PCR Solutions Brochure - Thermo Fisher Scientific

Transcription

PCR solutionsAmplification made for breakthroughs1

PCR solutions made for what you aspire to achieveIn your pursuit to advance science, every experiment matters.There’s no time to start again, no need to wonder if theproducts you chose will set you back or propel you forward.With our comprehensive portfolio of thermal cyclers, PCRplastics, reagents, and service plans, you know exactly whatyou’re getting—quality made to support your every win, bigand small, at every step.Learn more at thermofisher.com/pcrworkflow

ContentsWhich instrument fits your needs?4ProFlex PCR System6VeritiPro Thermal Cycler8SimpliAmp Thermal Cycler10MiniAmp Thermal Cycler12Automated Thermal Cycler14Thermal Cycler Fleet Control Software16Thermal cycler services and support16Engineer Approved MicroAmp PCR plastics18SuperScript IV Reverse Transcriptase22PCR enzymes25Nucleic acid separation and analysis27PCR primers and dNTPs31Specialty enzymes32PCR and molecular biology education323

Which instrument fits your needs?Providing precise, consistent results, Applied Biosystems thermal cyclers aredesigned for every challenge, application, and budget. Be confident in your results withinstruments designed for reliability, accuracy, and intuitive programming. Reduce timespent on PCR optimizations with Applied Biosystems VeriFlex Block temperaturecontrol technology. Manage your instruments remotely with secure access.Ultimate flexibility andthroughputUltimate performanceProFlexPCR SystemVeritiProThermal CyclerMax. sample throughput480,000 reactions384 reactionsMax. block ramp rate6.0 C/sec6.0 C/secBlock formats(temperature optimization) 3 x 32-well, 0.2 mL(2-zone VeriFlex Block) 96-well, 0.2 mL(6-zone VeriFlex Block) 96-well, 0.2 mL(6-zone VeriFlex Block) 384-well, 0.02 mL 2 x 96-well,0.2 mL 2 x flat block 2 x 384-well,0.02 mLFor Research Use Only. Not for use in diagnostic procedures. cloud-enabled instrument automation-ready instrument4

Elegantly simple and preciseRoutine PCRDesigned for easy roboticintegrationSimpliAmp Thermal CyclerMiniAmpThermal CyclerAutomated Thermal Cycler96 reactions96 reactions384 reactions4.0 C/sec3.0 C/sec3.5 C/sec 96-well, 0.2 mL(3-zone VeriFlex Block) 96-well, 0.2 mL 96-well, 0.2 mL, compatiblewith full- or semi-skirted plates 384-well, 0.02 mLLooking for a thermal cycler for in vitro diagnostic use? Learn more about theApplied Biosystems Veriti Dx Thermal Cycler at thermofisher.com/veritidxDon’t forget reagents—choose from enzymes such as Invitrogen SuperScript IV reversetranscriptases and Invitrogen Platinum SuperFi II reagents (see pages 22–26).Interested in private-label thermal cyclers or PCR plastics?To find out more, go to thermofisher.com/oem-partner5

Ultimate flexibility and throughputProFlex PCR SystemThe Applied Biosystems ProFlex PCR System combinesflexible configuration and control features to fit howyou work today and tomorrow with the reliability you’vecome to expect from Applied Biosystems products.Interchangeable block formats allow you to maximize yourthroughput or run independent experiments concurrently.The ProFlex PCR System is cloud-enabled, givingyou the freedom to design and securely upload yourmethods, monitor runs, and check instrument availabilityfrom any mobile device or desktop computer with theThermo Fisher Connect Platform. Multi-user accessible—run three experiments at once cloud-enabled instrument Flexible block configuration—accepts five differentblock formats for optimization and throughput Cloud-enabled—conveniently access your instrumentanytime, anywhere with the Connect Platform Fleet control–compatible—manage multipleinstruments, users, and methods withApplied Biosystems Thermal Cycler FleetControl SoftwareFive interchangeable block optionsThe ProFlex PCR System has five different blocks that canbe changed with the flip of a switch, including a 3 x 32-wellblock. This allows up to three experiments to be runsimultaneously, completely independently of each other.3 x 32-well96-wellDual 96-well and dual 384-well blocks are available forhigh-throughput needs. A dual flat block is also available tosupport Applied Biosystems OpenArray plate technologyfor genotyping analysis on the Applied Biosystems QuantStudio 12K Flex Real-Time PCR System as well asour sealed-chip technology on the QuantStudio 3D DigitalPCR System.Dual 96-wellDual 384-wellDual flatFind out more at thermofisher.com/proflex6

SpecificationsBlock formatFeaturesMax. block ramp rateMax. sample ramp rateTemperature accuracyTemperature rangeTemperature uniformityDimensions (H x W x D)WeightPCR volume rangeInstrument memoryDisplay interfacePowerVeriFlex BlockData connectivity †3 x 32-well, 0.2 mL,2-zone VeriFlex Blockindependent controlRun three experimentsat once or atdifferent times96-well, 0.2 mL,6-zone VeriFlexBlockPerform completeoptimization workwith full 96-wellVeriFlex Block6.0 C/sec4.4 C/sec2 x 96-well,0.2 mLHigh throughput in96-well format2 x flat block forchips and arraysHigh throughputcapability:8 x 3,072 OpenArray Plate*or 24 x 20K Chip**3.0 C/sec1.6 C/sec1.6 C/secNA 0.25 C (35–99.9 C)0–100.0 C 0.5 C (30 sec after reaching 95 C)27.2 x 33.0 x 56.5 cm (10.6 x 13 x 22 in.)18.8 kg (41 lb)20.4 kg (45 lb)10–80 µL10–100 μL33 nLUSB, onboard8.4 inch color TFT LCD100–240 V, 50–60 Hz, max. 950 VA2 temperature zones6 temperatureper blockzones, 25 C rangeNA(5 C zone-to-zone)(5 C zone-to-zone)Cloud or mobile via Ethernet or Wi-Fi2 x 384-well,0.02 mLHigh throughput in384-well format3.0 C/sec1.6 C/sec5–20 µL* OpenArray Plate is compatible with the QuantStudio 12K Flex Real-Time PCR System.** 20K Chip is compatible with the QuantStudio 3D Digital PCR System.† The Instrument Connect app, available at Apple and Google app stores, can be used to monitor your instrument. The Connect Platform can be used to create and share protocols, schedule an instrument, andstart and monitor runs remotely.Ordering informationProductComplete systemCat. No.Block onlyCat. No.Instrument 5-year warrantywith Rapid Exchange Cat. No.ProFlex 96-Well PCR System44840754483637A27934ProFlex 3 x 32-Well PCR System44840734483638A28986ProFlex 2 x 96-Well PCR System44840764484071A27937ProFlex 2 x Flat PCR System44840784484074A27931ProFlex 2 x 384-Well PCR System44840774484072A30229Recommended plastics3 x 32-well blockCat. No.96-well blockCat. No.384-well blockCat. No.MicroAmp TriFlex 3 x 32-WellPCR Reaction PlateA32811MicroAmp EnduraPlate Optical96-Well Clear Reaction Platewith Barcode4483354MicroAmp Optical 384-WellReaction Plate4343370MicroAmp 8-Tube StripA30589with Attached Domed Caps, 0.2 mLMicroAmp Reaction TubeN8010540with Cap, 0.2 mLMicroAmp TriFlex 3 x 32-Well PCRReaction PlateMicroAmp 8-Tube Strip withAttached Domed Caps, 0.2 mLA328114309849MicroAmp 32-Well ClearAdhesive FilmMicroAmp Clear Adhesive Film4306311MicroAmp Optical 384-WellReaction Plate with BarcodeMicroAmp EnduraPlate Optical384-Well Clear Reaction Platewith BarcodeMicroAmp Clear Adhesive FilmMicroAmp 32-Well ClearAdhesive FilmA32812A32812A3058944832854306311Did you know?The ProFlex, VeritiPro, and SimpliAmp Thermal Cyclers feature VeriFlex temperature control technology,which enables more precise and efficient PCR optimization.Find out more at thermofisher.com/veriflextechnology7

Ultimate performanceVeritiPro Thermal CyclerThe Applied Biosystems VeritiPro Thermal Cycler deliversproven reliability with advanced temperature controltechnology and connectivity. Take advantage of next-levelPCR optimization with the precision offered by VeriFlextemperature control technology. Connect to the cloudenabled VeritiPro Thermal Cycler remotely. Convenientlydesign and securely upload your methods, monitor runs,and check instrument availability from any mobile device ordesktop computer with the Connect Platform. Innovative design—ramp rate of 6.0 C/sec, quiet fan,and ergonomic soft-close lid VeriFlex Block—precise control over six independenttemperature zones for PCR optimization cloud-enabled instrument Fleet control–compatible—manage multipleinstruments, users, and methods with Thermal CyclerFleet Control SoftwareTemperature zonesPrecise control with VeriFlex temperaturecontrol technologyVeriFlex Blocks are constructed of segmented metalblocks with separate heating/cooling elements beloweach, enabling: More precise control over PCR optimization—eachblock can be set with up to six specific temperatures Precise incubation—use the six temperature zonesof the VeriFlex Block to do enzyme studies, restrictiondigests, or any other process that requires precisetemperature controlFind out more at thermofisher.com/veritiproTired of water baths?Incubate samples at up to six different temperatures simultaneously for enzyme activation studies,restriction digests, or sequencing library preps with the VeritiPro Thermal Cycler.88

SpecificationsBlock format96-well, 0.2 mL, 6-zone VeriFlex Block384-well, 0.02 mL, isothermalMax. block ramp rate6.0 C/sec5.0 C/secMax. sample ramp rate4.4 C/sec3.5 C/sec 0.25 C (35–99.9 C)Temperature accuracy0–100.0 CTemperature rangeTemperature uniformity 0.5 C (30 sec after reaching 95 C)Dimensions (H x W x D)21.7 x 24.5 x 46.5 cm (8.5 x 9.6 x 18.3 in.)12.0 kg (26.5 lb)Weight10–100 μLPCR volume rangeUSB port and 16 GB onboard memory; onboard capacity 1,000 protocolsInstrument memory8-inch color TFT LCDDisplay interface100–240 V, 50–60 Hz, max. 700 WPowerVeriFlex Block range5–20 μL30 C range across block, 6 temperature zones (up to10 C zone-to-zone)Data connectivity*Not applicableCloud or mobile via Ethernet or Wi-Fi* The Instrument Connect app, available at Apple and Google app stores, can be used to monitor your instrument. The Connect Platform can be used to create and share protocols, schedule an instrument, andstart and monitor runs remotely.Ordering informationProductCat. No.Instrument 3-year warrantywith Rapid Exchange Cat. No.VeritiPro 384-Well Thermal CyclerA48140A50326VeritiPro 96-Well Thermal CyclerA48141A48765Recommended plastics384-well blockMicroAmp Optical 384-Well Reaction Plate4343370MicroAmp Optical 384-Well Reaction Plate with Barcode4309849MicroAmp EnduraPlate Optical 384-Well Clear Reaction Plate with Barcode4483285MicroAmp Clear Adhesive Film430631196-well blockCat. No.MicroAmp EnduraPlate Optical 96-Well Clear Reaction Plate with Barcode4483354MicroAmp Optical 96-Well Reaction PlateN8010560MicroAmp Clear Adhesive Film4306311MicroAmp TriFlex 3 x 32-Well PCR Reaction PlateA32811MicroAmp 32-Well Clear Adhesive FilmA32812MicroAmp 8-Tube Strip with Attached Domed Caps, 0.2 mLA305899

Elegantly simple and preciseSimpliAmp Thermal CyclerThe Applied Biosystems SimpliAmp Thermal Cycler isan easy-to-use, compact, and accurate thermal cyclerdesigned to fit every lab’s essential PCR workflow.Features like a responsive color touchscreen and VeriFlextemperature control technology enable simple, accurateoptimization. Plus, the SimpliAmp Thermal Cycler is cloudenabled, giving you the freedom to design and securelyupload your methods, monitor runs, and check instrumentavailability from any mobile device or desktop computerwith the Connect Platform. Intuitive interface—large, easy-to-use color touchscreenfor easy programming and quick status checks VeriFlex Block—three independent temperature zonesfor PCR optimization cloud-enabled instrument Cloud-enabled—conveniently access your instrumentanytime, anywhere with the Connect Platform Compact design—helps save bench space Fleet control–compatible—manage multipleinstruments, users, and methods with Thermal CyclerFleet Control SoftwareThe status dial displays thecurrent block temperature andelapsed run time.Simple, intuitive user interfaceThe SimpliAmp Thermal Cycler has an 8 inch colortouchscreen, making navigation of the intuitive menuoptions fast and efficient.Create a new runmethod using defaulttemplates or existingmethods.Select one of yourexisting run methodsto start a run.Use the instrument asa precise incubatorfor non-PCRworkflows.Find out more at thermofisher.com/simpliamp10

SpecificationsBlock formatFeatures96-well, 0.2 mL, 3-zone VeriFlex Block Enabled to run Fast chemistry Auto restart (after power outages) Controllable ramp rate Edit program during experiment Program overwrite protection One-touch incubationMax. block ramp rate*4 C/secMax. sample ramp rate*3 C/sec 0.25 C (35–99.9 C)Temperature accuracy0–100.0 CTemperature rangeTemperature uniformity 0.5 C (30 sec after reaching 95 C)Temperature calibrationCalibrated to standards traceable to the National Institute of Standards and Technology (NIST)Dimensions (H x W x D)21.0 x 24.0 x 46.0 cm (8.3 x 9.5 x 18.1 in.)8.3 kg (18.3 lb)WeightPCR volume range10–100 μLInstrument memory2,000 MB onboard memory (capacity for 1,000 protocols); USB port for additional externalstorage8 inch color TFT LCDDisplay interface100–240 V, 50–60 Hz, max. 600 WPowerVeriFlex Block3 temperature zones, 20 C range (10 C zone-to-zone)Data connectivity**Cloud or mobile via Ethernet or Wi-Fi* At reaction volume of 1 μL.** The Instrument Connect app, available at Apple and Google app stores, can be used to monitor your instrument. The ConnectPlatform can be used to create and share protocols, schedule an instrument, and start and monitor runs remotely.Ordering informationProductCat. No.Instrument 5-year warrantywith Rapid Exchange Cat. No.SimpliAmp Thermal CyclerA24811A27603High-Power USB Wi-Fi ModuleA26774NARecommended plastics96-well blockCat. No.MicroAmp EnduraPlate Optical 96-Well Clear Reaction Plate with Barcode4483354MicroAmp Optical 96-Well Reaction PlateN8010560MicroAmp Clear Adhesive Film4306311MicroAmp TriFlex 3 x 32-Well PCR Reaction PlateA32811MicroAmp 32-Well Clear Adhesive FilmA32812MicroAmp 8-Tube Strip with Attached Domed Caps, 0.2 mLA30589Did you know?ProFlex, VeritiPro, SimpliAmp, and MiniAmp Thermal Cyclers have thermal simulation modes that makethe transition from other thermal cyclers simple, accurate, and efficient. A library of modes that mimicthe ramp rates of other instruments is available on each instrument.11

Routine PCRMiniAmp Thermal CyclerThe Applied Biosystems MiniAmp Thermal Cyclerdelivers the reliability you’ve come to expect from AppliedBiosystems technology for routine PCR at every lab bench. Compact design—fits in most lab spaces at just 7.5 in.(19 cm) wide Cloud-enabled—conveniently access your instrumentanytime, anywhere with the Connect Platform and amobile device Fleet control–compatible—manage multipleinstruments, users, and methods with Thermal CyclerFleet Control Software cloud-enabled instrumentSecure remote access withcloud-enabled instrumentsThe ProFlex, VeritiPro, SimpliAmp, and MiniAmp ThermalCyclers are cloud-enabled instruments, allowing yousecure, private access with an account on the ConnectPlatform. Anywhere, anytime with any mobile device ordesktop computer you can: Design and share protocols Schedule an instrument Start or stop a run Check run statusFind out more at thermofisher.com/miniampDid you know?Most Applied Biosystems thermal cyclers come with a two-year standard warranty and a starterkit that includes Applied Biosystems PCR plate and tube samples and all the tools you need.Packages that include the instrument, an extended warranty, and consumables are available.Visit the instrument webpage to view available packages.12

SpecificationsMiniAmp Thermal CyclerMiniAmp Plus Thermal CyclerBlock format96-well, 0.2 mL isothermal block96-well, 0.2 mL, 3-zone VeriFlex BlockMax. block ramp rate*3.0 C/sec3.5 C/secMax. sample ramp rate*2.2 C/sec2.7 C/sec 0.25 C (35–99.9 C)Temperature accuracy0–100.0 CTemperature rangeTemperature uniformity 0.5 C (30 sec after reaching 95 C)Temperature calibrationCalibrated to standards traceable to the National Institute of Standards and TechnologyDimensions (H x W x D)20 x 19 x 39 cm (7.9 x 7.5 x 15.4 in.)5.9 kg (13.0 lb)WeightPCR volume range10–100 μLInstrument memory2,000 MB onboard memory (capacity for 1,000 protocols);USB port for additional external storage5 inch color TFT LCDDisplay interface100–240 V, 50–60 Hz, max. 500 WPowerVeriFlex Block3 temperature zones, 20 C range(10 C zone-to-zone)NAData connectivity**Cloud or mobile via Ethernet or Wi-Fi* At reaction volume of 1 μL.** The Instrument Connect app, available at Apple and Google app stores, can be used to monitor your instrument. The Connect Platform can be used to create and share protocols, schedule an instrument, andstart and monitor runs remotely.Ordering informationProductCat. No.Instrument 5-year warrantywith Rapid Exchange Cat. No.MiniAmp Thermal CyclerA37834A38081MiniAmp Plus Thermal CyclerA37835A38077High-Power USB Wi-Fi ModuleA26774NARecommended plastics96-well blockCat. No.MicroAmp EnduraPlate Optical 96-Well Clear Reaction Plate with Barcode4483354MicroAmp Optical 96-Well Reaction PlateN8010560MicroAmp Clear Adhesive Film4306311MicroAmp TriFlex 3 x 32-Well PCR Reaction PlateA32811MicroAmp 32-Well Clear Adhesive FilmA32812MicroAmp 8-Tube Strip with Attached Domed Caps, 0.2 mLA30589Want to learn more about thermal cycler ramp rates and how they are calculated?Go to thermofisher.com/ramprate13

Designed for easy robotic integrationAutomated Thermal CyclerThe Applied Biosystems Automated Thermal Cycleroffers the flexibility, reliability, and performance needed ina complete PCR automation system. The small, easy-tointegrate format of the Automated Thermal Cycler enableshands-free PCR results. Flexible modular design and small footprint—helpssave space on deck Automated lid—easy, hands-free operation with a liquidhandler or plate stacker Free desktop software—for PCR optimization prior torobotic integration automation-ready instrument Plug-and-play drivers and Standardization in LabAutomation (SiLA) compatibility—for easy integrationon your liquid handler of choice Fleet control–compatible—manage multipleinstruments, users, and methods with Thermal CyclerFleet Control SoftwareDesigned for any stage of your workflow automation journeyStand-alonePlug-and-play driversSiLA-compatibleOptimize assays beforerobotic integration with ourdirect software.Ask about available drivers forleading robotic platforms.Maximum robotic platformintegration flexibility with SiLArapid integration coding.Find out more at thermofisher.com/atc14

SpecificationsBlock formatPCR volume rangeHardware integration features96-well, 0.2 mL*384-well, 0.02 mL10–100 μL for full-skirted plates; 20–100 µL for5–20 µL for full-skirted platessemi-skirted plates Predrilled mounting and/or alignment points at each corner of the chassis 3-side and top-plate access Available in 3-connector configurations Application programming interfaces (APIs) available for integration with robotics systemsSoftware featuresBlock module dimensions (H x W x D)Control module dimensions (H x W x D)Temperature accuracyMax. block ramp rate†Max. sample ramp rate†Temperature rangeTemperature uniformityTemperature calibrationService optionsWeightPowerFlexible ramp ratesData connectivity SiLA Rapid Integration software–standardized programming access** Free software available for instrument demonstration and stand-alone operation13.3 x 17.9 x 31.7 cm (5.2 x 7.0 x 12.5 in.)7.0 x 25.7 x 33.1 cm (2.8 x 10.1 x 13.0 in.) 0.25 C (35.0–99.9 C)3.5 C/sec2.8 C/sec1.8 C/sec1.6 C/sec4–105 C (no condensation risk with sub-ambient temperatures) 0.50 C (20 sec after reaching 95 C)Calibrated to standards traceable to the National Institute ofStandards and Technology (NIST) 2-year standard warranty includes Rapid Exchange Service plan NIST-traceable temperature probe equipment available9.4 kg/20.7 lb total (block module 6.0 kg/13.2 lb, control module 3.4 kg/7.5 lb)100–240 V, 50–60 Hz, max. 600 WProgram your own ramp rates, or use preprogrammed simulation modesLAN* Compatible with full- or semi-skirted plates. ATC semi-skirted adaptor required for use with semi-skirted 96-well plates. ATC ships with the full-skirted adaptor installed, which is required for use with full-skirted96-well plates.** sila-standard.org† At reaction volume of 1 µL.Ordering informationProductAutomated Thermal Cycler System, laptop, 1 m cableAutomated Thermal Cycler System, laptop, 3 m cableAutomated Thermal Cycler System, laptop, 10 cm cableAutomated Thermal Cycler System, 1 m cableAutomated Thermal Cycler System, 3 m cableAutomated Thermal Cycler System, 10 cm cableAutomated Thermal Cycler Semi-Skirted AdaptorAutomated Thermal Cycler Full-Skirted Adaptor96-well Cat. 5384-well Cat. No.A33977A33978A33979A33980A33981A33982NANA3-year extended warranty* withRapid Exchange Cat. No.ZGEXSCATC3Y/ZGEXSCATC3843YNANA* The Automated Thermal Cycler comes standard with a 27-month warranty.Recommended plastics96-well blockMicroAmp EnduraPlate Optical 96-Well Full-SkirtedPlate with Barcode, Clear*MicroAmp EnduraPlate Optical Semi-Skirted Platewith Barcode**MicroAmp Clear Adhesive FilmCat. No.A3172844833564306311384-well blockMicroAmp EnduraPlate Optical 384-Well Clear ReactionPlate with BarcodeMicroAmp EnduraPlate Optical 384-Well MulticolorReaction Plate with BarcodeMicroAmp Clear Adhesive FilmCat. No.448327344833174306311* Available in multiple colors.** Requires semi-skirted plate adaptor (Cat. No. A33044); included with instrument purchase.The Applied Biosystems MicroAmp EnduraPlate plate (Cat. No. A31728) was specifically designedfor the Automated Thermal Cycler and has three-way barcoding. Learn about the amplificationuniformity benefits at thermofisher.com/automatedPCRplate1515

Manage multiple users, methods, andinstruments with a single intuitive interfaceThermal Cycler Fleet Control SoftwareApplied Biosystems Thermal Cycler Fleet Control Softwareis as intuitive and easy to use as our thermal cyclers are.With this tool, you’ll be able to view and control all AppliedBiosystems thermal cyclers in your facility.Secure—comes preinstalled on a server that sits securelybehind your firewallFlexible—create custom permissions and rules for users,methods, and instrumentsPowerful—quickly view your thermal cycler inventory andavailability right from the dashboardEfficient—track system changes with an audit log, andidentify methods or instruments via barcode data entryConvenient—control your thermal cyclers, reports,methods, and firmware upgrades from your office, anotherlab computer, or any other networked deviceFind out more at thermofisher.com/fleetcontrolKeep working wonders—we’ve got your backCount on superior services and support to keep your thermal cyclers up and runningYou can’t afford downtime in your lab. Built on more than40 years of service expertise, our superior service solutionsfor Applied Biosystems instruments and applications helpkeep your lab up and running. More than 2,000 trainedprofessionals make up the industry’s largest network, readyto assist you when you need it.Rapid ExchangeWhen fast turnaround time is critical and asset tagconsistency is not required, choose our Rapid Exchangeplan. If our Remote Service Center engineers can’t repairyour instrument through telephone support, we will shipyou a factory-certified refurbished replacement instrumentwithin one business day—and it’s yours to keep.1Receive a replacement unit (your existingservice coverage now applies to this instrument)2Package the broken instrument in the same box3Ship it back to us free of chargeAB Repair Center (ABRC) Support PlusOur AB Repair Center plan is the best choice for customersin regulated environments that must maintain asset tagconsistency to comply with regulated protocols and needtheir instrument returned after repair. With this mail-inOff-site service plans at a glanceRapid ExchangeAB Repair Center (ABRC) Support PlusPlan designed forLabs that operate under critical timelinesRegulated labs that must maintain asset tagconsistency to comply with regulated protocolsRepair timeReplacement shipped in 1 business day*Loaner instrument shipped in 1 business day**Off-site repair service including shipping,parts, and labor Telephone and email access to ourRemote Service Center * Subject to regional availability.** Your instrument repaired and returned in 3 weeks. Service plan without loaner instrument also available.16

option, we provide a loaner instrument for you to usewhile factory-trained engineers maintain and repair yourinstrument at our Remote Service Center. All instrumentsundergo certification performance tests, temperatureverification, instrument and optical calibration, groundcontinuity, and firmware upgrades, when applicable.Watch the “Five reasons to verify your thermal cycler’saccuracy” video at thermofisher.com/mdtvDownload a sample of an MDTV reportat thermofisher.com/mdtvreportEducation servicesIt can be difficult to prepare yourself for what’s nextwhile you’re focused on the work you have now.Our interactive professional training courses make iteasier. We offer a combination of virtual and in-personclassroom instruction, and hands-on learning in yourlab to match your schedule, budget, and learningpreferences. Whichever course style you choose, you’lllearn from one of our 300 highly skilled applicationscientists who are available to lead sessions online,at your location, or at one of our 12 training centerslocated worldwide.Just mail us your instrument and we’ll repair and shipit back to you within three weeks. All parts, labor, andshipping charges are included. Planned maintenance,temperature verification, and loaner instrument service* canbe added to any off-site service plans.Instrument service coverage lookupCheck your instrument’s coverage status atthermofisher.com/manageDigital service innovationsOur instrument service plans include digital innovations thathelp keep your instruments and your lab running smoothly.With pioneering on-demand tools and capabilities suchas remote support using mobile augmented-reality (AR)technology, instrument-driven support, and on-demandinstrument training, we’re constantly looking ahead so yourlab doesn’t fall behind.Explore courses atthermofisher.com/educationservicesTechnical supportIf you have questions about product selection or use, assayor experimental design, data analysis, or troubleshooting,contact our team of technical support scientists or accessour online product and application support tools.Explore our digital service innovations atthermofisher.com/innovationsHow to reach usTo find your local support or technical support team, go tothermofisher.com/contactusTemperature verification servicesMultiplex dynamic temperature verification (MDTV) andmultiplex dynamic temperature measurement (MDTM)services for Applied Biosystems thermal cyclers** aredesigned to deliver fast, accurate temperature readingsand documentation to assess the accuracy and uniformityof your PCR systems.For product FAQs, protocols, training courses, andwebinars, go to thermofisher.com/technicalresources* Loaner instruments are not available in all regions. Contact your services and support representativefor availability.** MDTV service not currently offered on the 60-well Applied Biosystems GeneAmp PCRSystem 9700.5-channelmultiplexelectronicsUSBMDTV/M probes97002720ProFlexFind out more at thermofisher.com/thermalcyclerservice17

High-performance PCR plastics for optimal PCR resultsEngineer Approved MicroAmp PCR plasticsApplied Biosystems PCR plastics have been designedand validated to work with our thermal cyclers for morethan 25 years. That’s why they are Engineer Approvedto enable optimal PCR performance.Applied Biosystems MicroAmp PCR plastics are: Validated on Applied Biosystems thermal cyclers foroptimal fit and performance Designed for optimal heat transfer with thin-walledpolypropylene wells Designed to reduce cross-contamination with raisedwell rims for effective sealingApplied Biosystems MicroAmp TriFlex 3 x 32-WellPCR Reaction Plate and adhesive film One plate that can be used as separated, individual32-well plates or as a full 96-well plate Easy-to-tear dual side tabs Alphanumeric labeling on each plate segment Precut Applied Biosystems MicroAmp 32-Well ClearAdhesive FilmUnique, high-performance features of Applied Biosystems MicroAmp EnduraPlate plastic consumablesEasy visual organization5 color choicesConstructed to ANSI/SBS standardEasy-to-read well identification textBlack text for excellent contrastNo warping, even afterthermal cyclingPolycarbonate (hard shell) formechanical stability and flatnessCertified DNA-, RNase-, andPCR inhibitor–freeCompatible with andoptimized for performance onApplied Biosystems instrumentsSnug fit to thermal blocksThin-wall polypropylene for excellentmechanical fit and heat transferAvailable in common formats96-well standard, 96-well Fast, 384-well,5-piece sample packs, 20 & 500 packs18

Options for every format and throughput needChoose from tubes, tube strips, plates, adhesive film,and accessories for any throughput need. MicroAmpEnduraPlate plastics offer a solution for experimentsthat require special handling, such as automated orhigh-throughput workflows, and an even greater degreeof durability for use with multi-instrument experiments.The Applied Biosystems MicroAmp 8-Tube Strip withattached optical or domed caps offers a combination offeatures to help prevent cross-contamination, pipettingerrors, and sample identification errors in your PCR andreal-time PCR applications. Attached caps that open and close independently ofeach other Etched A–H letter labeling for individual tubes and capsMicroAmp 8-tube strips with attached optical flat caps are alsoavailable for qPCR. Dual side tabs for strip labeling Graduated 20 μL measuring markers on every tubeFind out more atthermofisher.com/findplastics Available exclusively for Applied Biosystems thermalcyclers to enable optimal PCR resultsDid you know?Proper plate sealing helps reduce evaporation and well-to-well contamination

Data connectivity† Cloud or mobile via Ethernet or Wi-Fi * OpenArray Plate is compatible with the QuantStudio 12K Flex Real-Time PCR System. ** 20K Chip is compatible with the QuantStudio 3D Digital PCR System. † The Instrument Connect app, available at Apple and Google app stores, can be used to monitor your instrument. The Connect .