WIXLIB

Discover the Cutting EdgeCell SorterFrom Propel LabsTheCell Sorter with Sasquatch Software (SQS) provides thepower, safety, performance, and flexibility for your lab today and intothe future.With a custom designed, integrated Class II biocontainment cabinet,provides safety and protection without compromising highparameter sorter performance. Multi-tube input paired with 18-wayvirtual sorting with integrated temperature control gives flexibility forall your applications.With a stand-alone footprint and no ancillary requirements, high-speedelectronics enabling 100,000 eps acquisition and 70,000 eps sortingand hot-swap fluidics,provides unmatched performance inany lab space.can be configured with up to nine lasers and 60 detectors,providing the versatility for both standard fluorescence detection andspectral unmixing. Multiple scatter options allow simultaneousstandard and small particle detection, multi-laser scatter detection,and/or polarization.SQS provides quick start-up, automated calibration, and accurate qualitycontrol combined with an experiment designer, intuitive interface, andefficient shutdown allowing the system to be easy to use while reducingdowntime. Remote access capability, system health information, andemail notifications save time and streamline your workflow.

SpectralHigh ThroughputHigh SpeedConfigurable, quick, and calibrated. Bigfoot’s high throughput is in a league of its own: up to 10x fasterthan the competition. ConfigurableThe Bigfoot system has user configurable sortoutput holders for 1.5, 5, 15, and 50mL tubes,microwell plates up to 1536 wells, microscopeslides, and even 10X Chips* with integratedtemperature control (4-37 C) providingmaximum versatility. High-throughputWith sort rates up to 70,000 eps, sorting is fastand configurable. From 4-way sorting into 96well plates, 8-way sorting into 384 well platesor straight down sorting into 1536 well plates,Bigfoot speed and recovery will exceedyour expectations. Virtual sortingWith standard 6-way sorting and virtual 18-waysorting Bigfoot can be used to separate multiplesort populations from a single sample or differentsamples for walk away sorting. IntegratedFrom built-in media detection cameras to volumetracking of sorted samples, Bigfoot’s integratedfeatures reduce common errors with cell sorting. CalibrationWith built-in stream calibration and dropdelay as well as media detection, sort setupis simplified for users.Sorting andAnalysisThe first of its kind, Bigfoot allows bothspectral sorting and spectral analysis oncell populations. ConfigurableBigfoot high-end configurations take advantage of thelarge number of lasers and detectors and allow spectralunmixing for both analysis and sorting. SimpleThe experiment wizard assists the user in runningcontrols, identifying potential issues, and applying theunmixing algorithms to produce high quality data. FlexibleBigfoot allows both acquisition and sortingon spectrally unmixed data or conventionallycompensated data, streamlining workflow from paneldevelopment to high speed sorting for all your users. Jet-in-airBigfoot’s jet-in-air sensing, gentle with fragilecells, enables the usage of a wide range of nozzletip sizes while taking advantage of high sortspeed and yield.

BiosafetySample InputClass IIEfficientWith a custom-designed Class II biocontainment cabinet, Bigfoot provides both safety and sample protectionthat is ergonomically optimized for sorting workflow. 6 input positionsBigfoot enables sampling from 1.5, 5, and 15mL tubes with automatic tube type sensing and built-incrash detection. AdjustableThe adjustable sash maintains containment whileallowing easy access to the nozzle and sample/sortareas providing constant sample and user protection. Ease of accessEnclosing only parts such as the nozzle,sample and sort chamber; the electronics,lasers, and optics remain outside thecontainment zone improving service accessand temperature regulation. IntegratedDedicated sort area aerosol management(AMS) HEPA filter and integrated softwarecommunication provides containmentwarnings and increased flow if a clog isdetected to maintain protection. OrganizedBuilt in sample vortex, plate storagearea, tube rack, and biohazard bagstreamline workflow. CompliantThe system has been verified to meetpersonnel and product protectionstandards for a Class II Type A2biosafety cabinet per NSF InternationalStandard 49. CertifiedAn airflow/containment certificationprotocol is provided giving guidance toyour internal biohood certifier. Integrated wash station and on-board calibration beadsThe integrated wash station reduces carryover and on-board calibration beads allow programmable,automated startup and calibration. Built-in agitation and temperature controlUsing the built-in agitation and temperature control (4-37 C), Bigfoot maintains the integrity ofyour samples from start to finish.

PowerfulWith custom electronics, firmware, and software designed specifically for high-performance sorting,Bigfoot has the power and flexibility to tackle your application challenges. AccurateProprietary electronics simultaneously collecthigh-dynamic range data for measured peak,area, and width for every channel to accuratelycharacterize your sample. High-end performanceThe massively parallel, pipelined architectureeliminates hard aborts and allows complex,high color experiments with up to a 60 x 60compensation matrix or spectral unmixingwithout limiting instrument performance. Zero dead timeDynamic window extension ensures fulldata collection for every sample. Automatic laser delayWithout user interaction, the electronicsautomatically configures the optimal laser delayfor different nozzle sizes and pressures. Sort logicFlexible configuration allows for independent sortlogic setups built from 64 total bivariate gates ofup to 512 x 512 resolution, along with multiplemodes for purity, enrichment, and drop envelopes.Stable Gentle with fragile cellsBigfoot’s jet-in-air sensing is gentle with fragilecells, maximizing viability with a wide range ofnozzle tip sizes while taking advantage of highsort speeds and yield. 5-axis automated stream alignmentEase of use is improved and short to long termvariability reduced with automated 5-axis streamalignment and QC. Precise droplet monitoringIntegrated control systems ensure optimal dropletformation persistence and position stabilizationover time ensuring accurate drop delaymaintenance through the day.Nozzle Simplified tip swappingDesigned with ease of use in mind, the nozzlestorage station and swap tip wizard simplify theworkflow and reduce user errors during setup. Reduced dead volumeBuilt-in bubble detection notifies the userand automatically stops sample, reducingdead volume. 50-150µm nozzle tipsBigfoot supports 50, 70, 100, 120, and 150 µmnozzle tips.Electronics

Streamlined Optional, adjustable monitorand keyboard arms provideergonomically-optimizedflexibility over multipleheights and positions. Convenient storage isprovided for adapters, spareparts, and tubes to helpreduce cluttered lab space. No external support componentsare needed. Goodbye externalwater baths, vacuum pumps,and compressors. Hot-swappable bulk-fluidbottles allow for a full shift ofoperation, or for continuousoperation by changing duringa sort. Optional kits areavailable for connecting tohouse DI and waste to reducethe need to fill and emptybulk fluid tanks. On-board cleaner, decontamination,and sheath concentrate bottles allowfor automated sheath mixing as well assystem rinsing and cleaning withoutmanual intervention.

OpticsFlexibleThe Bigfoot optical platform adapts to your needs with flexible laser options and optimized filter sets. FlexibleBigfoot offers free space excitation of up to nine lasers into seven pinholes ranging from 349nm to785nm, allowing flexible wavelength selection for your multi-color experiments. StableIntegrated beam shaping and short path lengths maintain optical stability day to day. ConfigurableWith up to 60 detectors, Bigfoot will adapt to your multi-color applications while still allowingoptical filter changes for future needs. Multiple scatter optionsMultiple scatter options, including small particle scatter detection and polarization, give the abilityto fully equip your system for your needs. SmartIntegrated detection verification is provided with an LED array to confirm instrument performance andverify the optical configuration.

IntuitiveSupportSQS utilizes a clean user interface with intuitive workflow and integrated features allowingsimplified acquisition, analysis, and sorting. AutomatedStartup, quality control and drop delay processes areall automated and let you program the system to beready when you are. System healthWith multiple cameras and sensors throughout, Bigfootsystem health provides feedback on all subsystems and logsstatus for proactive troubleshooting. Advanced fluorochrome selectorHelps with experimental design for both standardand spectral experiments and warns of potential conflicts. Propel Labs launcherWhile ensuring the proper initialization and launching ofSQS the launcher also provides the most secure method ofinstalling and updating instrument software. Remote monitoringSystem health monitoring provides status and control,giving you the freedom to walk away from the systemwhile still monitoring experiments from your phone ortablet and to startup or shutdown remotely. High speedAcquire and store files at 100,000 events per second,up to 100 million events per FCS file with all parametersenabled reducing rare event detection time. Remote supportCloud-based instrument reporting allows our knowledgeableteam to support your system with the fastest response,increasing your up time. Email notificationsManage email notifications to save time and streamline yourworkflow – helping you support your end users. Full reporting capabilitiesWith reports such as system usage, quality control, andtrending and sort summary, information is presentedclearly for further analysis. CompatibilityBigfoot generates FCS files that are compatible withother analysis software packages. Analysis featuresFeatures including overlays, color-gating, back-gating,index sorting and ratio plots give you added capabilityto display and publish data.SQSCustomer Care

Application Data8-peak Bead Resolution in Key ChannelsSpherotech* 8-peak beads runon the Bigfoot Cell Sorter showsignal resolution in key channels.Consistent path lengths,stable optical filter layouts,and highly sensitive PMTdetectors optimize detectionacross the entire spectrum.Analysis of Human BloodData below shows a multi-color panel run on a 9-laserBigfoot Cell Sorter. The panel shown defines T-cellsubsets using lysed whole blood from a normal donor.Antibodies used are CD45 FITC, CD3 Alexa 532,CD4 BUV395, CD8 APC, CD16BV510, CD56PE, CD19 PE-Cy5.5, CD20 Alexa700, CD25 PEeFluor610, CD127 BV421, TCRgd PerCP-eFluor710,and CD14 PE-Cy7. The data is shown with spectralunmixing and biexponential scaling.BFPCascade BlueFITCPEPE-CY5PE-Texas RedAPC284213142710100101102103104105