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Welcome to UIS2 OpticsWelcome to UIS2 OpticsUIS2:The System That Maximizes the Advantage of Infinity-corrected OpticsWhat is Infinity-corrected Optics?light between the objective lens and tube lens,allowing the creation of user-specific or taskspecific optical systems. To establish real flexibilitywith such a system, it is necessary to eliminate theoccurrence of coma aberration.UIS2 optics is an infinity-corrected optical system— in other words, a system in which light passesfrom the specimen through the objective lenswithout forming an image along the way. Instead,it travels in the form of infinity parallel rays to thetube lens. The tube lens is where the intermediateimage is formed, whereas in finite-correctedoptics, this is done by the objective lens.*In UIS2 objective lenses, the parfocal distance is designed atFigure 2 Advantages of Infinity-correctedOptical SystemFinite-corrected Optical SystemFigure 1 Infinity-corrected andFinite-corrected Optical System PrinciplesInfinity-corrected Optical SystemUIS/UIS2Objective LensTube LensFinite-corrected Optical SystemObjectiveLens4. LighteningWeight has been reduced to approximately 2/3that of conventional products by using analuminum objective lens barrel cover. This hasthe effect of lightening the load on the devices atobjective lens up/down, suppressing vibrationsby lowering the inertial force at objective lensswitching, etc. (MPLFLN series, LMPLFLNseries)UIS2 objective lenses ensure compatibility (screwdiameter, optical performance) with the UIS opticalsystem and have the following features comparedto conventional objective lenses.45 mm and the focal length of the tube lens is 180 mm.Infinity-corrected Optical SystemObjectiveLensEyepieceParallel Light BeamFeatures of UIS2 Objective LensesObjectiveLensTube Lens1. Wavefront Aberration ControlThe Olympus UIS2 objective lenses set a newstandard, with wavefront aberration control inaddition to common performance standards ofN.A. and W.D. Olympus challenges fartherhighest order optics which has not been fulfilledby the conventional standards. We offerexcellent performance objective lenses byminimizing the aberrations that lower resolution.5. Adoption of Eco-lensThe glass materials of UIS2 objective lenses areall lead- and cadmium-free eco-glass.Based on our conviction that the UIS2 systemis the best way to maximize the advantagesof infinity-corrected optical systems, weconfidently recommend the UIS2-featuredOlympus microscope units for all your highprecision needs in research, inspection andproduction equipment.*Wave front aberration: refer to the optical terminology at theIntermediateImageBasic Dimensions of UIS2 Optical Systemend of this document.EyepieceThe UIS2 optical system optimally correctsaberration with a dedicated telan lens and aneyepiece so that the coma aberration and flatnessare not degraded even when the telan lens exitpupil position is changed by changing theobjective lens and telan distance. This makes itpossible to use a distance of 50 mm to 170 mmfrom objective lens mounting position to the singleport tube with lens.IntermediateImageAdvantages of Infinity-corrected OpticsThis system, known as "infinity-corrected optics",offers a number of advantages:*Coma aberration: refer to the optical terminology at the end of There is no change in magnification even whenthe distance between the objective lens and tubelens is altered. With the total magnification remaining constant,there is no image aberration — even whenprisms or sliders are interposed between theobjective lens and the tube lens.this document.Figure 3 Basic Dimensions of UIS2 Optical SystemU-TLU(Single Port Tube with Lens)Objective Lens* 40 mmImage* 84 mm45 mm Recommended Distance50–170 mmAs thousands of users have found by experience,these advantages are crucial to composing theideal microscope optical system. What's more, itis even possible to freely insert or removeintermediate attachments in the parallel rays of57.6 mm102 mm2. Objective Lenses with Excellent ImageParcentricityHigh power SemiApochromatic UIS2 objectivelenses make the centration tolerance betweenobjective lenses on the microscope nosepiecekeep the image within the enter of the field ofview even with digital cameras. (50x or higherpower in both MPLFLN and LMPLFLN series)* Refer to the Olympus home page for detailedobjective lenses specifications.3. Improvement of Color ReproducibilityUIS2 objective lenses realize natural colorreproduction without any chromatic shifts usingstringently selected high transmittance glassand advanced coating technology that provideshigh transmittance which is flat over an ultrawide band wavelength. In addition, since thetotal optical system, including the tube lens isdesigned to reproduce a natural color, clearimages faithful to the specimen are obtainedeven with digital imaging.*Basic dimensions when our revolving nosepiece and illuminator arecombined. When the position of the illuminator above is changed,illumination performance cannot be maintained.34

System DiagramSystem DiagramBXFM System DiagramMotorized Unit System Diagram(refer to pages 40-44)Video System(refer to pages 38-39)Video CameraVideo CameraC-mountB Mount 2/3"Video CameraS Mount 2/3"Video CameraF MountObservation Tubes(refer to pages 30-31)U-AN(refer to pages ate Tubes(refer to pages or -FWRObservation/Single Tubes and Eyepieces(refer to pages 31-32, 35)WHNEyepiecesSWHEyepiecesWidefield TrinocularObservation TubesSuper Widefield TrinocularObservation TubesTH4-HSU-TLUU-TLUIRU-D6REMCU-PO3U-POIR(refer to page 37)(refer to pages 7-19)BF Objective LensesU-DICRHIllumination Systems and Power Supply(refer to pages 24-30)U-ANTH4-100U-ZPCBZ -RLAABX-RFAABX-UCB*U-HSTR2BD-M-ADU-25ND6, U-25ND25,U-25LBD, U-25IF550,U-25L42, U-25FR,U-BP1100IR, U-BP1200IRU-POTP3Revolving Nosepiece(refer to pages 36-37)U-D5BDREMCU-D6BDREMCU-DICRHCBF/DF Objective LensesPCSee manualanalySIS FIVEControl Software* BX-REMCB is also available for BX-RLAA motorized revolving nosepiece control (refer to page BX-KMA-ESDBXFM-A System DiagramSZX-TLGAD(refer to page 44)U-RCVLG-SFLG-PS2(refer to page 27)ø32 FilterVideo System(refer to pages 38-39)U-LGADFocusing Units(refer to pages 20-23)Auxiliary Lens(provided with the BXFMA-F)Observation Tubes(refer to pages 5XEAPOU-AN(refer to page refer to page 28)BXFMA-FStands(refer to page 23)U-FWRU-LH100-3U-LH100L-3U-RMTBX-UCBU-ZPCBZ BoardSee MCObjective ent types may be offered in each area.56analySIS FIVEControl Software

UIS2 Objective LensesUIS2 Objective Lenses Meaning of Abbreviations Shown on Objective LensM Plan ApochromatMetallurgical (no cover)Long Working DistanceMetallurgical UseSLM: Super Long WorkingDistance Metallurgical UseLC: Observation ThroughSubstratePL: Plan/Corrects FieldCurvature ofthe Periphery ofthe Image PlaneNone: Achromat/Corrects Aberration at 2 Wavelengths of Blue and RedFL:SemiApochromat/Corrects Chromatic Aberration in the Visible Range(violet red)APO: Apochromat/Optimally Corrects Chromatic Aberration in the EntireVisible Band (violet red)MPLAPON SeriesNumber:Objective LensMagnificationNone: BrightfieldBD: Brightfield/DarkfieldBDP: Brightfield/Darkfield/PolarizingIR:IRLCD: LCDPlan Apochromat objective lenses that correct chromatic aberrations at the highest level. Olympus guarantees* opticalperformance (wavefront aberration) with a Strehl ratio** of 95% or better. They are also designed for use with Olympus'U-AFA2M active AF unit.MPLAPON50xMPLAPON100xø20.32ø20.324.5M:LM:B D4.5M P L (Plan) F L N - 1 0 0* Definition of guaranteed values: Measurementsassessed with Olympus’ Interferometer forTransmitted Wavefront Measurement under specifiedconditions (measurement: temp. 23 C 1 C;assessment: measurement within the 97% range ofthe pupil dia.).** Strehl ratio: Indicates in percent (%) the ratio of theproportion of light that an actual optical system canconcentrate with respect to the proportion of lightconcentrated in the image plane (central intensity) byan ideal, aberration-free optical system, with the latterserving as 100%. A higher percentage indicates ahigher quality optical system.38.4342.442.784145For Brightfield Observation(44.65)Cover Glass Thickness (no cover)37.9845Objective Lenses Series Abbreviation (PL: Plan)N.A. (Numerical Aperture)(44.65)MagnificationInfinity-corrected Optical System41.62 Objective Lens NotationField Numberø8.8F.N. (Field Number)26.526.51.25x: 22 /2.5x: 26.5\U\L\\\*3\\L\UaU\L\a\\*3\*3\ø20.3ø28.5Use together with polarizer andanalyzer recommended.for near-IR observationfor near-IR observation*1 DIC Prism U-DICR: UM/LM Position, U-DICRHC: LM Position Fixed, U-DICRH: UM Position Fixed. *2 40x: BF Only *3 5–20x: U Excitation Also Possible\: Responds a: Optimally Responds BF: Brightfield DF: Darkfield DIC: Differential Interference Contrast POL: Polarized light FL: FluorescenceMPLAPON 50xMPLAPON 100xNumericalApertureWidefield Eyepiece WHN10xField Number 22Working Distance Focal Distancef (mm)(mm)0.950.95Unit: mmø28UIS2 Objective LensesObjective Lens(magnification)for LCDø16ø20.20.350.353.61.8Weight(g)139125Super Widefield Eyepiece SWH10xField Number 26.5TotalPractical Field Depth ofTotalPractical Field Depth ofMagnifications of View (mm) Focus (µm) Magnifications of View (mm) Focus rew: W20.32x0.706 (0.8"x1/36")M Plan ApochromatMPLAPON 100xO Features of Objective Lens Seriesa MPLAPON Series: M Plan Apochromat — P 8Plan Apochromat objective lenses that correct chromatic aberrations at the highest level.Olympus guarantees* optical performance (wavefront aberration) with a Strehl ratio** of 95%or better. They are also designed for use with Olympus' U-AFA2M active AF unit.a LCPLN-IR Series: IR Long WD M Plan Achromat — P 15Exclusive for the near-infrared microscopy largely of the internal structure in silicon wafers,LCPLN-IR series has correction collar for aberration dependent on thickness of silicon orglass substrate.a MPLFLN-BD Series: M Plan SemiApochromat BD — P 16Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration.The series secures a W.D. of 1 mm or longer. Since the exit pupil position of the 5x-150xobjective lenses is standardized, the position of the DIC prism does not have to be switchedwhen changing the magnification.a MPLFLN-BDP Series: M Plan SemiApochromat BDP — P 17Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration.The series secures a W.D. of 1 mm or longer. Since the exit pupil position of the 5x-100xobjective lenses is standardized, the position of the DIC prism does not have to be switchedwhen changing the magnification. The BDP series optimizing brightfield/darkfield andpolarized light characteristics is perfect for Nomarski DIC and polarized light observations.a LMPLFLN-BD Series: Long WD M Plan SemiApochromat BD — P 18Long working distance Plan SemiApochromat objective lenses, giving high-level correctionfor chromatic aberration. Suitable with samples having a height difference and in preventingcollision, as the working distance is long. Also, since the exit pupil position of the 5x-100xobjective lenses is standardized, the position of the DIC prism does not have to be switchedwhen changing the magnification.a MPLN-BD Series: M Plan Achromat BD — P 19Plan Achromat objective lenses providing excellent image flatness up to F.N. 22.* Definition of guaranteed values: Measurements assessed with Olympus’ Interferometer forTransmitted Wavefront Measurement under specified conditions (measurement: temp.23 C 1 C; assessment: measurement within the 97% range of the pupil dia.).** Strehl ratio: Indicates in percent (%) the ratio of the proportion of light that an actualoptical system can concentrate with respect to the proportion of light concentrated in theimage plane (central intensity) by an ideal, aberration-free optical system, with the latterserving as 100%. A higher percentage indicates a higher quality optical system.*** Specified oil: IMMOIL-F30CCa MPLAPON 100xO: M Plan Apochromat — P 8This is a Plan Apochromat objective lens of the oil-immersion type*** that features anumerical aperture of 1.4. It provides the highest level of chromatic aberration correctionand a high resolving power.a MPLFLN Series: M Plan SemiApochromat — P 9Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration.The lineup consists of 8 objective lenses ranging from 1.25x to 100x, and secures a W.D. of1 mm or longer (except 40x). Since the exit pupil position of the 5x-100x objective lenses isstandardized, the position of the DIC prism does not have to be switched when changing themagnification (40x is not applicable to DIC observation). For ultra low magnifications (1.25x,2.5x), use together with analyzer and polarizer of the reflected light illuminator.a LMPLFLN Series: Long WD M Plan SemiApochromat — P 10Long working distance Plan SemiApochromat objective lenses, giving high-level correctionfor chromatic aberration. Suitable with samples having a height difference and in preventingcollision, as the working distance is long. Also, since the exit pupil position of the 5x-100xobjective lenses is standardized, the position of the DIC prism does not have to be switchedwhen changing the magnification.a MPLN Series: M Plan Achromat — P 11Plan Achromat objective lenses providing excellent image flatness up to F.N. 22.a LCPLFLN-LCD Series: LCD Long WD M Plan SemiApochromat — P 12Perfect objective lens series for observation of LCD panels and other samples through aglass substrate. Aberration correction matched to the glass thickness is accomplished usinga correction collar.a SLMPLN Series: Super Long WD M Plan Achromat — P 13Plan Achromat objective lenses with high magnification and super long working distance.Three magnifications, 20x, 50x and 100x are available. For 5x or 10x objective lenses,select from the LMPLFLN Series.a LMPLN-IR Series: IR Long WD M Plan Achromat — P 14Objective lens series exclusive for the near-infrared microscopy largely of the internalstructure in silicon wafers.This is a Plan Apochromat objective lens of the oil-immersion type*** that features a numerical aperture of 1.4. It provides thehighest level of chromatic aberration correction and a high resolving /10/20/50/1005/10/20/50/1005/10/20/50/100POL\W.D. MPLN-BDBF\\(44.9)MPLAPONMPLAPON OMPLFLNMagnification50/100100*** Specified Oil: IMMOIL-F30CCø6.6ø17.5W.D. 0.1Seriesø5.5ø18.9W.D. 0.35 Objective Lens Series Listø22ø30Unit: mmWidefield Eyepiece WHN10xField Number 22UIS2 Objective LensesObjective Lens(magnification)MPLAPON 100xONumericalAperture1.4Working Distance Focal Distancef (mm)(mm)0.11.8Weight(g)1587TotalPractical Field Depth ofTotalPractical Field Depth ofMagnifications of View (mm) Focus (µm) Magnifications of View (mm) Focus (µm)1000Screw: W20.32x0.706 (0.8"x1/36")8Super Widefield Eyepiece SWH10xField Number 26.50.220.5910000.270.59

UIS2 Objective LensesUIS2 Objective LensesM Plan SemiApochromatLong WD M Plan SemiApochromatMPLFLN SeriesLMPLFLN SeriesPlan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The lineup consists of 8 objective lensesranging from 1.25x to 100x, and secures a W.D. of 1 mm or longer (except 40x). Since the exit pupil position of the 5x–100x objectivelenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification (40x is not applicableto DIC observation). For ultra low magnifications (1.25x, 2.5x), use together with analyzer and polarizer of the reflected light illuminator.Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. Suitablewith samples having a height difference and in preventing collision, as the working distance is long. Also, since the exit pupilposition of the 5x–100x objective lenses is standardized, the position of the DIC prism does not have to be switched whenchanging the 45(34.4)ø15.2W.D. 12W.D. 21W.D. 22.5ø20.32W.D. 10.6ø15.2ø25.8ø20.324538.442.442.61ø7.8W.D. . 8.5W.D. 0.63(44.37)35.1W.D. .D. �25.430.4ø30W.D. .D. 20ø304.54.531.6ø24.5W.D. .D. 1.25x*(W.D.: Working Distance)ø26Unit: mmWidefield Eyepiece WHN10xField Number 22UIS2 Objective LensesObjective Lens(magnification)NumericalApertureMPLFLN 1.25x0.04MPLFLN 2.5x0.08MPLFLN 5x0.15MPLFLN 10x0.30MPLFLN 20x0.45MPLFLN 40x0.75MPLFLN 50x0.80MPLFLN 100x0.90Screw: W20.32x0.706 (0.8"x1/36")Working Distance Focal Distancef .61.8Weight(g)12210651.568.170.412089.990.9Unit: mmSuper widefield Eyepiece SWH10xField Number 26.5TotalPractical Field Depth ofTotalPractical Field Depth ofMagnifications of View (mm) Focus (µm) Magnifications of View (mm) Focus .30.73Widefield Eyepiece WHN10xField Number 22UIS2 Objective LensesObjective 500.80Working Distance Focal Distancef 47377949TotalPractical Field Depth ofTotalPractical Field Depth ofMagnifications of View (mm) Focus (µm) Magnifications of View (mm) Focus (µm)501002005001000Screw: W20.32x0.706 (0.8"x1/36")10Super Widefield Eyepiece SWH10xField Number 5.32.71.30.530.2770186.12.50.87

UIS2 Objective LensesUIS2 Objective LensesM Plan AchromatLCD Long WD M Plan SemiApochromatMPLN SeriesLCPLFLN-LCD SeriesPlan Achromat objective lenses providing excellent image flatness up to F.N. 22.Perfect objective lens series for observation of LCD panels and other samples through a glass substrate. Aberration correctionmatched to the glass thickness is accomplished using a correction 20.32ø24ø312027.5ø15.2ø25t 0.741.643.445.238 *t 0.7ø25(43.638)2538.6540.45204.8(42.038)45.238 *24.534.7520ø29.5ø17.84W.D. 0.9ø24ø25ø12.77W.D. 2.5ø15.8ø15t 0.7ø11.9ø1636.554.5W.D. 7.8ø6(36.738)45.238 *37.241.342.445ø12W.D. 1.3ø24(43.7)28.833.632.714.5ø10.5W.D. 10.6(34.4)4523.44.5(25)45W.D. 20ø214.5MPLN10x4.5MPLN5xø29.5ø31ø29.5Unit: mmø31* Value at glass thickness 0.7 mm observation.MPLN100xø20.32ø20.32Objective LensCorresponding Glass Thickness (mm)Correction Collar IndicationWorking Distance (mm)Correction Systemø15.8W.D. 0.21W.D. 0.38ø11.91.27.403.0NumericalApertureLCPLFLN 20xLCD0.45LCPLFLN 50xLCD0.70LCPLFLN 100xLCD0.85Screw: W20.32x0.706 (0.8"x1/36")ø4.4ø11.6ø15.6Working Distance** Focal Distancef (mm)(mm)Unit: mmWidefield Eyepiece WHN10xField Number 22UIS2 Objective LensesObjective 100xNumericalAperture0.100.250.400.750.90Working 0xLCD0–0.70.50.98Correction Collar0.70.9Focal Distancef l Field of Depth of FocusMagnificationsView 20.73Screw: W20.32x0.706 (0.8"x1/36")11Super widefield Eyepiece SWH10xField Number 26.5TotalPractical Field Depth ofTotalPractical Field Depth ofWeightMagnifications of View (mm) Focus (µm) Magnificat

The wide range of Olympus components introduced here allows users in such diverse fields as research, inspection and production to take advantage of the quality, flexibility and outstanding optical performance of the UIS2 Optical System. That's why installing Olympus microscope components is, quite simply, the right choice for your equipment. 1