WIXLIB

All you need for PCR A guide to instruments, reagents and consumablesIssue 1 - June 2006vwr.comThermal cyclersincluding real timeinstruments plus allassociated reagentsOligonucleotidesand downstreamprocessing of PCRproductsConsumablecompatibilitydata withcommon usemachinesAdhesive and heatplate seals andaccessory equipmentFor PCR , RT-PCR , qPCR and qRT-PCR

Polymerase ChainReaction (PCR )IntroductionThe intention of this guide is to provide a single document for all products needed to successfullyperform PCR -based applications. For this purpose we have collected together appropriateproducts from our extensive range and put them into the guide. All suppliers presented in thiscatalogue hold the PCR license owned by Hoffman-La Roche Inc. Please note that the productselection may be subject to change.We have divided the guide into areas of different PCR -based applications, to make it easy to findwhat you need. In addition, there are sections on oligonucleotides, downstream processing ofPCR products and PCR consumables, including an instrument/plate compatibility chart. Eachsection begins with a short introduction to the application, and is divided into subsections where theproducts are listed. The manufacturers are presented in alphabetical order.A small number of products have restricted availability in Europe. If there are any queries pleasecontact your local VWR sales team.tab l e o f c o n t e n t sPCR is a patented process of Hoffman-LaRoche and requires a license.EditorVWR International LtdCustomer Service CentreHunter BoulevardMagna ParkLutterworthLeicestershireUNITED KINGDOMLE17 4XNInstrumentsThermal Cyclers. 3Real-Time PCR Cyclers. 6PCR Conventional Thermostable Polymerases. 7Hot-Start Polymerases. 8Hi-Fi Polymerases. 10Buffers & dNTPs. 13Master Mixes. 14Pre-aliquoted Master Mixes. 15RT-PCR Reverse Transcriptases. 16First-strand Synthesis Systems. 17One-step RT-PCR Systems. 18RT-Primers. 20RT-Accessories. 20q- and qRT-PCR q and qRT-PCR reaction mixtures presentedaccording to compatibility with realtime instruments. 21OLIGOSInvitrogen . . 31Operon. 31Thermo Electron. 31PCR ConsumablesCompatibility Chart: Thermal Cyclers . . 32/33& Real-Time PCR Instruments / PlatesTubes & Strips. 34Plates. 36Plate Seals. 37Downstream ProcessingPCR Clean-Up Systems. 38Molecular Weight Markers. 40PCR Cloning Vectors. 42Accessory EquipmentSealers. 43CopywritingVWR International Europe bvbaLayout and typesettingThe Graphic Design House, Portsmouth, UKPrintingBishops Printers, Portsmouth, UKNo part of this publication may be reproduced or copiedwithout prior permission by writing of VWR International Europe.Run75.500 copiesPublication date: June 2006 VWR A l l y o u n e e d f o r P C R

PCR instrumentsThermal cyclersThermal cyclers have become part of the basic arsenal of equipmentfor most life science laboratories. Because of their widespread use,the requirements regarding versatility, convenience, speed and level ofautomation differ dramatically.To be able to supply laboratorieswith suitable equipment, we offer awide range of PCR instruments fromtwo world renowned manufacturers:Eppendorf and Thermo ElectronCorporation. This span in productssatisfy the demands for mostapplications from small scale manualPCR to high throughput and highlyautomated amplification of DNA.The table below is an overview of our instruments. In the following sections we highlight twoinstrument systems and present them in detail. If you are interested in detailed informationregarding other PCR machines from our suppliers, we will be happy to provide it. Please send arequest to your local sales office.Thermal CyclersCat. No.ManufacturerDescriptionEU ermo ElectronThermo ElectronThermo ElectronThermo ElectronThermo ElectronThermo ElectronThermo ElectronThermo ElectronMastercycler ep gradient and control panel with manual lidMastercycler ep gradient and control panel with motorised lidMastercycler ep 384 and control panel with manual lidMastercycler ep 384 and control panel with motorised lidMastercycler ep gradient S and control panel with manual lidMastercycler ep gradient S and control panel with motorised lidMastercycler ep gradient with manual lidMastercycler ep gradient S with manual lidMastercycler ep gradient with motorised lidMastercycler ep gradient S with motorised lidMastercycler ep gradient 384 control panelMastercycler ep gradient 384 with manual lidMastercycler ep gradient 384 with motorised lidMastercycler Mastercycler gradientMastercycler personalPx2 Thermal Cycler chassis, supplied without blockGradient Block 0,2 ml interchangeable block modulefor PX2 Thermal Cycler0,2 ml interchangeable block module for PX2 Thermal CyclerPxE Thermal Cycler with 0,2 ml blockPxE Thermal Cycler with 0,5 ml blockPCR Sprint Thermal Cycler with 0,2 ml blockPCR Sprint Thermal Cycler with 0,5 ml blockPCR software and satellite unitCat. No.UK Plug732-0117 732-0411732-0119 732-0412732-0478 732-0413732-0121 732-0414732-0120 732-0415732-0127 732-0416732-0122 732-0117732-0125 732-0415732-0119 732-0412732-0121 732-0414732-0478 732-0413732-0120 732-0915732-0122 ercycler ep family from EppendorfThe Mastercycler ep family from Eppendorfcontains innovative state of the art applianceswith extremely fast and accurate heatingand cooling. These exceptionally fast andreliable modules can be used as stand alonedevices or controlled from either a separatecontrol panel or a PC. Up to 5 cyclers, runningindividual programs, may be connectedto each control panel. The machines areequipped with a new technology for lidheating that avoids the preheating of samplesand thus minimises unspecific annealing. AllMastercycler ep modules are available with anoptional motorised lid for integration into fullyautomated environments. An extremely fast,accurate and user friendly system for virtuallyany PCR application.VWR A l l y o u n e e d f o r P C R

Mastercycler ep familyfrom EppendorfMastercycler ep familyMastercycler ep gradientMastercycler ep gradient SMastercycler ep 384Sample capacity96 x 0,2 ml PCR tubes or 1 PCR plate 8 x 121 PCR plate 384(unskirted, semi-skirted, skirted - according to SBS standard)Temperature control range of the block4 C to 99 CTemperature control modeBlock control, (simulated) tube control; both also available in gradient operation modeHeating technology of the block Peltier elements, Triple Circuit TechnologyGradient blockOver 12 rowsOver 24 rowsGradient range1 C to 20 C1 C to 24 C1 C to 20 CGradient temperature range30 C to 99 CLid temperature range37 C to 110 CLid descent and closing pressure ESP technology, max. of 20 kg / PCR plateBlock homogeneity 20 C to 72 C 0,3 CBlock homogeneity 95 C 0,4 CControl accuracy 0,2 CHeating rate*approx. 4 C/sapprox. 6 C/sapprox. 4 C/sCooling rate*approx. 3 C/sapprox. 4,5 C/sapprox. 3 C/sInterfaces1 x Centronics, 1 x RS 232, Control panel, one each of CAN in/ CAN outDimensions (W x D x H)26 cm x 41 cm x 30,5 cmWeight17 kgPower supply, consumption230 V, 50-60 Hz; 800 W* Measurements on the block.Complete information on Eppendorf cyclers is available in our catalogues or on the VWR websiteMBS Satellite Thermal Cycler from Thermo Electron CorporationHighly flexible, competent and easyto use system for performing PCR on any scale.Thermo Electron corporation offers the MBS Satellite Thermal Cycler. This is a very flexible systemdesigned to satisfy the demands of any lab, from small scale basic PCR to high throughput PCR with GLP standards and/or automation. Furthermore the system offers the latest in satellite blocktechnology, user friendly software and PC control.MBS Satellite Thermal Cycler (MBS)Temperature Control AvailableBlock Temperature RangeBlock Heating RateBlock Cooling RateBlock UniformityHeated Lid Temperature RangePrecision ControlStandard AccessoriesAvailable Satellite Block ModulesDimension of ModulesPowerWeight VWR A l l y o u n e e d f o r P C R Active Tube ControlSimulated plate controlSimulated tube controlBlock control4 C - 99 CUp to 3 C/secUp to 2 C/sec 0,4 C within 15 secs95 C - 120 C0,1 CTube thermistor for 0,2 ml or 0,5 ml blocks0,2 ml (standard and gradient)0,5 ml (standard and gradient)384 well block200 H x 300 W x 290 D (mm)550 W8,4 kg

For more information on these productsvisit our website www.vwr.comPx2 Thermal Cycler from Thermo Electron CorporationThe Px2 Thermal Cycler isthe most advanced personalthermal cycler developed byThermo to date. It offershighly accurate temperaturecontrol and user friendlysoftware.Features include: maximum versatility by offeringa choice of six interchangeableblocks, including:- the latest gradient cycling format- adjustable heated lid for flexiblechoice of consumable type.The Px2 is controlled via a user friendly interface consisting of a large high definition display, anew keypad design with soft keys and intuitive software. Concerning performance, the Px2 deliveroutstanding well to well uniformity for reproducible PCR performance and gradient capabilityfor rapid protocol optimisation. Taken together, this ensures the Px2 to be an excellent choice forscientists using PCR as an integral technique in their laboratories.Px2 Thermal CyclerTemperature control availableBlock uniformityRamping speedPrecise controlBlock temperature rangePortsDisplayProgramsInterchangeable block optionsDimensionsPowerActive tube controlSimulated plate controlSimulated tube controlSimulated slide controlBlock control 0,4 C within 15 secondsUp to 3 C / second0,1 C4 - 99 CRS232¼ VGA (320 x 240)9996 well 0,2 ml (standard and gradient),96 well 0,5 ml (standard and gradient),384 well,In situ flat block240 W x 390 D x 280 H (mm)550 WVWR A l l y o u n e e d f o r P C R

Real Time PCR InstrumentsMastercycler ep realplex from EppendorfLATEST!To meet the demands of the ever growingnumber of quantitative PCR applications,Eppendorf has developed Mastercycler ep realplex. The system features the latesttechnology with an optical detection modulethat promotes consistently high sensitivity inreal-time PCR experiments. This is achievedpartly by using an innovative system with aminimal number of moving parts in the opticaldetection module. Two versions of thermoblocks are available, a “standard” and a“high speed” alternative. Both blocks can becombined with two different optical modules,realplex2 and realplex4, which permit the useof nearly all fluorescent dyes used in real-timePCR . Pre-established assays optimised for realtime PCR reagents and your preferred tubes orplates can easily be transferred to the system. Inconclusion, Mastercycler ep realplex is amulti-competent device for performing real-timePCR with virtually any detection chemistry,including multiplex applications.Mastercycler ep realplexOptical moduleExcitation sourceEmission filtersDetectorDynamicSensitivityThermo moduleSample capacityTemperature control range of blockDegree range of gradient, max.Temperature control range of gradientTemperature of lidBlock homogeneityControl accuracyHeating speed *Cooling speed *Complete systemDimensions /(W x D x H)Total weightWeight of thermomoduleWeight of detection moduleVoltage requirementsPower consumption* Measured at block VWR A l l y o u n e e d f o r P C R 96 LEDs (470 nm)520 / 550 nm (realplex2)520 / 550 / 580 / 605 nm (realplex4)1-channel photomultiplier tube (realplex2)2-channel photomultiplier tube (realplex4)9 orders of magnitude from starting copy number 50 fM fluorescein96 x 0,2 ml PCR tubes or one 96 PCR plate(unskirted, semi-skirted, skirted-as per SBS standard)4-99 C1-20 C (thermomodule Mastercycler ep)1-24 C (thermomodule Mastercycler ep S)30-99 C105 C35 C 0,3 C90 C 0,4 C 0,2 Capprox. 4 C/s (thermomodule Mastercycler ep)approx. 6 C/s (thermomodule Mastercycler ep S)approx. 3 C/s (thermomodule Mastercycler ep)approx. 4,5 C/s (thermomodule Mastercycler ep S)26 cm x 41 cm x 39,6 cm24 kg17 kg7 kg200-240V, 50 Hz - 60 Hz800 W