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BD Biosciences Instrument and Application TrainingsTo help customers take full advantage of the capabilities of BD instruments quickly and efficiently, BDBiosciences offers a variety of training options for instruments and applications. Professional BD instructors haveextensive flow cytometry experience and have worked in research and clinical labs. Courses combine theory withhands-on practice to provide participants with the skills and experience to take full advantage of BD Biosciencesproducts and stay updated with the latest technology.Our trainings are mostly conducted in the Stockholm training lab. For courses in our Stockholm training lab, weprovide you with coffee and break snacks, as well as lunch.TMTMTMBD FACSAria , BD FACSJazz and BD Influx trainings are held at the European Training Center in Belgium.We provide you with coffee, drinks and break snacks as well as lunch. The price also includes hotelaccommodation in Ghent or Aalst, as well as the transport to/from the airport and hotel.For more info and quotes, please contact us at bdbnordic@europe.bd.com.For more information please click on the instrument or application course of interest.BD Instrument trainings:BD FACSCaliburBD FACSCantoBD FACSVerseTMTMIITMBD LSRFortessaBD FACSTMTMSample Prep Assistant IIIBasic BD FACSAriaTM Systems Operator CourseAdvanced BD FACSAriaTM Systems Operator CourseBD FACSJazz BD InfluxTMBD Application trainings:BD Multicolor Flow Cytometry TrainingAnalysis of Phosphorylated Proteins at Single Cell LevelCancellation Policy
BD FACSCaliburTM operator trainingCourse ObjectivesTMThis course is for operators of the BD FACSCalibur Systems.TMAfter completing the BD FACSCalibur course, participants will: understand the basic concepts of flow cytometry be familiar with components and functions of the cellanalyzer and the software know how to operate and maintain the instrument be able to perform acquisition and analysis independently.Course contentThis operator course is a practical- oriented training course that combines general and instrument specificbackground information with hands-on sessions.Course prerequisitesWe recommend that participants have completed the Introduction to flow cytometry, a self-paced e-learningcourse that takes you through the general concepts and principles behind flow cytometry. Duration 1 /self paced.jspDuration2 daysLocationBD Training lab in StockholmBD FACSCantoTM II operator courseCourse ObjectivesTMThis course is for operators of the BD FACSCanto II flow cytometer.TMAfter completing the BD FACSCantoII course, participants will: understand the basic concepts of flow cytometry be familiar with components and functions of the cell analyzerand the software know how to operate and maintain the instrument be able to perform acquisition and analysis independently.Course contentThis operator course is a practical- oriented training course that combines general and instrument specificbackground information with hands-on sessions.Course prerequisitesWe recommend that participants have completed the Introduction to flow cytometry, a self-paced e-learningcourse that takes you through the general concepts and principles behind flow cytometry. Duration 1 /self paced.jspDuration3 daysLocationBD Training lab in Stockholm
BD FACSVerseTM operator courseCourse ObjectivesPractical sessions cover: Standard operation and maintenance procedures Quality control (CS&T) Multicolor data acquisition and analysis When applicable, BD FACS Universal Loader option operation Using the Worklist function in manual or loader mode BD FACSuite software Tips for troubleshooting, sample preparation and handling Data managementTheoretical presentations include: Basic concepts of flow cytometry Main parts and operational modes Overview on fluorescent dyes, spectral spill over & compensationCourse prerequisitesParticipants ideally have some background in flow cytometry, fluorescence theory and compensation.Course contentThis operator course is a practical- oriented training course that combines general and instrument specificbackground information with hands-on sessions.Duration3 daysLocationBD Training lab in StockholmBD LSRFortessaTM operator courseCourse ObjectivesTMThis course is for operators of the BD LSRFortessaTMAfter completing the BD LSRFortessa course, participants will: understand the basic concepts of flow cytometry be familiar with components and functions of the cellanalyzer and the software know how to operate and maintain the instrument be able to perform acquisition and analysisindependently.Course contentThis operator course is a practical-oriented training course that combines general and instrument specificbackground information with hands-on sessions.Course prerequisitesWe recommend that participants have completed the Introduction to flow cytometry, a self-paced e-learningcourse that takes you through the general concepts and principles behind flow cytometry. Duration 1 /self paced.jspDuration3 days basic training
LocationIn houseBD FACSTM Sample Prep Assistant III (SPA III) operator trainingCourse ObjectivesTMAfter completing the BD FACS SPAIII course, participants will: be familiar with components and functions of the SPAand its software know how to operate and maintain the instrument know how to create and run a worklist on the SPA andhow to import a SPA worklist into BD FACSCantoClinical software and/or BD FACSDiva softwareCourse contentThis operator course is a practical- oriented training course thatcombines general and instrument specific background informationwith hands-on sessions.Course prerequisitesBasic computer skills are required for the courseDuration1 dayLocationBD Training lab in StockholmBasic BD FACSAria systems Operator CourseCourse ObjectivesAfter completing the operator course, participants will: understand the basic concepts of flow cytometry andcell sorting be familiar with components and functions of the cellsorter and BD FACSDiva software know how to operate and maintain the instrument be able to perform 2-way, 4-way and single cell sort aswell as multicolor data acquisition and proper gatingstrategies.Course contentThis operator Course is a practical training course that combines general and instrument specific backgroundinformation with hands-on sessions.For operators without previous knowledge of flow cytometry, a pre-training of one day can be arranged at theTMcustomer site as a preparation for the BD FACSAria II/III basic operator training. Please request via email atbdbnordic@europe.bd.comCourse prerequisitesWe recommend that participants have completed the Introduction to flow cytometry, a self-paced e-learningcourse that takes you through the general concepts and principles behind flow cytometry. Duration 1 /self paced.jsp
Duration4 days from Monday noon until Friday noonLocationBD European Training Center, Erembodegem, BelgiumAdvanced BD FACSAria systems Operator CourseCourse ObjectivesAfter completing the operator course, participants will be able to: operate, maintain and troubleshoot the instrument setup the instrument by selecting relevant controls forperformance, compensation and gating design multicolor experiments up to 10 colors adapt default sorting settings to optimize speed,viability, purity and efficiency perform optimized sorts on different applicationsincluding various samples and rare cell populationsCourse contentThis operator Course is a practical training course that combines general and instrument specific backgroundinformation with hands-on sessions.Course prerequisitesParticipants must have good knowledge of BD FACSDivaTM software, including general acquisition and analysisfunctions, automated compensation and basic CS&T tasks. Knowledge of the concepts of flow cytometry as wellas multicolor applications is required.Duration4 days from Monday noon until Friday noonLocationBD European Training Center, Erembodegem, BelgiumBD JazzTM Operator CourseCourse ObjectivesAfter completing the BD FACSJazz Operator Course, you will: under stand the basic concepts of flow cytometry andcell sorting be familiar with the components and functions of BDFACSJazz and the BD FACS Sortware software be able to do multi-colour data acquisition and propergating strategies using the BD FACS Sortware software know how to operate and maintain the instrument be able to perform a 2-way sort into tubes as well assorts of plates including single-cell sorting and indexsortingCourse contentPractical sessions cover: Standard maintenance procedures Stream and laser alignment Reset of nozzle after cleaning and exchange of fluidics lines
Standard instrument operation including 2-way sort, plate sorting and index sortingGeneration of customized operation - and QCworksheetsDate management and data acquisition using the BD FACS Sortware softwareMulticolor gating and compensationTheoretical presentations include: Basic concepts of flow cytometry and cell sorting Main parts and operational modes Instrument specific optics, fluidics and electronics Overview on fluorescent dyes & compensation Tips for troubleshooting, sample-preparation andCourse prerequisitesParticipants ideally have some background in flow cytometry, fluorescence theory and compensation. Werecommend that participants have completed the Introduction to flow cytometry, a self-paced e-learning coursethat takes you through the general concepts and principles behind flow cytometry. Duration 1 /self paced.jspDuration4.5 daysLocationBD European Training Center, Erembodegem, BelgiumBD InfluxTM Operator CourseCourse ObjectivesTMAfter completing the BD Influx operator course, participants will: understand the basic concepts of flow cytometry and cellsorting be familiar with components and functions of the cellsorter and its software know how to operate and maintain the instrument be able to perform 2-way, 4-way and single cell sort aswell as multicolor data acquisition and proper gatingstrategies.Course contentThis operator Course is a practical- oriented training course that combines general and instrument specificbackground information with hands-on sessions. Participants can expect effective training classes that focus onindividual hands-on sessions in small groups (max. 2 persons per instrument).Practical sessions cover: Standard operation and maintenance procedures Stream and laser alignment Change of nozzles and fluidics lines Multicolor data acquisition and sorting strategies Data collection, gating and compensation with FACS SORTware software Tips for troubleshooting, sample preparation and handlingTheoretical presentations include: Basic concepts of flow cytometry and cell sorting Main parts and operational modes Instrument specific optics, fluidics and electronics Overview on fluorescent dyes, spectral spillover & compensation
Course prerequisitesParticipants ideally have some background in flow cytometry, fluorescence theory and compensation. Werecommend that participants have completed the Introduction to flow cytometry, a self-paced e-learning coursethat takes you through the general concepts and principles behind flow cytometry. Duration 1 /self paced.jspDuration4.5 daysLocationBD European Training Center, Erembodegem, BelgiumBD Multicolor Flow Cytometry: Optimized Solutions for MulticolorExperiments by flow CytometryCourse ObjectivesAfter completing the BD flow cytometry training, participants will: understand the principles of multicolor panel designand how to optimize cytometer settings to yourapplications be able to design and run multicolor experiments on aBD digital flow cytometer.Course contentThis training is practical-oriented and combines general and software specific background information with handson sessions.Practical sessions cover: Set up and Quality Control of BD Flow Cytometers “Fluorogame” to memorize the different fluorochrome characteristics Compensation Multicolor Panel design exercisesData recording and analysis of eight-color experimentsTheoretical presentations include: Set up and Quality Control of BD Flow Cytometers Characteristics and combination of fluorochromes Prevention of artefacts in Muticolor Flow cytometry: Compensation related artefacts Multicolor experiments for intra-cellular staining Negative controlsMulticolor panel designCourse prerequisitesCompletion of a BD operator course for a digital cytometer using BD FACSDiva software v6 or equivalentexperience on a BD digital cytometer.Duration2.5 daysLocationBD Training lab in Stockholm
BD Phosflow Training: Analysis of Phosphorylated Proteins at SingleCell LevelCourse ObjectivesAfter completing this course you will be able to: Set up your own multicolour experiment for detecting intracellularphosphorylated proteins in different subpopulation of cells. Optimize your Phosflow experiment in the choice of fluorochromesand protocols. Optimize instrument setup for cells that have been treated withBD Phosflow protocol. Use web-based tools and databases to analyse and share FACS data.Course contentThis training is practical-oriented and combines general and software specific background information with handson sessions.Practical sessions cover: Instrument Quality control (QC) using CS&T Set up instrument for optimal detection of Phosflow Acquisition and analysisSample preparation & optimizationTheoretical presentations include: Set up and Quality Control of BD Flow Cytometers for optimized multicolor experiments Compensation theory for multiparameter analysis Optimizing choice of antibody/fluorochrome when performing staining of both surface markers andintracellular proteins. Cytobank, an analysis tool Set up your own Phosflow experimentDiscussion and of resultsCourse prerequisitesBasic knowledge in: Flow Cytometry Cell Biology and Cell signalling Diva software or FACSSuiteDuration2 daysLocationBD Training lab in Stockholm
Cancellation PolicyWe accept cancellations in written form to the following email address: bdbnordic@europe.bd.comBy customer :- Cancellations received at least 21days prior to the class start date can be communicated to yourlocal coordinator.- If a cancellation or reschedule request is received fewer than 21 days before the start date, acancellation fee may be applied.- Attendance by a replacement person is free of charge.By BD:- BD Biosciences reserves the right to cancel a training class due to low demand, unforeseenhigh number of cancellations or other circumstances making it impractical to hold the course.- If a course is cancelled BD will provide an alternative date for the course.BD Biosciences Nordic Application Team:Paul RaymondTraining and Applications Support Manager, North West EuropeE-mail: Paul Raymond@europe.bd.comNoora AllonenNordic Application Specialist / FinlandE-mail: Noora Allonen@europe.bd.comSebastian HedlundNordic Application Specialist / SwedenE-mail: Sebastian Hedlund@europe.bd.comMorten LøbnerNordic Application Specialist / DenmarkE-mail: Morten Lobner@europe.bd.comLinda SapulaNordic Application Specialist / SwedenE-mail: Linda Sapula@europe.bd.comLaura SilvennoinenBDB Nordic Training CoordinatorE-mail: Laura Silvennoinen@europe.bd.com
BD European Training Center, Erembodegem, Belgium Advanced BD FACSAria systems Operator Course Course Objectives After completing the operator course, participants will be able to: operate, maintain and troubleshoot the instrument setup the instrument by selecting relevant controls for performance, compensation and gating
