WIXLIB

OrganicGPC/SEC columnsProduct guide

ContentsAgilent PLgel GPC Columns  3Specialist Columns  PLgel MIXED  5Agilent EnviroPrep  18PLgel 20 µm MIXED-A  6PL HFIPgel  19PLgel 10 µm MIXED-B  7PLgel 5 µm MIXED-C  818Agilent PL Rapide  20Agilent PlusPore  21PLgel 5 µm MIXED-D  9PLgel 3 µm MIXED-E  10Agilent PolyPore  22PLgel MIXED-LS  11Agilent ResiPore  23PLgel MiniMIX Narrow Bore  12Agilent MesoPore  24PLgel Individual Pore Size Columns  14Agilent OligoPore  25PLgel Preparative Columns  16PLgel Olexis  17Selection guide  26Over 30 years’ experience in GPC/SEC1976PLgel columns,individual standards andstandard kitsPolymer Laboratoriesfounded to develop marketleading products for organicGPC/SEC1981PLgel MIXED columns,PL aquagel columnsMIXED columns improvedata quality, and novelchemistries for analysis ofwater-soluble polymers1984GPC softwareDedicated softwarestreamlinesGPC/SEC calculations219901993PL aquagel-OH columnsVastly improve resolutionand data quality in aqueousGPCEasiCal standardsNew format shortens samplepreparation time and thespeed of calibration

Agilent PLgel GPC ColumnsFor polymer applications using organicsolventsThe PLgel rangePLgel materials have high pore volume and high efficiency to maximizeresolution. Their unequalled solvent compatibility makes for easy transferbetween polar and non polar eluents, and outstanding physical rigidityprovides extended lifetimes that minimize downtime. PLgel MiniMIX Narrow Bore saves solvent costs PLgel MIXED for polydisperse materials PLgel MIXED LS for light scattering and viscometry applications PLgel Individual Pore Size Columns for specific applications PLgel Preparative Columns for polymer fractionation PLgel Olexis for the analysis of polyolefinsThe key to successful GPC separations is the correct choice of columns.The comprehensive range of PLgel products has been designed to covervirtually all organic solvent-based polymer analysis application areas, andto make selection of the correct column, solvent and calibration standardfast and reliable.Temperature stability to 220 CPLgel columns can be used at temperatures up to 220 C and operatingpressures up to 150 bar (2200 psi).PLgel is a highly cross linked, porous polystyrene/divinylbenzene matrix,which is recognized as a market leader in GPC column technology.Manufactured and packed exclusively by Agilent since 1976, PLgel ismanufactured to ISO 9001:2000 and benefits from comprehensive QC/QAfor total reproducibility, batch to batch and column to column.1999PL-GPC 220 instrumentMarket-leading hightemperature GPC systemfor routine analysis of eventhe most difficult samplesby multi-detector GPC attemperatures up to 220 ºCRobust performance under the most exacting conditionsElevated temperature is used in GPC either to reduce eluent viscosity, forexample in polar solvent applications, or to maintain sample solubility, as inpolyolefin applications.2003200420072009PL-GPC 50 instrumentwith light scattering andviscometryCost-effective solution tolow temperature polymeranalysis, including multidetector GPC/SECPlusPore columns andEasiVial standardsNew chemistries deliverhigh-pore-volume materialsfor increased resolution, andEasiVial standards simplifycalibration procedures evenfurtherPLgel Olexis columnsOptimized for polyolefinanalysis with highestresolution and data qualityfor even ultrahigh molecularweight samples390-MDS Multi DetectorSuite and PolarGelcolumnsThe 390-MDS turns anyLC into a powerful multidetector GPC system, andPolarGel columns analyzepolar samples in any solventsystem3

Solvent compatibilityPLgel frit porosityPLgel columns are routinely supplied in ethyl benzene* but you can easilyand rapidly transfer between solvents of varying polarity. In organicGPC, sample to column interaction may occur occasionally and eluentmodification can be used to eliminate these effects. PLgel columns arethe ideal choice for such analyses, as they easily tolerate eluents in the pHrange 1-14, as well as up to 10% water in a miscible organic solvent.Media typePorosity (μm)PLgel 3 µm2PLgel 5 µm2PLgel 10 µm5PLgel 20 µm10Ordering informationPLgel is compatible with all of these solventsPLgel Column accessoriesSolvent ty (pk)Part No.7.3Hexane1PL1310-00018.2CyclohexaneFrit Removal Tool for ThreadedColumns only8.9Toluene5PL1310-00029.1Ethyl acetateFrit (2 μm) Kit for Threaded Columns,7.5 mm ID9.1Tetrahydrofuran (THF)Frit (5 μm) Kit for Threaded Columns,7.5 mm ID5PL1310-00129.3ChloroformPL1310-0036Methyl ethyl ketone (MEK)Frit (10 μm) Kit for Threaded Columns,7.5 mm ID59.39.7DichloromethanePLgel 10 μm Column Repair Gel1PL1410-01019.8DichloroethenePLgel 5 μm Column Repair obenzene (o-DCB)Column Connecting Nuts, 1/16 in.Tube10.0Trichlorobenzene (TCB)Tubing Ferrules, 1/16 in. Tube5PL1310-000810.2m-CresolConnecting Tubing, 10 cm Length,0.01 in. ID10PL1310-004810.2o-Chlorophenol (o-CP)10.7Pyridine10.8Dimethyl acetamide (DMAc)11.3n-Methyl pyrolidone (NMP)12.0Dimethyl sulfoxide (DMSO)12.1Dimethyl formamide (DMF)See also Polymer Calibration Standards, with highly characterized molecularweights, publication 5990-7996EN* We also provide a custom packing service in which columns can be shipped inspecific solvents to provide extra convenience to our customers.Tip: For good quality GPC make sure your solvent matches thepolarity of your sample and packing material.4

PLgel MIXEDFor polydisperse materials covering a very broad range ofmolecular weightsPLgel MIXED gel calibration curvesMIXED gel calibration curves are designed to be linear over a specifiedmolecular weight range, ensuring that the same degree of resolution isachieved across the full operating range of the column. The particle sizeof the packing and porosity of a particular MIXED gel column are carefullymatched to the MW range and application, thus optimizing performanceand eliminating the effects of shear degradation. Resolution in GPC iscontrolled by the slope of the calibration curve and the particle size ofthe packing material. Agilent has scientifically determined the minimumnumber of MIXED gel columns required to perform accurate MWDdeterminations based on specific resolution (Rsp). Thus you can havecomplete confidence in the accuracy and precision of the calculated data.The PLgel MIXED range greatly simplifies column selection for easydecision making. Using these mixed columns you can eliminatemismatched column sets and spurious peaks for more reliable results.Simply add extra columns for even greater resolution.Analysis of polydisperse materials was traditionally achieved by combiningindividual pore size columns in series to accommodate the molecularweight range of the polymer. However, this approach is often problematicbecause spurious peak shapes could be introduced and precision lost inthe calculated averages due to “mismatched” calibration curves. MIXEDgel columns overcome these drawbacks.The modern approach to column selection for polydisperse materials is tochoose MIXED gel columns from Agilent. Every column contains a mixtureof individual pore size materials, accurately blended to cover a specifiedbroad range of molecular weight with a linear calibration to eliminatecolumn mismatch.Key20 µm MIXED-A10 µm MIXED-B5 µm MIXED-C5 µm MIXED-D3 µm MIXED-EPLgel MIXED column selection guideAs market leaders in this field, our comprehensive range of MIXED gel GPCcolumns are designed for specific application areas.PLgel MIXED gel column selection guideFigure 1. PLgel MIXED gel calibration curvesUHMW polymerdistributionsPLgel 20 µm MIXED-AHigh MW polymers,demanding eluentsPLgel 10 µm MIXED-BMid range MW polymers,high resolutionResins, condensationpolymersLow MW resins,prepolymersReferenceMeehan, E. (1998) Size exclusion chromatography columns from PolymerLaboratories. In: Chi-San Wu (Ed.) Column Handbook for Size ExclusionChromatography. Academic Press, New York, USA.PLgel 5 µm MIXED-CSee also PLgel MiniMIX Columns, reduce the need for expensive solvents,page 12PLgel 5 µm MIXED-DPLgel 3 µmMIXED-E Polymer Calibration Standards, with highly characterized molecularweights, publication 5990-7996EN5

PLgel 20 µm MIXED-AFor polymers containing high MWmaterialTypical applicationsPolyolefins, polybutadienes, starches, polyisoprenes Extremely high exclusion limit tailored to the MW (molecular weight) ofthe applicationConditionsColumns:Eluent:Flow Rate:Temp:Detector: Large particle size matched to the MW range for optimum performance Low shear prevents sample degradation4 x PLgel 20 µm MIXED-A, 300 x 7.5 mmDMSO 5mM NaNO31.0 mL/min80 CPL-GPC 220 (RI)CharacteristicsLinear MW Operating Range: 2000 to 40,00,000 g/mol (PS equiv)Guaranteed Column Efficiency: 18,000 p/mTypical Pressure:1 mL/min (7.5 mm ID): 3 bar (44 psi) per 300 mm0.3 mL/min (4.6 mm ID): 2.4 bar (35 psi) per 250 mm(THF @ 20 C, TCB @ 140 C)Maximum Flow Rate:7.5 mm ID: 1.5 mL/min4.6 mm ID: 0.5 mL/minMaximum Pressure: 150 bar (2175 psi)Figure 2. StarchesMaximum Temperature: 220 CRecommended no. of Columns/set:4 x 250 mm, 4 x 300 mm or 2 x 600 mmConditionsColumns:Sample:Eluent:Flow Rate:Detector:Recommended Calibrants:EasiVial PS-H for convenient 12 point calibration in just three injectionsEasiCal PS-1 or S-H2-10 Kit provides rapid 10 point calibrationS-H-10 plus S-M2-10 Kits for accurate 19 point calibrationPeak Identification1. 7,500,0002. 841,7003. 148,0004. 28,5005. 2,930See publication 5990-7996EN, GPC/SEC Standards Product GuideOrdering informationPLgel 20 μm MIXED-A ColumnsDescriptionPart No.PLgel 20 µm MIXED-A, 300 x 7.5 mmPL1110-6200PLgel 20 µm MiniMIX-A, 250 x 4.6 mmPL1510-5200PLgel 20 µm MIXED-A, 600 x 7.5 mmPL1110-8200PLgel 20 µm Guard, 50 x 7.5 mmPL1110-1220PLgel 20 µm MiniMIX-A Guard, 50 x 4.6 mmPL1510-12004 x PLgel 20 μm MIXED-A, 300 x 7.5 mmEasiCal PS-1THF1.0 mL/minUV, 254 nm6. 2,560,0007. 320,0008. 59,5009. 10,85010. 580Figure 3. Polystyrene standards separationSee also PLgel MiniMIX-A Narrow Bore Columns, reduce the need for expensivesolvents, page 126

PLgel 10 µm MIXED-BHigh temperature applications withaggressive or unusual solventsTypical applicationsPolyolefins, fluoropolymers, acrylics/acrylates, cellulose derivatives Wide MW operating range maximizes column usefulnessConditionsColumns:Eluent:Flow Rate:Temp:Detector: Low operating pressure minimizes wear on the GPC system Wide range of applications simplifies column choice3 x PLgel 10 µm MIXED-B 300 x 7.5 mmo-Chloronaphthalene1.0 mL/min210 CPL-GPC 220 (RI)CharacteristicsLinear MW Operating Range: 500 to 10,000,000 g/mol (PS equiv)Guaranteed Column Efficiency: 35,000 p/mTypical Pressure:1 mL/min (7.5 mm ID): 10 bar (145 psi) per 300 mm0.3 mL/min (4.6 mm ID): 8 bar (116 psi) per 250 mm(THF @ 20 C, TCB @ 140 C)Maximum Flow Rate:7.5 mm ID: 1.5 mL/min4.6 mm ID: 0.5 mL/minMaximum Pressure: 150 bar (2175 psi)Maximum Temperature: 220 CRecommended no. of Columns/set:3 x 250 mm, 3 x 300 mm or 1 to 2 x 600 mmRecommended Calibrants:The EasiVial PS-H for convenient 12 point calibration in just threeinjectionsFigure 4. Polyphenylene sulfidesEasiCal PS-1 or S-H2-10 Kit provides rapid 10 point calibrationPolystyrene S-H-10 plus S-M2-10 Kits for accurate 19 point calibrationConditionsColumns:Eluent:Flow Rate:Temp:Detector:See publication 5990-7996EN, GPC/SEC Standards Product GuideSee also PLgel MiniMIX-B Narrow Bore Columns, reduce the need for expensivesolvents, page 123 x PLgel 10 µm MIXED-B, 300 x 7.5 mmTCB1.0 mL/min160 CRIOrdering informationPLgel 10 μm MIXED-B ColumnsDescriptionPart No.PLgel 10 µm MIXED-B, 300 x 7.5 mmPL1110-6100PLgel 10 µm MiniMIX-B, 250 x 4.6 mmPL1510-5100PLgel 10 µm MIXED-B, 600 x 7.5 mmPL1110-8100PLgel 10 µm Guard, 50 x 7.5 mmPL1110-1120PLgel 10 µm MiniMIX-B Guard, 50 x 4.6 mmPL1510-1100Figure 5. Polyethylenes7