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USER GUIDEMini Blot ModuleOnline SpecialsFor transfer of proteins using the Mini Gel TankCatalog Number B1000Publication Number MAN0007710Revision A.0For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.Information in this document is subject to change without notice.DISCLAIMERLIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH RESPECT TOTHIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY,FITNESS FOR A PARTICULAR PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT ALLOWED BY LAW, IN NOEVENT SHALL LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) BE LIABLE, WHETHER IN CONTRACT, TORT,WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE,MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDINGBUT NOT LIMITED TO THE USE THEREOF.IMPORTANT LICENSING INFORMATIONThis product may be covered by one or more Limited Use Label Licenses. By use of this product, you accept theterms and conditions of all applicable Limited Use Label Licenses.TRADEMARKS 2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientificand its subsidiaries unless otherwise specified.2Mini Blot Module User Guide
ContentsProduct Contents .4Description of Parts .5Before Starting .7Protein Transfer Protocol . 13Maintainance . 21Troubleshooting . 22Appendix A . 24Technical Support . 24Related Products . 25Appendix B . 26Buffer Recipes . 26Appendix C: Safety . 27Safety Information . 27Mini Blot Module User Guide3
Product ContentsMini Blot ModuleThe Mini Blot Module is a simple apparatus designed for blotting of mini gels andis easily inserted into the Mini Gel Tank in place of a gel cassette assembly. TheMini Blot Module is a semi-wet transfer unit. Each module can be used to performprotein transfer from one mini gel using only 250 mL of 1X transfer buffer.ProductcomponentsThe components included with the Mini Blot Module are listed below.ComponentsQuantityMini Blot Module (consisting of anode core and cathode core)B
for NuPAGE Bis-Tris or Tris- . Non-Reduced: NuPAGE Transfer Buffer (20X) 50 mL : 50 mL . NuPAGE Antioxidant . 1 mL — Methanol* 100 mL : 100 mL . Deionized Water : 849 mL . 850 mL : Total Volume . 1000 mL : 1000 mL * 1X Transfer Buffer with 10% methanol provides optimal transfer of a single gel in the blot module.
