WIXLIB

SH800SCell SorterSony Biotechnology Inc.

SH800S Cell SorterSorting Made Simple The benchtop SH800S cell sorter permits sorting of a widerange of cell sizes for many applications using the 70-µm,100-µm, and 130-µm microfluidic sorting chips. This novel,chip-based design is fully integrated with comprehensive fluidiccontrols and advanced automation for setup, acquisition, sorting,and analysis to make sorting less subjective, more precise, andeasier to perform.System software is intuitive and supports sorting into tubesand 96- and 384-well plates. The software generates FCS 3.0and FCS 3.1 files that can be exported to third partyanalysis tools.The SH800S is flexible and can support a variety of applicationsfor individual and core labs. For applications in which cross-contamination is a concern, optional e-beam sterilized consumablescan be used to replace the sample line and sorting chip.An optional class II type A2 biosafety cabinet providesprotection for personnel and products. The biosafety cabinetwas custom designed for the SH800S and tested by The BakerCompany to meet many international standards.The optical design offers up to four collinear excitation lasers(488 nm, 405 nm, 561 nm and 638 nm) and six fluorescencedetectors. The six free-form PMTs enable detection of fluorescence signals from any laser, based on filter selection.The SH800S features a compact footprint(55 cm W x 55 cm D x 72 cm H) andbuilt-in automation to provide ease ofoperation. Provides the highest level of automation available in any cellsorter, allowing researcher setup, alignment, calibration, andmonitoring with a push of a button. A novel microfluidic sorting chip is available in three sizes including70-µm, 100-µm, and 130-µm to permit sorting of a wide range of cellsizes, which simplifies sorting. An optional custom biosafety cabinet that meets industry standardsfor personnel and product protection.

Automation from Setup to AnalysisThe SH800S provides the highest level of automationand ease of use available in a cell sorter. Researchers canaccurately set up, calibrate, and monitor sorting with a pushof a button. This delivers true ease of operation to save timeand improve consistency of results.A novel microfluidic sorting chip uses patented CoreFinder technology to automate key steps of instrument setupand operation, streamlining workflow.The system is versatile, allowing researchers to sort of a widerange of cell sizes to accommodate application requirements.Chip sizes include 70 µm, 100 µm, and 130 µm.7-inch LCD MonitorDisplay Mode ButtonChip Loader Flap DoorPower/Standby ButtonSample Collection Area DoorSample Collection Tube Load/Unload ButtonSample Loader DoorSample Collection Slide Door4

Automated Chip Loading and PositioningAutomated Optical Axis AdjustmentAutomated Droplet CalibrationSystem setup begins with a one-touchinstallation to load the microfluidic flow cellchip. Actuators ensure precise positioningof the chip inside the chip loader. Sheath,sample, and vacuum lines connect and sealautomatically to their respective ports oncethe chip is loaded. Made of durable plastic,the chip is easy to replace when needed.The alignment of the chip to the lasers isoptimized automatically during setup usingthe Sony patented Blu-ray technology foraligning and tracking laser position. On adaily basis, using AutoSetup beads, the Xand Z positions of the chip are adjusted toensure consistent results.The droplets are automatically calibrated byadjusting the frequency and the drop driveto achieve an optimal breakoff point (BOP)for each type of sorting chip.Automated Side Stream CalibrationAutomated Drop Delay CalibrationAutomated Sort MonitoringThe angle and the position of the sidestreams are calculated and adjusted during setup for tube and plate sorting. Thisensures that the sort stream is centered inthe collection tube automatically withoutmanual adjustment.A dedicated laser and camera performreal-time analysis of droplet images usingAutoSetup beads. Patented technologycalculates drop delay by capturing the fluorescence image of the drop-delay beads.The relationship between bead positionsand drop delay is used to calculate theoptimal drop delay to enable precise targeting and high yield. Automatic analysis of thebinary image of the droplets, in real time,calculates the drop delay using a patentedalgorithm.The SH800S software monitors and activelymakes adjustments to the drop drive tomaintain a stable breakoff point. This featureensures consistently good sort performanceand allows detection of clogs, and emptytubes, and facilitates walk-away operation.5

SoftwareSoftware, sensors, and CoreFinder patented technologyprovide automation across the workflow from setup toshutdown to ensure consistency, save time, and improve theaccuracy of results. The interface is designed with ribbon tabsthat logically organize features to make them rapidly accessible. An experiment-centric approach makes the softwareeasy to teach, learn, and use.Software wizards include step-wise workflows that guideusers through startup, multicolor compensation, sorting, andsystem cleaning. Since the system simplifies even advancedsorting techniques, it can be used by researchers with littlesorting experience.System StartupInitial Instrument Setup, Precision Alignment, and CalibrationsUpon startup, the system initializationincludes diagnostics that ensure all subsystems are properly working. Once verified,the system status and a green ready message are displayed on the LCD monitor onthe front of the instrument.The setup wizard guides researchersthrough the process of loading a sortingchip, selecting lasers, and inserting theoptical filters required.The LCD monitor on the front of the SH800Sdisplays status information during operation.Fluidics CheckWhen complete, the fluidics check starts,and sheath fluid droplets appear from thetip of the sample probe. If needed, debubble and sample line cleaning wizardscan be run. Once complete, the autocalibration screen is displayed.Alignment and CalibrationsThe system automatically and preciselyaligns the sorting chip to the lasers, calibrates the droplets and side streams,and estimates the drop delay usingAutoSetup beads.The QR code on the packaging is scannedto identify the chip information, includingnozzle size.The sorting chip is easy to load.Automatic calibration performs precisemeasurements using the side streammonitor. The software displays progress andstatus at each stage of calibration.To further simplify setup, the FluorochromeDetection Matrix screen displays where toinsert optical filters.6

Experiment SettingsData Analysis and DisplaySortingUsers can create an experiment by selectinga new template, a recent experiment, or ashared template (public) from the Experiment window. If a new template is selected,dialogs guide the user to choose experimentsettings such as sample groups, tubes, andpulse parameters for data acquisition. Oncea template is selected, the user can startacquisition or the compensation wizard.Data is displayed as dot plots and histograms on worksheets, and events can bemarked using gates. The software has anumber of tools to select, adjust, label, andmeasure statistics of target populations.Data can be easily exported in FCS formats(3.0 or 3.1) to use with third party analysissoftware.Once events are gated on plots, targetedcells can be sorted for further analysis.Sort control is managed in a simple dialogwhere settings and configurations areselected. The SH800S supports a varietyof sorting methods and vessels including2-way tubes, 8-well strips, slides, and6-, 12-, 24-, 48-, 96-, and 384-well plates.The system supports eight sorting modesto provide different levels of purity (including single-cell mode) and yield to enableoptimal results. In addition, the softwareprovides an assistive mode for large cells toimprove sorting performance. Three sorting chip nozzle sizes (70 µm, 100 µm, and130 µm) are available to accommodate arange of cell sizes and applications.Selecting a template or a recent experimenton the left of the window displays the experiment structure on the right.The Experiments tab provides easy accessto all lists of previous experiments as well asvisualization and statistics tools for analysis.Sorting settings and sort status are easilyaccessed and customized.System MaintenanceThe SH800S software uses wizards toguide users to perform routine tasks suchas fluidics cleaning for aseptic sorting andparts maintenance.7

OptionsBiosafety CabinetThe BCC300AMS Class II biosafety cabinetcustom designed for the SH800S by TheBaker Company is available as an option toprovide personnel and product protection.The cabinet measures 118 cm (W) x 99.1 cm(D) x 224 cm (H).Biosafety Standards ComplianceThe cabinet was tested by The BakerCompany using microbiological assayswith the SH800S sorter inside the workarea. The testing concluded that thebiosafety cabinet with the SH800S insidemet several international biosafetystandards including the National SanitationFoundation Standard 49 (NSF49) and theEuropean Standard 12469.Built-in Aerosol ManagementThe cabinet incorporates a built-in aerosolmanagement system which operatesindependently to actively evacuate aerosolsfrom the sort collection chamber. Thedual routes of aerosol evacuation ensuremaximum personnel protection.8

E-Beam Sterilized ConsumablesExchangeable Sample FluidicsVersatile Application SupportFor applications in which cross contamination between samples or external biologicalentities are a factor, optional e-beamsterilized consumables including chips,setup beads, and sample lines can be usedto streamline workflow.The SH800S sorting chip is an integratedflow cell/nozzle assembly. It containsmicrofluidic channels for controlling theflow of sample and sheath fluid. Withinthe chip, the sample is interrogated by thelasers before it passes through the nozzlefor sorting.The SH800S offers 70-µm, 100-µm, and130-µm microfluidic sorting chips to permitthe sorting of a wide range of cell sizes andapplications.Easy InstallationChip installation and removal are quickand easy to reduce the downtime associated with changing nozzles during setupand removal of clogs. The chip and thePEEK sample line-chip connector assembly,which come in contact with the sample, arefully disposable. This gives researchers theoption to change out the sample fluidicspath if needed.Sort Deposition SystemIndex Sorting SoftwareThe optional Sort Deposition Systemfacilitates high-throughput sorting andprecise deposition of cells into 6-, 12-, 24-,48-, 96-, and 384-well plates or PCR plates.Index sorting software records the X andY coordinates of each event sorted intoa multiwell device. This feature allowsresearchers to track the scatter andfluorescence intensity of individual cellssorted in each well.B80096-well plate holder600BSC-ASingle cells sorted into a 96-well plate.EGFP : 100.00%400200010 210 310 410 5CRISPR: EGFP-A10 69