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Axygen AxyPrep Magnetic BeadPurification KitsA Corning Brand
Sample Prep Solutions for GenomicsObtaining Pure Nucleic Acids from Your Sample is PreciousThe purification of high quality DNA/RNA is the first essential step for any genomics-baseddownstream application. Poor quality may contribute significantly to the failure of downstream applications and thus delay research and increase overall research costs.Although certain biological samples are not the limiting factor, there are samples that are verydifficult to obtain, which makes them precious. There is little room for error when processingthese precious samples, so utilizing a high quality nucleic acid purification technology becomesessential.Corning has developed a comprehensive range of magnetic beads-based isolation reagents.The Axygen AxyPrep Magnetic (MAG) Bead Purification Kits utilize a unique paramagneticbead-based chemistry for the purification and clean-up of nucleic acids for many genomicsdownstream applications such as DNA sequencing, genotyping, and gene expression. Free ofmajor hazardous chemicals, our products do not compromise on performance featuring highquality, high yields, excellent sample concentration, and long sequencing reads. Additionally,this makes our MAG kits easy to transport and store.Axygen IMAG Magnetic Separation Devices (MSD) and AxyPrep Magnetic Bead PurificationKits cut the total separation process and labor by up to 50%. IMAG MSDs are available in tube(IMAG-12T) or 96 well microplate format (IMAG-96P). They enable manual users to process anymagnetic beads-based purification or clean-up process as easily and conveniently as automation users for use in biological research, including nucleic acid purification and clean-up, cellbased assays, and antibody and protein purification.Our kits have been adopted by life science research institutes worldwide and continue to generate satisfaction and compliments from our customers for their excellent quality and performance.For a demonstration, contact your local Corning Life Sciences Account Manager.2
Axygen AxyPrep MAG Plant Genomic DNA Extraction KitThe AxyPrep MAG Plant gDNA Extraction Kit is a magnetic beads-based purification systemthat allows rapid and reliable isolation of high-quality gDNA from a wide variety of plant samples. Up to 96 samples (50 mg fresh tissue per sample or 15 mg of dried tissue per sample)can be processed in less than one hour. The AxyPrep MAG Plant gDNA Extraction Kit efficientlyeliminates polysaccharides, phenolic compounds and enzyme inhibitors with no organicextractions. This kit is adaptable for use on major liquid handling workstations for highthroughput processing.PCRwSanger and Next GenerationsequencingwFragment analysiswGenotyping and SNP detectionwRestriction enzyme clean-upwNon-alcohol-based purificationwCompatible with genomics downstream applicationswHigh-quality, high-yield DNAwManual and automation friendly YHUDJH 0HDVXUHPHQWV IRU J'1 ([WUDFWHG IURP &DQROD 6HHGV E\ 9DULRXV &RPPHUFLDO 3ODQW J'1 ([WUDFWLRQ .LWV wSimplified extraction process Comparison of DNAextracted using AxygenAxyPrep MAG Plant gDNAKit versus Company P’sequivalent kit. Using liquidN2, canola seeds wereground to fine powder.Twenty milligrams wereweighed into a 1.5 mL tubefor extraction. Quality wasmeasured with A260/280(top). Yields was measuredwith Picogreen (bottom). &RPSHWLWRU &RPSHWLWRU 0 &RPSHWLWRU 3 [\3UHS 0 * 3ODQW YHUDJH J'1 LHOG ([WUDFWHG IURP &DQROD 6HHGV E\ 9DULRXV &RPPHUFLDO 3ODQW J'1 ([WUDFWLRQ .LWV &RQFHQWUDWLRQ QJ /Applicationsw &RPSHWLWRU &RPSHWLWRU 0 &RPSHWLWRU 3 [\3UHS 0 * 3ODQWMagnetic Beads KitsKit SizeCat. No.AxyPrep MAG Plant gDNA Extraction Kit, 10 mL10 prepsMAG-PLANT-GDNA-10AxyPrep MAG Plant gDNA Extraction Kit, Small96 prepsMAG-PLANT-GDNA-SAxyPrep MAG Plant gDNA Extraction Kit , Medium384 prepsMAG-PLANT-GDNA-M3
Axygen AxyPrep MAG Viral Nucleic Acid Purification KitThe AxyPrep MAG Viral Nucleic Acid Purification Kit utilizes a unique paramagnetic beadsbased purification system for the purification of viral nucleic acid from 200 μL of body fluid,including plasma, serum, ascites, cell culture supernatant, cerebrospinal fluid, urine and otherbody fluids.ApplicationswPCRwSanger and Next GenerationsequencingwGenotyping and SNP detectionwGene expressionwSimplified extraction processwCompatible with genomics downstream applicationswHigh-quality, high-yield nucleic acid purificationwManual and automation friendlyPurified viral RNA can be successfully amplifiedin Real-time(RT)-PCRHAV vaccine RNA was purified from 200 μLserum-diluted HAV vaccine with AxyPrep MAGViral Nucleic Acid Purification Kit. Viral RNA waseluted in 50 μL nuclease free water. 5 μL templatewas added into a 20 μL RT-PCR reaction mix. Eachlane was loaded with 5 μL RT-PCR product.Purified viral RNA can be successfully amplifiedin RT-qPCRRT-qPCR detection of Hepatitus B virus was iso lated using the AxyPrep MAG Viral Nucleic AcidPurification Kit. A 10-fold dilution series of therecovered RNA was used in a SYBR Green-basedRT-qPCR reaction. Each reaction was per formedin triplicate.Mumps vaccine RNA was purified from 200 μLserum-diluted MMR vaccine with AxyPrep MAGViral Nucleic Acid Purification Kit. Purified nucleicacids was eluted in 50 μL nuclease-free water. 5 μLtemplate was added into a 20 μL RT-PCR reactionmix. Each lane was loaded with 5 μL RT-PCR product.4Magnetic Beads KitsKit SizeCat. No.AxyPrep MAG Viral Nucleic Acid Purification Kit, Small96 prepsMAG-VNA-SAxyPrep MAG Viral Nucleic Acid Purification Kit, Medium384 prepsMAG-VNA-M
Axygen AxyPrep MAG DNA Concentrate KitThe AxyPrep MAG DNA Concentrate Kit utilizes a paramagnetic beads-based procedure to purify and concentrate DNA from genomic DNA and plasmids. The kit efficiently removes all contaminants such as salts, proteins, enzymes, nucleotides, and primers while concentrating theDNA. The kit is suitable for concentrating DNA from samples, especially from those with lowconcentration of DNA such as microdissection and paraffin-embedded tissue or DNA reactionssuch as restriction enzyme digestion, PCR reactions and minipreps. The kit can also be used toreduce the volume required in library preparation for NGS platforms.AxyPrep MAG DNA Concentrate Kit is designed for both manual and fully automated purificationof sequencing products. It can be used with most automated liquid handling workstations.ApplicationswSanger and Next GenerationsequencingwDNA extraction recoverywGenotyping and SNP detectionwForensicswSimplified DNA concentration processwHigh recovery of high quality DNAwCompatible with genomics downstream applicationswManual and automation friendlyDouble the recovery in halfthe volume: Various DNAladder (50 bp) volumes wereprocessed with the AxyPrepMAG DNA Concentrate Kit,then eluted in 5 µL andloaded on 1.2% agarose gel.The gel image shows thatinput DNA ladder sampleswere concentrated withoutrecovery loss.Magnetic Beads KitsKit SizeCat. No.AxyPrep MAG DNA Concentrate Kit, 10 Preps10 prepsMAG-CONDNA-10AxyPrep MAG DNA Concentrate Kit, Small96 prepsMAG-CONDNA-SAxyPrep MAG DNA Concentrate Kit, Medium384 prepsMAG-CONDNA-M5
Axygen AxyPrep MAG Soil, Stool, and Water DNA KitThe AxyPrep MAG Soil, Stool, and Water DNA Kit utilizes a paramagnetic beads-based procedure.Soil, stool and water samples typically contain many compounds that can degrade DNA andinhibit downstream enzymatic reactions. The kit uses a unique buffer system that can removeinhibitory substances from these samples.The kit allows rapid and reliable isolation of high quality total DNA from fresh and frozen stoolsamples. Up to 500 mg stool samples can be processed in less than 60 minutes. The systemcombines the reversible nucleic acid binding properties of our proprietary chemistry with highefficiency, eliminating the carryover of humic acid, polysaccharides, phenolic compounds, andenzyme inhibitors from soil, stool, and contaminated water samples. Purified DNA is suitablefor PCR, restriction digestion, and hybridization techniques. There are no organic extractions,thus reducing plastic waste and hands-on time allowing multiple samples to be processed inparallel.ApplicationswPCR and QPCRwSanger and Next GenerationsequencingwGenotyping and SNP detectionwRestriction digestionwSimplified DNA extraction process: 96 well microplate samples in less than an hourwInhibitor-free DNA purification processwCompatible with genomics downstream applicationswManual and automation friendlyHigh Yields and High Quality DNAAB(A) Graph illustrating the average yield and A260/280 obtained from purifying 8 stool samples of 500 mg inputeach. (B) Gel image of the 8 samples.No Inhibitors Carry Over in PCRNo inhibition observedpost PCR. The fungal ITSand bacterial 16S rRNAgenes were utilized,10 µL loaded on a 1%Agarose gel.6Magnetic Beads KitsKit SizeCat. No.AxyPrep MAG Soil, Stool and Water DNA Kit, Small96 prepsMAG-STL-SAxyPrep MAG Soil, Stool and Water DNA Kit, Medium384 prepsMAG-STL-M
Axygen AxyPrep MAG PCR Clean-Up KitThe AxyPrep MAG PCR Clean-Up Kit is designed for efficient removal of unincorporated dNTPs,salts, and enzymes after any post PCR. These contaminants are detrimental to the success ofdownstream applications such as genotyping Sanger and next generation sequencing. TheAxyPrep MAG PCR Clean-Up Kit utilizes a unique paramagnetic bead technology for quick high-throughput purification of PCR amplicons. AxyPrep MAG PCR Clean-Up utilizes an optimized buffer to selectively bind PCR amplicons of 60-100 bp or 100 bp to paramagneticbeads, providing end users flexibility and high recovery options with efficient primer-dimerremoval based on their downstream application needs. The protocol mainly consists of binding, washing, and elution steps. Primers, nucleotides, salts and enzymes in PCR mixture areremoved during the binding and washing steps. The purified PCR product is essentially freeof contaminants.ApplicationswSanger sequencingwNext Generation sequencingwFlexible Clean-Up: High recovery of amplicons with the option to choose recovery of 60 bp or 100 bpwEfficient removal of unincorporated dNTPs, primers, primer dimers, salts, and other contaminantswManual and automation-compatible protocolwProcessing time: 15 minutes/96 sampleswNo centrifugation or filtration requiredAxyPrepAMPure XPMinEluteEfficient Removal ofPrimer DimersEliminates extra clean-up stepsand reduces the potential ofsequencing bias. Gel imagesof post clean-up of an Illuminalibrary post adaptor ligation.The white box demonstratesthat AxyPrep MAG Clean-Upresults in no primer dimer carryover which can be observedwith Agencourt AMPure and Qiagen MinElute .Magnetic Beads KitsKit SizeCat. No.AxyPrep MAG PCR Clean-Up Kit, 5 mL227 prepsMAG-PCR-CL-5AxyPrep MAG PCR Clean-Up Kit, 50 mL2,777 prepsMAG-PCR-CL-50AxyPrep MAG PCR Clean-Up Kit, 250 mL13,888 prepsMAG-PCR-CL-2507
Axygen AxyPrep MAG PCR Normalizer KitThe AxyPrep MAG PCR Normalizer Kit utilizes a paramagnetic beads-based purification systemfor post PCR clean-up. AxyPrep MAG PCR Normalizer beads have limited binding surface,therefore by limiting the amount of beads added in a given purification reaction, pre-definedamount of DNA can be isolated based on customers need. PCR normalization is accomplishedduring this purification process so that additional DNA quantification and dilution are notnecessary. Time, labor, and reagent cost are greatly saved with the AxyPrep MAG unique normalization purification chemistry, therefore efficiently streamlining genomics downstreamapplications. The protocol mainly consists of binding, washing, and elution steps, and it canbe performed directly in the thermal cycling plate and requires no centrifugation or filtration.The process can be automated with walk away solution for high throughput applications.ApplicationswSanger sequencingwNext Generation sequencingwDual action: Normalization and post PCR clean-up enables significant reduction inprocessing and faster turnaround timeswScalable: Tube, 96 and 384 well plate formatswManual and automation-compatible protocolwCompatible with several downstream applicationswNo centrifugation or filtration requiredDual action: Post PCR productnormalization and clean-up.Regardless of the post PCRproduct volume or concentrationadded to the AxyPrep MAG PCRNormalizer, the output would bea normalized PCR product to thedesired concentration withoutprimer dimer, free dNTPs, or saltscarry over.PCR Input (XYZ Volume/Conc.)PCR Output (X Conc)1234Gel image of various PCR productvolumes from a 50 µL PCRreaction treated with AxyPrepMAG PCR Normalizer. Regardlessof PCR input, a similar amount ofPCR Fragment is recovered.Lane 1Lane 2Lane 3Lane 485 µL PCR product10 µL PCR product15 µL PCR product20 µL PCR productMagnetic Beads KitsKit SizeCat. No.AxyPrep MAG PCR Normalizer Kit, 5 mL96 prepsMAG-PCR-NM-5AxyPrep MAG PCR Normalizer Kit, 50 mL384 prepsMAG-PCR-NM-50AxyPrep MAG PCR Normalizer Kit, 250 mL1920 prepsMAG-PCR-NM-250
Axygen AxyPrep MAG DyeClean Up KitExcess dye terminator clean-up is an essential step prior to sequencing. The carry over ofexcess dye into sequencing results in dye blobs, which leads to overwhelming noise andfailed calls of sequenced bases and potentially leading to the failure of the sequencing run.AxyPrep MAG DyeClean utilizes a unique paramagnetic bead-based sequencing purificationsystem optimized for the clean-up of excess dye terminator post sequencing reaction in Sangersequencing. The protocol mainly consists of binding, washing and elution steps, and can beperformed directly in the thermal cycling plate and requires no centrifugation or filtration.Sanger sequencingwHigh signal to noise ratio resulting in long reads and high QV20 scoreswSuitable for the clean-up of sequencing reaction of different Sanger sequencing based platforms – ABI Prism 3730, 3730xl, 3700. 3130, 31030xl, and 3100 – GE Healthcare MegaBACEwSimple and easy sequencing reaction clean-up process: 25 minutes/96 sampleswNo centrifugation or filtration requiredQV20 Scores Achieved Post Sequencing Reaction Clean-Up with AxyPrepMAG DyeCleanQV20 ScorewSaving time and money: Significant BigDye savings without incurring extra cost and processing time with the unneeded BigDye signal enhancer buffer, while maintaining high QV20 gDye DilutionQV20 Scores Achieved Post Sequencing Reaction Clean-Up with AxyPrepMAG DyeClean and BigDye Signal Enhancer BufferQV20 1216202428BigDye DilutionSavings Achieved On BigDye Without The Need For A Signal Enhancer While Maintaining High QV20 Scores.The QV20 scores achieved indicates that there is no need for the use of the BigDye Signal Enhancer buffer(MCLab) when AxyPrep MAG DyeClean is utilized for the post sequencing reaction clean-up. Sequencingreactions were performed with plasmid and primer provided in the BigDye v3.1 kit. The sequencing reactionscontained 250 ng of pGEM 3Zf( ) control plasmid, 3.2 pmol of M13(-21) primer and the indicated amount ofBigDye v3.1 in a final volume of 10 µL. The samples were processed on ABI 3730xl.Magnetic Beads KitsKit SizeCat. No.AxyPrep MAG PCR DyeClean Kit, 5 mL500 prepsMAG-DYECL-5AxyPrep MAG PCR DyeClean Kit, 50 mL5000 prepsMAG-DYECL-50AxyPrep MAG PCR DyeClean Kit, 250 mL25000 prepsMAG-DYECL-2509
Axygen AxyPrep MAG FragmentSelect-I KitPost DNA shearing, the library construction process for next generation sequencing mostlyrequires fragment selection regardless of the platform. Obtaining high recovery post fragment selection is becoming an important contributor for the reduction of sequencing bias.The AxyPrep MAG FragmentSelect-I Kit is optimized for Illumina next generation sequencingplatforms and Life Technologies (SOLiD ) fragment size selection needs in the Roche/454'sDNA Genome sequencer library construction workflow. The AxyPrep MAG FragmentSelect-I Kitutilizes a unique paramagnetic bead technology for quick high-throughput optimized DNA sizeselection suitable for various next generation sequencing platforms.ApplicationswIllumina Next GenerationsequencingwLife Technologies (SOLiD)f ragment size selectionwLibrary constructionwAxyPrep MAG FragmentSelect-I Kit optimized fragment selection chemistries for Illuminaand SOLiDwVersatile and flexible sequential fragment size selection: No gels neededwEfficient removal of primer-dimers: No need for further clean-up stepswManual and automation-compatiblewScalable: Tube, 96 and 384 well plate formatswNo centrifugation or filtration requiredInput1200 ngSheared DNA800 ng400 ng200 ng100 ng80 ngConsistently High Amplicon Recovery. The effect of concentration of input DNA on fragment selection measuredby Agilent BioAnalyzer on a High Sensitivity DNA chip.10Magnetic Beads KitsKit SizeCat. No.AxyPrep MAG FragmentSelect-I Kit, 5 mL4 prepsMAG-FRAG-I-5AxyPrep MAG FragmentSelect-I Kit, 50 mL380 prepsMAG-FRAG-I-50AxyPrep MAG FragmentSelect-I Kit, 250 mL1900 prepsMAG-FRAG-I-250
Axygen AxyPrep MAG Plasmid KitThe AxyPrep MAG Plasmid purification kit utilizes a magnetic beads-based technology forhigh-throughput purification of plasmid DNA from Escherichia coli cells. AxyPrep MAG PlasmidKit can also be used with fosmid and BAC vector-based constructs ranging from 5 Kb to 150 Kb.The system consists of steps of pelleting cells, resuspension, lysis, neutralization, DNA binding,ethanol washing, and plasmid elution. The protocol is automation-compatible.12345678910111213141516RNA contaminationcauses inaccurateplasmid yields. NoRNA contaminationis visible with theAxyPrep MAGPlasmid Kit whencompared to thecompetition.ApplicationswFluorescent DNA sequencingwTransformationwDNA sequencing (includingcapillary electrophoresiswPCR amplificationwRestriction enzyme digestionDifferent size plasmid purified with the AxyPrep MAG Plasmid Kit. 1% TBE agaroseGel run at 120 V for 12 minutes. 5 µL loaded on agarose gels. Elution volume 40 µL,Plasmid Size: 6KLane 1-8Axygen800 µL of 2xYTLane 9-16Competitor800 µL of 2xYTMagnetic Beads KitsKit SizeCat. No.AxyPrep MAG Plasmid Kit, Small96 prepsMAG-P-SAxyPrep MAG Plasmid Kit, Medium384 prepsMAG-P-M11
Axygen AxyPrep MAG Tissue-Blood gDNA KitOne Kit for Multiple Sample TypesThe AxyPrep MAG Tissue-Blood gDNA Kit utilizes a magnetic beads-based technology to isolate genomic DNA from rodent tail tissues. This protocol provides instruction to extract gDNAfrom fresh or frozen rodent tails. The protocol is performed in 96 well format and 1.5 mL tubes.Purification begins by the addition of lysis buffer, and Proteinase K to rupture cell membranesand digest protein. Genomic DNA is then immobilized on magnetic particles by the addition ofa magnetic binding reagent. This differential binding allows the gDNA to be easily separatedfrom contaminants using a magnetic field. Contaminants can then be rinsed away using asimple washing procedure.wExpanded protocol for animal tissue, cultured cells, buccal swabs, and bloodApplicationswRestriction enzyme digestionwDNA sequencingwGenotypingwPCR amplification12345678M910111213141516DNA tissue from chicken liver purified with the AxyPrep MAG Tissue and Blood gDNA Kit. 1% Gelrun at 130 V for 10 minutes. 5 µL DNA Solution used in each gel well. Elution volume 200 µLLane 1-8AxygenMAxygen 1 Kb DNA LadderLane 9-16Competitor1234510 mg of fresh chicken liver10 mg of fresh chicken liver678M910111213141516Human Whole Blood stored in EDTA purified with the AxyPrep MAG Tissue and Blood gDNA Kit. 1% Gelrun for 15 minutes. For the Competitor, 200 µL Elution Buffer used and 10 µL DNA solution used for eachwell. For Axygen, 100 µL Elution Buffer used, and 5 µL DNA solution used for each well.12Lane 1-8AxygenMAxygen 1 Kb DNA LadderLane 9-16Competitor200 µL Human whole blood stored in EDTA200 µL Human whole blood stored in EDTAMagnetic Beads KitsKit SizeCat. No.AxyPrep MAG Tissue -Blood gDNA Kit, Small96 prepsMAG-T-GDNA-SAxyPrep MAG Tissue - Blood gDNA Kit, Medium384 prepsMAG-T-GDNA-MAxyPrep MAG Tissue - Blood gDNA Kit, Large3840 prepsMAG-T-GDNA-L
Axygen AxyPrep MAG FFPE (DNA-RNA-miRNA) KitThe AxyPrep MAG FFPE nucleic acid purification kit utilizes a magnetic beads-based tech nologyto isolate nucleic acids (both DNA and RNA) from a maximum input of 10 mg of formalin-fixed,paraffin-embedded tissue. The protocol can be performed in both 96 well plate (manually andautomated) and in 1.5 mL tubes. Nucleic acid extraction begins with the addition of a reagentthat melts paraffin and de-crosslinks nucleic acids. Proteinase K is then added to complete tissuedigestion and inactivate nucleases. Next, binding buffer is added to facilitate immobilizationof the nucleic acids to the surface of paramagnetic beads. The contaminants are washed awayusing a simple washing step.M1234ApplicationswDNA sequencingwGene expressionwGenotyping5 µL DNA Solution was loaded intoeach well, Elution Volume 40 µLMAxygen 1 Kb DNA LadderLane 1,2CompetitorLane 3,4AxygenMagnetic Beads KitsKit SizeCat. No.AxyPrep MAG FFPE (DNA-RNA-miRNA), Medium96 prepsMAG-FFPE-MAxyPrep MAG FFPE (DNA-RNA-miRNA), Large384 prepsMAG-FFPE-L13
Axygen IMAG Handheld Magnetic Beads Separation DevicesThe use of magnetic separation devices (MSDs) are essential in any paramagnetic beadsbased purification process. Traditionally, MSDs are not optimized for manual use and mostrequire electrically powered liquid handling systems. IMAG MSDs are available in tube ormicroplate format and are designed to simplify the manual processing of magnetic beadseparations, including nucleic acid purification and clean-up, cell based assays, and antibody and protein purifications.ApplicationswNucleic acid purificationand clean-upwCell based assayswAntibody and protein urificationspwIMAG MSDs accommodate single tube or 96 well microplate formatswFast separation time- Strong magnets enable less than 30 second separation times- Holds tubes or microplate enabling fast wash stepswManual operation enables low or high throughputIMAG-96P: Separation of magnetic beads in microplate format10 seconds after separation process20 seconds after separation process30 seconds after separation processIMAG-12T: Separation of magnetic beads in tube format10 seconds after separation process1420 seconds after separation process30 seconds after separation processHand-held Magnetic Beads Separation DevicesPackagingCat. No.IMAG magnetic hand-held device for screw cap tubes1 unitIMAG-12TIMAG universal magnetic hand-held device for PCR and 96 well plates1 unitIMAG-96P
Compatibility ChartAxygen AxyPrep MAG KitUse It ToApplicationsAxyPrep MAG DNAConcentrateConcentrate purified DNAfrom diluted eluateSanger and Next Generation(NGS) SequencingAxyPrep MAG DyeCleanEfficiently remove primerdimers from PCR ampliconsSanger and NGS, Post PCR clean-upAxyPrep MAG FFPE(DNA-RNA-miRNA)Extract nucleic acids from lowamounts of sampleDiagnostics development,sequencing, qPCRAxyPrep MAGFragmentSelect-IClean and select desired DNAfragments from 150 bp to 1 kbNGS on Illumina and IonTorrent , library constructionAxyPrep MAG PCR Clean-UpClean-up PCR ampliconsSanger and NGS, genotyping,SNP, cloningAxyPrep MAG PCRNormalizerAvoid quantification bynormalizationNGSAxyPrep MAG Plant gDNAExtract gDNA from planttissuesSanger and NGS, genotyping,SNP, qPCRAxyPrep MAG PlasmidExtract plasmid DNA frombacteriaSanger and NGS, PCRamplification, restrictionenzyme digestionAxyPrep MAG Soil, Stool andWater DNAExtract DNA from soil, stool,and water samplesSanger and NGS, genotyping,SNP, qPCRAxyPrep MAG Tissue-BloodgDNAExtract gDNA from blood and mammalian tissuesSanger and NGS, genotyping,SNP, qPCRAxyPrep MAG Viral NucleicAcidExtract nucleic acid from RNA/DNA samplesSanger and NGS, genotyping,SNP, qPCR15
Beginning-to-endSolutions forGenomicsIn our continuous efforts to improve efficiencies and develop new tools and technologies for life scienceresearchers, we have scientists working in Corning R&D labs doing what you do every day, across the globe.From collection to analysis, our technical experts understand your challenges and your need for simplified,efficient, low- to high-throughput genomics processes.A combination of global manufacturing expertise, extensive use of in-house automation, an unsurpassedcommitment to product innovation and a thorough understanding of your processes enables Corningto offer a beginning-to-end portfolio of high-quality, reliable consumables and reagents for solutionsFor additional Axygen product or distributor information, please e-mail us at CLSCustServ@Corning.com, visit our website at www.corning.com/lifesciences/axygen or call 1.800.492.1110.Outside the United States, call 978.442.2200.Corning IncorporatedLife SciencesFor Axygen technical information, please e-mail us at AxgSupport@Corning.com or call1.800.429.9436. Outside the United States, call 510.494.8900.836 North St.Building 300, Suite 3401Tewksbury, MA 01876t 800.492.1110t 978.442.2200f 978.442.2476www.corning.com/lifesciencesFor a listing of trademarks, visit us at www.corning.com/lifesciences/trademarks.All other trademarks are the property of their respective owners. 2014, 2015 Corning Incorporated. All rights reserved. Printed in USA 4/15 CLS-A-BS-012 REV2Warranty/Disclaimer: Unless otherwise specified, all products are for research use only. Not intended for use indiagnostic or therapeutic procedures. Not for use in humans. Corning Life Sciences makes no claims regardingthe performance of these products for clinical or diagnostic applications.
eluted in 50 μL nuclease free water. 5 μL template was added into a 20 μL RT-PCR reaction mix. Each lane was loaded with 5 μL RT-PCR product. . AxyPrep MAG DNA Concentrate Kit is designed for both manual and fully automated purification of sequencing products. It can be used with most automated liquid handling workstations.
