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Compact Space Saving Design Uses minimal bench space with built-in fractioncollector and integrated touchscreen.Powerful Easy-to-UsePeakTrak Control Intuitive PeakTrak software minimizes the learning curve,enabling the user to efficiently complete their purification,saving time and solvent consumption. Log in through a network connection and control theseparation from your desktop.Achieve High PerformanceLiquid Chromatography (HPLC)simply, without compromise.Standard System Features Flow rates from 1 to 150 mL/minallows analytical scouting and Prepmethods on one system Operating pressure up to 6,000 psi Choice of UV or UV-Vis plus ELSDand MS options One touch Focused GradientGenerator minimizes purificationtime while maximizing samplerecovery Complete control of the systemon one screen Lifetime of free software upgradesThe Teledyne ISCO ACCQPrep HP150 is designed tobe intuitive and easy-to-use with all the performanceand accuracy of an HPLC system. The system focuses onpurification with the highest sample recovery.With ease of purification in mind, the user-friendlyACCQPrep HP150 eliminates unnecessary and complexmethod parameters found in many of the HPLC systems intoday’s market. Our PeakTrak software, designed with theuser in mind, is the basis for controlling the ACCQPrepHP150. The integrated software, eliminates the need for astand-alone PC. This results in a more compact unit with atouch screen interface. Software evolves with user needsand updates are always free.Enjoy the confidence associated with Teledyne ISCO’sactive solvent and waste level monitoring, which reducesthe risk of solvent spills. In addition, never miss or overfilltest tubes again with RFID collection racks.The ACCQPrep HP150 creates high accuracy gradientsand low dwell volumes using dual piston, high pressurepumps. Operate across the full range of flow rates fromanalytical (1 mL/min) to 50 mm and larger preparativecolumns (150 mL/min), without the need to switch out pumpheads. Save method development time by running ascouting method on an analytical column, and scale upto prep on one system, with a single touch.One Screen Operation Start a separation in seconds. Choose a column, pressplay, load your sample, and walk away. On-the-fly chromatographic changes are easy to makeon the touchscreen, even during the run.Column Selector Valve(CSV-4 Optional) Users can run samples on up tofour different columns containingdifferent media or differentdimensions based on samplesize needs.Active Solvent andWaste Level Sensing Never run a column dry oroverfill a waste container.Rack Sensing System reads the RFID rack and setsthe fill volume avoiding tube overfillsor missed tubes. Supports a variety of collection racksizes so that purified compounds canbe collected in volumes appropriatefor the column size being used.Photo Diode Array Detection UV and UV-Vis detectors use PDA technology Display spectra in real time or post run Collect based on purity indicatorsteledyneisco.com2
Focused Gradient GeneratorIntegrated ELSDImproved Resolution is One Touch Away!Save time and solvent while increasing column loading.Designed to allow quick method optimization to get to your purified product by every chemist. Run a single scouting run, thenuse our exclusive Focus Gradient feature, where you are a touch of the peak away from an optimized gradient that maximizesefficiency and resolution around your target compound. This method is automatically scaled up to the column size (of matchingmedia) of choice. Seamless integration with our PurIon MS confirms you have selected the right peak to optimize.Collect weak UV absorbingcompounds with certainty. Fully-integrated into the ACCQPrep with noadditional bench-space needed. Offers scalability and sensitivity for a wide rangeof sample loading with easy-to-change methodparameters. Peak alignment automatically adjusted for varyingsolvent mixtures and different flow rates. Alignment from 2 to 150 mL/min with no userhardware changes.0.50 3.000.45 2.752.500.402.250.35 2.000.30 1.750.25 1.500.20 1.251.000.150.750.100.500.05 0.250.00 9.010.011.0 12.013.014.0 15.00.50 3.000.45 2.752.500.402.250.35 2.000.30 1.750.25 1.500.20 1.251.000.150.750.100.500.05 0.250.00 0.000.020 x150 @20 .09.010.011.0 12.013.014.0 15.01.00 3.002.752.500.75 2.252.001.75.050 1.501.251.000.25 0.750.500.250.0 0.000.0Scouting Run4.6 x150 @2 mL/min1001009030 x250 @42.5 mL/min807060504030201005.010.515.020.025.0No Chromophore—No Problem with ourELSD and MS modules!Mass-DirectedPreparative HPLCFocused RunThe PurIon Mass Spectrometer is fully integrated with Teledyne ISCO’s PeakTrak software. The ability tomanually inject samples prior to purification andcollecting based on mass(es), makes this Mass-directedACCQPrep system ideal for many applications.Focused Gradient Generatorpaired with the PurIon MassSpectrometerEasy to Use Method OptimizationRight the First Time! Using the Method Development screen, easilyadjust loading and ionization settings beforecommitting your valuable sample for purification.No more guessing which peak is your compound!Coupling a scouting run with MS data meansyou can now have confidence that the separationis optimized for your target compound. Gettingthings done right the first time, saves money,solvent and most importantly time. Use IonFinder to easily pick out more abundant MSadducts of your unique compounds. Use Terminate on Target to shorten your separationafter your desired mass is detected to save solventand time.Use of Focused Gradient Generator and PurIon MS to scale up purification of peptidefrom 4.6 x 150 mm column to 20 x 150 mm column.teledyneisco.comVerify before you purify! Collect on up to 6 masses or 5 masses and amass range4
Maximize the throughput and flexibility of your ACCQPrep system byoptions for longer unattended operation.addingautomation432TUBE CAPACITYWITH AS 2x2*Unlimited rack swappingwith AutoSamplersThe two rack AS 2x2 and four rack AS 4x2sampler options allow different chromatographicconditions to be applied to a variety of differentsamples and doubles or triples the fractioncollection capabilities of the base system.648TUBE CAPACITYWITH AS 4x2*Accessories for everyday simplicityWaste CollectionSwitching ValveThird SolventModifier PumpInterfaces with AS 2x2 and AS 4x2.Directs waste from up to 12 samplesinto separate waste reservoirs. Idealfor open access to separate individualsample wastes. Requires presence ofeither the AS 2x2 or AS 4x2.Controlled via PeakTrak and providesdelivery of a third solvent at a fixedpercentage during the purification.Solvent Select ValveColumn Select ValveEasily change your chromatographicselectivity by changing to a differentsolvent system. The 3 x 2 solvent modifierallows any combination of 3 differentA solvents with 3 different B solvents.Change between up to 4 differentinstalled columns with the CSV-4 module.Fully integrated automation with PeakTrakmakes changing stationary phases orsizes a breeze.RediSep PrepHPLC ColumnsMaximize Your PreparativeHPLC PerformanceLarge VolumeSample Load PumpPerfect for peptides and naturalapplications dilute samples. Injectvolumes of samples greater than10 mLdirectly onto the column bypassingthe injection loop. Pump control isintegrated with PeakTrak.When you need the highest puritycompound, your first choice shouldbe to equip your ACCQPrep HP150with RediSep Prep columns.Teledyne ISCO has built a qualityreputation on the high performanceof our flash purification columns.RediSep Prep columns are specificallydesigned for high performance liquidchromatography (Prep HPLC).Features: Prep column diameters ranging 10to 50 mm with lengths of 150 and250 mm, all in 5 µm media. Available matching UPLC (2 x 50mm, 2.8 µm) and analytical HPLC(4.6 x 150 mm, 5 µm) columns formethod development. Available stationary phases includebare silica, C18, C18Aq and C8chemistries. All matching availableflash stationary phase chemistries. Protect your column investment withour 20 x 30 mm guard columns withmatching stationary phase.*13mm tubesteledyneisco.com6
Purification, Performance, and Reproducibilitywithout CompromiseStart with the binary gradient base system or add the optional solvent selection valve to allow selection from sixsolvents. The minimal gradient delay design allows purification method development on the ACCQPrep HP150using a 4.6 mm analytical column. The method can be automatically scaled to a larger preparative column.Performance WithoutCompromiseGradientReproducibilityNo Need forTemperature ControlGraph 1 – Mixture of methyl and propyl parabenrun on a 10 x150 mm column at 19 mL/min with apressure of 5,000 psiGraph 2 – Gradient DelayThis gradient reproducibility results in repeatablechromatographic runsGraph 3 – Five Injection TestGraph 1 illustrates no compromise inperformance even when the system isoperating close to the maximum systempressure of 6000 psi and running flowrates that are higher than ideal.Graph 2 compares the programmedgradient against the profile obtained fromthree consecutive runs at 5 mL/min. Thegradient closely follows the programmedprofile, indicating minimal delay volumes,and the reproducibility is unmatched.Graph 3 shows five injections overlaidusing a test mixture of ethyl, propyl, andbutyl paraben. There is minimal retentiontime shifts over a three hour period without the use of any temperature control.Going Green and Saving Time in the LaboratoryAn important principle towards greener chemistry and processes is to “Maximize Efficiency: Meet Need, Minimize Excess.” One of theways the new ACCQPrep HP150 helps users implement this principle is with the new Focused Gradient Generator. The use of optimizedgradient methods maximizes efficiency (allowing purification with larger sample loadings) minimizing both the waste output and theamount of solvent used, all while saving user time. The use of focused gradients eliminates the need for over 80% of the default gradientprofile where the compound is not moving down the column or already eluted off.Reducing waste in our Chromatography line is just another way we are using our innovative products to increase productivity whileimproving the quality of life on our planet.Teledyne ISCOP.O. Box 82531, Lincoln, Nebraska, 68501 USAToll-free: (800) 228-4373 Phone: (402) 464-0231 Fax: (402) 465-3091teledyneisco.comTeledyne ISCO is continually improving its products and reserves the right to change productspecifications, replacement parts, schematics, and instructions without notice.L -5222Rev 3.001/ 21
Achieve High Performance Liquid Chromatography (HPLC) simply, without compromise. Standard System Features Flow rates from 1 to 150 mL/min allows analytical scouting and Prep methods on one system Operating pressure up to 6,000 psi Choice of UV or UV-
