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HPLC ColumnsHPLC Columns & Bulk ResinsTHE MEASURE OF EXCELLENCETM

The Measure of ExcellenceHamiltonFor nearly 50 years, Hamilton Company has been satisfying customer needs in the field of precisionfluid measuring. It all started with syringes. Not commercial, mass produced medical syringes, butprecision measuring instruments. About 25 years ago Hamilton was the first company to develop andmanufacture pressure stable polymeric HPLC columns.ColumnsWe now offer 19 different polymer-based HPLC columns for reversed phase, anion exchange, cationexchange and ion exclusion separations. Two silica-based C8 and C18 columns are availablefor reversed phase separations.Choosing the Right HPLC ColumnBefore you select an HPLC column to separate your sample please check the Hamilton CompanyHPLC web site at www.hamiltoncompany.com/hplc/app index 1.aspor e-mail chromatography@hamiltoncompany.com for a complete list of compounds that havebeen separated on Hamilton HPLC columns.1

This brochure only contains a few of the applications which have been performed onHamilton HPLC columns. In the brochure, columns are grouped by separation mechanism:reversed phase, anion exchange, cation exchange and ion exclusion.The Recommended Uses Table below lists a few of the possible uses for each of thecolumn packings.Recommended UsesReversed PhasePRP -h1Long life column for LC/MS applications,synthesized DNA,and small moleculePRP-h5Reduced system pressure for protein and peptide separationsand enhanced oligonucleotide recoveryPRP-1General purpose pH stable long life column, synthesized DNAPRP-3Gradient protein and peptide separationsPRP-InfinityNonporous support for very fast gradient separation of large proteinsHxSil C8, C18General purpose silica-based reversed phaseAnion ExchangePRP-X100Anions, inorganic and organic using conductivity or UV detection.0 to 100% solvent compatible.PRP-X110Similar to PRP-X100 but for lower level anions (20 ppb to 20 ppm)PRP-X500Gradient separation of large proteins and labeled DNAPRP-X600Gradient separation of labeled and unlabeled DNARCX-10Isocratic or gradient separation of carbohydrate oligomers up to DP8RCX-30Gradient separation of complex carbohydratesCation ExchangePRP-X200Inorganic and organic cations using conductivity or UV detection.Separate mono or divalent cations depending on mobile phase conditions.PRP-X400Glyphosate and metabolite in drinking water. Also unique hydrophilicinteraction separationsPRP-X800Mono and divalent cations in the same run. Transition metalsHC-40Sugar oligomers up to DP8. Max pressure 1,000 psiHC-75Mono and disaccharides in corn syrup. Max pressure 1,000 psiCalcium FormHC-75Organic acids and sugars. Max pressure 1,000 psiHydrogen FormHC-75Sugar alcohols. Max pressure 1,000 psiLead FormIon ExclusionOrganic acids and alcoholsPRP-X3002

USP “L” Numbers & Bulk ResinsUSP "L" Number and Hamilton HPLC ColumnsUSP "L" NumberHAMILTON ColumnL1Octadecylsilane chemically bonded to porous silica or ceramicmicro-particles, 3 to 10 µm in diameter.HxSil C18L7Octylsilane chemically bonded to porous silica particles, 3 to 10 µm in diameter.HxSil C8L17Strong cation-exchange resin consisting of sulfonated cross-linkedstyrene-divinylbenzene copolymer in the hydrogen form, 7 to 11 µm in diameter.HC-75 Hydrogen FormL19Strong cation-exchange resin consisting of sulfonated cross-linkedstyrene-divinylbenzene copolymer in the calcium form, about 9 µm in diameter.HC-75 Calcium FormL21A rigid, spherical styrene-divinylbenzene copolymer, 5 to 10 µm in diameter.PRP-1PRP-3L23An anion-exchange resin made of porous polymethacrylate or polyacrylate gelwith quaternary ammonium groups, about 10 µm in size.PRP-X500L34Strong cation-exchange resin consisting of sulfonated cross-linkedstyrene-divinylbenzene copolymer in the lead form, about 9 µm in diameter.HC-75 Lead FormBulk eCapacityPoreSizePRP-h1PSDVB*N/A100 Å79279PRP-h5PSDVB*N/A300 Å79280PRP-1PSDVB*N/A100 ÅPRP-3PSDVB*N/A300 ÅHxSil C8SilicaHxSil ngeCapacityPRP-X100PSDVB* with Trimethylammoniun Exchanger0.19 meq/gm 100 ÅPRP-X500Poly(methacryl1.6 meq/gmamidopropryl Trimethylammonium Poly(dimethyl1.6 rialExchangeCapacityPoreSizePRP-X200PSDVB* with SulfonateExchanger35 µeq/gm100 ÅPRP-X400PSDVB* with SulfonateExchanger2.5 meq/gmN/A* PSDVB is Poly(styrene-divinylbenzene)Bulk resin is sold by the gram.PoreSize3 µm5 µm795787 µm7957910 µm7958012-20 µm 30-50 µm 50-75µm795817958279701797027958371942 79143 7914479139 79140791413 µm7 µm10 µm12-20 µm 30-50 µm 50-75µm79585795865 µm795843 µm5 µm7 µm7959110 µm12-20 µm 30-50 µm 50-75µm79587795887959279593

PRP -1 PhaseReversedHPLC ColumnsReversedHPLCPhaseColumnsPRP -h1Polymeric 100 Å columns for reversed phase, LC/MS applications. Low bleed (ideal for mass spectrometry). pH stable from 1 to 13. Robust (virtually any HPLC solvent can be used).Benzoic Acid and its Derivatives One particle size: 5 µm. Four column diameters: 2.1, 4.6, 10and 100 mm. Analytical and semiprep/preparativeguard columns.Naproxen SodiumPRP-h1, 5 µ, 100 Å, 4.1 x 50 mm24131PRP-h1, 5 µm, 100 Å,4.1 x 50 mm,1. 2,4-DihdroxybenzoicAcid2. 4-HydroxybenzoicAcid3. 2-AcetoxybenzoicAcid (Aspirin)4. Benzoic Acid5. 2- HydroxybenzoicAcid (Salicylic Acid)1. Naproxen (110 µg/mL)2. Ibuprofen (830 µg/mL)25050 mm100 mm150 mm2.1 mm79250792494.6 mm792527925379255792667925110 mm4Minutes6810Mobil Phase: A: 10 mmol/L sodiumdihydrogenphosphatepH 2.2 B: Acetonitrile. Gradient: 18% B (Isocratic)Flow Rate:0.60 mL/min. Column Temperature: 50 oCDetection: UV @230 nm. Injection Volumn: 5 µLOrdering InformationHPLC ColumnsI.D.2100 mm250 mm79256012Minutes345Mobil Phase: A: 10 mmol/L SodiumdihydrogenphosphatepH 2 B: Acetonitrile. Gradient: Isocratic 50% A/50%BFlow Rate:0.60 mL/min. Column Tempera ture: 60 oCDetection: UV @230 nm.Analytical Guard ColumnsFor Steel Columns*Starter Kit79257(1 holder, 2 cartridges)Replacement Cartridges (5/pk)79258* Analytical guard column for steel columns is 2.3 x 20 mm79523Semiprep/Preparative Guard ColumnStarter Kit(1 holder, 1 cartridge)Replacement Cartridges (2/pk)For Steel Columns***7927579276*** Semiprep/preparative guard column for steel columns is 4.6 x 20 mm4

PRP-h5 Reversed Phase HPLC ColumnsPolymeric 300 Å columns for oligonucleotide purification and protien separations. pH stable from 1 to 13. Robust (virtually any HPLC solvent can be used). Hydrophilic polymer structure delivers fasteroligonucleotide purification.21-mer Oligonucleotide Å, 54.1µmx 50 One particle size: 5 µm. Three column diameters: 2.1, 4.6 and 10 mm. Analytical and semiprep/preparative guard columns.024Minutes68104Mobile phase: A: 0.1 mol/TEAA,ph 7 B: 0.1 mol/LTEAA, ph 7, 25% AcetonitrileGradient: 30-80 %B in 15 min. Flow Rate: 0.60 mL/minColumn Temp: 60 oC Detection: UV @ 260 nmOrdering InformationHPLC ColumnsI.D.50 mm2.1 mm4.6 mm7926110 mm100 mm150 mm792707927179262792727925579266250 mmAnalytical Guard Columns79273Starter Kit79267(1 holder, 2 cartridges)Replacement Cartridges (5/pk)For Steel Columns*79268* Analytical guard column for steel columns is 2.3 x 20 mmVisit the HAMILTON Application Compound Index atwww.hamiltoncompany.com/hplc/app index 1.aspor e-mail chromatography@hamiltoncompany.comfor a complete list of compounds that have been separated.Semiprep/Preparative Guard ColumnFor Steel Columns***Starter Kits(1 holder, 1 cartridge)79277Replacement Cartridges (2/pk)79278*** Semiprep/preparative guard column for steel columns is 4.6 x 20 mm5

PRP-1 Reversed Phase HPLC ColumnsPolymeric 100 Å columns for general reversed phase separations. pH stable from 1 to 13. Better sample recovery than silica-based columns (there are no silanol groups). Excellent durability (stable to any concentration of water or organic solvent). Five particle sizes: 5, 7, 10, 12-20 and 30-50 µm. Eleven column internal diameters: 1.0 to 101.6 mm. Two column materials: 316 stainless steel and PEEK. Analytical and semiprep/preparative guard columns.Small Molecules1 2DNA with Secondary StructurePRP-1, 4.1 x 50 mm,3 µm (P/N 79804)211. Cytosine2. Uracil3. UridinePRP-1, 4.1 x 150 mm,10 µm (P/N 79425)Biocides found in SoapPRP-1, 4.1 x 250 mm,7 µm (P/N 79422)21. Failure Sequences2. 5’-DMT 36mer1. Chloroxylenol400 ppm2. Triclocarban100 ppm31003060Seconds90Conditions: 0.05M Citric Acid pH 4.2.Isocratic. Ambient. 1 mL/min. 10 µL, UV 254 nm.10Minutes20Conditions: A) 50mM Sodium HydroxidepH 12.7; B) 1:1 50mM Sodium Hydroxide : Acetonitrile.Linear Gradient 0% B (0-2 min), 0-50% B (2-25 min).60 C. 2 mL/min. UV 260nm.048Minutes1216Conditions: 4:1 Acetonitrile : Water. Isocratic. Ambient.1 mL/min. 10 µL, UV 254 nm.6

PRP-1 Reversed Phase HPLC ColumnsSurfactantSurfactantPRP-1, 4.1 x 150 mm,10 µm (P/N 79425)Fatty AcidsPRP-1, 4.1 x 150 mm, 10 µm (P/N 79425)1PRP-1, 4.1 x 100 mm, 3 µm (P/N 79805)11. Sodium Octadecylsulfate200 ppm1. Caproic Acid2. Caprylic Acid3. Capric Acid4. Lauric Acid5. Palmitic Acid6. Stearic Acid11. Sodium Dodecylsulfate200 ppm23002Minutes4Conditions: 4.5:5.5 Acetonitrile : Deionized Water.Isocratic. Ambient. 1 mL/min. 10 µL, Conductivity.Isomeric C18 Fatty Acids‑PRP-1, 4.1 x 250 mm, 7 µm (P/N 79422)024Minutes68Conditions: 3:7 Acetonitrile : Deionized Water. Isocratic.Ambient. 1 mL/min. 10 µL, ConductivityChlorhexidine GluconatePRP-1, 4.1 x 150 mm, 10 µm (P/N 79425)145 6510Minutes15Conditions: A) 3:1 Acetonitrile : Water; B) Acetonitrile.Linear Gradient 0-100% B in 2 min. Hold for 15 min.Ambient. 0.5 mL/min. 50 µL, UV 254 nm.ResveratrolPRP-1, 4.1 x 250 mm, 7 µm (P/N 79422)11. Linolenic Acid (C18:3)2. Linoleic Acid (C18:2)3. Oleic Acid (C18:1)4. Stearic Acid (C18:0)2 31. Resveratrol11. Chlorhexidine Gluconate2 µg4048Minutes1216Conditions: 0.1% Trifluoroacetic Acid in 9:1Acetonitrile : Water. Isocratic. Ambient. 0.8 mL/min.100 µL, UV 210 nm.Pyridinoline024MinutesConditions: 1:30:69 Trifluoroacetic Acid :Acetonitrile : Deionized Water. Isocratic. Ambient.2 mL/min. 1 µL, UV 254 nm.DiphenylguanidinePRP-1, 4.1 x 150 mm, 10 µm (P/N 79425)1122Minutes4Conditions: 1:9 Acetonitrile : Deionized Water with0.1% Heptafluorobutyric Acid. Isocratic. Ambient.2 mL/min. 3 µL, UV 280 nm.70369MinutesConditions: 3:7 Acetonitrile : 0.1M SodiumDihydrogen Phosphate pH 11.1. Isocratic. Ambient.1 mL/min. 10 µL, UV 280 nm.PRP-1, 4.1 x 250 mm,7 µm (P/N 79422)11. Diphenylguanidine1 mg/mL02Minutes12Folic AcidPRP-1, 4.1 x 150 mm, 10 µm (P/N 79425)1. Pyridinoline, 3 mg/mL064Conditions: 2:8 Acetonitrile : Deionized Water with0.1 % Trifluoroacetic Acid. Isocratic. Ambient.2 mL/min. 10 µL, UV 280 nm.1. Folic Acid0510Conditions: 97.5:2.5 20mM Sodium PhosphatepH 6.2 : Acetonitrile. Isocratic Ambient.1 mL/min. 5 µL, UV 280 nm.