Transcription
Thermo ScientificTechnical Resources Document1.51.00.5LC Columnsand Accessories
LC Column Selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-002HPLC Column Selection by U.S. Pharmacopeia Specifications . . . . . . . . . . . . . . . . . . . . . . . 4-007Thermo Scientific HPLC Phases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-009HPLC Column Selection by Manufacturer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-011Principles of Fast LC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-016LC Technical Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-018Derivatization Reagents for HPLC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-031www.thermoscientific.com/chromatographyLC Columns and AccessoriesSection Contents4-001
LC Columns and AccessoriesLC Column SelectionInformation in the following section will help you make an informed decision on theappropriate HPLC column for your application, based on stationary phase use, analyteproperties, LC-MS requirements or USP specifications. You will also find a useful tableof Thermo Scientific phases with specifications, as well as recommended ThermoScientific alternatives for other popular columns.Refer to the Advanced User Graphic (AUG) on thecorresponding product page (illustrated to the right)for more help and information on general purposecolumn selection.The AUG will show you Hydrophobicity which givesthe relative retention on the column. Generally, thehigher the hydrophobicity, the greater the retentionof neutral compounds and the higher the organiccontent in the mobile phase. A lower value indicatesa need for higher aqueous mobile phases to achievecomparable retention and resolution. Therecommended pH Range for the column is illustrated,outside of which column lifetimes will diminish.The Pore Size is shown, with larger pore size columnsbeing more applicable to larger analytes such asproteins or peptides. The percentage Carbon Loadis related to the hydrophobicity. Below the icon, youwill see the particle sizes available, as well as theUSP code. These graphics are designed to allow youto quickly compare the main characteristics of multiplestationary phases, allowing you to choose quickly themost appropriate stationary phase for your analysis.For additional help in column selection, pleasesee the back cover to contact our expert TechnicalSupport and tap into our expertise to help makethe best choice for your application.HydrophobicityLowHighpH Range02 to 814Pore Size080Å300Carbon Load (%)05Particle Size 2.6µm25USP L11Advanced User Graphic (AUG)Thermo Scientific Chromatography Columns and Consumables 2016-2017Common HPLC Phases and Their ilicaSilica-OHC1SAS-(CH3) 3 Least retentive of all alkyl group bonded phases for non-polar solutes.Typically used for moderately polar and multi-functional compounds.C4Butyl-C 4H9 Shorter retention than C8, C18. Separation of peptides and proteins.C8MOS-C 8H17 Less retentive than C18; normally used for small peptides andproteins, pharmaceuticals, steroids, environmental samples.C18ODS-C18H37 Most retentive of the alkyl-bonded phases. Used widely forpharmaceuticals, steroids, fatty acids, phthalates, environmental etc.CyanoCPS, CN-(CH2) 3CN Unique selectivity for polar compounds, more suitable than basesilica for normal phase gradient separations. When used in reversedphase, the selectivity is different to that of the C8 and C18 phases.Useful for a wide range of pharmaceutical applications and formixtures of very different solutes. AminoAPSPhenylPentafluorophenylPFPDiol-(CH2) 3NH2NormalPhaseReverse IonHILICPhaseExchange ApplicationNon-polar and moderately polar organic compounds.HILIC: Carbohydrate analysis and other polar compounds.Weak anion exchange: anions and organic acids.Normal Phase: Alternative selectivity to silica. Good for aromatics.-(CH3)C 6H5 Aromatic compounds and moderately polar compounds.-C 6F 5 Extra selectivity and retention for halogenated, polar compoundsand isomers.-(CH 2) 2OCH 2 (CH 2 OH) 2 SCXStrong CationExchanger-RSO 3H- SAXStrong AnionExchanger-RN (CH3) 3 AXAnion graphiticcarbonPGC100% carbon Reversed Phase: Proteins, peptides.Normal Phase: Similar selectivity to silica, but less polar.Organic bases.Organic acids, nucleotides and nucleosides.Organic acids, nucleotides and oligonucleotides.Particularly useful for the separation of highly polar compounds thatare difficult to retain using conventional silica based columns;separation of structurally similar compounds (e.g., isomers,diastereoisomers).
Before beginning a new analysis, consider the physical and chemical properties of the analyte(s), the mode of analysis and how theanalyte(s) will interact with the surface of the chromatographic phase. To aid column selection, the following guide may be useful.Non-polarAnalyteSolubiltyAnalyte Chemical PropertiesAcidicpKa 2Mode of SeparationRecommended PhaseAnion exchange / Reversed-phase mixed modeAcclaim Mixed-Mode WAX-1Acclaim Trinity P1Accucore C18Accucore RP-MSAccucore C8Accucore C30Accucore Phenyl-HexylAcclaim 120 C18Non-polarAcclaim 120 C8Reversed phaseAcclaim C30Acclaim Phenyl-1LC Columns and Accessories HPLC Stationary Phase Column SelectionHypersil GOLDHypersil GOLD C8Hypersil GOLD C4NeutralSyncronis C18Syncronis C8Accucore aQAccucore Polar PremiumAccucore PFPAccucore Phenyl-XAcclaim PolarAdvantagePolarsolventsModerately-polarAcclaim PolarAdvantage IIReversed phaseHypersil GOLD aQHypersil GOLD PFPHypersil GOLD PhenylSyncronis aQSyncronis PhenylAccucore C18Accucore RP-MSAccucore C8Accucore C30Accucore Phenyl-HexylAcclaim 120 C18Acclaim 120 C8Reversed phaseBasicAcclaim C30pKa 10Acclaim Phenyl-1Hypersil GOLDHypersil GOLD C8Hypersil GOLD C4Syncronis C18Syncronis C8Cation exchange / Reversed-phase mixed modeAcclaim Mixed-Mode WCX-1Acclaim Trinity P1www.thermoscientific.com/chromatography4-003
LC Columns and AccessoriesHPLC Stationary Phase Column Selection continuedPolarAnalyteSolubiltyAnalyte Chemical PropertiesMode of SeparationPolar retention effect on graphiteAnion exchangeAcidicRecommended PhaseHypercarbHypersil GOLD AXHypersil GOLD SAXAccucore Urea-HILICpKa 2HILICAccucore 150-Amide-HILICAcclaim HILIC-10Syncronis HILICPolar retention effect on graphiteHypercarbAccucore HILICAcclaim Mixed-Mode HILIC-1Acclaim Mixed-Mode WAX-1PolarsolventsAcclaim Mixed-Mode WCX-1NeutralHILICAcclaim Trinity P1Hypersil GOLD AminoHypersil GOLD HILICSyncronis AminoSyncronis HILICPolar retention effect on graphiteCation exchangeBasicHypercarbAcclaim Mixed-Mode WCX-1Acclaim Trinity P1Accucore Urea-HILICpKa 10HILICAccucore 150-Amide-HILICAcclaim HILIC-10Syncronis HILICHypersil GOLD AminoHypersil GOLD CNNon-polarsolventsNormal phaseHypersil GOLD SilicaSyncronis AminoSyncronis SilicaThermo Scientific Chromatography Columns and Consumables 2016-2017The above table recommends columns for the separation of small molecule analytes.4-004
The Thermo Scientific range offers a broad array of column designs and stationary phases optimized forLC-MS applications. Use the following table to help you choose your column format to best meet yourapplication needs. A variety of HPLC column hardware configurations are available, designed to give superiorresults for high speed, high sensitivity, high efficiency and convenience. A wide range of particle andmonolithic stationary phases allows choices for optimized selectivity.Column Hardware Selection for LC-MSLC-MS ApplicationHigh throughput analysisColumn Hardware DesignJavelin HTS columnsHigh sensitivity analysisAcclaim PepMap nano, capillaryand micro columnsEASY-Spray columnsProteomics analysisAcclaim PepMap nano columns,Accucore nano columns, nanoViperAcclaim PepMap nano columns,classicEASY-ColumnPepSwift monolithic columnsDescriptionDirect-connection columnsSlim design, 20mm length, 1mm to 4.6mm IDNano, capillary and micro columns 0.075mm to 1mm ID; 50 to 250mm lengthnanoViper format offers fingertight dead volume free connection to 1000 barCombined column/emitter design with nanoViper connection.Heated flexible silica columns 50µm and 75µm ID; 150 to 500mm lengthnanoViper offers fingertight dead volume free connection to 1000 barFlexible silica columns 50μm and 75μm ID; 50 to 500mm lengthTrap column 20mm x 100μm IDFlexible silica columns 50μm and 75μm ID; 50 to 500mm lengthTrap column 20mm x 100μm IDFlexible silica columns 100mm x 75μmTrap column 20mm x 100μm IDFlexible fused silica columns, nanoViper connections100µm to 500µm ID, 50 to 250mm lengthTrap columns 5mm x 200µm IDLC Columns and Accessories Column Format Selection for LC-MSVarious HPLC columns, throughout this LC section, can also be used for LC-MS application. Typical flow rates and MS sourcecompatibility for these columns are shown in the table on the next pagewww.thermoscientific.com/chromatography4-005
LC Columns and AccessoriesColumn Selection for LC-MS continuedPacked column selection for LC-MSAnalyteMolecularWeightSamplePolarityInterface RelativeIonization SensitivityLowColumn ID(mm)Flow Rate(µL/min)Low4.6, 4.0, 3.02000 – 200High2.1, 1.0200 – 50Low4.6, 4.0, 3.02000 – 200High2.1, 1.0200 – 50Low2.1, 1.0200 – 501.0 – 0.350 – 5Acclaim PepMap (RSLC) capillary and micro0.2 – 0.052 – 0.2EASY-Spray column, EASY-Column, Acclaim PepMap (RSLC) nano, Accucore nano2.1, 1.0200 – 501.0 – 0.350 – 5Acclaim PepMap (RSLC) capillary and micro0.2 – 0.052 – 0.2EASY-Spray column, EASY-Column, Acclaim PepMap (RSLC) nano, Accucore nano2.1, 1.0200 – 501.0 – 0.350 – 5Acclaim PepMap (RSLC) capillary and micro0.2 – 0.052 – 0.2EASY-Spray column, EASY-Column, Acclaim PepMap (RSLC) nano, Accucore nanoAPCIAPCI 1000 DaMediumESIHighHigh(or ionizable)LowESIHighLow 1000 DaColumn HardwareESIHighJavelin HTS, AnalyticalAnalytical, Javelin HTSJavelin HTS, AnalyticalAnalytical, Javelin HTSAnalytical, Javelin HTSAnalytical, Javelin HTSAnalytical, Javelin HTSMonolith columns for LC-MSAnalyte Molecular WeightColumn ID (mm)Flow Rate (µL/min) 1000 Da0.1, 0.2, 0.50.7 – 25PepSwift Monolith 1000 Da1.040 – 200ProSwift MonolithThermo Scientific Chromatography Columns and Consumables 2016-2017ProSwift is also available in larger IDs for high throughput applications.4-006Column Hardware
USP CodeL1DescriptionOctadecyl silane (C18) chemically bonded to porousor ceramic microparticles, 1.5 to 10μm in diameter,or a monolithic rodL3Porous silica particles, 1.5 to 10μm in diameter,or a monolithic rodL7Octyl silane chemically bonded to totallyor superficially porous silica particles,1.5 to 10μm in diameter, or a monolithic rodL8An essentially monomolecular layer ofaminopropylsilane chemically bonded to totallyporous silica gel support, 1.5 to 10μm in diameterNitrile groups chemically bonded to porous silicaparticles, 1.5 to 10μm in diameterPhenyl groups chemically bonded to porous silicaparticles, 1.5 to 10μm in diameterL10L11L13Trimethylsilane chemically bonded to porous silicaparticles, 3 to 10μm in diameterL14Silica gel having a chemically bonded, strongly basicquaternary ammonium anion exchange coating,5 to 10μm in diameterHexylsilane (C6) chemically bonded to totally poroussilica particles, 3 to 10μm in diameterL15Recommended PhaseAcclaim 120 C18Acclaim 300 C18Accucore C18Accucore aQAccucore 150-C18Accucore XL C18BioBasic 18Hypersil GOLDHypersil GOLD aQAcclaim PepMap 100 C18Syncronis C18Syncronis aQAccucore HILICHypersil GOLD SilicaSyncronis SilicaAcclaim 120 C8Accucore C8Accucore XL C8BioBasic 8Hypersil GOLD C8Acclaim PepMap 100 C8Syncronis C8Hypersil GOLD AminoSyncronis AminoLC Columns and Accessories HPLC Column Selection by U.S. Pharmacopeia Specifications*Hypersil GOLD CNAccucore Phenyl-HexylHypersil GOLD PhenylSyncronis PhenylBETASIL C1Hypersil SASHypersil GOLD SAXHypersil SAXBETASIL C6www.thermoscientific.com/chromatography4-007
LC Columns and AccessoriesHPLC Column Selection by U.S. Pharmacopeia Specifications* continuedUSP CodeL17L19L20L26L33L34L43L46L50L52Thermo Scientific Chromatography Columns and Consumables 2016-2017L584-008L59L60L62L78DescriptionStrong cation exchange resin consisting of sulfonatedcross-linked styrene-divinylbenzene copolymer in thehydrogen form, 6 to 12μm in diameterStrong cation exchange resin consisting of sulfonatedcross-linked styrene-divinylbenzene copolymer in thecalcium form, about 9μm in diameterDihydroxypropane groups chemically bonded toporous silica or hybrid particles, 1.5 to 10μmin diameterButyl silane chemically bonded to totally porous silicaparticles, 1.5 to 10μm in diameterRecommended PhaseHyperREZ XP Carbohydrate H HyperREZ XP Organic AcidsHyperREZ XP Carbohydrate Ca2 HyperREZ XP Sugar AlcoholsBETASIL DiolAccucore 150-C4BioBasic 4Hypersil GOLD C4Acclaim PepMap 300 C4Packing having the capacity to separate dextransBioBasic SEC 120by molecular size over a range of 4,000 to 500,000BioBasic SEC 300daltons. It is spherical, silica-based, and processedBioBasic SEC 1000to provide pH stabilityMAbPac SEC-1Strong cation exchange resin consisting of sulfonated HyperREZ XP Carbohydrate Pb2 cross-linked styrene-divinylbenzene copolymer in thelead form, 7 to 9μm in diameterPentafluorophenyl groups chemically bonded to silica Accucore PFPparticles by a propyl spacer, 1.5 to 10μm in diameter Hypersil GOLD PFPPolystyrene/divinylbenzene substrate agglomerated OmniPac PAX-100with quaternary amine functionalized latex beadsabout 9 to 11um in diameterMultifunction resin with reverse-phase retention and OmniPac PAX-500strong anion-exchange functionalities. The resinconsists of ethylvinylbenzene, 55% cross-linked withdivinylbenzene copolymer, 3 to 15µm in diameter, anda surface area of not less than 350m2 per g.Substrate is coated with quaternary ammoniumfunctionalized latex particles consisting of styrenecross-linked with divinylbenzeneA strong cation exchange resin made of porous silica BioBasic SCXwith sulfopropyl groups 5 to 10μm in diameterStrong cation exchange resin consisting of sulfonated HyperREZ XP Carbohydrate Na cross-linked styrene-divinylbenzene copolymer in thesodium form, about 6 to 30μm in diameterPacking for the size exclusion separation of proteins BioBasic SEC 300 (5μm)(separation by molecular weight) over the range ofMAbPac SEC-15 to 7,000 kDa. The packing is spherical, 1.5 to 10um,silica or hybrid packing with a hydrophilic coatingSpherical, porous silica gel, 10μm or less in diameter, Acclaim PolarAdvantagethe surface of which has been covalently modifiedAcclaim PolarAdvantage IIwith alkyl amide groups and endcappedAccucore Polar PremiumC30 silane bonded phase on a fully porous spherical Acclaim C30silica, 3 to 15µm in diameterAccucore C30A silane ligand that consists of both reversed-phase Acclaim Mixed-Mode WAX-1(an alkyl chain longer than C8) and anion-exchange(primary, secondary, tertiary or quaternary aminogroups) functional groups chemically bonded toporous or non-porous or ceramic micro-particles,1.0 to 50μm in diameter or a monolithic rod*These are the recommended Thermo Scientific HPLC columns for various USP categories although other columnsfor each category are also available.
The tables below list Thermo Scientific HPLC sorbents offered for small molecules separations.Please also refer to the Advanced User Graphic (AUG) for each HPLC phase on the pages indicated.PhaseParticle TypeAcclaim Phases120 C18Spherical, fully porous silica300 C18Spherical, fully porous silica120 C8Spherical, fully porous silicaPhenyl-1Spherical, fully porous silicaC30Spherical, fully porous silicaPASpherical, fully porous silicaPA IISpherical, fully porous silicaHILIC-10Spherical, fully porous silicaTrinity P1Nano polymer silica hybridTrinity P2Nano polymer silica hybridTrinity Q1Nano polymer silica hybridMixed Mode HILIC-1 Spherical, fully porous silicaMixed Mode WAX-1 Spherical, fully porous silicaMixed Mode WCX-1 Spherical, fully porous silicaOrganic AcidSpherical, fully porous silicaSurfactantSpherical, fully porous silicaSurfactant PlusSpherical, fully porous silicaExplosivesSpherical, fully porous silicaCarbamateSpherical, fully porous silicaCarbonyl C18Spherical, fully porous silicaSECSpherical, resinAccucore PhasesRP-MSSpherical, solid core silicaC18Spherical, solid core silicaC8Spherical, solid core silicaaQSpherical, solid core silicaPolar PremiumSpherical, solid core silicaPhenyl-HexylSpherical, solid core silicaPhenyl-XSpherical, solid core silicaPFPSpherical, solid core silicaC30Spherical, solid core silicaHILICSpherical, solid core silicaUrea-HILICSpherical, solid core silicaAccucore XL PhasesC18Spherical, solid core silicaC8Spherical, solid core silicaParticle Size(µm)PoreNominal Surface %EndcappingSize (Å) Area (m2/g)CarbonUSPCodePhaseCode2.2, 3, 532.2, 3, 533, 52.2, 3, 52.2, 3, 5333333, 53, 53, 53, 53, 53, 53, 52.25, 2www.thermoscientific.com/chromatographyLC Columns and Accessories Thermo Scientific HPLC Phases4-009
LC Columns and AccessoriesThermo Scientific Chromatography Columns and Consumables 2016-20174-010Thermo Scientific HPLC Phases continuedPhaseParticle TypeHypercarb PhaseHypercarbSpherical, porous graphitic carbonHypersil PhasesODS (C18)Spherical, fully porous silicaODS-2 (C18)Spherical, fully porous silicaMOS (C8)Spherical, fully porous silicaMOS-2 (C8)Spherical, fully porous silicaSAS (C1)Spherical, fully porous silicaPhenylSpherical, fully porous silicaPhenyl-2Spherical, fully porous silicaCPSSpherical, fully porous silicaCPS-2Spherical, fully porous silicaAPS-2Spherical, fully porous silicaSilicaSpherical, fully porous silicaSAXSpherical, fully porous silicaHypersil BDS PhasesC18Spherical, fully porous silicaC8Spherical, fully porous silicaPhenylSpherical, fully porous silicaCyanoSpherical, fully porous silicaHypersil GOLD PhasesC18 selectivitySpherical, fully porous silicaC8Spherical, fully porous silicaC4Spherical, fully porous silicaaQSpherical, fully porous silicaPFPSpherical, fully porous silicaPhenylSpherical, fully porous silicaCN (Cyano)Spherical, fully porous silicaAminoSpherical, fully porous silicaAXSpherical, fully porous silicaSAXSpherical, fully porous silicaSilicaSpherical, fully porous silicaHILICSpherical, fully porous silicaHypersil Green PhasePAHSpherical, fully porous silicaSyncronis PhasesC18Spherical, fully porous silicaC8Spherical, fully porous silicaaQSpherical, fully porous silicaPhenylSpherical, fully porous silicaAminoSpherical, fully porous silicaSilicaSpherical, fully porous silicaHILICSpherical, fully porous silicaParticle Size(µm)PoreNominal Surface %EndcappingSize (Å) Area (m2/g)CarbonUSPCodePhaseCode3, 5250120100––3503, 53, 53, 555553, 553, 53, , 3, 52.4, 3, 53, 53, 102812822892881.9, 3, 5, 121.9, 3, 51.9, 3, 51.9, 3, 51.9, 3, 51.9, 3, 51.9, 3, 51.9, 3, 51.9, 3, 51.9, 3, 51.9, 3, 51.9, 3, ��L14L3–2502522552532542592582572612632512653, 512017013.5Yes–3111.7, 3, 51.7, 3, 51.7, 3, 51.7, 3, 51.7, 3, 51.7, 3, 51.7, 3, 2973979977970975
To find a suitable Thermo Scientific alternativeto another manufacturer’s columns, refer to theselection guide below. The Thermo Scientificalternative phases are selected based on acombination of physical and chemicalsimilarities as well as mode of retention.These alternatives are not guaranteed toPhaseACE C18ACE C8ACE PhenylACE AQACE C18-300ACE C8-300ACE C4-300ACQUITY UPLC BEH HILICACQUITY UPLC HSS C18ACQUITY UPLC BEH C18ACQUITY UPLC BEH C8ACQUITY UPLC BEH PhenylACQUITY UPLC HSS T3Aeris PEPTIDE XB-C18Aeris WIDEPORE XB-C18Aeris WIDEPORE XB-C8Aeris WIDEPORE XB-C4Alltima HP C18Alltima HP C18 AQAlltima HP C18 HiLoadAlltima HP C8Alltima HP CNAlltima HP SilicaAminex HPX42CAminex HPX72SAminex HPX87CAminex HPX87HAminex HPX87NAminex HPX87PAQUA C18Ascentis C18Ascentis C8Ascentis Express C18Ascentis Express C8Ascentis Express F5Ascentis Express HILICAscentis ExpressPhenyl-HexylAscentis ExpressRP-AmideAscentis Express PeptideES-C18Ascentis Phenylprovide the same retention or selectivity, butshould be suitably similar in character to allowa similar or improved separation to be achievedwith some method optimization. The user shouldrefer to the individual phase information toensure that the characteristics of the alternativematch the requirements of their separation.The following table is not complete in termsof manufacturer or products offered. Althoughevery effort is made to ensure that the productinformation provided is as accurate as possible,some errors may occur in collation and transcription.We cannot accept any responsibility for the useof the following coPore Size 9090909090Area 50150150150150% mmended Thermo Scientific AlternativeSyncronis C18Syncronis C8Syncronis PhenylSyncronis aQBioBasic 18BioBasic 8BioBasic 4Hypersil GOLD Silica (1.9µm)Hypersil GOLD (1.9µm)Hypersil GOLD (1.9µm)Hypersil GOLD C8 (1.9µm)Hypersil GOLD Phenyl (1.9µm)Hypersil GOLD aQ (1.9µm)Accucore 150-C18Accucore 150-C18Accuccore 150-C4Accuccore 150-C4Hypersil GOLDHypersil GOLD aQSyncronis C18Hypersil GOLD C8Hypersil GOLD CNHypersil GOLD SilicaHyperREZ XP Carbohydrate Ca2 HyperREZ XP Carbohydrate H HyperREZ XP Carbohydrate Ca2 HyperREZ XP Carbohydrate H HyperREZ XP Carbohydrate Na HyperREZ XP Carbohydrate Pb2 Hypersil GOLD aQSyncronis C18Syncronis C8Accucore C18Accucore C8Accucore PFPAccucore HILICAccucore Phenyl-HexylSupelco90150–Accucore Polar PremiumSupelco16080–Accucore 150-C18Supelco10045019Syncronis Phenylwww.thermoscientific.com/chromatographyLC Columns and Accessories HPLC Column Selection by Manufacturer4-011
LC Columns and AccessoriesThermo Scientific Chromatography Columns and Consumables 2016-20174-012HPLC Column Selection by Manufacturer continuedPhaseAtlantis dC18Atlantis T3Atlantis HILIC SilicaAtlantis dC18Capcell Core C18Capcell Pak C18 AQCortecs C18Cortecs C18 Cortecs HILICDiscovery BIO Wide Pore C18Discovery BIO Wide Pore C8Discovery C18Discovery C8Discovery CyanoGemini C18Halo C18Halo C8Halo HILICHalo PFPHalo Phenyl-HexylHalo RP-AmideHALO Peptide ES-C18Inertsil C4Inertsil C8Inertsil ODS3VInertsil PhenylInertsil SilicaJ’Sphere M80Jupiter C18Jupiter C4Kinetex C18Kinetex C8Kinetex HILICKinetex PFPKinetex Phenyl-HexylKinetex XB-C18Kromasil C18Kromasil C4Kromasil SilicaLiChrospher CNLiChrospher DiolLiChrospher NH2LiChrospher RP 18LiChrospher RP-18eLiChrospher RP-8LiChrospher RP-8eLuna C18 (2)Luna C8 (2)Luna CNLuna HILICLuna NH2Luna PFP oSupelcoPhenomenexAMTAMTAMTAMTAMTAMTAMTGL SciencesGL SciencesGL SciencesGL SciencesGL omenexPhenomenexPore Size rea 00200400400% ��5212213131814–5.7105.7Recommended Thermo Scientific AlternativeAcclaim Polar Advantage IIHypersil GOLDHypersil GOLD SilicaHypersil GOLD aQAccucore C18Acclaim Polar Advantage IIAccucore C18Accucore Polar PremiumAccucore HILICBioBasic 18BioBasic 8Hypersil GOLDHypersil GOLD C8Hypersil GOLD CNHypersil GOLDAccucore C18Accucore C8Accucore HILICAccucore PFPAccucore Phenyl-HexylAccucore Polar PremiumAccucore 150-C18Hypersil GOLD C4Syncronis C8Syncronis C18Syncronis PhenylSyncronis SilicaAcclaim PolarAdvantage IIBioBasic 18BioBasic C4Accucore C18Accucore C8Accucore HILICAccucore PFPAccucore Phenyl-HexylAccucore C18Syncronis C18Hypersil GOLD C4Syncronis SilicaHypersil GOLD CNBETASIL DiolSyncronis AminoSyncronis C18Syncronis C18Syncronis C8Syncronis C8Syncronis C18Syncronis C8Hypersil GOLD CNBETASIL DiolSyncronis AminoHypersil GOLD PFP
lentAgilentAgilentPore Size 20120Area –120120120130% �202857.5–Recommended Thermo Scientific AlternativeBioBasic SCXSyncronis SilicaHypersil GOLDHypersil GOLD CNHypersil APS-2Hypersil GOLD PhenylHypersil GOLDHypersil GOLD C8Hypersil GOLD CNHypersil GOLD PhenylHypersil GOLD SilicaSyncronis C18Syncronis C18Hypersil GOLD CNSyncronis aQAccucore C18Accucore Phenyl-HexylAccucore PFPSyncronis C18Syncronis C18Syncronis PhenylSyncronis C8Hypersil GOLD CNHypersil SAXSyncronis AminoBETASIL DiolBioBasic SCXHypersil GOLD SAXBioBasic 18BioBasic 4BioBasic PhenylBioBasic 8BioBasic CNHypersil SASHypersil GOLDHypersil GOLD C4Hypersil GOLD CNHypersil GOLDHypersil GOLD (1.9µm)Hypersil GOLD C8Hypersil GOLD CNHypersil GOLD PhenylHypersil GOLDHypersil GOLD AminoHypersil GOLD PhenylHypersil GOLD SAXHypersil GOLD SilicaSyncronis C18BioBasic 4Accucore C18Accucore C8Accucore C18Accucore aQwww.thermoscientific.com/chromatographyLC Columns and Accessories PhaseLuna SCXLuna Silica (2)µBondapak C18µBondapak CNµBondapak NH2µBondapak PhenylNova-Pak (HR) C18Nova-Pak C8Nova-Pak CNNova-Pak PhenylNova-Pak SilicaNUCLEODUR C18 ECNUCLEODUR C18 GravityNUCLEODUR CNNUCLEODUR PyramidNucleoshell RP 18Nucleoshell Phenyl-HexylNucleoshell PFPNucleosil 100 C18Nucleosil 100 C18 ABNucleosil 100 C 6 H 5Nucleosil 100 C8Nucleosil 100 CNNucleosil 100 N(CH 3) 2Nucleosil 100 NH2Nucleosil 100 OHNucleosil 100 SANucleosil 100 SBNucleosil 300 C18Nucleosil 300 C4Nucleosil 300 C 6 H 5Nucleosil 300 C8Nucleosil 300 CNPinnacle C1Pinnacle C18Pinnacle C4Pinnacle CNPinnacle DB C18Pinnacle DB C18 1.9µmPinnacle DB C8Pinnacle DB CyanoPinnacle DB PhenylPinnacle IBDPinnacle NH 2Pinnacle PhenylPinnacle SAXPinnacle SilicaPinnacle Ultra C18Pinnacle Wide Pore C4Poroshell 120 EC-C18Poroshell 120 EC-C8Poroshell 120 SB-C18Poroshell 120 SB-Aq4-013
LC Columns and AccessoriesThermo Scientific Chromatography Columns and Consumables 2016-20174-014HPLC Column Selection by Manufacturer continuedPhasePoroshell 120 Phenyl-HexylPoroshell 120 Bonus-RPPoroshell SB-C18Poroshell Extend-C18Poroshell 300 SB-C8Poroshell 300 SB-C3Primesep Prodigy C8Prodigy ODS2Prodigy ODS-3Prodigy ODS-3VProdigy Phenyl-3Purospher RP-18Purospher STAR-8ePurospher STAR RP-18eWaters Spherisorb C1Waters Spherisorb C6Waters Spherisorb C8Waters Spherisorb CNWaters Spherisorb NH2Waters Spherisorb ODS1Waters Spherisorb ODS2Waters Spherisorb ODSBWaters Spherisorb PhenylWaters Spherisorb SAXWaters Spherisorb SCXWaters Spherisorb W (silica)SunFire C18SunFire C8SunShell C18SunShell C8SunShell PFPSupelcosil LC-1Supelcosil LC-18Supelcosil LC-18DBSupelcosil LC-8Supelcosil LC-CNSupelcosil LC-NH 2Supelcosil LC-SiSymmetry C18Symmetry C8Synergi Hydro-RPTSKgel G2000SW (incl XL)TSKgel Octyl-80TSTSKgel ODS-120ATSKgel ODS-120TTSKgel ODS-80TMTSKgel Super OctylTSKgel Super ODSTSKgel Super PhenylTSKgel lcoSupelc
HPLC Stationary Phase Column Selection Before beginning a new analysis, consider the physical and chemical properties of the analyte(s), the mode of analysis and how the analyte(s) will interact with the surface of the chromatographic phase . To aid column selection, the following guide may be useful . Non-polar
