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SciLog LabTec intelligent bioprocessing system dispensing systemThe Scilog LabTec is an automated laboratory scaledispensing system by volume, weight or weight ratio.The LabTec automates, optimizes and documents repetitive liquiddispensing with or without in-line filter sterilization. The LabTec models are ideally suited for dispensing sterile solutions in mediakitchens and microbiology laboratories. Also widely used in smallproduction runs to fill your final product into vials, bottles, bags,and containers.The automatic documentation and alarm/pump stop settingsallow the user to focus on other tasks while the system is running.Remote control and programmable end points ensure the systemstarts and stops operating when a given dispense is complete oran interlock condition occurs. When sold with SciDoc software or aprinter, documentation capabilities include 7 real-time parameters.Features and Benefits Accuracy /- 0.3% byweight Rapid, high previsiondispensing by volume,weight or weight ratio Pressure alarm alertswhen in-line filter needsto be replaced Self priming Reversible flow PC or printerdocumentation ofdispensing Remote activation Accuracy /- 0.5% byvolumeNote: LabTec is a registered trademark of Parker Hannifin Ltd.Performance CharacteristicsTable 1 - Automated Weighingand Dilution of Food SamplesTable 2 - High SpeedVolumetric 089.9310.090.090.1 0.1410.090.089.8-0.2510.090.090.1 0.1610.090.090.0 0.1Table 3 - GravimetricSolution )SlowFactor(ml)TypicalPrecision(%)DispensingTime 50.15%2.9Errors(g)710.090.089.9-0.1810.090.090.2 0.2910.090.090.1 24100%4.750.35%6.3100.0#35100%5.00 0.5%4.5200.0#35100%5.00 0.5%6.0300.0#35100%5.00 me .25%10WeightEntered(g)
SciLog LabTec Applications2. High Speed, High Precision VolumetricBatch DispensingWeighing, Dilution and Sterilization4. BiopharmaceuticalGMP FillingApplication:Fast,Accurate Dispensingand SterilizationPrecision Batch DispensingHigh Accuracy Dispensing with In-Line Filter SterilizationLabTec LabTec In this configuration, the LabTec is connectedto an electronic scale for high accuracy fillingapplications. A sterilizing filter and a disposablepressure transducer provide a safe and effectivein-line filter sterilization capability. The LabTec continuously monitors the filter backpressure andalarms when a user-defined pressure level hasbeen exceeded, which indicates a filter plug-upcondition. See Table 3. The high dispensing accuracyis achieved by reducing the pump rate as final targetweight is being approached. The slow-down avoidsovershooting the target weight. After slow-down, theLabTec pump stops briefly when 99% of the finaltarget weight has been dispensed. The electronicscale is allowed to come to a steady-state readoutand the LabTec slowly starts up again to dispensethe remaining solution.The LabTec Smart Dispensing System is capableof high speed, high precision batch dispensing.The LabTec comes with a 1082 Tandem peristalticpump head. Up to 10 different dispensing volumescan be stored and easily retrieved for quick batchvolume dispensing. An optional sterilizing filter anddisposablepressuresensorforDispensingin-line bufferLabTecPerformanceData: HighSpeedprovideVolumetricsterilization and reliable detection of filter tor: resultsPrecision:includingTime/Aliquotdispensing times are summarized in Table 2. The5.00 ml #15100%2.50 ml1.7%2.1 Secis#15easy to100%calibrate.A storedLabTec10.00 ml2.50 ml0.5%calibration2.0 Sec25.00 mlis provided#15100%ml ination.a storedyou select25.00 ml#24 From100%4.75 menu,ml0.15%2.9theSec 50.00 ml tubing#24 you have100% installed4.75 ml in the0.50%LabTec3.9 .SecForpump100.00 ml#24100%4.75 ml0.35%6.3 Sechigh accuracy dispensing applications, you may want 100.00ml the #355.00 ml 0.5 % feature.4.5 Sec’s single-pointre-calto useLabTec100%200.00 ml450.00 ml#35#35100%100%5.00 ml5.00 ml 0.5 % 0.5 %6.0 Sec12.0 SecNote: This performance data was generated with a 600 RPM pump motor and Tandem 1082 peristalticpump head. The pump tubing was made of SiliconeSpecificationsDescriptionDimension / WeightWidth: 5.75 (146 mm) x Height: 8.5 (2126 mm) x Depth: 11 (279 mm): 14 lbs (6.4 Kg)Enclosure & Rating16 Ga, aluminium baked epoxy blue 4-40dC, 0-100% Humidity, IP20Accommodates one (1) disposable pressure sensor. The calibrated pressure range is 0 - 60 psi. Any point withinthis range can be recalibrated using an external pressure reference source.Pressure SensorsPower115 / 220-240 VAC, 60 / 50 Hz, 75 Watts, double fused: T1AL 250V (CE: IR35A 250VAC)Motor / Encoder8, 160, 600, 3400 RPM, 30 VDC, 3.8A, 100 pprMale DB9 Scale Connections (RS-232), Female DB9 Printer or PC Connection (RS-232), External IO DB37connector, 1 TTL input, 4 TTL output, 3 4-20mAI/O PortsOperational ModeDispense by volume, weight or weight ratio. Store up to 10 programs per mode. Pump re-calibration.Options and AccessoriesPump Heads:Tandem Peristaltic 1081 Flow Rate (ml/min): 0.03 - 1515 1082 Flow Rate (ml/min): 0.5 - 2258(Pressure: 25 psi continuous - 45 psi max) Masterflex Peristaltic:0.03- 2900(8, 160. 600 RPM)(Pressure: 25 psi continuous - 45 psi max) FMI RH Piston:0.002-320 (600, 3400 RPM)(Pressure: 100 psi max) Micropump MAG:0.54-3488 (3400 RPM)(Pressure: 40 - 70 psi max[model dependant] )Ordering Information20- LABT-1Code Electricity Input01120 VAC220 VACPump HeadCode Motor01368 RPM160 RPM3400 RPM600 RPMCode ScilogCode MasterflexCode Micro Pump8182212262313233341081 Pump1082 PumpThin WallThick WallHi Performance L/SMAG 120MAG 201MAG 1840MAG 040Code FMI RH353637MAG 200MAG 201MAG 1874142OCKC1CKCExample: 200-LABT-1181 - SciLog LabTec - 120 VAC,160 RPM motor and 1081 headParker domnick hunter has a continuous policy of product development and although the Company reserves the right to change specifications, it attempts to keep customers informed of any alterations. This publication is for general informationonly and customers are requested to contact our Process Filtration Sales Department for detailed information and advice on a products suitability for specific applications. All products are sold subject to the company’s Standard conditions of sale.DS P 17 02/13 Rev. 1B‘ 44 (0)191 4105121 dhprocess@parker.com - North America: toll free: 800 955 1993 dhpsales.na@parker.com www.parker.com/processfiltration www.scilog.comThe LabTec significantly increases the productivityof any microbiology laboratory. The dispensingsystem automates weighing and diluting of foodLabTec PerformanceData: AutomatedWeighing andsamples,in preparationfor bacteriologicalanalysis.Dilution of Food SamplesOnlyanapproximatesamplesizeis needed. TheTrial idlyLabTec#WeightDiluentWeight andDiluentWeight dispenses(grams)the1 media10.0grrequiredto90.0grachieve a user-selected.90.0gr0.0210.0 See Table90.0 1. Costly and89.9 time- - 0.1diluentfactor.310.090.090.1 0.1410.090.0of media is89.8- 0.2consumingautoclavingavoided with510.090.090.1 0.1in-linefilter10.0sterilizationusing a filter690.0 of diluents90.0 0.1710.090.089.90.1capsule.A disposablepressure sensormonitors810.090.090.2 0.29 backpressure10.090.0provides an90.1 0.1filterandalarm signal1010.090.089.8- 0.2when a failing sterilizing filter (highbackpressure)A VE:89.99needs to be replaced. An optionalprinteror PCSD:0.14RSD:0.15%hook-up automatically documentsallsample andLabTec Dispensing Parameters: Weight Factor 9.00, Slow Factor 25gr, Pump Rate 50% ne pumptubing was usedfor all dispensedaliquots. Allsolutions were dispensedthrough a PallSuporCap 50 sterilizing filter. Cycle Time: 25 sec.; Diluent Dispensing Time:14 sec.the speed, precision and accuracy of the entiresample preparation process by automating theweighing, dilution and documentation process.LabTec
SciLog MabTec intelligent bioprocessing system automated high density cell culture systemThe SciLog MabTec is an automated high density cellculture system.The MabTec high density cell culture system is a fully automatedgravimetric bioreactor maintenance system. Its capabilities includeautomated feed, harvest or recirculation in fully disposable orhybrid bioreactor flow paths. The system also includes the abilityto conduct unattended bioreactor inoculations, bolus feed and/orpump flow reversals (to prevent filter fouling) all on one unit withrun times from hours to months.The MabTec is available with a choice of pump heads and can feedbioreactor sizes from 50mL to 2000L. The MabTec is engineered tofit seamlessly with any bioreactor and enhance its performance.A laboratory scale, available separately, must be connected to usethe MabTec ’s gravimetric features.Features and Benefits Works with any bioreactor Safe, walk-away systemoperation Eliminates bioractoraddition errors Maintains a steady statebioreactor weight / volumewithin /- 0.5% Reduces aggregation ofcells in the perfusion filter Real-time data collectionwith optimization tools Intuitive applicationinterface Small, lightweight andmobileNote: MabTec is a registered trademark of Parker Hannifin Corporation.MabTec motor size, pump head and tubing recommendationsvs reactor size and reactor exchanges per dayReactor Volume Size#Exchange per day0.05 0.251.5210.03 0.17 0.35 0.69 1.0420.07 0.35 0.691230.10 0.52123440.14 0.69134650.17 3#24#16#152#148 RPM108110823#154410825102050100250500 1000 20003714356917434767142869139347694 13898810102142104208521 1042 2083111114142856139278694 1389141417173569174347868 17362828213569139347694 17364252104208521 10425669139278694 1389#24#15#14#16160 RPM10815#248#15#24160 RPM1081*Flow rates in grams per minute#35600 RPM1082694 13898 RPM with 1081 and #13 tubing8 RPM with 1082 and #15 tubing8 RPM with 1082 and #24 tubing160 RPM with 1081 and #14 tubing160 RPM with 1081 and #16 tubing160 RPM with 1082 and #15 tubing160 RPM with 1082 and #24 tubing600 RPM with 1082 and #15 tubing600 RPM with 1082 and #24 tubing600 RPM with 1082 and #35 tubing
Fig. 1 Pre-Programmed ModesThe MabTec has a pre-programmed modefor inoculating, transfecting and inductionwhen used with a laboratory scale.This pre-programmed mode allows the userto deliver precise additions without beingphysically present, which is ideal for processesthat are scheduled to run at inconvenienthours or on a repeated schedule.Fig.2 - Fed BatchThe MabTec can be used to automate thefeeding of cell cultures. Automation of thisprocess greatly reduces the possibility ofhuman error while reducing operator time.The MabTec can be programmed to deliver acontinuous feed for a specified number of daysup to 100. Two modes exist for feeding; onethat monitors levels in the reactor and makesadditions while the other monitors the reactorand amount of feed media.Fig. 3 - Ultra High Density Cell CultureThe MabTec system is uniquely designedto provide a complete perfusion strategy.The user is able to maintain fresh media inthe reactor, remove the desired componentor prevent the build-up of waste products,all without a scale under the bioreactor.By keeping bioreactor weight maintained towithin 2% and providing a constant streamof nutrient rich and pH buffered media, thebioreactor environment becomes ideal forultra high density cell cultures.SpecificationsDescriptionDimension / WeightWidth: 5.75 (146 mm) x height: 8.5 (2126 mm) x depth: 11 (279 mm): 14 lbs (6.4 Kg)Enclosure & Rating16 Ga, aluminium baked epoxy blue 4-40dC, 0-100% humidity, IP20Accommodates three (3) disposable pressure sensors [included]. The calibrated pressure range is 0 - 60 psi. Anypoint within this range can be recalibrated using an external pressure reference source.Pressure SensorsPower115 / 220-240 VAC, 60 / 50 Hz, 75 Watts, double fused: T1AL 250V (CE: IR35A 250VAC)Motor / Encoder8, 160, 600 RPM, 30 VDC, 3.8A, 120 ppr 8 and 160 RPM, 600 RPM 100 pprMale DB9 scale connections, female DB9 printer or PC vonnection, external IO DB37 connector 4 TTL input, 34-20mA analog input: constant rate / constant pressure filtration with size user-definable alarmsI/O PortsOperational ModeExact Feed: 2 scales, perfusion, re-circulation, feed: 2 scale, feed: 1 scale, seed induction, manualOptions and AccessoriesAccessories:Pump Heads: Pressure: 1081 Flow Rate (ml/min): 1082 Flow Rate (ml/min):25 psi continuous45 psi max.0.03 - 15150.5 - 2258 SciPres Pressure sensors Fittings and tubing kit Manifolds Printer Master slave cables Laboratory balance WeighStation Ordering Information- MTEC - 120Code Electricity Input01120 VAC220 VACCode Motor0168 RPM160 RPM600 RPMCode Pump Head81821081 Pump1082 PumpExample: 200-MTEC- 1181- SciLog MabTec - 120 VAC with scale, 160 RPM motor and 1081 headParker domnick hunter has a continuous policy of product development and although the Company reserves the right to change specifications, it attempts to keep customers informed of any alterations. This publication is for general informationonly and customers are requested to contact our Process Filtration Sales Department for detailed information and advice on a products suitability for specific applications. All products are sold subject to the company’s Standard conditions of sale.Europe: 44 (0)191 4105121 dhprocess@parker.com - North America: 1 608 824 0500 dhpsales.na@parker.com www.parker.com/dhsingleuseSciLog MabTec DS P 21 07/13 Rev. 1B
SciLog PureTec intelligent bioprocessing system tangential flow filtration systemThe SciLog PureTec is a laboratory-scale tangentialflow filtration (TFF) and parameter development system.The PureTec system is delivered as a complete developmentsystem including three pressure sensors, an 8.1kg balance,a 500mL vessel with magnetic stirrer and startup kit includingtubing and fittings. Methods can be performed using constantor step cross flow, inlet or transmembrane pressure (TMP).The automatic documentation and alarm / pump stop settingsallow the user to focus on other tasks while the system is running.Programmable end points ensure the system stops operatingwhen a user defined concentration or diafiltration is reached. Whensold with SciDoc software or a printer, documentation capabilitiesinclude 17 real-time filtration parameters such as TMP, permeateflow rate and quantity, Normalized Water Permeability (NWP),concentration factor, and other parameters over time.Features and Benefits Complete developmentsystem Flow and automatedpressure control Real-time data collectionwith optimization tools Safe, walk away systemoperation Scalable parameters,easy tech transfer Intuitive applicationinterfaceNote: SciLog & PureTec are registered trademarks of Parker Hannifin Corporation.Performance Characteristics180250WFI2003 wt% Protein1505 wt%1008 wt%500Retentate Flow Rate: 80 ml/minRetentate Flow Rate: 50 ml/min160Permeate Flux (J), LM2HPermeate Flux (J), LM2H300TMP max 17 psi51015202530J Flux K Ln [Cp/Cm]120Cp Protein Concentration in SolutionCm Protein Concentration at membrane10080Slope 1 k’ Mass Transfer Co-efficient60Slope 2 k 4020*TMP [(P1 P2) / 2] - P3014035Transmembrane Pressure (TMP)*, psiFig. 1 - Transmembrane Pressurevs. Permeate Flux0Cm1.010.0In TFF, concentration procedures, a diluteprotein solution ( 1.0 g/L) is re-circulated,through a TFF filter whose selected porosityallows only solvent (water) and dissolved salt topass through the filter pores and is collectedas permeate. The removal of water leaves anincreasingly concentrated protein solution behindas retentate. The gradual removal of watercauses a gradual increase in solution viscosityduring the TFF concentration step. Permeate flux,transmembrane pressure (TMP) and cross flowrate are significantly affected by solution viscositychanges.100.0Ln (Protein Conc. Wt %)Fig. 2 - Ln [Protein Conc, (CP)]vs. Permeate Flux (J)In order to maintain an optimal permeate flux,both cross flow rate and measured TMP requirefrequent, manual adjustment during the TFFconcentration procedure. The PureTec hasthe control methods to perform the operationautomatically via constant or stepped cross flowrate, inlet pressure or transmembrane pressure.The system will automatically stop when the userdefined concentration factor has been reached.
SciLog PureTec ApplicationsComponents not to scaleConcentrationDiafiltration via SuctionComponents not to scaleDiafiltration via ChemTec Components not to scaleCHEMTEC ChemTec PureTecPURETECIn the constant pump rate or pressure modes,you can implement your selected pump rateor pressures and use some or all of five alarmconditions. The pump rate or pressures can beincreased or decreased “on-the-fly” withoutstopping the pump action.In the constant rate mode, increasing the feed ratein a stepwise fashion and simultaneously monitoringthe permeate collection rate allows you to readilydetermine the optimal feed rate with the highestpermeate yield (See figure 2).In the constant pressure mode, increasing thetrans-membrane pressure (TMP) in a step-wisefashion and simultaneously monitoring thepermeate collection rate allows you to determinethe optimal TMP with the highest permeate yield(See figure 1).In diafiltration procedures, a protein solution is “washed”, i.e. de-salted, using an exchange buffer to removeundesirable electrolyte (dissolved salt). The concentrated protein solution is re-circulated, through a TFFfilter whose selected porosity allows only the undesirable electrolyte to pass through the filter pores, whichis collected as permeate. The permeate volume (undesirable electrolyte) that has been removed from theconcentrated protein solution (retentate) is replaced with an (desirable) exchange buffer. In constant volumediafiltration or “washing” procedure, the collected electrolyte is automatically replaced by an equal volume ofexchange buffer. Approximately ten (10x) volume exchanges are typically required for substantial removal ofundesirable electrolyte from the concentrated protein solution.Diafiltration via suction:For small volumes a SciLog pressure vessel storedwith buffer can be connected to the secondary inletport on the retentate vessel. While keeping a closedsystem the retentate vessel will automatically drawout buffer to replace the salt and solvent that hasbeen removed by the filter. The process will continueuntil the PureTec has reached its programmableend points.Diafiltration via ChemTec :For larger volumes the SciLog ChemTec systemand an appropriately sized balance will monitor andmaintain the fluid in the PureTec ’s retentate vessel.The ChemTec will maintain the level until a userdefined volume exchange has been reached.SpecificationsDescriptionDimension / WeightWidth: 5.75 (146 mm) x height: 8.5 (2126 mm) x depth: 11 (279 mm): 14 lbs (6.4 kg)Enclosure & Rating16 Ga, aluminium baked epoxy blue 4-40dC, 0-100% humidity, IP20Pressure SensorsPowerAccommodates three disposable pressure sensors. The calibrated pressure range is 0 - 60 psi. Any point withinthis range can be recalibrated using an external pressure reference source.115 / 220-240 VAC, 60 / 50 Hz, 75 Watts, double fused: T1AL 250V (CE: IR35A 250VAC)Motor / Encoder8, 160, 600 30 VDC, 3.8A, 120 ppr 8 & 160 RPM, 100 ppr 600 RPMMale DB9 scale connections (RS-232), female DB9 printer or PC connection (RS-232), exter
culture system. The MabTec high density cell culture system is a fully automated gravimetric bioreactor maintenance system. Its capabilities include automated feed, harvest or recirculation in fully disposable or hybrid bioreactor flow paths. The system also includes the ability to cond
