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MODEL QUESTION IN MICROBIOLOGYDEPARTMENT OF MICROBIOLOGYCHAPTERS -CHROMATOGRAPHYA. MCQI. In gel filtration chromatography, separation of proteins are based on theira) size and net chargec) size and specific affinityb) shape and net charged) size and shapeII. The use of insulin hormone to purify its receptor is an example ofa) Ion exchange chromatographyc) Gel filtration chromatographyb) Affinity chromatographyd) Ligand mediatedchromatographyIII. Stationary phase in paper chromatography isa)solidb)liquidc)gasd)semi solidIV. In which of the following separation method where proteins are separatedon the basis of their net chargea) Affinity chromatography b) Ion exchange chromatography c) dialysis d) gelfiltration chromatographyV.) Using paper chromatography, the Rf value for xanthophyll was 0.3 and thevalue for chlorophyll a was 0.7. What is true for these two pigments?a) Xanthophyll is more soluble than chlorophyll a.b) Chlorophyll a is a larger molecule than xanthophyll.c) Chlorophyll a travelled farther up the paper thanxanthophyll.d) Chlorophyll a is more polar than xanthophyll.VI What is the maximum Rf value for any molecule inpaper chromatography? a)1.0b) 0.1c)10d)INFINITYVII. Which of the following gases is unsuitable for use as aGC carrier gas?a) Nitrogenb) Heliumc) OxygenVIII. In reverse phase chromatography, the stationaryphase is madea. non polarb.polarc) either non polar or polard)none

B. Answer the following1. What do you mean by Rf value? If Rf value of an analite is zero and one whatdoes it means?2. What is theoretical plates in column chromatography?3. What is the principle of paper chromatography?4. What is steric Hindrance? What should be the nature of matrix in affinitychromatography?5. What is retention time in GLC? How band broadening can be prevent in GLC.6. What is the full form of HPLC and its basic principle?7. What do you mean by counter current distribution?FOR SEMESTER THREE EXAMINATIONSUBJECT-MICROBIOLOGY (HONOURS)PAPER –SEC-1COURSE CODE-BMCBSEHT305A. Answer the following MCQ ( THE CORRECT ONE )2 X25 501. Anabena, a nitrogen fixer is present in the root pockets ofa) Salviniac) Azollab) Marseliad) Pistia2. Which of the following is Nitrogen fixing bacteria associated with roots ofsugar cane plantsa)Azospirillumd) Bacillus polymyxab)Azatobacterc) Clostridium3. IPM stands fora) Integrated pest manufactureb) ) Integrated plant management

c) ) Integrated pest managementmanagementd) integrated pesticide4. Which one is green maurea) Sesbeniab)Ricec) Oatd ) Maize5 . Which is the most common species of Azolla in India?a) Azolla pinnata.d) Azolla maxicanab) Azolla boliviensisc) Azolla prisca6. Which is the suitable medium for Azotobacter?a) Nitrogen free agar medium, e.g., Ashby’s medium.b)nitrogen free manitol brothCLED AGARc) blood agar mediad)7. The trigger for activation of toxin of Bacillus thuringiensis is:a) Acidic pH of stomach b). High temperaturec). Alkaline pH of gut d)Mechanical action in the insect8. The Ecto Mycorrhizias form an intercellular network in root cortex are calleda) arbusculesd) Haustoriab) vesciclesc) Hartig net9. The major advantage of plant with VAMa) increased nitrogen absorptionadsorptionc) increased k absorptionabsorptionb)increased Pd) increased Mn10. Which one of the following bacteria is used for killing of diptera likeAnopheles,Culex Ades mosquitosa) Bacillus anthraxc) Bacillus thuringiensis serotype israellensisb.) Bacillus subtilisd) Bacillus papillae

11. Mosquito fern isa) Azolla pinnata.b) Azolla boliviensisc) Azolla priscad) Azolla maxicana12. which one of this following EPV can not be mass produced throughfermentationa) Bacillus popilliaeb) Bacillus thuringiensisc) Bacillus moritaid) Bacillus polymyxa13.Mycorrhiza was first observed by which of the following scientista) Crickb) Fischerc)Frankd) Funk14. In stem and roots of Sesbenia rostrata, which one of the followingbacterium produces nodules?a) Azorhizobiumb)Bradyrhizobiumc) Burkholderiad)Sinorhizobium15.What should be added to increase efficiency of biofertilizera) Calciumb) Potassiumc) Phosphorusd) Zinc16. Farmers have reported 50 % higher yield of rice by using biofertilizera) Azolla pinnatad)Mycorrhizab) Legume-Rhizobium symbiosisc)Cyanobacteria17. What is true about Bt toxin?a) The inactive protoxin gets converted into active form in the insect gut.b) Bt protein exists as active toxin in the Bacillus.c)The activated toxin enters the ovaries of the pest to sterilize it and preventits multiplicationd) The concerned Bacillus has antitoxin

18. Leaves of plant used as biofertilizer belongs toa) Hibiscusb) Mango c) AnabenaD Azolla19. If wheat field is inoculated with Rhizobiuma) Soil will become nitrogen richnitrogenb) No effect on soilc)Soil will be depleted of nitrogenin calciumd) Soil will become rich20. Mycorrhiza is symbiotic association betweena) Fungus and Gymnosperm stemleavesb) Fungus with angiospermc)Fungus with legume fruitsand angiosperm roots.d)Fungus with Gymnosperm21. Viruses that are pathogenic to insects are found in the familiesa)Baculoviridaeb) Adenoviridaec)Bornaviridaed Gammaflexiviridae22. The genes responsible for producing Bt toxin are different gene products ofa) nod genesd)oncogenesb) nif genec) cry genes23. Commonly used seed pelleting agents area)calcium carbonate, rock phosphate, charcoal powderb) Dextranthe abovec) xanthan gumd) all of24.Which of the following forms symbiotism in soya bean crops?a)Azatobacter paspalid)Rhizibiumb) Bradyrhizobiumc) Nostoc

25. Name one fungal insecticidea)Beauveria bassianab) Metsukeminc ) Hirsutella thompsonid) both a and ce) both b & cFOR-U.G SEMESTER IV EXAMINATIONCOURSE TYPE-SECPAPER NAME-MICROBIOLOGICAL ANALYSIS OF AIR AND WATERCOURSE CODE-BMCBSEHT405ANSWER THE FOLLOWING MULTIPLE CHOICE QUESTIONS (TICK THECORRECT)2X25 501. How does one get rid of cyst of Giardia lamblia and Cryptosporidium ,whichare resistant to chlorination of watera) by filtrationpasteurizationb) by deodorization c) by incineration d) by2. All over microbial processes that occur in soil ,water or food refer as whichone of the followinga) Environmental microbiologyb) Microbial ecologyc) Microbial physiologyd) Microbial taxonomy3. Disinfection of water during water purification is usually done withapplication of which of the following chemicala)chlorine b)ozone c)both a and bd)none4.Most of the indicator organisms for detection of disease occurrence level indrinking water are belongs to which of the following organisms groupa) Actinobacteria b)Bacillid)Firmicutesc)coliform

5. Which of the following is the amount of dissolve oxygen needed formicrobial oxidation of biodegradable organic mattera)BODb)CODc)TOCd) all of the above6 .In air, which of the following device is used for the enumeration of bacteriaa) Anderson air samplerb) Capillary impinge c) Lemon sampler d) all7. Fine organic or inorganic particles suspended in air is calleda)Particulate pollutants b)Aerosols c)Gaseous pollutants d) None8. Examples of air borne disease area)flue, tuberculosis and valley feverc) both a and bb) AIDS ,polio’ and cholera feverd) none9. Air borne diseases can be caused by the followinga) Viruses, bacteria, fungic) Usually virusesb) Only bacteriad) only fungi10. Biological treatment of sweage by microorganism (mainly decomposer)would most likely occur at which stage of waste water treatmenta) Primary b) secondary c)Tertiary d) all11. The B.O.D would be most directly affected by the presence of which of thefollowing pollutantsa)Heavy metal b)organic waste c)salt d) Waste minerals from mining12. Coliform test are related to the test ofa) Drinking waterproductsb) sterility check of foods c) quality of milk and milkd)all of the above13. In which of the following treatment process 90 to 95 percent of the BODand many bacterial pathogens are removed

a)Primary b)secondary c)tertiaryd) Quatenary14.Trickl;ing filter are used for the removal of organic waste from waste waterby secondary treatment is aa)Aerobic process b) Microaerophilic process c)Anaerobic process d) all15.Which of the following nutrients converts to the water system fromoligotrophic to eutrophic conditiona)Nitrogend) noneb)Phosphorusc)both a and b16. The no of bacterial colonies by Agar plate count test should not exceed --------per ml for potable watera)1b)10c)100d)100017. .medium is used in the presumptive testa) Lauryl sulphate-tryptone brothbrothb) Macconkeysc) Brilliant green lactose bile brothd) TSI BROTH18. High efficiency particulate air filters(HEPA) are used in laminar hoods toremove the particlesa) 0.1 µmb) 0.3 µmc)0.5 µmd)0.25 µm19. Which of the following test is used as presumptive test for enumeration ofcoliform in water samples?a) Most probable no (MPN)b) Heterocoliform count (HCC)C) aerobic colony count (ACC)d) none of the above20 . are used frequently as indices of fecal pollution in water and food.a) Only E.colib) E.coli and streptococcus fecalis

c) E.coli and Cl. perfringensstreptococcus fecalisd) Cl. perfringens&21. In MPN test what is the amount of water you should add in each doublestrength lactose broth(2X) tube?a) 2.5 mld)100 mlb) 5 mlc)10 ml22. Measles is an airborne disease that circulate mainly througha) Sneezingb) Coughingc) hand shaked ) both a and b23. The following are waterborne diseases excepta)Cholerab)Typhoidc) Hepatitis Ad)Measles24. ----------------------represents the bacterial density that is most likely to bepresent in watera) BODb) CODc) MPNd) COLIFORM INDEX25. which of the following statement is/are correct regarding coliform bacteria?1. it is gram negativemicroorganismsa)1,2,32. It is rod shaped bacteria 3. It is a harmless aerobicb)2,3MODEL QUESTION MCQc)1,3d)1,2

Who was the first to record that Azospirillum lipoferum (old name, Spirillumlipoferum) fixes up atmospheric nitrogen?Ans: Beijerinck in 1925.Q. which of the following is used as green manureA) BacteriaB) MycoplasmaC) NostocD) None of theseA protoxin is:a. A primitive toxinb. A denaturedtoxinc. Toxin produced by protozoad. Inactive toxinQ.which acquatic plant is used to increase the yield of paddy fielda)marsileab)Azollac)salviniad)all of this

Who was the first to record that Azospirillum lipoferum (old name, Spirillumlipoferum) fixes up atmospheric nitrogen?Ans: Beijerinck in 1925.which of the following is used as green manureA) BacteriaB) MycoplasmaC) NostocD) None of theseA protoxin is:a. A primitive toxinb. A denaturedtoxinc. Toxin produced by protozoad. Inactive toxinwhich acquatic plant is used to increase the yield of paddy fielda)marsilea

b)Azollac)salviniad)all of this

SEMESTER I MODEL QUESTIONS1. What is PPLO?2.what is chainia3.what is glycocalyx4.What is metachoromatic granules ?5. What is the principle of acid fast staining? Name two acid fast bacterialspecies6. What is “hopanoid7. Why is the antibiotic penicillin effective against Gram positive bacteria butnot harmful to human R.B.C?8.What do you mean by eubacteria?9.What is L forms of bacteria?10. name one carbon reserve material present in bacteria.11.mention two important contribution of Pasture(also read kotchspostulates,golden period of microbiology )1. Name the polymeric substance found in bacterial cell as inclusion and can bestained by Sudan black.2. Name one gram negative beneficial bacterial species &one gram negativehuman disease causing bacterial species.3. What is the role of acid alcohol in ACID FAST STANING procedure?4. Who discovered kingdom Protista?5. Name the scientist called as father of Medical microbiology.6. What is the full form of EMB agar media? And what is the role of this agarmedia in water testing procedure.7. Name the bacterial species transforms extracellular iron to the mixedvalence iron oxide mineral magnetite (Fe3O4) and construct to the intracellularmagnetic compasses.8 .Which periods is regarded as the golden period of Microbiology?