WIXLIB

High-speed, cutting-edgespectral sorting and analysisThe Bigfoot Spectral Cell Sorter provides power,safety, performance, and flexibility for your lab

Discover the cutting-edgeBigfoot Spectral Cell SorterThe Invitrogen Bigfoot Spectral Cell Sorter with Sasquatch Software (SQS) provides the power, safety,performance, and flexibility for your lab today and into the future.With a custom-designed, integrated Class II biocontainment cabinet, the Bigfoot Spectral Cell Sorterprovides safety and protection without compromising high-parameter sorter performance. Multitubeinput paired with 18-way virtual sorting and integrated temperature control gives flexibility for allyour applications.With a stand-alone footprint, no ancillary requirements, and high-speed electronics enabling 100,000eps acquisition and 70,000 eps sorting and hot-swap fluidics, the Bigfoot Spectral Cell Sorter providesunmatched performance in any lab space.The Bigfoot Spectral Cell Sorter can be configured with up to nine lasers and 60 detectors, providing theversatility for both standard fluorescence detection and spectral unmixing. Multiple scatter options allowsimultaneous standard and small particle detection, multilaser scatter detection, and/or polarization.SQS provides quick start-up, automated calibration, and accurate quality control combined with anexperiment designer, intuitive interface, and efficient shutdown, allowing the system to be easy-to-usewhile reducing downtime. Remote access capability, system health information, and email notificationssave time and streamline your workflow.23

SpectralHigh throughputSorting and analysisHigh speedThe first-of-its-kind Bigfoot Spectral Cell Sorter allows both spectral sorting andspectral analysis on cell populations.Configurable, quick, and calibrated, the high throughput ofthe Bigfoot Spectral Cell Sorter is in a league of its own: upto 10x faster than the competition. Configurable—the high-end configurations of the Bigfoot Spectral Cell Sortertake advantage of the large number of lasers and detectors and allow spectralunmixing for both analysis and sorting Simple—the experiment wizard assists the user in running controls,identifying potential issues, and applying the unmixing algorithms to producehigh-quality data Flexible—the Bigfoot Spectral Cell Sorter allows both acquisition and sortingon spectrally unmixed data or conventionally compensated data, streamliningthe workflow from panel development to high-speed sorting Configurable—the Bigfoot Spectral Cell Sorter has userconfigurable sort output holders for 1.5, 5, 15, and 50 mLtubes, microwell plates up to 1,536 wells, microscopeslides, and even 10x chips with integrated temperaturecontrol (4–37 C) providing maximum versatility Virtual sorting—with standard six-way sorting and virtual18-way sorting, the Bigfoot Spectral Cell Sorter can beused to separate multiple sort populations—from a singlesample or different samples—for walk-away sorting Calibration—with built-in stream calibration and dropdelay as well as media detection, sort setup is simplifiedfor users4 High throughput—with sort rates 70,000 eps, sortingis fast and configurable. From four-way sorting into96‑well plates, eight-way sorting into 384-well plates, orstraight-down sorting into 1,536-well plates, the speedand recovery of the Bigfoot Spectral Cell Sorter shouldexceed your expectations. Integrated—from built-in media detection camerasto volume tracking of sorted samples, the integratedfeatures of the Bigfoot Spectral Cell Sorter reducecommon errors with cell sorting Jet-in-air—the Bigfoot Spectral Cell Sorter’s jet-in-airsensing, gentle with fragile cells, enables the usage of awide range of nozzle tip sizes while taking advantage ofhigh sort speed and yield5

BiosafetySample inputClass IIWith a custom-designed Class II biocontainment cabinet,the Bigfoot Spectral Cell Sorter enables both safety andsample protection, and is ergonomically optimized forsorting workflows. Adjustable—the adjustable sash maintains containmentwhile allowing easy access to the nozzle and sample/sortareas, providing constant user protection Ease of access—only parts such as the nozzle, sample,and sort chamber are enclosed; the electronics, lasers,and optics remain outside the containment zone,improving service access and temperature regulationEfficient 6 input positions—the Bigfoot Spectral Cell Sorter enables sampling from1.5, 5, and 15 mL tubes with automatic tube-type sensing and built-incrash detection Integrated wash station and on-board calibration beads—the integratedwash station reduces carryover, and on-board calibration beads allowprogrammable, automated start-up and calibration Built-in agitation and temperature control—using the built-in agitation andtemperature control (4–37 C), the Bigfoot Spectral Cell Sorter maintains theintegrity of your samples from start to finish Integrated—dedicated sort-area aerosol managementsystem (AMS), HEPA filter, and integrated softwarecommunication provide containment warnings andincreased flow to maintain protection if a clog is detected Organized—built-in sample vortex, plate storage area,tube rack, and biohazard bag streamline workflow Compliant—the system has been verified to befunctionally equivalent to the personnel and productprotection standards for a Class II Type A2 biosafetycabinet per NSF International Standard 49 Certified—an airflow/containment certificationprotocol is provided, giving guidance to your internalbiohood certifier67

NozzleElectronicsPowerfulWith custom electronics, firmware, and software designedspecifically for high-performance sorting, the BigfootSpectral Cell Sorter has the power and flexibility to tackleyour application challenges. Accurate—proprietary electronics simultaneously collecthigh dynamic range data for measured peak, area,and width for every channel to accurately characterizeyour sample High-end performance—the massively parallel,pipelined architecture eliminates hard aborts and allowscomplex, high-color experiments with up to a 60 x 60compensation matrix, or spectral unmixing withoutlimiting instrument performance Zero dead time—dynamic window extension ensuresfull data collection for every sample Automatic laser delay—without user interaction, theelectronics automatically configures the optimal laserdelay for different nozzle sizes and pressures Sort logic—flexible configuration allows for independentsort logic setups built from 64 total bivariate gates of upto 512 x 512 resolution, along with multiple modes forpurity, enrichment, and drop envelopesStable Gentle with fragile cells—jet-in-air sensing is gentlewith fragile cells, maximizing viability with a wide rangeof nozzle tip sizes while taking advantage of high sortspeeds and yield Simplified tip swapping—designed with ease of usein mind, the nozzle storage station and swap tip wizardsimplify the workflow and help reduce user errorsduring setup 5-axis automated stream alignment—ease of use isimproved and short- to long-term variability reduced withautomated 5-axis stream alignment and QC Reduced dead volume—built-in bubble detectionnotifies the user and automatically stops sample,reducing dead volume Precise droplet monitoring—integrated control systemsensure optimal droplet formation persistence andposition stabilization over time, ensuring accurate dropdelay maintenance through the day 50–150 µm nozzle tips—supports 50, 70, 100, 120, and150 µm nozzle tips with varying sheath pressures89

StreamlinedAdjustable monitor and keyboardarms provide ergonomically optimizedflexibility over multiple heightsand positions.Convenient storage is provided foradapters, spare parts, and tubes to helpreduce cluttered lab space.No external support componentsare needed. Goodbye externalwater baths, vacuum pumps,and compressors.Hot-swappable bulk-fluid bottlesallow for a full shift of operation, orfor continuous operation by changingduring a sort. Optional kits are availablefor connecting to house DI and waste toreduce the need to fill and empty bulkfluid tanks.On-board cleaner, decontamination, and sheathconcentrate bottles allow for automated sheathmixing as well as system rinsing and cleaningwithout manual intervention.1011

OpticsFlexibleThe optical platform of the Bigfoot Spectral Cell Sorteradapts to your needs with flexible laser options andoptimized filter sets. Flexible—the Bigfoot Spectral Cell Sorter offers freespace excitation of up to nine lasers into seven pinholesranging from 349 nm to 785 nm, allowing flexiblewavelength selection for your multicolor experiments Multiple scatter options—multiple scatter options,including small particle scatter detection anddepolarization, offer the ability to fully equip your systemfor your needs Smart—integrated detection-path checking is provided,with a broadband LED source to confirm detectionperformance and optical configuration Stable—integrated beam shaping and short path lengthsmaintain optical stability day to day Configurable—with up to 60 detectors, the BigfootSpectral Cell Sorter will adapt to your multicolorapplications while still allowing optical filter changes forfuture needs1213

SQSCustomer careIntuitiveSupportSQS utilizes a clean user interface with intuitive workflowand integrated features, allowing simplified acquisition,analysis, and sorting. System health—with multiple cameras and sensorsthroughout, the Bigfoot Spectral Cell Sorter providesfeedback on all subsystems and logs status forproactive troubleshooting Automated—start-up, quality control, and drop delayprocesses are all automated and let you program thesystem to be ready when you are Advanced fluorochrome selector—helps with designfor both standard and spectral experiments, and warnsof potential conflicts Remote monitoring—system health monitoring providesstatus and control, giving you the freedom to walk awayfrom the system while still monitoring experiments and tostart-up or shutdown remotely Launcher—along with ensuring the proper initializationand launch of SQS, the launcher also providesthe most secure method of installing and updatinginstrument software Remote support—cloud-based instrument reportingallows our knowledgeable team to support your systemwith the fastest response, increasing your uptime Email notifications—manage email notifications to helpsave time and streamline your workflow, helping you tosupport your end users High speed—Acquire and store files at 100,000 epsup to 100 million events per FCS file with all parametersenabled, reducing rare event detection time Full reporting capabilities—with reports suchas system usage, quality control, trending, andsort summary, information is presented clearly forfurther analysis Compatibility—generate FCS files that are compatiblewith other analysis software packages Analysis features—features including overlays, colorgating, back-gating, index sorting, and ratio plots giveyou added capability to display and publish data1415

Application dataApplication dataConsistent path lengths, stable optical filter layouts, and highly sensitive PMTdetectors optimize detection across the entire spectrum.Analysis of human bloodThe data below show a multicolor panel run on a nine‑laserBigfoot Spectral Cell Sorter. The panel shown definesT-cell subsets using lysed whole blood from a normaldonor. Antibodies used are CD45 FITC, CD3 Alexa Fluor 532, CD4 BUV395, CD8 APC, CD16 BV510, CD56 PE,8-peak bead resolution inkey channelsSpherotech 8-peak beads run onthe Bigfoot Spectral Cell Sorter showsignal resolution in key channels.CD19 PE-Cy5.5 , CD20 Alexa Fluor 700, CD25 PE-eFluor 610, CD127 BV421, TCRgd PerCP-eFluor 710, CD14 PECy7 , CD45RA BV711, and CD45RO BV786. The data areshown with spectral unmixing and biexponential scaling.BFPCascade Blue FITCPEPE-Cy5 PE-Texas Red APC2842131427101610010110210310410517