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AB SCIEX QTRAP 5500 SYSTEMToday’s mostsensitive andselective QTRAPAB SCIEX QTRAP 5500 SYSTEM

Powerful synergy results when the world’s mostsensitive triple quadrupole unites with innovative LinearAccelerator trap technology, the world’s most sensitiveand fastest scanning linear ion trap. The whole reallyis more than the sum of its parts. The unique hybridarchitecture, unparalleled sensitivity, and impressive speedenable solution-based workflows that solve problemsother mass spectrometry systems cannot. The QTRAP 5500 system is truly above and beyond the extraordinary,with no sacrifice in qualitative or quantitativeperformance, whether targeting specific compounds orcompound classes to generate accurate quantitation,or performing sensitive compound identification andconfirmation in a single run. Get more performance,better results, better efficiency, and be more productive.

Sensitivity meets selectivity for enhancedquantitative and qualitative performanceThe AB SCIEX QTRAP 5500 System is next-generation technology from the leaders in massspectrometry. Redesigned from the ground up, but bred from our industry standard triple quadrupoleline of mass spectrometers, the QTRAP 5500 System brings in a new era of performance.Fast, precise quantitative performanceUltra-fast triple quadrupole scan speeds improve precursor ion andneutral loss scan performance, ideal for fast chromatography. FastLC also demands incredibly short dwell times for MRM experiments.Let the innovative Scheduled MRM algorithm automaticallyNow monitor even more MRM transitions than ever thoughtpossible and still get the quantitative performance you have cometo expect from AB SCIEX—day after day, year after year.Driven by all-new Linear Accelerator trap technologyoptimize your dwell times and maximize your capacity to deliverIt doesn’t stop there. The QTRAP 5500 System also houses thegold-standard quantitation.most sensitive ion trap in the world—the all new, patented LinearAccelerator trap. With ultra-fast scan speeds, up to 100-foldincrease in trap scan MS/MS sensitivity, and full MS3 capabilities,there is no compromise in the qualitative data that are generated—or the confidence you will have in those data.

In a class by itselfMany mass spectrometry systems offer either quantitative or qualitative structural analysis. Butthe unique hybrid architecture of the QTRAP system means that you can get both exceptionalqualitative and quantitative information from the same instrument and in the same analytical run.The unparalleled scan speeds and sensitivity of the QTRAP 5500For example, predictive MRM (pMRM) identifies and characterizesSystem are made possible by advanced eQ electronics. Next-a comprehensive set of metabolites including extremely low levelgeneration eQ electronics in turn drive the unique TripleTrap metabolites in one time-saving analysis; screening for Pesticidesscanning technology to move from triple quadrupole mode toand Pharmaceutical and Personal Care Products (PPCP) is achievedLinear Accelerator trap mode in the blink of an eye, enablingwith ultimate confidence; and MRM assay development of peptidesworkflow-driven data acquisition that cannot be done on any otherand proteins using the MIDAS workflow results in more robustmass spectrometer. Intelligent linking of the system’s capabilitiesMRM assays and saves money and time by removing the need forwith TripleTrap scanning opens up powerful and uniquesynthetic or purified peptide or protein standards. workflows that accelerate your research and bring new levels ofproductivity and reproducibility.Matrix InterferenceMRM331/99MS 3331/99/71S/N8S/N147Accuracy130%Accuracy105%% CV32%% CV1.9%MS3 quantification provides better selectivity to improve the detection limits and alsoUltra-fast and selective MRM scans mean you can target and quantify hundreds toimprove the quality of the quantification data of analytes. Above is a comparisonthousands of compounds in a single analysis without compromise in data quality.of MRM versus MS3 selectivity for detecting 10 ppb Malathion in a 50x dilutedQuEchERS extract of apple. The transition 331/99 in the XIC on the left had anelevated background level and also matrix interference; in contrast, the MS3 transition,331/99/71 in the XIC on the right, showed superior selectivity, a better signal-to-noiseratio, improved accuracy and tighter %CV.

The power of TripleTrap scanning is illustrated in the above metabolite ID example. In this UHPLC experiment, two survey scans for characteristic GSH fragments, apositive ion neutral loss of m/z 129 and a negative ion precursor ion scan of m/z 272, automatically trigger enhanced product ion scans to screen for potential reactivemetabolites. Including the polarity switch between survey scans, the total cycle time is 2.1 seconds.M7 (3.50)1.1e41.0e4S/N 109.0e38.0e3Intensity, .03.54.03e74.54.092.5e7Intensity, cpsTime, min TIC7.597.502.0e7Intensity, cps4.921e793.0 138.387.0912345246.5220240m/z, Da3.75678910373.92e70e04.253.175.0e6NCH34.80 5.711.0e70.0e0OHNN91.1112.31.5e7N11Time, minHighest sensitivity Linear Accelerator trap technology can track MRM scans to the limits of detection.320.0260280300320340360380

Cutting edge technology and reliable tooDue to our long legacy as the industry leader, our customers expect maximum uptime and a systemthat can handle even the most difficult matrices with ease. Our Turbo V source and Curtain Gas interface are the benchmark for reliability. The new QJet ion guide, eQ electronics, Qurved LINAC collision cell, and AcQuRate pulse counting detector found in the QTRAP 5500 System have beendesigned to improve on our already industry-leading robustness. With reliability designed into thesystem, new levels of dependability and consistency are certain.The very low % CVof 3.3 for the analytepeak area of 1300injections of spikedAnalyte Peak AreaDiazepam in proteinprecipitated humanplasma demonstratesthe industry-leadingruggedness,reproducibility andreliability of theQTRAP 5500 System.Sample Number

Turbo V source provides high-QJet ion guide’s patented design yieldsNanoSpray III ion source is an easysensitivity analysis over a wide range ofimproved ion containment and operates atto assemble and disassemble nanoflowflow rates with quick-change APCI andhigh pressure, providing better collisionalsource that makes working with nanoflowTurboIonSpray probes. From 50 µL/minfocusing to enhance ion transmissionchromatography easy while providingto 5 mL/min, the Turbo V source is thefor ultimate sensitivity. The new designthe highest sensitivity and stability.perfect match for narrow bore, standardalso reduces the gas load, allowing theThe NanoSpray III source supports regularbore and UHPLC flow rates, deliveringturbopump to run cooler in its idealand column-packed emitter tips forunprecedented desolvation and stability foroperating range. All this leads to our mostultimate chromatographic flexibility, andeven the toughest high-flow applicationsreliable system yet and with tool-freethe new camera design allows clear sprayutilized to drive productivity.maintenance, clean-up is simplevisualization that simplifies optimization.and straightforward.Fingertight fittings enable you to changetips quickly so you are up and runningin no time.The excellent % CV of2.3 for the area ratio ofDiazepam and deuteratedinternal standard inprotein-precipitatedhuman plasma showsArea Ratiothe reproducibilityand stability of theAcQuRate pulsecounting detector forconsistent quantitificationresults day in day out,week after week, monthafter month.Sample Number

The future path of QTRAP technologyThe AB SCIEX QTRAP 5500 System brings together the latest hardware from the world’s bestselling triple quadrupole family, and adds next-generation, ultra-fast and sensitive LinearAccelerator trap functionality. Delivering unmatched qualitative and quantitative analysis—theQTRAP 5500 System enables productive, time-saving workflows that simply cannot be done withother mass spectrometry systems.AcQuRate Pulse Counting DetectorThe AcQuRate pulse counting detector is the latest in detector technologycombined with a pulse overlap correction algorithm, enabling more accurateand precise ion detection over a wide dynamic range. Operating at maximumgain all the time drives the detector into the digital domain, simplifying theelimination of electronic noise and guaranteeing maximum sensitivity withunparalleled accuracy and precision. The best gets even better.Patented QJet Ion GuideOptimized design yields better ion containment and operates at highpressure, providing better collisional focusing to enhance ion transmissionfor ultimate sensitivity. The new design also lets the turbopump run coolerand in its ideal operating range.Patented Q0 High-Pressure CellQ0 collisional focusing. Patented high pressure collisional focusingtechnology maximizes transmission of ions for superior sensitivity.Q0 trapping. Ions can be accumulated in the Q0 region while the LinearAccelerator trap is performing MS/MS and MS3 scans. This yields superiorsensitivity in ion trap mode, which can be extremely important for fastUHPLC applications where time and duty cycle are condensed.

Patented Linear Accelerator TrapBringing LINAC technology to the Q3 linear ion trap greatly improves theextraction efficiency to yield up to a 100 x gain in sensitivity in ion trap scanmodes. Now take full advantage of the 20,000 Da/s scan speeds withoutworrying if enough sensitivity is on board to generate incomparable results.Improved excitation efficiencies and reduced ion cooling and fragmentationtimes produce superior MS3 qualitative results and provide unprecedentedselectivity for the most challenging analytical assays.Patented Qurved LINAC Collision CellThe newly designed Qurved LINAC high-pressure collision cell acceleratesions through the collision cell, increases speed of analysis and eliminatescross-talk. Improving on the performance of the legendary LINAC collisioncell results in shorter transit times across the collision cell, making theQurved LINAC cell an ideal match for UHPLC and high throughputanalysis focused on hundreds of compounds. With true collision-inducedfragmentation, the new Qurved LINAC collision cell generates reliable,information-rich, library-searchable MS/MS spectra time after time.Fast eQ Electronics for Fast LCNext-generation eQ electronics means polarity switching in 50 msand scan speeds of 20,000 Da/s. Now, compounds with vastly differentfunctional groups can be measured in a single pass. The new electronicsalso provide improved ion containment for better sensitivity and superiordetector performance. Ultra-fast and ultra-stable instrumentation meansyou get the most out of your standard or fast LC to save time andaccelerate your research.