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Prevail HPLC ColumnsWith Prevail Columns, You’ll Find aSolution for all Your HPLC Column Needs A General-Purpose Reversed-phase Columnfor a Wide Range of Applications Stability in Highly Aqueous Mobile Phases Excellent Retention of Non-polar Analytes A Polar-embedded Phase Specialty Phases for SpecificApplications Formats for Your LC/MSApplicationsBulletin #460AUNPContact your Alltech office or distributor for current or local prices. Copyright 2006 Alltech Associates, Inc.
Aqueous Mobile Phases . . .- Separate Highly Polar Analytes Without Fear of Phase Collapse Excellent Retention and Reproducibility ofHighly-Polar AnalytesUnderivatized Amino Acids5 No Phase CollapsePrevail HPLC columns offer exceptional versatility fordifficult separations, including highly-polar analytes on C18.Traditional reversed-phase packings experience bondedphase collapse under highly aqueous (95 %) conditions.Prevail bonded phases remain fully extended, even under100% aqueous conditions, for effective retention of highlypolar analytes without the detrimental effects of phasecollapse.94491. Glycine (Gly)2. Serine (Ser)3. Aspartic Acid (Asp)4. Glutamine (Gln)5. Alanine (Ala)6. Glutamic Acid (Glu)7. Lysine (Lys)7123460 1 2 3 4 5 6 7 8 9 10 Min.Column:Mobile Phase:Flow Rate:Detector:Prevail C18, 5µm, 250 x 4.6mm5mM Heptafluorobutyric Acid pH 1.0 w/ 0.7% TFA1.0mL/minELSDPrevail ’s Bonded Phases are Stable in 100% Aqueous Conditions9450Competitor A C18, 5µm, 150 x 4.6mmPrevail C18, 5µm, 150 x 4.6mm94519454Catecholamines19452111. Norepinephrine2. Epinephrine3. 5-Hydroxydopamine2945321945522333After 24 hrs.3After 8 hrs.After 20 min.0123456 Min.0123456 min.Mobile Phase: 25mM KH2PO4, pH 2.7Flow Rate:1.0mL/minDetector:UV at 270nmPrevail C18 remains stable and provides reproducible separations under highly aqueous conditions that cause traditional C18 phases to collapse.2TheProduct LinesContact your Alltech office or distributor for current or local prices. Copyright 2006 Alltech Associates, Inc.
Nucleic Acid BasesNucleosides941194101. Cytosine2. Uracil3. Guanine4. Adenine5. Thymine11. Cytidine2. Uridine3. Xanthine4. Guanosine5. Adenosine223551443024Column:Mobile Phase:Flow Rate:Detector:68 Min.024Column:Mobile Phase:Flow Rate:Detector:Prevail C18, 5µm, 150 x 4.6mm25mM KH2PO4 pH 3.0:CH3CN (98:2)1.0mL/minUV at 254nm Water Soluble Vitamin Standards68 Min.Prevail C18, 5µm, 150 x 4.6mm25mM KH2PO4 pH 3.0:CH3CN (96:4)1.0mL/minUV at 254nmWater Soluble VitaminsFrom Multi-Vitamin Tablet9388u945663251. Thiamine2. Ascorbic Acid3. Nicotinic Acid4. Fumaric Acid5. Pyridoxine6. Niacinamide7. Pantothenic Acidu. Unknown6710246810125214 Min.Column:Mobile Phase:Flow Rate:Detector:The10427468101214 Min.Prevail C18, 5µm, 150 x 4.6mm25mM KH2PO4, pH 3.0:CH3CN (97:3)1.0mL/minUV at 212nmProduct LinesContact your Alltech office or distributor for current or local prices.3 Copyright 2006 Alltech Associates, Inc.
Organic Mobile Phases . . .- Separate Hydrophobic Analytes with Excellent Retention and Peak Shape Strongly Retain Hydrophobic AnalytesVitamin E Soft Gel Tablet Eliminate Concerns about Solubility ofNon-Polar Compounds945832 Increase Sensitivity in LC/MS and ELSDApplicationsMany reversed-phase packings that tolerate highly aqueousconditions lack the hydrophobicity needed to retaincompounds under 100% organic mobile phase conditions.Prevail packing’s high carbon load retains hydrophobicanalytes even under 100% organic mobile phase conditions.Using 100% organic mobile phases increases the solubilityof highly hydrophobic compounds, eliminating analyteprecipitation. Removing the aqueous mobile phasecomponent increases sensitivity in LC/MS and ELSDapplications where the mobile phase volatility is critical.1. δ-Tocopherol2. γ-Tocopherol3. α-Tocopherol (Vitamin E)10 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Min.Column:Mobile Phase:Flow Rate:Detector:Prevail C18, 5µm, 150 x 4.6mmCH3CN:MeOH (75:25)1.5mL/minELSDFat-Soluble Vitamins and Tocopherols94579390Prevail C18, 5µm, 150 x 4.6mm651024610122, 3, 451786, 71. Vitamin A2. δ-Tocopherol3. γ-Tocopherol4. α-Tocopherol (Vitamin E)5. Vitamin D26. Vitamin D37. Vitamin K42 3Competitor B C18, 5µm, 150 x 4.6mm14 Min.Mobile Phase: CH3CN:MeOH (75:25)Flow Rate:1.5mL/minDetector:UV at 220nm02468101214 Min.Mobile Phase: CH3CN:MeOH (75:25)Flow Rate:1.5mL/minDetector:UV at 220nmPrevail C18’s hydrophobicity separates non-polar compounds under high organic mobile phase conditions where other C18 columns fail.4TheProduct LinesContact your Alltech office or distributor for current or local prices. Copyright 2006 Alltech Associates, Inc.
Polyaromatic HydrocarbonsHydrocortisone Acetate Cream9391394631. Chrysene2. Benzo[k]fluoranthene3. Indeno[1,2,3,c,d]pyrene11. Methyl Paraben2. Propyl Paraben3. Hydrocortisone Acetate1232024Column:Mobile Phase:Flow Rate:Detector:6810 Min.0Prevail C18, 5µm, 150 x 4.6mmCH3CN:MeOH (75:25)1.0mL/minUV at 254nm12Column:Mobile Phase:Flow Rate:Detector:35 Min.Prevail C18, 5µm, 150 x 4.6mmCH3CN:H2O (90:10)1.0mL/minUV at 254nmLong Chain Fatty Acids34Steroids4945993921. Capric Acid (C10)2. Lauric Acid (C12)3. Myristic Acid (C14)4. Palmitic Acid (C16)5. Stearic Acid (C18)51. Hydrocortisone2. Testosterone3. Progesterone1232101Column:Mobile Phase:Flow Rate:Detector:234 Min.Prevail C18, 3µm, 53 x 7mm, RocketCH3CN:MeOH (75:25)3.5mL/minELSD 0 Column:Mobile Phase:Flow Rate:Detector:1 Min.Prevail C18, 3µm, 53 x 7mm, Rocket MeOH:H2O (90:10)4.5mL/minUV at 254nmUse Prevail Rocket columns for fast analyses without degrading the separation.TheProduct LinesContact your Alltech office or distributor for current or local prices.5 Copyright 2006 Alltech Associates, Inc.
. . . and Everything in Between- The Most Versatile Choice for Your HPLC Column 7 810 24631. Neomycin2. Amoxicillin3. Vancomycin4. Cefatrizine5. Metampacillin6. Cefotaxime7. Unknown8. Bacitracin9. Pipericillin10. Bacampacillin11. Oxacillin4108 10 12 14 16 18 20 22 Min.Column:Prevail C18, 5µm, 150 x 4.6mmMobile Phase: A: 25mM KH2PO4 pH 3.0B: CH3CNGradient:Time: 0 15 22%B: 10 40 40Flow Rate:1.0mL/minDetector:UV at 210nm24261. Uracil2. Phenol3. 4-Phenylbutyric Acid4. N,N-Diethyl-m-Toluamide5. Propylbenzene6. Quinizarin7. Butylbenzene1223456Column:Mobile Phase:Flow Rate:Detector:6The94069395318 Min.Acids, Chelates, & Neutrals Mix1. Metoprolol2. Oxprenolol3. Propranolol01. Ethotoin2. Mephenytoin3. Carbamazepine4. DiazepamColumn:Prevail Phenyl, 5µm, 150 x 4.6mmMobile Phase: A: H2O B: CH3CNGradient:Time: 0 15%B: 25 60Flow Rate:1.5mL/minDetector:UV at 254nmAnti-hypertensives193942 Min.Prevail C18, 3µm, 53 x 7mm, Rocket 25mM KH2PO4 pH 3.0:CH3CN (70:30)4.0mL/minUV at 220nm0246Column:Mobile Phase:Flow Rate:Detector:8710 12 14 16 Min.Prevail C18, 5µm, 150 x 4.6mm25mM KH2PO4 pH 2.5:CH3CN (35:65)1.0mL/minUV at 254nmProduct LinesContact your Alltech office or distributor for current or local prices. Copyright 2006 Alltech Associates, Inc.
Polyphenolic Flavonoids426939831. Myricetin2. Quercetin3. Naringenin4. Hesperetin5. Apigenin6. Kaempferol31Aromatics939751. Uracil2. Phenol3. Toluene4. Ethylbenzene2410 2 46 8 10 12 14 16 18 20 22 24 26 Min.0Column:Prevail C18, 5µm, 150 x 4.6mmMobile Phase: A: 25mM KH2PO4 pH 2.5B: CH3CNGradient:Time: 0 15 26%B: 20 40 40Flow Rate:1.0mL/minDetector:UV at 280nm2Column:Mobile Phase:Flow Rate:Detector:Peptides46 Min.Prevail Phenyl, 5µm, 150 x 4.6mmH2O:CH3CN (50:50)1.0mL/minUV at 254nmPolyaromatic Hydrocarbons93995. Leucine Enkephalin6. Oxytocin7. Angiotensin II8. Substance P1. GLY-TYR2. VAL-TYR-VAL3. Methionine Enkephalin4. Physalemin3452194001. Benzene2. Naphthalene3. Acenaphthylene4. Fluorene5. Acenaphthene6. Phenanthrene7. Anthracene8. Fluoranthene9. Pyrene761146 75881 2302468101214 Min.Column:Prevail C18, 5µm, 150 x 4.6mmMobile Phase: A: 0.15%TFA in H2OB: 0.13% TFA in CH3CN:H2O (95:5)Gradient:Time: 0 15%B: 20 55Flow Rate:1.0mL/minDetector:UV at 220nmThe026121413910410. 1,2-Dibenzanthracene11. Chrysene12. Benzo[b]fluoranthene13. Benzo[k]fluoranthene14. Benzo[a]perylene15. 1,2,5,6-Dibenzanthracene16. 1,2-Dibenzoperylene17. Indeno[1,2,3,c,d]pyrene815161710 12 14 16 Min.Column:Prevail C18, 5µm, 150 x 4.6mmMobile Phase: A: H2OB: CH3CNGradient:Time: 0 4 15%B: 72 72 100Flow Rate:1.5mL/minDetector:UV at 254nmProduct LinesContact your Alltech office or distributor for current or local prices.7 Copyright 2006 Alltech Associates, Inc.
Prevail Amide Columns- A Polar-Embedded Phase Improves Peak Shape for Polar Analytes in NeutralpH Mobile Phases Polar-Embedded Group ImprovesPolar Sample Peak ShapeAnalgesics9385 Packing Media Provides Unique Selectivity1. Aspirin2. Acetaminophen3. Naproxen4. Fenprofen Bonded Phase does not Collapse with 100%Aqueous Mobile Phases1A polar-embedded group inserted into the hydrophobic chainof the Prevail Amide phase minimizes interaction of polarsamples with silanols, providing symmetrical peaks for a widevariety of applications. The amide phase is especially useful atneutral pH where amines can interact strongly with ionizedsilanols. The presence of the amide group alters the selectivity of the reversed-phase packing, giving separations thatare often hard to achieve on conventional reversed-phasecolumns.230The polar-embedded group also helps to wet the hydrophobic chains, and prevents phase collapse in highly aqueousmobile phases.2 4 648 10 12 14 Min.Column:Mobile Phase:Flow Rate:Detector:Prevail Amide, 5µm, 150 x 4.6mm20mM K2HPO4, pH7.0:CH3CN (75:25)1.0mL/minUV at 210nmAnesthetics1Anilines9436941221. Aniline2. Dimethylaniline3. Diethylaniline1. p-Aminobenzoic Acid2. Benzocaine3. Butacaine4. Tetracaine123024Column:Mobile Phase:Flow Rate:Detector:8The34681012 Min.Prevail Amide, 3µm, 150 x 4.6mm20mM K2HPO4, pH 7.0:CH3CN (43:57)1.0mL/minUV at 210nm024Column:Mobile Phase:Flow Rate:Detector:6810 Min.Prevail Amide, 5µm, 150 x 4.6mm20mM K2HPO4, pH7.0:CH3CN (50:50)1.0mL/minUV at 210nmProduct LinesContact your Alltech office or distributor for current or local prices. Copyright 2006 Alltech Associates, Inc.
Prevail Organic Acid Columns- A New Standard for Organic Acid Analysis Silica-Based Column for Maximum Efficiencyand High ResolutionOrganic Acids in Rosé Wine9414 Short Run Times and High Sample Throughput atAmbient Temperature1. Tartaric Acid2. Malic Acid3. Lactic Acid4. Acetic Acid5. Succinic Acid Acid Stable Media for Long Column Lifetimes Lower Cost than Polymeric Organic Acid Columns1Prevail Organic Acid (OA) columns separate commonorganic acids with an unsurpassed combination of resolution,speed, sensitivity, and simplicity. A simple acidic phosphatebuffer and a Prevail OA column at ambient temperature willseparate 11 short-chain organic acids in less than 6 minutes.Need more resolution? Try the 250 x 4.6mm Prevail OAcolumn. Want faster analyses? Choose the Prevail OrganicAcid Rocket column.Use pH to adjust column selectivity. Lowering the mobilephase pH progressively suppresses the ionization of the carboxylic acids, making them more hydrophobic. This gives youthe ability to move these peaks relative to other peaks in thechromatogram, and it simplifies method development.2340246Column:Mobile Phase:Flow Rate:Detector:Organic Acid Standards58101214 Min.Prevail Organic Acid, 5µm, 150 x 4.6mm25mM KH2PO4, pH 2.50.7mL/minUV at 210nmOrganic Acids in Orange mn:Mobile Phase:Flow Rate:Detector:The53494781. Malic Acid2. Ascorbic Acid3. Citric AcidOxalic AcidTartaric AcidFormic AcidMalic AcidLactic AcidAcetic AcidDiglycollic AcidMaleic AcidCitric AcidSuccinic AcidFumaric Acid321105678 Min.Prevail Organic Acid, 5µm, 150 x 4.6mm25mM KH2PO4, pH 2.51.5mL/minUV at 210nm02Column:Mobile Phase:Flow Rate:Detector:46810 Min.Prevail Organic Acid, 5µm, 150 x 4.6mm25mM KH2PO4, pH 2.51.0mL/minUV at 210nmProduct LinesContact your Alltech office or distributor for current or local prices.9 Copyright 2006 Alltech Associates, Inc.
Prevail Carbohydrate ES Columns– A Superior Solution for Carbohydrate Analysis Longer Column Lifetime than Traditional AminoColumnsSugar Alcohols More Versatile Than Ion Exclusion Columns9352 Optimum Resolution and Peak Shapes at Ambient Temperature1. Iso-erythritol2. Xylitol3. Mannitol4. Maltitol1 2 Range of Useful Formats Combine with Alltech’s ELSD for the Carbohydrate Solution: Enhanced Detector Sensitivity,Flat Baselines, Gradient Capability, and ReducedRun TimesPrevail Carbohydrate ES offers performance superiorto traditional amino columns and ion exclusion columns.Because of its rugged bonding technology, Carbohydrate ESdelivers quiet, stable baselines and excellent peak shapes longafter silica-based amino columns have died. Carbohydrate EScan also separate all carbohydrate classes from mono- to oligosaccharides and complex carbohydrates, making it superiorin versatility to application-specific ion exclusion columns.3024Column:Mobile Phase:Flow Rate:Detector:This column’s full potential is realized when used in conjunction with the Evaporative Light Scattering Detection (ELSD).Gradients are powerful and effective when done with theCarbohydrate ES Column/ELSD combination. With gradients, run times are reduced, peak efficiencies are maximized,and detector sensitivity is enhanced.4681012 Min.Prevail Carbohydrate ES, 250 x 4.6mmCH3CN:H2O (70:30)1.0mL/minELSD1V8 Vegetable Juice9485Prevail Carbohydrate ES is a 5µm spherical packing offeredin four hardware formats: 250 x 4.6mm; 150 x 4.6mm; 100 x7mm Rocket ; 53 x 7mm Rocket . For the most complexsamples, use the 250 x 4.6mm or 100 x 7mm columns. Forsimpler mixtures and faster chromatography, consider usingthe 53 x 7mm Rocket format. Both 7mm i.d. Rocket formats offer the advantages of reduced backpressures andfaster separations.1. Mannitol2. Fructose3. Glucose4. Sucrose34202468 Min.Column:Prevail Carbohydrate ES, 5µm, 100 x 7mm, Rocket Mobile Phase: CH3CN:H2O (80:20)Flow Rate:2.0mL/minDetector:ELSD10TheProduct LinesContact your Alltech office or distributor for current or local prices. Copyright 2006 Alltech Associates, Inc.
Sweetened Vending Machine CoffeeImported Ale94771. Dextrose2. Maltose3. Maltotriose4. Maltotetraose5. Maltopentaose6. Maltohexaose7. Maltoheptaose3293514145 670241268 Min.0Column:Prevail Carbohydrate ES, 53 x 7mm, Rocket Mobile Phase: A: CH3CNB: H2OGradientTime: 0 4%B: 20 50Flow Rate:2.0mL/minDetector:ELSDColumn:Prevail Carbohydrate ES, 250 x 4.6mmMobile Phase: A: CH3CNB: H2OGradientTime: 0 50%B: 20 35Flow Rate:1.0mL/minDetector:ELSDComponents Frequently Foundin Fermentation Broths9417245935711. Mannitol2. Fructose3. Glucose4. Sucrose5. Raffinose6. Stachyose323456789 10 11 12 Min.Column:Prevail Carbohydrate ES, 100 x 7mm, Rocket Mobile Phase: A: CH3CNB: H2OGradient:Time: 0 10%B: 20 50Flow Rate:2.0mL/minDetector:ELSDThe1. Iso-erythritol2. Arabitol3. Mannitol4. Sorbitol5. Fructose6. Glucose7. Sucrose8. Maltitol9. Maltose10. Raffinose11. Maltotriose12. Stachyose62 430 15 6710 15 20 25 30 35 40 45 50 Min.5Simple Sugars11. Fructose2. Glucose3. Maltose4. Maltotriose5. Maltopentaose6. Maltohexaose7. Maltoheptaose30567 8910 11125 10 15 20 25 30 35 40 45 50 55 Min.Column:Prevail Carbohydrate ES, 250 x 4.6mmMobile Phase: A: CH3CNB: H2OGradient:Time: 0 60%B: 17 35Flow Rate:1.0mL/minDetector:ELSDProduct LinesContact your Alltech office or distributor for current or local prices.11 Copyright 2006 Alltech Associates, Inc.
Prevail LC/MS Columns- High Efficiency, No-Bleed LC/MS Columns High Resolving PowerFast Gradient No-bleed Packings9460 Stable Chemistry1.2.3.4.5.6.7.8.9. Variety of FormatsShort-chain organic acids (formic, acetic, trifluoroacetic) andtheir amine salts are often used as mobile phase modifiersin LC/MS systems because of their high volatility, leading toenhanced detector sensitivity. Alltech introduces Prevail LC/MS columns, which are exceedingly stable in thesepopular mobile phases. Prevail LC/MS columns have beentested under rigorous pH 1.0 conditions to ensure detectorbaseline stability by eliminating column bleed.Prevail bonded phases use high purity, small particle silica.The highly stable bonding chemistry provides high efficiency,long column life and better chromatography through reduced silanol interaction. This results in increased sensitivityand high resolution, which delivers a more concentrated LCfraction to the MS interface.Prevail columns offer the greatest mobile phase versatility,using 100% aqueous to 100% organic mobile phases withoutphase collapse. This material excels under a full spectrum ofmobile phase conditions, including stability to pH1.0.451736 anophenone20123 Min.Column:Prevail C18, 3µm, 20 x 2.1mm, Expedite Mobile Phase: A: 0.1% TFA in H2OB: 0.08% TFA in CH3CNGradient:Time: 0 2 3%B:0 100 100Flow Rate:1.0mL/minDetector:UV at s94451. Aspirin2. Acetaminophen3. Naproxen4. Diclofenac5. Indomethane6. Ibuprofen532194441. Hydrocortisone2. Cortisone124601234 Min.Column:Prevail C18, 3µm, 50 x 2.1mmMobile Phase: 50mM Ammonium Acetate, pH 5.4:CH3CN(60:40)Flow Rate:0.3mL/minDetector:UV at 254nm12The012 Min.Column:Prevail C18, 3µm, 50 x 2.1mmMobile Phase: 50mM Ammonium Acetate, pH 5.4:CH3CN (45:55)Flow Rate:0.3mL/minDetector:UV at 230nmProduct LinesContact your Alltech office or distributor for current or local prices. Copyright 2006 Alltech Associates, Inc.
Diet ColaBeverage Additives9447944651. Ascorbic Acid2. Saccharin3. Benzoic Acid4. Aspartame5. Vanillin21. Ascorbic Acid2. Citric Acid3. Benzoic Acid4. Aspartame5. Caffeine323511440103 Min.213 Min.2Column:Prevail C18, 3µm, 50 x 2.1mmMobile Phase: 50mM Ammonium Acetate, pH 5.4:CH3CN (80:20)Flow Rate:0.3mL/minDetector:UV at 230nmFat Soluble nMetampicillinVancomycinOxacillin5024421810 Min.Column:Prevail C18, 3µm, 50 x 2.1mmMobile Phase: A: 50mM Ammonium Acetate pH 5.4B: CH3CNGradient:Time: 0 8 10%B: 0 40 40Flow Rate:0.3mL/minDetector:UV at 220nmThe1564231. Vitamin A degradant2. Vitamin A3. Vitamin K degradant4. Vitamin E5. Vitamin K301Column:Mobile Phase:Flow Rate:Detector:23 Min.Prevail C18, 3µm, 50 x 2.1mmMeOH0.5mL/minUV at 220nmProduct LinesContact your Alltech office or distributor for current or local prices.13 Copyright 2006 Alltech Associates, Inc.
Exceptional Stability . . .- Long Column Life Even Under Extreme ConditionsStability StudyTricyclic Antidepressants938294089383InitialSeparation1. Uracil2. Phenol3. N, N-Diethylm-Toluamide4. Toluene24319409After 500 Hoursat pH 1.0 and 60 C2Run TimeandMobile PhaseConsumptionReduced by 50%12411. Doxepin2. Nortriptyline3. Trimipramine23133pH 4.2pH 1.0 with H2SO40 1 2 3 4 5 6 7 Min.Column:Mobile Phase:Flow Rate:Detector:0 1 2 3 4 5 6 7 Min.02468 10 12 14 16 18 20 22 24 Min.Column:Mobile Phase:Flow Rate:Detector:Prevail C18, 5µm, 150 x 4.6mmCH3CN:H2O (58:42)1.0mL/minUV at 254nm A Prevail C18 Column was washed for 500 hours at 60 C with anacetonitrile:water mobile phase adjusted to pH 1.0 with sulfuric acid.The column showed no chromatographic change.Prevail C18, 5µm, 150 x 4.6mm25mM KH2PO4:CH3CN (65:35)1.0mL/minUV at 210nmTake advantage of the Prevail Column’s stability by analyzing aminesat lower pH to reduce the analysis time. The analysis done at pH 1.0 iscompleted in half the time.Switch Between 100% Aqueous and 100% Organic Mobile Phases On the Same ColumnFat Soluble Vitamins and TocopherolsWater Soluble Vitamins1. Thiamine2. Ascorbic Acid3. Nicotinic Acid4. Pyridoxine5. Niacinamide6. Pantothenic Acid32421. Vitamin A2. δ-Tocopherol3. γ-Tocopherol4. α-Tocopherol(Vitamin E)4Column:6810 12 14 Min.Prevail C18, 5µm,150 x 4.6mmMobile Phase: 25mM KH2PO4 pH 3.0:CH3CN (97:3)Flow Rate:1.0mL/minDetector:UV at 212nmFigure 123wash withintermediate5462781. Thiamine2. Ascorbic Acid3. Nicotinic Acid4. Pyridoxine5. Niacinamide6. Pantothenic Acid642 30(100% Aqueous)94055. Vitamin D26. Vitamin D37. Vitamin K16109465wash withintermediate5Water Soluble Vitamins(100% Organic)(100% Aqueous)10Column:5611214 Min.Prevail C18, 5µm,150 x 4.6mmMobile Phase: CH3CN:MeOH (75:25)Flow Rate:1.5mL/minDetector:UV at 220nmFigure 2409466246810 12 14 Min.Column:Prevail C18, 5µm,150 x 4.6mmMobile Phase: 25mM KH2PO4 pH3.0:CH3CN (97:3)Flow Rate:1.0mL/minDetector:UV at 212nmFigure 3Prevail columns have highly stable bonded phases that let you use one column for multiple mobile phase conditions. Evenswitching between extremes such as 100% aqueous (Figure 1) to 100% organic conditions (Figure 2), and back to 100%aqueous (Figure 3) on a routine basis is possible, provided that mobile phases are miscible.14TheProduct LinesContact your Alltech office or distributor for current or local prices. Copyright 2006 Alltech Associates, Inc.
Prevail Analytical ColumnsPrevail LC/MS ColumnsPrevail ColumnsPrevail LC/MS ColumnsPACKINGPARTICLE SIZEC18LENGTH X I.D.PART NO.PACKINGC183µm150 x 2.1mm992003µm3µm3µm5µm5µm5µm50 x 4.6mm100 x 4.6mm150 x 4.6mm150 x 2.1mm150 x 4.6mm250 x m5µm5µm5µm150 x 2.1mm50 x 4.6mm100 x 4.6mm150 x 4.6mm150 x 2.1mm150 x 4.6mm250 x �m3µm5µm5µm5µm150 x 2.1mm50 x 4.6mm100 x 4.6mm150 x 4.6mm150 x 2.1mm150 x 4.6mm250 x µm3µm3µm5µm5µm5µm150 x 2.1mm50 x 4.6mm100 x 4.6mm150 x 4.6mm150 x 2.1mm150 x 4.6mm250 x µm3µm3µm5µm5µm5µm150 x 2.1mm50 x 4.6mm100 x 4.6mm150 x 4.6mm150 x 2.1mm150 x 4.6mm250 x 3µm3µm3µm5µm5µm5µm150 x 2.1mm50 x 4.6mm100 x 4.6mm150 x 4.6mm150 x 2.1mm150 x 4.6mm250 x �m3µm150 x 4.6mm250 x 4.6mm100 x 4.6mm150 x 4.6mm886408864588650886555µm5µm3µm3µm150 x 4.6mm250 x 4.6mm100 x 4.6mm150 x 4.6mm5µm5µm150 x 4.6mm250 x 4.6mmC8PhenylOAAmidePARTICLE SIZEExpedite Expedite Expedite Expedite PhenylExpedite Expedite Expedite Expedite SilicaExpedite Expedite Expedite Expedite LENGTH X I.D.PART NO.3µm3µm3µm3µm3µm3µm3µm3µm150 x 2.1mm100 x 2.1mm50 x 2.1mm20 x 2.1mm10 x 2.1mm20 x 4.6mm10 x 4.6mm33 x µm3µm3µm3µm3µm3µm3µm150 x 2.1mm100 x 2.1mm50 x 2.1mm20 x 2.1mm10 x 2.1mm20 x 4.6mm10 x 4.6mm33 x µm3µm3µm3µm3µm3µm3µm150 x 2.1mm100 x 2.1mm50 x 2.1mm20 x 2.1mm10 x 2.1mm20 x 4.6mm10 x 4.6mm33 x il High Speed Rocket ColumnsPrevail Rocket ColumnsPACKINGPARTICLE SIZELENGTH X I.D.PART NO.C183µm3µm53 x 7.0mm33 x 7.0mm9927999280Phenyl3µm3µm53 x 7.0mm33 x 7.0mm9928199282Silica3µm3µm53 x 7.0mm33 x 7.0mm992839928488660886658867088675OA3µm3µm53 x 7.0mm33 x 7.0mm5075599292Amide3µm3µm53 x 7.0mm33 x 7.0mm50775992983510235101Carbohydrate ES5µm5µm53 x 7.0mm100 x 7.0mm3510435103Carbohydrate ES Copyright 2006 Alltech Associates, Inc. Alltech Associates Inc., is a wholly owned subsidiary of W. R. Grace & Co.-Conn. Grace Davison is a business unit of W. R. Grace & Co.-Conn., Global Headquarters 7500 Grace Drive, Columbia, MD 21044The information presented herein is derived from our testing and experience. It is offered, free of charge, for your consideration, investigation and verification. Since operating conditions vary significantly, and since they are not under our control, we disclaim any and allwarranties on the results which might be obtained from the use of our products. You should make no assumption that all safety or environmental protection measures are indicated or that other measures may not be required.Alltech reserves the right to change prices and/orspecifications without prior notification. Printed in the USA.TheProduct LinesContact your Alltech office or distributor for current or local prices.15 Copyright 2006 Alltech Associates, Inc.
Expedite LC/MS Hardwarefor Fast, Low-Volume ApplicationsProtect Your Prevail Investment withAll-Guard CartridgesExpedite hardware is available in10 or 20mm lengths, and 2.1 or4.6mm i.d. Simple - Fewer Parts,Fewer Seals, FewerProblemsActual Size Fast - ChangeCartridges in SecondsWithout Tools20mm Reliable - Pressure and ChromatographicallyTested with Test Certificate Supplied Efficient - Matched Media & Low Dead VolumeMaintain Chromatographic Performance10mmPrevail All-Guard Cartridges*Expedite your LC/MS analyses with Alltech’s high-speed,low-volume Expedite hardware. These fast analysis columnspacked with high resolution Prevail media increase laboratory efficiency by doubling or even tripling sample throughput.See Page 12,13 & 15 for our full line of Prevail LC/MS ColumnsPACKINGPARTICLE SIZELENGTH X I.D.C185µm7.5 x 4.6mmC85µm7.5 x 4.6mmPhenyl5µm7.5 x 4.6mmCyano5µm7.5 x 4.6mmAmino5µm7.5 x 4.6mmSilica5µm7.5 x 4.6mmOA5µm7.5 x 4.6mmAmide5µm7.5 x 4.6mmAll-Guard Guard Cartridge HolderQTY.PART 4380101*Requires Guard HolderALLTECH , ALLTIMA , EVEREST , GROM , JONES CHROMATOGRAPHY , MODCOL , PLATINUM , PROSPHERE , and VYDAC are trademarks of Alltech Associates, Inc. DAVISIL , DAVISON ,GRACE , and GRACE DAVISON are registered trademarks of W. R. Grace & Co.-Conn. V8 is a registered trademark of Campbell Soup Company. This trademark list has been compiled using available published information as of the publication date of this brochure and may not accurately reflect current trademark ownership.2051 Waukegan Rd Deerfield, IL 60015Tel: 847-948-8600 Fax: 847-948-047701/06 #460ATheProduct LinesContact your Alltech office or distributor for current or local prices. Copyright 2006 Alltech Associates, Inc.
Column: Prevail Amide, 3µm, 150 x 4.6mm Mobile Phase: 20mM K2HPO4, pH 7.0:CHCN (43:57) Flow Rate: 1.0mL/min Detector: UV at 210nm 1. p-Aminobenzoic Acid 2. Benzocaine 3. Butacaine 4. Tetracaine Anesthetics 4 Prevail
