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CyFlow MLHealthcare Immunology PathologyMicrobiology Cell BiologyMultilaser 16 Parameter Desktop FCM System

01COMPANYFlow Cytometry made in GermanyNew sophisticated applications and increasing requirementsfor reliable results in research and routine within shortestpossible time - The challenge for flow cytometry instrumentation, automation and software.A well-established network of subsidiariesand distributors in more than 60 countriesworldwide characterizes Partec’s commitment to the increasing focus and needfor global access to Flow Cytometryinstrumentation and application support:www.partec.de/partec/distributors.htmlMore than 35 Years of Experienceand Professional ExpertisePartec - pioneer in Flow Cytometry since 1968 - respondsto these requirements with the new generation ofWindows XP based CyFlow and PAS FCM systems featuring innovative computer controlled flow systems,modular optical systems with advanced PMTs for all opticalchannels, recently available latest computer and electronic technologies including fast and precise 16 bit ADCconverters and realtime data acquisition and display.

02HIGHLIGHTSCyFlow ML Flow Cytometry Systemultracompact desktop high end instrumentdimensions [cm]: L 56 x H 30 x D 65 (stand-alone)16 optical parameters: FSC1, FSC2, SSC, FL1-FL134 light sources: triple laser configurations includingnew powerful 200mW@488nm blue solid state laser 100 W UV lamp for highest resolution DNA analysisWindows XP FloMax software for realtime dataacquisition, data display and data evaluationparallel 16bit digital pulse processingflexible and modular system configurationssubmicron particle detection ( 0.2µm) for scatterhigh fluorescence sensitivity 100 MEFL

03TECHNOLOGYCyFlow ML Flow Cytometry SystemThe ultracompact desktop instrument CyFlow MLprovides 4 light sources and 16 optical parameters foruse in any application in research and laboratory routine.The optical system of the CyFlow ML features a clear optical16 optical parameters, each with its own photomultiplierpathway, built upon Partec’s legendary optical cubes. Alltube (PMT), including two forward scatter PMTs and sideoptical elements, including the lasers and the UV mercuryscatter PMT, give you the most flexible choice and offer the bestlamp, are mounted onto a single rigid optical bench, whichsensitivity as well as enough space for future applications.guarantees optimum mechanical stability and avoids problems when moving the instrument to another place.Employing 4 light sources including3 solid state lasers and the UV lampFeaturing unique fluorescence sensitivityand scatter resolutionThe light source system of the CyFlow ML in its standard By avoiding fiber coupling, the performance of the CyFlow MLconfiguration comes up with a new powerful 200mW@488 nm is characterized by a superior fluorescence sensitivity ofblue solid state laser, 25 mW @ 635 nm red diode laser, 100 MEFL (see figure below on the left) and by going to100 mW @ 532 nm green solid state laser and the 100 W UV nano-resolution in the scatter (see figure below on the right).lamp, all with direct light coupling for maximum sensitivity. Any application focussing on small particle detection andOther laser light sources are optionally available (violet and analysis and requiring highest fluorescence intensity, fluorescenceUV lasers), therefore offering full upgrade possibilities. Due sensitivity as well as scatter resolution benefits from the unito the built-in colour CCD camera, the stability of the sample que performance charateristics of the CyFlow ML.flow can be visually controlled by a video flow monitor.

LAMP BEAM (UV)FORWARD SCATTERHIGH NA OBJECTIVESRED DIODE LASERGREEN SOLID STATE LASERBLUE SOLID STATE LASERSHEATH FLUID (HYDRODYNAMIC FOCUSING)SAMPLEThe unique Partec quartz flow cuvette is the heart of theCyFlow ML, ensuring that particles and cells cross the UVand visible excitation light with best possible precision.The design of the flow cuvette incorporates more than 35precision DNA measurements for tumor or chromosomeyears of experience in handling fluids with sub-micrometeranalysis. The sample is transported with help of a computerand nanoliter precision. Thanks to the optical and mechani-controlled digital syringe pump, part of a virtually cross-con-cal precision of the flow cuvette, superior results are guar-tamination-free fluid system.anteed for all parameters, e.g. coefficients of variation (CVs)of better than 1% with DAPI staining - a prerequisite for high

Laboratories today demand highest flexibility for upcomingapplications. The CyFlow ML‘s open system architecture isdesigned for the future.Taking advantage of most recent computerand electronic technologiesAll-in-one design and modular extensionfor future cytometric analysisEach optical channel is equipped with an independent processorThe CyFlow ML in its ultracompact design incorporates allcontrolled digital pulse analyser for parallel pulse processing inmodules and components within smallest dimensions oforder to minimize signal losses due to coincidence and dead-L 56 x H 30 x D 65 [cm]. In contrast to other available FCMtime effects. 16 bit (65536 channels) analogue-to-digital conver-systems, the CyFlow line of instruments does not requireters for each parameter are the fundament for software-basedany external equipment. Power supply, pressure regulatorscolour crosstalk compensation algorithms and other numericaland the fluidic system are completely built-in. Additionaloperations, e.g. fluorescence ratio measurements, without arti-space beside or under your laboratory desk is not any longerfacts. The computing power of the CyFlow ML system allowsrequired. In order to be prepared for future applications, thesignal analysis, processing and display of each event generatedCyFlow ML offers flexible and modular configurations in aby a particle in realtime, which is a prerequisite for precise highmost compact system architecture.speed analysis and accurate absolute counting.

Computer Controlled MotorDigital Velocity MeterStartSyringe PumpFlow CuvetteStopSample Tubec NVStartCountStopThe True Volumetric Absolute Counting (TVAC) is aunique feature of all Partec Flow Cytometers, offeringhighest absolute counting precision and accuracy.The CyFlow ML analyses concentrations of any particle ordiameter. Alternatively, a well defined volume of free choicecell subpopulations of interest using True Volumetric Absoluteinvolving the digital sample speed control can be used.Counting. This unique method is solely based on the funda-Benefits of True Volumetric Absolute Counting:mental scientific definition of absolute counting resp. theparticle concentration c, namely the counted number N ofparticles (events) in a given volume V, c N / V. In theCyFlow ML, the volume is measured directly by mechanicalmeans, rather than by calibration with expensive beads witha - sometimes doubtful - „given“ nominal concentration. Thus,the precision of volume measurement is defined by a fixedmechanical design, eliminating any errors related to varyingbead concentrations. The CyFlow ML allows analysis of adigital volumetric precision by mechanicaldesign: CV 2%no errors related to calibrationno additional time and preparation stepsfor reference beads or haematologyreference countno expenses for calibration beadsno separate haematology counter requiredfixed volume as defined by the distance between two platinum electrodes reaching into the sample tube with a givenSelection of Fluorochromes for the CyFlow ML Light Source SystemType of laserFluorescence ChannelFluorochromesGREEN0RANGEORANGE REDREDINFRA REDFITCGFPPEeYFPPE-Texas Red PE-Cy5PE-Cy5.5PE-Cy7635 nmREDINFRA REDAPCAPC-Cy7green solid state laser 532 nm0RANGEREDINFRA lue solid state laser 488 nm200 mWred diode laserAPC-Cy5.5Alexa Fluor 488SYTO 9PIPerCPECDSYTO 59-61PerCP-Cy5.5Alexa Fluor 633Alexa Fluor 64725 mW100 mWUV lamp366 nm100 W(200-600 nm)other lasers optionalAlexa Fluor 532PE-Cy5.57-AADHoechstSYTO 80-83PIAlexa Fluor 350CY 3AMCAEB

04ANALYZECyFlow ML Flow Cytometry SystemThe Windows XP FloMax software integrates instrumentcontrol including acquisition, on- and offline data analysis, onand offline compensation into a complete software package.Ready prepared and freely adaptable instrument settingsAbsolute Counting feature of the CyFlow ML, displayingand panels facilitate switching between different applica-particle concentrations for any subsets of cells, even if defi- tions. FloMax is optimized for immunophenotyping, micro-ned by a gate at a later time after the acquisition.biology analysis, cell cycle, DNA ploidy, and scientific flowcytometric analysis. Data is stored in FCS flow cytometrystandard file format for easy exchange with other analysissoftware. One of the unique features is the digital on- andoffline colour crosstalk compensation of the spectral overlapof fluorescence from simultaneously analysed dyes. The Ncolour compensation algorithm allows a correction of thecrosstalk between any parameters without the need to reruna sample. FloMax optimally supports the True VolumetricFull flexibility and automation by the multi-tube FloMax panel systemand the FloMax Report system within the FloMax software package.The flexible FloMax panel system allows automated analy-easy-to-adapt automated single or multi-tube panel results.sis of repeating sample series employing different dyes orReports are based on Microsoft Word or Excel written in instrument settings. The FloMax Report system generatesVisual Basic.