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IntelliCyt iQue Screener PLUSFluorochrome Chart
Masterful Panel Design Reveals InsightDeveloping a high content and physiologically relevant multicolor panel is similar to the creation ofan artistic masterpiece. The interplay of complex science and perfectly selected fluorochromes resolveunanswered questions in cellular research by multiplexing markers of cell phenotype and function.Combining cell and bead-based reagents together in the same well allows researchers to efficientlycharacterize disease states.Designing Multicolor Experiments is as Easy as 1, 2, 3:123Know your instrumentKnow your detection reagentsKnow your biology What are the number and type of lasersavailable? What laser wavelength excites them? Reserve the brightest fluorochromes forthe lowest expressed anitgens and viceversa. What is the emission spectra? What detector/filter combinations areavailable to capture the signal?This determines what labeling and detectionreagents are possible to use on your systemand how they will perform. Will there be a lot of spillover betweenadjacent channels? How bright are they? What is their stainindex? Beware that tandems and other dyescan degrade when exposed to light,temperature changes, or fixation.This information will allow you to determinewhich flurochrome will be best matched towhich antigen or marker of interest. Avoid spillover of highly expressedantigens into detectors requiring highsensitivity.Now, you have enough information to beginto answer your experimental questions byoptimizing fluorochromes and dyes for yourbiology on your instrument.
IntelliCyt iQue Screener PLUS and iQue Screener PLUS HDBlue-Red ConfigurationMultiCyt reagents are indicated in Blue.LaserExcitationiQue ScreenerPLUS rescentFluorochromes ProteinsFITCeGFPAlexa Fluor 488eYFPHorizon BB515EmeraldmNeon GreenViabilityFL1 Cell MembraneIntegrity DyeCalcein AMLIVE/DEAD FixableGreenZombie Green572/28BL2PEPIeFluor 570Proliferation,Function,and ApoptosisFL1 CellProliferation andEncoder DyeCaspase 3/7DNA and CellCycleVybrant Dye CycleGreenClick-iT Plus EdUAlexa Fluor 488MicronucleusInduction AssayAnnexin VPIQBeads PlexScreenBlue488 nmSYTOX Orange675/30BL4PerCP-Cy 5, Cy5.5PE-Cy5, Cy5.5eFluor PE-AlexaFluor 647FL3 Cell MembraneIntegrity DyeCell Cycle DyePI7AADPIPerCp-Vio700 780/60BL5PE-Cy7PE-Vio770 675/30RL1APCAlexa Fluor 647APC-Alexa Fluor 680FL4 Cell MembraneIntegrity DyeFVD eFluor 660LIVE/DEAD FixableFar RedRed640 nm780/60RL2FL4 CellProliferation andEncoder DyeQBeads PlexScreenClick-iT Plus EdUAlexa Fluor 647FxCycle Far RedQBeads DevScreenSH, sAVAPC-Alexa Fluor 750FVD eFluor 780QBeads PlexScreenAPC-Cy7LIVE/DEAD FixableNear IRQBeads DevScreenSH, sAVAPC-Vio770DRAQ 5Vybrant DyeCycleRuby
IntelliCyt iQue Screener PLUSViolet-Blue-Red ConfigurationLaserExcitationMultiCyt reagents are indicated in Blue.iQue ScreenerPLUS uorochromes Proteins445/45Horizon nd ApoptosisDNA and CellCycleeBFPCalcein VioletCellTrace VioletDAPIPacific BlueAzuriteSYTOX BlueFXCycle VioletAlexa Fluor 405CeruleanLIVE/DEAD FixableVioletClick-iT Plus EdUPacific BluePacific GreenvGFPFL1 CellProliferation andEncoder DyeVybrant Dye CycleGreenHorizon BV510Vybrant DyeCycleVioletDAPILIVE/DEAD FixableAquaVioGreen Violet405 nm572/28VL3Horizon BV570LIVE/DEAD FixableYellowQdot 605615/24VL4Horizon BV605Qdot 625675/30VL5Horizon BV650Qdot 655780/60VL6Horizon BV785530/30BL1FITCeGFPAlexa Fluor 488eYFPHorizon BB515EmeraldmNeon Green572/28BL2PEFL1 Cell MembraneIntegrity DyeCalcein AMLIVE/DEAD FixableGreenZombie GreenPIeFluor 570Caspase 3/7Click-iT Plus EdUAlexa Fluor 488MicronucleusInduction AssayAnnexin VPIQBeads PlexScreenSYTOX OrangeBlue488 nm615/24BL3Horizon PE-CF594PE/Dazzle 594PE-Texas Red675/30BL4PE-Alexa Fluor 610PerCP-Cy5, Cy5.57AADPE-Cy5, Cy5.5PIFL3 Cell MembraneIntegrity DyeCell Cycle DyePIeFluor PE-AlexaFluor CAlexa Fluor 647APC-Alexa Fluor 680FL4 Cell MembraneIntegrity DyeFVD eFluor 660LIVE/DEAD FixableFar RedRed640 nm780/60RL2FL4 CellProliferation andEncoder DyeQBeads PlexScreenAPC-Alexa Fluor 750FVD eFluor 780QBeads DevScreenSH, sAVQBeads PlexScreenAPC-Cy7LIVE/DEAD FixableNear IRQBeads DevScreenSH, sAVAPC-Vio770DRAQ5Click-iT Plus EdUAlexa Fluor 647FxCycle Far RedVybrant DyeCycleRuby
IntelliCyt iQue Screener PLUSViolet-Yellow-Blue ConfigurationLaserExcitationiQue ScreenerPLUS uorochromes Proteins445/45Horizon BV421VL1MultiCyt reagents are indicated in Blue.ViabilityProliferation,Function,and ApoptosisDNA and CellCycleAzuriteCalcein VioletCell Trace VioletDAPIPacific BlueCeruleanSYTOX BlueFXCycle VioletHorizon V450eBFPLIVE/DEAD FixableVioletClick-iT Plus EdUPacific BlueAnnexin VVybrant DyeCycleOrangeAlexa Fluor 405Vybrant DyeCycleVioletDAPIViolet405 /20YL1Pacific GreenvGFPHorizon BV510Horizon BV570LIVE/DEAD FixableAquaLIVE/DEAD FixableYellowQdot 605Horizon BV605Qdot 625Horizon BV650Qdot 655Horizon BV785PEmStrawberryAlexa Fluor 546tdTomatoAsRed 2LIVE/DEAD FixableRedCellROX OrangeSYTOX OrangemRFP615/20YL2Yellow561 nm660/20YL3CellTrace CalceinRed-Orange AMHorizon PE-CF594mCherryCell Tracker OrangeSYTOX AADvanced PE/Dazzle 594HcRedCell Tracker RedDRAQ7PE-Texas RedmRubyPE-Alexa Fluor 610mStrawberryPE-Cy5, Cy5.5mPlumDRAQ5eFluor PE- Alexa Fluor647780/60YL4Click-iT Plus EdUAlexa Fluor 594Vybrant DyeCycleRubyPE-Cy7PE-Alexa 750PE-Vio770530/30BL1Horizon BB515eGFPFITCeYFPAlexa Fluor 488EmeraldmNeon GreenFL1 Cell MembraneIntegrity DyeCalcein AMLIVE/DEAD FixableGreenZombie GreenBlue488 nm615/20BL3FL1 CellProliferation andEncoder DyeCaspase 3/7Vybrant Dye CycleGreenClick-iT Plus EduAlexa Fluor 488MicronucleusInduction AssayHorizon PE-CF594PE/Dazzle 594PE-Texas RedPE-Alexa Fluor 610660/20BL4PerCPPerCp-Cy5, Cy5.5PE-Cy5, Cy5.5PerCp-Vio700FL3 Cell MembraneIntegrity Dye7AADPICell Cycle DyePI
IntelliCyt iQue ScreenerLaserExcitationMultiCyt reagents are indicated in Blue.iQue ScreenerPLUS uorochromes Proteins530/30Horizon BB515eGFPFITCeYFPAlexa Fluor 488EmeraldFL1mNeon Green585/40FL2PEViabilityFL1 Cell MembraneIntegrity DyeCalcein AMLIVE/DEAD FixableGreenPIProliferation,Function,and ApoptosisFL1 CellProliferation andEncoder DyeDNA and CellCycleClick-iT Plus EdUAlexa Fluor 488Caspase 3/7MicronucleusInduction AssayAnnexin VPIQBeads PlexScreenSYTOX OrangeBlue488 nm670LPFL3Used in 3:1config.PerCPPerCp-Cy5, Cy5.5PE-Cy5, Cy5.5, Cy7PerCp-Vio700780/60(optional filter)FL3 (SDS)PE-Cy7Used in 4:0config.PE-Vio770675/25(optional filter)FL4 (SDS)PerCPUsed in 4.0config.PerCp-Cy5,Cy5.5PE-Cy5, Cy5.5PerCp-Vio700675/30FL4APCAlexa Fluor 647APC-Alexa Fluor 680FL3 Cell MembraneIntegrity Dye780/60PIPI7AADFL3 Cell MembraneIntegrity DyeCell Cycle DyePIPI7AADFL4 Cell MembraneIntegrity DyeFVD eFluor 660LIVE/DEAD FixableFar Red.Red640 nmCell Cycle DyeFL4 CellProliferation andEncoder DyeClick-iT Plus EdUAlexa Fluor 647QBeads PlexScreenQBeads DevScreensAV, SHFL3 (SDS)APC-Alexa Fluor 750FVD eFluor 780QBeads PlexScreenUsed in 2:2config.APC-Cy7LIVE/DEAD FixableNear IRQBeads DevScreensAV, SHAPC-Vio770DRAQ5Learn why lntelliCyt is the choice of leaders inlmmuno-Oncology, Antibody Discovery andImmune Target Screeningwww.intellicyt.comFor more information or a quote, call us at 1 505-345-9075or send an email to sales@IntelliCyt.com 2017 IntelliCyt Corporation. All Rights Reserved.The trademarks used herein are the property of IntelliCyt Corporation or their respective owners.IntelliCyt technology is protected by the following patents and other patents pending:6,890,487, 6,878,556, 7,368,084, 7,842,244, 8,021,872, 8,268,571, 8,637,261, 8,823,943, 9,012,235, D,722,515Specifications subject to change without notice. Doc Number 12088 Rev D.FOR RESEARCH USE ONLY. NOT FOR IN VITRO DIAGNOSTIC USE.
Masterful Panel Design Reveals Insight Developing a high content and physiologically relevant multicolor panel is similar to the creation of an artistic masterpiece.
