WIXLIB

IMB X-Ray Facility OverviewInstitute of Molecular BiophysicsX-Ray Facility (XRF)410-413 Kasha Laboratorywww.sb.fsu.edu/ xray850-644-6448 2002-11 ThayumanasamySomasundaram1

IMB MOB XRF IMB interdisciplinaryInstituteHome to MOB PrgmChemistry, Biology,Physics, Mathematics,and EngineeringMajor research focus: Structural BiologyComputationalBiophysics X-Ray Facility wasestablished in 1993One of the four CoreFacilities in IMBOpen Access to FSUand SUS InstitutionsNominal fees for usageSafety & On-siteTraining Required 2002-11 ThayumanasamySomasundaram2

What XRF Can Do For You?Images courtesy of Michael Chapman LabDataCollectionDiffractionCrystalXRF canhelp youin Stepse- DensityAtomicModeling 2002-11 ThayumanasamySomasundaramStructure3

Service and ResourcesData CollectionAmenitiesX-Ray Generator & MirrorSystem Digital Detector & ControlData Processing Extract intensity and index Obtain phase information Refine and fit the data tomodel Crystal Room Cold Room Digital Imaging Synchrotron TimeResources Screening & Crystallization Data Collection Strategy Crystal Storage &Transport Synchrotron Time & Usage 2002-11 ThayumanasamySomasundaram4

X-Ray Generator & Mirror Rigaku RU-H2R Cu Rotating Anode Brilliant Beam Osmic Confocal Mirror Wavelength Purity High Flux 2002-11 ThayumanasamySomasundaramwww.rigaku.com5

X-Ray Detectors Imaging PlateRigaku R-Axis IIcTwo 19cm2 IPs8 minute Read-outCCDMarCCD165Single CCD3 s Read-out 2002-11 ThayumanasamySomasundaram6

Computer and SoftwareLinux and/or Windows O/S Software for machine control andcollection Software for extracting intensityinformation Data storage and retrieval 2002-11 ThayumanasamySomasundaram7

X-Ray (Cryo) Data Collection MarCCD/CrystalClear GUI Automated Analog»Digital Oxford CryoSystem Collect at 100 K Protects Crystals Sharper eDensitywww.oxfordcryosystems.co.uk 2002-11 ThayumanasamySomasundaram8

CryoCrystallography Flash Cool in Stream For ImmediateCollection Ease of HandlingFlash Cool in Liquid For Storage For Shipping &TransportFlash Cool in StreamFlash Cool in Liquid 2002-11 ThayumanasamySomasundaram9

How to become a user?Contact the Director of IMB Preliminary trial (free) Bring protein for crystallization screen or Bring crystal for diffraction Establish an account with XRF Independent or collaborative work 2002-11 ThayumanasamySomasundaram10

XRF-ContactThayumanasamy “Soma”sundaram414 Institute of Molecular BiophysicsFlorida State UniversityTallahassee, FL 32306-4380Phone: 850-644-6448 Fax: 850-644-7244E-mail: tsomasundaram@fsu.eduWeb: www.sb.fsu.edu/ xray 2002-11 ThayumanasamySomasundaram11

Chemistry, Biology, Physics, Mathematics, and Engineering Major research focus: Structural Biology Computational Biophysics X-Ray Facility was established in 1993 One of the four Core Facilities in IMB Open Access to FSU and SUS Institutions Nominal fees for usage Safety & On-site Training Required