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HPLC ColumnSelection GuideQuality perfected for the intended useNot for distribution, to be used onlyon Merck/MilliporeSigma devicesThe life science business of Merck operatesas MilliporeSigma in the U.S. and Canada.MK PG3274EN
Would you likeInformation oncolumns for QC?Are you working withLarge Moleculesor Biopolymers?Are you workingSmallMolecules?withExplore the Ascentis Express Range(U)HPLC Performance and Reliability
xWould you likeInformation oncolumns for QC?Searching for SizeExclusion (SEC) or IonExchange (IEX) options?Please contactyour specialistAre you working withLarge Moleculesor Biopolymers?OurRecommendationis theBIOshell Range(U)HPLC Performance and ReliabilityAre you workingSmallMolecules?withExplore the Ascentis Express RangeFor the Porous Particlerange (which can be scaledup for production)our Recommendation isDiscovery Range
UHPLC/HPLC Analysis ofSmall MoleculesAre you analyzingNon PolarCompounds?HPLCC18Are you analyzingModerately Polar Compounds?Are you analyzingVery Polar Compounds?Is your retention too short using C18,or do you need different selectivity?Not Enough Retentionusing C18 (LC-MS)Are yourcompoundsPhenolic, Acidicor Hydroxylated?Are yourcompoundsAromatics?Do you haveBasicCompounds?HILIC Modeusing Silica?New MethodDevelopmentHILIC Mode?HPLCRP-AmideHPLCPhenyl-HexylHPLCF5 (PFP)HPLCHILIC (Si)HPLCZIC-HILICSeQuant Ascentis Express HPLC 2.7 and 5 µm (pressure up to 600 bar)UHPLCC18UHPLCRP-AmideUHPLCPhenyl-HexylUHPLCF5 (PFP)UHPLCHILIC (Si)Ascentis Express UHPLC 2.0 µm (pressure up to 1000 bar)(U)HPLC Performance and ReliabilityBack to OverviewDo you havevery matrix-richsamples?Chromolith
UHPLC/HPLC Analysis ofLarge Molecules (Proteins, Peptides or Glycans)IgGs& Proteins 100 kDaProteins2 kDa MW 500 kDaHydrophilicProteinsBIOshell IgG 1000 ÅC4Medium PolarProteinsBIOshell 400 ÅProtein C18PeptidesMW 15-20 kDaGlycans 2-3 kDaHydrophobicProteinsPeptides &PolypeptidesPolar orMembranePeptidesReleasedGlycansBIOshell 400 ÅProtein C4BIOshell 160 ÅPeptide C18BIOshell 160 ÅPeptide CNBIOshell 90 ÅGlycanCustomizedImmobilizationSuperficially Porous Particles (SPP)Chromolith WP 300RP-C41Chromolith WP 300RP-18Chromolith WP 300RP-8Chromolith WP 300RP-4Chromolith HR RP-18e(150 Å)Chromolith WP 300Epoxy3Chromolith WP 300Protein A2Very Matrix Rich Samples123The Chromolith WP 300 C4 consists of a macropore volume of 2 µm (20.000 Å) enabling the separation of very large moleculesAffinity chromatography for specific binding of IgGsFor customized immobilization on demand(U)HPLC Performance and ReliabilityBack to Overview
UHPLC/HPLC Analysis ofLarge Molecules using fully porous particles (FPP)Proteins2 kDa MW 500 kDaHydrophilicProteinsMedium PolarProteinsHydrophobicProteinsDiscovery BIO C18Discovery BIO C8Discovery BIO C5For Use in Scale-Up Applications(U)HPLC Performance and ReliabilityBack to Overview
QC Solutions for small and large moleculesContact your specialist for more detailsAscentis Express (90 Å)BIOshell (160, 400, 1000 Å)Purospher STAR (120 Å) Outstanding peak symmetry Stable from pH 1.5 - 10.5 Perfect reproducibility(Masterbatch) 100% aqueous phase compatibleSeQuant (100, 200 Å) Choice of three phases withzwitterionic functionalitydesigned for HILIC mode Suitable for LC-MS useAscentis / Discovery Wide range of phasesDiscovery BIO (300, 1000 Å) Three choices for reverse-phasemode and two choices for ion exchange mode(U)HPLC Performance and ReliabilityValidation Kitswith 3 differentbatchesavailable Outstanding column efficiency Fast, efficient separationson HPLC and UHPLC Wide range of phases Available in micro formatChromolith (150 Å)Chromolith WP (300 Å) Rugged and robust technologyAnalysis of matrix-rich samplesLow backpressureIncreased column lifetimeAvailable in micro formatBack to Overview
HPLC Column Selection Guide Quality perfected for the intended use. Would you like Information on columns for QC? Are you working with Large Molecules or Biopolymers? Are you working with Small Molecules? Explore the Ascentis Express Range (U)HPLC Performance and Reliability. For the Porous Particle range (which can be scaled up for production) our Recommendation is Discovery Range Are
