn c ompact unit to savebench spacen n o adjustment orcalibration requiredconsumablesactive humiditychambermosquito’s active humiditychamber reducesexperimental inconsistenciescaused by variation in thehumidity in the environment,by allowing users to accuratelycontrol the relative humidity(RH) of each experiment.n e nables up to a 90%reduction in dropevaporationn i t takes only a few minutesto reach high levels ofhumidity (80-90% RH)n p recisely controlled fromwithin mosquito software(version permitting)n a llows ‘per protocol’control to suit multi-userlabsMXone in-well mixerMXone is the perfectcompanion to dragonflycrystal to mix any liquid insmall wells. The MXonekeeps the plate stationary,allowing for extremely fastmixing of even the mostviscous solutions with no riskof spillage using high-speedoscillation of a disposable pin.n f ast and efficientn z ero cross-contaminationn r ange of viscosities mixedin one platen a void spillage as seen withorbital shakersSPT Labtech consumables aredeveloped and manufacturedto the same high standard ofdesign and engineering as ourinstruments – quality we alsodemand from our suppliers.The combination of cuttingedge innovation and precisionmanufacturing ensuresaccurate results in the lab,every time.
positivedisplacementpipetting:As a protein crystallographer you want to be safe in the knowledgethat the wide range of viscosities regularly encountered is handledand dispensed accurately and repeatably every time without liquidclassification.true positive displacement pipetting of anoptimsation gradient with dragonfly - how it worksThe difficulties of pipetting accurately at low volumes are well known,as are issues associated with handling viscous liquids. Our innovativetechnology development team have overcome these with the noveluse of positive displacement dispensing from disposable tips in allof our liquid handling products. Positive displacement pipetting isdefined by the fact that the piston moving the liquid in and out of thetip for aspiration and dispensing comes into direct contact with theliquid that is being moved. This piston based pipetting action resultsin highly accurate liquid handling across a very broad volume andviscosity range.aspirationpiston rodpositive displacement pipettingpipette barreltheaccurateway to terfacedragonfly uses much larger (4 mL) positive displacement,disposable tips to aspirate from reservoirs and dispense non-contactto wells within plates. It is therefore ideal for combination and bulkreagent dispensing as well as accurate handling of difficult liquidclasses.mosquito uses positive displacement, disposable micropipettes tohandle low volumes (25 nL - 5 uL) of liquid in plate to plate, or intraplate, transfers using contact dispensing - thus it’s ideal for traditionalpipetting tasks that users would like to miniaturise.dispensingpistonlow wellssimpleproductivereliableIntuitive and logical operationNo air gaps or system liquids meanDisposable tips and a piston basedsimplifies user experience andaccurate displacement of liquids,pipetting mechanism with noreduces set up time and trainingirrespective of liquid classpressure system or valves results inrequirementsrobust and reliable performanceversatileeconomicalinnovativeMore than just dispensing - ourDirect sample aspiration by theMotor driven pistons reliably ejectinstruments are true pipettors, abletip ensures minimal sample deadliquid from the tips directly, providingto aspirate, dispense and even mixvolumesextremely repeatable dispensing
mosquito crystalspecificationsmosquito LCPdragonfly crystalpipettingrange25 nL – 1.2 μL25 nL – 1.2 μL0.5 μL – 4 mLapplicationsproteincrystallographyset-ups e.g.additive screening,microseeding,microbatch, bicelleslipidic cubic phase(LCP) screening plusall the functionalityof mosquito crystalprotein crystaloptimisationand assaydevelopmentwithoutcontamination orliquid classificationprimary SBSplate format48, 96, 38448, 96, 38415, 24, 48, 96,(384)dead volume 0.3 μL 0.3 μL 0.5 mLoptionalextrasactive humiditychamberactive humiditychamber, LCP mixerMXone automatedin‑well mixerthroughput 2 mins/ 96-wellplate,4 mins/ 288 drops2 mins/ 96 dropplate for vapourdiffusion 5 mins/ 96drop LCP plate4–6 ingredient,96-well plate in 4–8mins, irrespective ofviscosityget in touchSPT Labtech LtdSPT Labtech IncSPT Labtech India Private Ltd 44 (0) 1223 627 555discover@sptlabtech.com 1 (855) 601 5867discover@sptlabtech.com 91 9968291292enquiries@sptlabtech.comfind us onsptlabtech.com
SPT Labtech Ltd 44 (0) 1223 627 555 discover@sptlabtech.com SPT Labtech Inc 1 (855) 601 5867 discover@sptlabtech.com SPT Labtech India Private Ltd 91 9968291292 enquiries@sptlabtech.com get in touch find us on sptlabtech.com
