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Standard analysiscolumnsColumn Selection for Size ExclusionChromatography (SEC)ApplicationSolventAnalysis of biological macromolecules(proteins, peptides, nucleic acids, etc.)buffer etc.buffer etc.KW400 seriesAnalysis of biological macromolecules(high MW range)buffer etc.SB-800 HQ seriesbuffer etc.SB400 seriesWater-soluble polymers(polyacrylamide, polyethylenimine, etc.)H2O, bufferaqueoussolution etc.SB-800 HQ seriesH2O,aqueoussolutionKS-800 series32GS-HQ series48THFKF-800 seriesAqueous SEC(GFC)Oligosaccharide, polysaccharides42ColumnKW-800 seriesSB400 seriesColumn Selection for Size Exclusion Chromatography (SEC)Organic SEC(GPC)Analysis at high temperature(polyethylene, polypropylene etc.)Engineering resin analysis at roomtemperature[polyamide (nylon), polyethyleneterephthalate (PET) etc.]4446High performance(solvent-saving)4646High performance(solvent-saving)4652LF series58KF-600 seriesRapid analysis(solvent-saving)56THFKF-400HQ seriesHigh performance(solvent-saving)56chloroformK-800 series52DMFKD-800 series54DMFSB-800 HQ series46DMFSB400 series46ODCB etc.HT-800 series58ODCB etc.UT-800 series58ODCB etc.AT-806MS58HFIPHFIP-800 seriesTHFPolar polymers(polyimides, polyvinylpyrrolidones etc.)44High performance(solvent-saving)High linearity ofcalibration curveTHFAnalysis of general polymersPageHFIPHFIPAqueous/OrganicSEC60HFIP-600 seriesRapid analysis(solvent-saving)60LF-404High performance(solvent-saving)5850GF-HQ seriesGuideline of AvailabilityPolarity of oroformKW-800 series, KW400 seriesSB-800 HQ series, SB400 seriesGS-HQ seriesGF-HQ seriesKD seriesKF, K, LF series* See page 62 for the solvent replaceability of organic solvent SEC (GPC) packed columns.Toluene
InformationPrecautions for polar polymer analysisSize exclusion chromatography analysis of polar-polymer can be influenced by unexpected interactions in the column.These interactions will result in changing elution patterns and may cause the invalid molecular weight calculations.It is important to reduce them in order to obtain the accurate molecular weight distribution.Interfering interactions likely to be observedInteractions between the analyte and the packing materialsElution will beretarded.log MWExclusion limitMWElution will eInteraction within and between the analyteIonic repulsion effects observed within the multivalentmacromolecules causes structure expansionThis accelerates the analyte elution, and thus results inoverestimation of its molecular weight (Figure A).Association between the moleculesAssociated molecule detected as a larger molecule(Figure A).(D)(C)Interactions between the analyte and the solventThe multivalent ion of the solvent works as a bridge tobind ionic molecules (analyte).Methods to reduce interactionsOrganic solvent SEC (GPC) columnsIonic InteractionAdd salt(Example) Add LiBr to DMFAdd CF3COONa to HFIPHydrophobic interactionLower the polarity of the eluent(Example) Change the eluent from DMF to THFHydrophillic interactionIncrease the polarity of the eluent(Example) Change the eluent from THF to DMFAqueous SEC (GFC)Ionic InteractionAdd saltHydrophobic interactionIncrease dissociation of the analyteCationic polymerLower the pHAnionic polymerHigher the pHLower the eluent polarity(Example) Add acetonitrile or methanol43Precautions for polar polymer analysisHydrophobic interactionThe analyte is adsorbed into the packing material.This delays the analyte elution, and thus results inunderestimation of its molecular weight (Figure B, D).Ionic interaction(1) Ion ExclusionThe analyte is repelled from the packing material.This accelerates the analyte elution, and thus resultsin overestimation of its molecular weight (Figure A, C)(2) Ion ExchangeThe analyte is adsorbed onto the packing material.This delays the analyte elution, and thus results inunderestimation of its molecular weight (Figure B, D).
Standard analysiscolumnsAqueous SEC (GFC) columns : Silica-basedFeaturesKW-800Silica-based packed columns for aqueous SEC (GFC) analysisNotebookSuitable for the analysis of proteins and nsp.85Reducing particle size of the packing material enhances column performanceThree- or four-fold higher sensitivity than KW-800 seriesKW405-4F is applicable to samples with a molecular weight above 1 millionNotebookNo.5, 7Semi-microMicro Columnsp.77Standard columns44Aqueous SEC (GFC) columns : Silica-basedParticleSize(µm)Exclusion LimitShippingSolvent4008.0 300H2O51,0008.0 300H 2O71,5008.0 300H 2O7 6.0 50H 2OProduct NamePlate Number(TP/column)F6989000PROTEIN KW-802.5 21,00060,000150,0005F6989103PROTEIN KW-803 21,000170,000700,000F6989104PROTEIN KW-804 16,000500,000(1,000,000)*F6700131PROTEIN KW-G(Pullulan)(Protein) (guard column)MaximumPore Size(Å)Column Size(mm)I.D. x LProduct CodeBase Material: SilicaUsable pH range : pH3.0-7.5The maximum usable concentration is100% for methanol and acetonitrile.* Estimated value within parenthesisHigh performance semi-micro columnsProduct CodeProduct NamePlate Number(TP/column)ParticleSize(µm)Exclusion Limit(Pullulan)(Protein)MaximumPore Size(Å)Column Size(mm)I.D. x LShippingSolventF6989201KW402.5-4F 35,00060,000150,00034004.6 300H 2OF6989202KW403- 4F 35,000150,000600,00038004.6 300H 2OF6989203KW404- 4F 25,000500,000(1,000,000)*51,5004.6 300H 2OF6989204KW405- 4F 25,0001,300,000(20,000,000)*52,0004.6 300H 2OF6700132KW400G -4A(guard column) 5 4.6 10H 2OBase Material: SilicaUsable pH range : pH3.0-7.5The maximum usable concentration is100% for methanol and acetonitrile.* Estimated value within parenthesisMolecular weight range with protein (eluent : phosphate buffer)Molecular weight (Protein)210103410510106107KW-802.5, 402.5KW-803, 403KW-804, 404KW405CalibrationStandardsSee page 63 forCalibration Standards*Contact Shodex or our distributors near you for customized columns.108
Recovery rate of proteinsRecovery (%)Protein107107KW402.5-4F lecular weight (Protein)Molecular weight ochrome c9292Lysozyme8798α-Chymotrypsinogen A9594ColumnEluent: Shodex KW402.5-4F, KW403-4F: 50mM Sodium phosphate buffer 0.3M NaCl(pH7.0)Flow rate: 0.33mL/minDetector: UV(280nm) (small cell volume)Column temp. : 25 CComparison of KW402.5-4F and KW-802.5Milk whey in yogurtKW400 series are high-performance semi-micro columnswith theoretical plate number which is approximately 1.5times larger and a detection sensitivity (peak height) thatis 3 to 4 times higher than what KW-800 columns have.LectinsSample : 5µL1. Lectin from Soybean2. Lectin from Arachis hypogaea3. Lectin from Canavalia ensiformis (Con A)4. Lectin from Lens culinaris (LCA)5. Lectin from Triticum vulgaris (WGA)Sample : Whey, 5µL553N /mL5(B) KW-802.51 23N 8,2004501015 min20: (A) Shodex KW402.5-4F(B) Shodex PROTEIN KW-802.5Eluent: 50mM Sodium phosphate buffer 0.3M NaCl(pH7.0)Flow rate: (A) 0.33mL/min, (B) 1.0mL/minDetector: UV(280nm) (small cell volume)Column temp. : 25 C30405050 min10 minColumnColumnEluent: Shodex KW402.5-4F KW403-4F: 50mM Sodium phosphate buffer 0.3M NaCl(pH7.0)Flow rate: 0.20mL/minDetector: UV(280nm) (small cell volume)Column temp. : 30 CComparison of standard protein separation usingvarious GFC columns: Shodex KW402.5-4F: 50mM Sodium phosphate buffer 0.3M NaCl(pH7.0)Flow rate: 0.33mL/minDetector: UV(220nm) (small cell volume)Column temp. : 30 CProteins in human blood serumSEC columns for an aqueous system, i.e., SB-803 HQ,GF-510 HQ and KW-803, were compared with eachother in terms of the separation of standard proteins.The result demonstrates that the KW-803, a silica-basedcolumn, is the most suitable for such an application.Sample :A : Thyroglobulin (bovine)B : γ-Globulin (bovine)C : Ovalbumin (chicken)D : Nyoglobin (horse)E : CyanocobalaminColumnEluentSample : Human serum 0.1% each1. Fibrinogen50µL2. α2 -Macroglobulin 50µL3. IgG50µL4. Transferrin50µL5. Plasminogen50µL6. Albumin100µL7. Antitrypsin100µL8. Hemoglobin100µL6D106CBDSB-803 HQMolecular weight (Protein)AECBEDAGF-510 HQBCA1KW-803GF-510 HQSB-803 1 min810: Shodex OHpak SB-803 HQ, Asahipak GF-510 HQ,PROTEIN KW-803Eluent: 0.2M Phosphate buffer(pH6.9)Flow rate: 0.5mL/minDetector: UV(280nm)Column temp. : 30 C1520 min10152030 minColumnColumn:Eluent:Flow rate:Detector:Column temp. :Shodex PROTEIN KW-803 x 250mM Phosphate buffer 0.3M NaCl(pH7.0)1.0mL/minUV(280nm)Room temp.45Aqueous SEC (GFC) columns : Silica-based: Shodex KW400-4F series, Shodex PROTEIN KW-800 series: 50mM Sodium phosphate buffer 0.3M NaCl(pH7.0): (KW400) 0.33mL/min(KW-800) 1.0mL/minDetector: (KW400) UV(280nm) (small cell volume)(KW-800) UV(280nm) (conventional type)Column temp. : 25 C(A) KW402.5-4FBovine serum albumin13ColumnEluentFlow ution volume (mL)Elution volume (mL)Sample : 10µL1. Blue dextran 20002. γ-Globulin3. Ovalbumin4. Myogrobin5. Uridine98KW-8031031021021.5γ- GlobulinStandard analysiscolumnsCalibration curves for KW400 series and KW-800 series
Standard analysiscolumnsAqueous SEC (GFC) columns : Polymer-basedFeaturesSB-800 HQPolymer-based packed columns for aqueous SEC (GFC) analysisSupports a wide range of molecular weightsThe eluent can be substituted with DMF (in columns other than SB-802 HQ andSB-807 HQ), enabling the analysis of polar polymersSB-804HQ and SB-805HQ are capable of determining the mean molecular weightof the gelatin compliant with PAGI method (ver. 10, Japan)NewsNo.29, 39Semi-microMicro Columnsp.78, 79p.85PreparativeColumnsSB-807 HQColumns for the analysis of water-soluble ultra-high molecular polymerPacking materials with a large particle size are packed in order to prevent the breakage of molecularchainsSB400Three- or four-fold higher sensitivity than the SB-800 HQ seriesSB401-4E can analyze small molecules with MW below 1,000NewsNo.38, 39Standard columns46Aqueous SEC (GFC) columns : Polymer-basedProduct CodePlate Number(TP/column)Product NameExclusion Limit(Pullulan)Particle Size(µm)MaximumPore Size(Å)Column Size(mm)I.D. x LShipping SolventF6429100OHpak SB-802 HQ 12,0004,00081008.0 3000.02% NaN3 aq.F6429101OHpak SB-802.5 HQ 16,00010,00062008.0 3000.02% NaN3 aq.F6429102OHpak SB-803 HQ 16,000100,00068008.0 3000.02% NaN3 aq.F6429103OHpak SB-804 HQ 16,0001,000,000102,0008.0 3000.02% NaN3 aq.F6429104OHpak SB-805 HQ 12,000(4,000,000)*137,0008.0 3000.02% NaN3 aq.F6429105OHpak SB-806 HQ 12,000(20,000,000)*1315,0008.0 3000.02% NaN3 aq.F6429106OHpak SB-806M HQ 12,000(20,000,000)*1315,0008.0 3000.02% NaN3 aq.F6709430OHpak SB-G(guard column) 10 6.0 500.02% NaN3 aq.SB-806M HQ is a mixed-gel column capable of analysis ofsamples over a wide range of molecular weight distribution.Base Material : Polyhydroxymethacrylate*( )Estimated valueUsable pH range : pH3 10For water-soluble ultra-high molecular polymerF6429108OHpak SB-807 HQF6709431OHpak SB-807G 1,500(500,000,000)*3530,0008.0 300H2O(guard column) 35 8.0 50H 2OBase Material : Polyhydroxymethacrylate*( )Estimated valueHigh performance semi-micro columnsProduct CodeExclusion Limit(Pullulan)Plate Number(TP/column)Product NameParticle Size(µm)MaximumPore Size(Å)Column Size(mm)I.D. x LShipping Solvent1,00010404.6 250H 2O 20,00010,00062004.6 250H 2O 20,000100,00068004.6 250H 2O 20,0001,000,00072,0004.6 250H 2O7 4.6 10H 2OF6429111SB401-4E 04-4EF6709432SB400G-4A(guard column) Base Material : PolyhydroxymethacrylateUsable concentration of organic solventsProduct NameUsable pH range : pH3 10Molecular weight range with pullulan (eluent : ultrapure water)The maximum usable concentration (%)Methanol AcetonitrileDMFSB401-4E0100SB-802 HQ000SB-802.5 HQ, SB-803 HQSB402.5-4E, SB403-4E10075100SB-804 HQ SB-806M HQ,SB404-4E7575100SB-G, SB400G-4A7575100SB-807 HQ, SB-807G30300Note :The maximum solvent tolerance to the SB-800 HQ series preparativecolumns(SB-2000 series) to methanol, acetonitrile, and DMF is 50%.(SB-2002 is not tolerant to solvents, similar to SB-802 HQ.)CalibrationStandardsUsable pH range : pH3 10See page 63 forCalibration Standards*Contact Shodex or our distributors near you for customized columns.Molecular weight (Pulullan)10102103104105106107108109SB401SB-802 HQSB-802.5 HQ, 402.5SB-803 HQ, 403SB-804 HQ, 404SB-805 HQSB-806 HQSB-807 HQSB-806M HQMolecular weight range with PEG/PEO (eluent : DMF)Molecular weight (PEG/PEO)10102103104105106SB-802.5 HQ, 402.5SB-803 HQ, 403SB-804 HQ, 404SB-805 HQSB-806 HQSB-806M HQ107108109
Standard analysiscolumnsCalibration curves for SB-800 HQ series usingPEG and PEO (DMF)Calibration curves for SB400 series and SB-800 HQ series using pullulan (H2O)1072.02.5Molecular weight (PEG/PEO)Molecular weight (Pullulan)Molecular weight (Pullulan)3.043.556789101141256Elution volume (mL)Elution volume (mL)ColumnEluentFlow - 105107SB107106789101112Elution volume (mL)::::Column:Eluent:Flow rate:Detector:Column temp. :Shodex OHpak SB-800 HQ series20mM LiBr in DMF0.8mL/minRI40 C47CarboxymethylcelluloseSEC analysis of humic substanceSample : Carboxymethylcellulose(Medium viscosity) 0.1%, 200µLThe left graph shows the results of SECanalysis of reference materials (from theJapanese Humic Substances Society) ofhumic acid and fulvic acid prepared frombrown forest soil (Dando) and kurobokusoil (Inogashira).Calibration curve of Sodium polystyrene sulfonateMw 101 x 104M n 10.5 x 104Mw/Mn 9.6Sample : Humic substances, 30μLHumic acid 0.02mg/mLFulvic acid 0.02mg/mLHUMIC ACID(Dando soil)HUMIC ACID(Inogashira soil)FULVIC ACID(Dando soil)FULVIC ACID(Inogashira soil)709111315Vo2468101214 16 18min20222426283017 minVtColumn:Eluent:Flow rate:Detector:Column temp. :Column:Eluent:Flow rate:Detector:Column temp. :Shodex OHpak SB-G SB-805 HQ10mM NaH2PO4 10mM Na2HPO4(pH7.0) 25% CH3CN0.8mL/minUV(260nm)Data provided by Nobuhide Fujitake,40 CProfessor of the Graduate School of Agricultural Science, Kobe UniversityEffect of sodium nitrate concentration ineluent on the analysis of polyallylaminePolycarbonateIn the analysis of cationic polymers such as polyallylamine,the concentration of sodium nitrate in the eluent being0.02M would allow the column to adsorb the mainchain of polyallylamine, resulting in the completeprevention of the elution of polyallylamine. However, ifthe concentration is 0.1M or higher, adsorption of thesample is suppressed and a favorable chromatogramcan be obtained.PolyacrylamideSample : Polyacrylamide, 100µLSample : PolycarbonateSB-806 HQCH3OCCH3O COn1.0x108Molar Mass(g/mol)SB-807 HQSample : Polyallylamine 0.2%, 100µL0.1 MShodex OHpak SB-806M HQ x 20.1M NaCl aq.1.0mL/minRI40 C0.02 M0.2 M1.0x10761.0x10Mw: 5,500,00051.0x104.00.05 0 minVolume (mL)Column: Shodex OHpak SB-806M HQ x 2Eluent: 0.5M Acetic acid NaNO3 aq.Flow rate: 1.0mL/minDetector: RIColumn temp. : 40 CColumn: Shodex OHpakSB-805 HQ SB-802.5 HQEluent: 5mM LiBr in DMFFlow rate: 1.0mL/minDetector: RIColumn temp. : 40 CColumnEluentFlow rateDetector::::Shodex OHpak SB-807 HQ, SB-806 HQ0.2M NaCl aq.0.5mL/minRIMALS(Multi angle laser light scattering)Column temp. : 30 C14.0Aqueous SEC (GFC) columns : Polymer-basedShodex SB400 series, Shodex OHpak SB-800 HQ seriesH2O0.3mL/min, 1.0mL/min(SB400) RI(small cell volume)(SB-800 HQ) RI(conventional type)Column temp. : Room temp.
Standard analysiscolumnsMultimode columnsFeaturesSEC mode is the main modeMultimode columns for reversed phase, HILIC, and ion exchange modes are availabledepending on selected eluent conditionsSuitable for the separation of peptides or nucleic acids with similar molecular weightsSuitable for desalting samples or substituting buffer in protein analysisAsahipak GS-HQNotebookNo.3No.19, 20NewsSemi-microMicro Columnsp.80PreparativeColumnsp.86Asahipak GS-320 7EFor the analysis of nucleic acids as “Umami” and its metabolite with isocratic elutionAsahipak GS-620 7G-PColumns to determine mean molecular weight distribution of gelatin compliant with PAGImethod (ver. 10, Japan)MSpak GS-320Semi-micro columns for Asahipak GS-320 HQEffective for the analysis of chemical substances in a biological sample by columnswitching intended to remove proteinsNews No.2Applicable to analysis using LC/MS or ICP-MS48Multimode columnsStandard columnsProduct CodeProduct NamePlate Number(TP/column)F7600005Asahipak GS-220 HQ 19,000F7600006Asahipak GS-320 HQF7600007Asahipak GS-520 HQF7600008Asahipak GS-620 HQF6710019Asahipak GS-2G 7BExclusion Limit(Pullulan)ParticleSize(µm)Column Size(mm)I.D. x LShipping Solvent1507.5 300H2O/CH3OH 70/30MaximumPore Size(Å)3,000*6 19,00040,00064007.5 300H2O/CH3OH 70/30 18,000300,00072,0007.5 300H2O/CH3OH 70/30 18,000(2,000,000)**77,0007.5 300H2O/CH3OH 70/30(guard column) 9 7.5 50H2O/CH3OH 70/30Base Material: Polyvinyl alcoholUsable pH range : pH2 12(GS-220 HQ : pH 2 9)Usable concentration of methanol is up to 100%(GS-220 HQ up to 30%)Usable concentration of acetonitrile is up to 50%* PEG equivalent**( )Estimated valueMolecular weight range with Pullulan (eluent : ultrapure water)Molecular weight (Pulullan)10210103104105106107GS-220 HQGS-320 HQGS-520 HQGS-520 HQColumns for the analysis of nucleic acids as “Umami”Product CodeProduct NameScope of applicationColumn Size(mm) I.D. x LShipping SolventF7610005Asahipak GS-320 7E“Umami” (nucleic acids)7.5 250H2O/CH3OH 70/30F6710019Asahipak GS-2G 7B(guard column)7.5 50H2O/CH3OH 70/30Columns to determine the molecular weight distribution of gelatinProduct CodeProduct NameScope of applicationColumn Size(mm) I.D. x LShipping SolventF7600023Asahipak GS-620 7G-PGelatin for photo film7.5 500H2O/CH3OH 70/30F6710019Asahipak GS-2G 7B(guard column)7.5 50H2O/CH3OH 70/30Semi-micro columnsProduct CodeProduct NamePlate Number(TP/column)Particle Size(µm)MaximumPore Size(Å)Column Size(mm)I.D. x LShipping SolventF7600130MSpak GS-320 4B 2,00064004.6 50H2OF7600140MSpak GS-320 4D 7,00064004.6 150H 2OF7600150MSpak GS-320 2D 4,00064002.0 1 50H 2OCalibrationStandardsSee page 63 forCalibration Standards*Contact Shodex or our distributors near you for customized columns.
Calibration curves for GS-HQ series usingpullulan and PEGGS-620HQ105GSGS10-5-320420HQHQSample :No.103GS-220102451036HQ789101112Elution volume 73.2411.441Molecular weightMolecular weight106GFCGS-HQ columns can be used not only for SEC (GFC)in an aqueous system, but also for multimodalanalysis where hydrophobic interaction and ionicinteraction are used together as separation criteria,under certain conditions of the eluent. This resultsin unprecedented separation analysis.GS-HQ columns are excellent in the performancefor separating hydrophilic peptides, in particular,acidic or basic peptides, from each other.PullulanPEG63254102pl56231Σ f : Hydrophobic parameterp l : Isoelectric pointColumn:Eluent:Flow rate:Detector:Column temp. :Low molecular weight, water-soluble dietary fiber4Shodex Asahipak GS-320 HQ50mM Ammonium acetate buffer(pH7.0)0.5mL/minUV(220nm)30 C49151020Multimode columnsColumn: Shodex Asahipak GS-HQ seriesEluent: H2OFlow rate: 0.6mL/minDetector: RIColumn temp. : 30 CStandard analysiscolumnsPeptides25 minAnalysis of the purine base in beersMonosaccharides, disaccharides,and sugar alcohols are eluted withretention times larger than thatindicated by the arrow. Thisfacilitates the separation of thosesubstances from indigestiblefractions, making thedetermination of small,water-soluble dietary fiber moreefficient.Purine bodies in food are analyzed after being neutralized to a purine base by hydrolyzing thehomogenized and freeze-dried food with a 70% perchloric acid. This section presents an example ofanalysis of purine base in ordinary beer and purine base in beer treated with guanase (an enzyme thatdegrades guanine to xanthine). The following data indicates that guanine was decreased and xanthinewas increased by guanase.Normal beerGuanase treated olumn:Eluent:Flow rate:Detector:Column temp. :Shodex Asahipak GS-220 HQ x 2H2O0.5mL/minRI60 C1020Column:Eluent:Flow rate:Detector:Column temp. :“Umami”304050 min0Shodex Asahipak GS-320 HQ150mM Sodium phosphate buffer(pH2.5)0.6mL/minUV(260nm)35 C203040Data provided by Prof. Kiyoko Kaneko,Faculty of Pharmaceutical Sciences,Teikyo UniversitySample : 100nL1. γ-Glutamylmethylselenocysteine2. MethylselenocysteineSample : 100µLGelatin(Koepff 16922), 0.2%COOH2Sample :1. IMP2. GMP3. AMP4. Inosine5. Hypoxanthine6. Guanosine37. Guanine28. Adenosine9. Adenine117489Column:Eluent:Flow rate:Detector:Column temp. ys)10152025303540052030 minShodex Asahipak GS-320 7E10mM Sodium phosphate buffer(pH5.0)1.0mL/minUV(260nm)30 CColumnEluent: Shodex Asahipak GS-620 7G-P x 2: 0.1M KH2PO4 aq./0.1M Na2HPO4 aq. 50/50Flow rate: 1.0mL/minDetector: UV(230nm)Column temp. : 50 C1015202530Retention time (min)min10HNH2NO5050 minHigh sensitivity analysis of metal-bindingamino acidsGelatin analysis with PAGI methodA multimode column GS-320 7E supports an easy andsimultaneous analysis of major nucleic acids that arepresent in taste components, i.e., IMP, GMP, andAMP, and derivatives thereof with isocratic elution.10ColumnEluentFlow rateDetector::::Shodex GS320A-M5D50mM CH3COONH4 buffer(pH6.5)2.0μL/minICP-MS(Se at m/z 82)Data provided by Yasumitsu Ogra,Professor of Showa Pharmaceutical University
Standard analysiscolumnsAqueous/Organic SEC columnsFeaturesPolymer-based SEC column exhibits solvent durabilityAsahipak GF-HQSupports both aqueous and organic solventsNewsNo.2Semi-microMicro Columnsp.81p.86PreparativeColumnsSemi-micro columns for Asahipak GF-310 HQMSpak GF-310Effective for the analysis of chemical substances in biological samples by column switchingintending to remove proteinsUseful for LC/MS analysisSuitable for the analysis of surfactants50NewsNo.2Standard columnsAqueous/Organic SEC columnsColumn Size(mm)I.D. x LShipping Solvent4007.5 300H2O/CH3OH 70/3052,0007.5 300H2O/CH3OH 70/30(10,000,000)*910,0007.5 300H2O/CH3OH 70/30 13,000(10,000,000)*910,0007.5 300H2O/CH3OH 70/30(guard column) 9 7.5 50H2O/CH3OH 70/30Plate Number Exclusion Limit(TP/column)(Pullulan)ParticleSize(µm)Product CodeProduct NameF7600001Asahipak GF-310 HQ 19,00040,0005F7600002Asahipak GF-510 HQ 19,000300,000F7600003Asahipak GF-710 HQ 11,000F7600004Asahipak GF-7M HQF6710018Asahipak GF-1G 7BMaximumPore Size(Å)Base Material: Polyvinyl alcoholUsable pH range : pH2 9*( )Estimated valueGF-7M HQ is a mixed-gel column capable of analysis of samplesover a wide range of molecular weight distribution.Usable solventsWater(sodium concentration)0 0.5MMethanol0 100%Ethanol0 100%Acetonitrile0 100%THF0 100%DMF0 100%Acetone0 100%Chloroform0 100%Ethylacetate0 100%DMSO0 50%Molecular weight range with pullulan (eluent : ultrapure water)Molecular weight (Pulullan)10102103104105106107108106107108GF-310 HQGF-510 HQGF-710 HQGF-7M HQMolecular weight range with PEG/PEO (eluent : DMF)Molecular weight (PEG/PEO)10102103104105GF-310 HQGF-510 HQDMSOGF-710 HQGF-7M HQ51 100%Note : The solvents used for preparative columns GF-710 HQ (GS-710 20F, 20G) are water and methanol.Selection of GSM-700 20F or 20G is recommended when other solvents are used for scale-up testing with GF-710 HQ.Semi-micro columnsProduct NamePlate Number(TP/column)Particle Size(µm)MaximumPore Size(Å)Column Size(mm)I.D. x LShipping SolventF7600100MSpak GF-310 4B 3,00054004.6 50H 2OF7600110MSpak GF-310 4D 10,00054004.6 150H 2OF7600024MSpak GF-310 4E 16,00054004.6 250H 2OF7600120MSpak GF-310 2D 5,50054002.0 150H 2OProduct CodeCalibrationStandardsSee page 63 forCalibration Standards*Contact Shodex or our distributors near you for customized columns.
Calibration curves for GF-HQ series usingpolystyrenePolyacrylonitrilePullulanPEGSample : Polyacrylonitrile 0.1%(w/v), 100µLMolecular weight ar weight106HQ3101024567891041157Column:Eluent:Flow rate:Detector:Column temp. :Shodex Asahipak GF-HQ seriesH2O0.6mL/minRI30 C910110Shodex Asahipak GF-HQ seriesChloroform0.6mL/minUV(254nm)30 CComparison of standard polystyrene separation using various solvent5(B) Chloroform1015Column:Eluent:Flow rate:Detector:Column temp. :2025303540 minShodex Asahipak GF-710 HQ x 220mM LiBr in DMF0.6mL/minRI40 CSodium polystylene sulfonateSample : 1mg/mL each, 50µLPolystyrene1. MW : 1,090,0002. MW : 190,0003. MW :37,9004. MW :9,1005. MW :2,9806. MW :5001(A) THF8(C) DMFPolymers having both hydrophobic andhydrophilic moieties may exhibithydrophobic interactions with packingmaterials. When using such polymers,SEC analysis can be performed byeliminating the hydrophobic interactionthrough the addition of organic solventsto the eluent.MW:104,0001120102030 minSample : 1mg/mL each, 50µLSodium polystylene sulfonate324323456520 min0620 minCH2450CHMW:38,000061020minSO3Nan20 minColumnColumn:Eluent:Flow rate:Detector:Column temp. :Triglycerides: Shodex AsahipakGF-510 HQ: 50mM LiCl aq./CH3CN 60/40Flow rate: 0.6mL/minDetector: UV(254nm)Column temp. : 30 CEluentShodex Asahipak GF-310 HQ GF-510 HQ(A); THF, (B); Chloroform, (C); DMF0.5mL/min(A),(B) UV(254nm), (C) UV(270nm)30 CMW:8,0000102030 minNonylphenol ethoxylateSample : 0.25% each, 20µL1. Tristearin2. Trilaurin3. Tributylin4. Triacetin1GF-310 column is suitable for qualitative analysis andquantitative analysis of surfactants to be performed byadjusting the acetonitrile concentration in the eluent.Sample :100mg/L Nonylphenol ethoxylate, 20μL(A) Eluent : H2O/CH3CN 70/30(B) Eluent : CH3CN23451015 min0Column:Eluent:Flow rate:Detector:Column temp. :Shodex Asahipak GF-310 HQTHF0.6mL/minRI30 C20406080 minColumn: Shodex Asahipak GF-310 HQEluent: (A); H2O/CH3CN 70/30, (B); CH3CNFlow rate: 0.6mL/minDetector: UV(220nm)Column temp. : 40 C5101520 min51Aqueous/Organic SEC columnsColumn:Eluent:Flow rate:Detector:Column temp. :6Elution volume (mL)Elution volume (mL)0Standard analysiscolumnsCalibration curves for GF-HQ series usingpullulan and PEG
Standard analysiscolumnsOrganic SEC (GPC) columns(General Analysis) : THF, chloroformFeaturesStandard organic solvent SEC (GPC) columnSupports a wide range of applications from low to high-molecular-weightcompoundsKF-800, K-800PreparativeColumnsp.86, 87Standard columnsKF-800 series : Shipping solvent tetrahydrofuran(THF)Product Code52Plate Number(TP/column)Product NameExclusion Limit Particle Size(PS)(µm)MaximumPore Size(Å)Column Size(mm) I.D. x LOrganic SEC (GPC) columns (General Analysis) : THF, chloroformF6028010GPC KF-801 18,0001,5006508.0 300F6028020GPC KF-802 18,0005,00061508.0 300F6028025GPC KF-802.5 18,00020,00063008.0 300F6028030GPC KF-803 18,00070,00065008.0 300F6027030GPC KF-803L 18,00070,00065008.0 300F6028040GPC KF-804 18,000400,00071,5008.0 300F6027040GPC KF-804L 18,000400,00071,5008.0 300F6028050GPC KF-805 11,0004,000,000105,0008.0 300F6027050GPC KF-805L 11,0004,000,000105,0008.0 300F6028060GPC KF-806 11,000(20,000,000)*1010,0008.0 300F6028090GPC KF-806M 13,000(20,000,000)*1010,0008.0 300F6027060GPC KF-806L 11,000(20,000,000)*1010,0008.0 300F6028070GPC KF-807 6,000(200,000,000)*1820,0008.0 300F6027070GPC KF-807L 6,000(200,000,000)*1820,0008.0 300F6700300GPC KF-G(guard column) 8 4.6 10F6709350GPC KF-800D(solvent-peak separation column) 10 8.0 100*( )Estimated valueK-800 series : Shipping solvent chloroformProduct CodePlate Number(TP/column)Product NameExclusion Limit Particle Size(PS)(µm)MaximumPore Size(Å)Column Size(mm) I.D. x LF6028110GPC K-801 18,0001,5006508.0 300F6028120GPC K-802 18,0005,00061508.0 300F6028125GPC K-802.5 18,00020,00063008.0 300F6028130GPC K-803 18,00070,00065008.0 300F6028194GPC K-803L 18,00070,00065008.0 300F6028140GPC K-804 18,000400,00071,5008.0 300F6028195GPC K-804L 18,000400,00071,5008.0 300F6028150GPC K-805 11,0004,000,000105,0008.0 300F6028196GPC K-805L 11,0004,000,000105,0008.0 300F6028160GPC K-806 11,000(20,000,000)*1010,0008.0 300F6028190GPC K-806M 13,000(20,000,000)*1010,0008.0 300F6028197GPC K-806L 11,000(20,000,000)*1010,0008.0 300F6028170GPC K-807 6,000(200,000,000)*1820,0008.0 300F6028198GPC K-807L 6,000(200,000,000)*1820,0008.0 300F6700401GPC K-G(guard column) 8 4.6 10F6709450GPC K-800D(solvent-peak separation column) 10 * See page 54 for details of the solvent-peak separation columns. The columns with ‘L’or ‘M’ at the end of the column name aremixed-gel columns capable of a wide molecular weight distribution range of samples.8.0 100*( )Estimated valueMolecular weight range with polystyrene (eluent KF-800 series : THF, K-800 series : chloroform)Molecular weight (Polystyrene)10102103104105106Molecular weight (Polystyrene)107108109KF-, K-80110102103104105106KF-, K-803LKF-, K-802KF-, K-804LKF-, K-802.5KF-, K-805LKF-, K-803KF-, K-806MKF-, K-804KF-, K-806LKF-, K-805KF-, K-807LKF-, K-806KF-, K-807*Contact Shodex or our distributors near you for customized columns.107108109
Calibration curves for KF-800L (linear type)series using PS standardPolycarbonate resinSample : Polycarbonate resin 0.1%, 100µL108108107Molecular weight lecular weight (Polystyrene)0780KF3-802KF.580KF2-801103O CO nL07-8LKF 806LKF 805KFKFCH3CCH3O107KF-8106105Standard an
Column Selection for Size Exclusion Chromatography (SEC) * See page 62 for the solvent replaceability of organic solvent SEC (GPC) packed columns. High performance . column, is the most suitable for such an application. Comparison of standard protein separation using various GFC columns. 46 10 10 2 10 3 10 4 10 5 10 6 10 7 10 8 10 9 SB401
