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Schedule of Accreditationissued byUnited Kingdom Accreditation Service2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UKSt George’s University Hospitals NHS Foundation TrustIssue No: 003 Issue date: 13 April 20219913Accredited toISO 15189:2012St George's Healthcare NHS TrustContact: Robin WhittakerCellular PathologyTel: 44 (0) 20 8725 2840Blackshaw RoadFax: 44 (0) 20 8767 7984LondonE-Mail: robin.whittaker@stgeorges.nhs.ukSW17 0QTWebsite: www.stgeorges.nhs.ukTesting performed by the Organisation at the locations specified belowLocations covered by the organisation and their relevant activitiesLocation detailsActivityLocationcodeSt George's Healthcare NHS TrustCellular PathologyBlackshaw RoadLondonSW17 0QTLocal Contact:Robin WhittakerAs aboveRoutine HistologyDigital PathologyFrozen section serviceSpecial stainsImmunocytochemistryEnzyme histochemistryMolecular testingCytology –non-gynaeSGHCroydon University Hospital530 London RoadCroydonCR7 7YELocal Contact:Robin WhittakerAs aboveOSNAFrozen section (Mohs)CUHAssessment Manager: EWSPage 1 of 21

S c he dul e of Ac c re di ta ti onissued byUnited Kingdom Accreditation Service2 Pine Trees, Chertsey Lane, Staines -upon-Thames, TW18 3HR, UKSt George’s University Hospitals NHS Foundation Trust9913Accredited to15189:2012ISOIssue No: 003Issue date: 13 April 2021Testing performed at main address onlySite activities performed away from the locations listed above:Location detailsHead and Neck FNA clinicsUltrasound Room St James Wing X-RayDepartmentLocal Contact:Robin WhittakerAs aboveActivityLocationcodeFNA material/slide preparationCellularity assessmentSGHUltrasound Lanesborough Wing ScanningDepartmentMelanoma clinic at The Rose CentreSt George’s Healthcare NHS trustBlackshaw RoadLondonEBUS – Endobronchial Ultrasound clinic,H&N Clinic, Interventional radiology suiteCUHCroydon University Hospital530 London RoadCroydonCR7 7YEAssessment Manager: EWSPage 2 of 21

S c he dul e of Ac c re di ta ti onissued byUnited Kingdom Accreditation Service2 Pine Trees, Chertsey Lane, Staines -upon-Thames, TW18 3HR, UKSt George’s University Hospitals NHS Foundation Trust9913Accredited to15189:2012ISOIssue No: 003Issue date: 13 April 2021Testing performed at main address onlyDETAIL OF ACCREDITATIONMaterials/ProductstestedHUMAN BODY TISSUE /FLUIDSFresh and formalin fixedtissue samplesType of test/Propertiesmeasured/Range ofmeasurementRoutine Histopathologyexaminations to assist inclinical investigations anddiagnosisStandard specifications/Equipment/Techniques usedLocationCodeMacroscopic and Microscopicexamination using in-houseprocedures (listed) in conjunction withmanufacturer’s instructions for thefollowing methods (where relevant)Macroanalysis and dissection using:SGHCEL-HIS-LAB-005 Specimen cut upCEL-MED-CUT-001 Medical protocolfor specimen cut upCEL-MED-CUT-006 Protocol for Cutup and Reporting of ThoracicSpecimensCEL-MED-CUT-007 Medical Protocolfor Cut-Up of Specimens from theCardiovascular SystemCEL-MED-CUT-010 Medical Protocolfor Cut-Up Of AppendicectomySpecimensCEL-MED-CUT-011 Medical Protocolfor Dealing with ColorectalPolypectomiesCEL-MED-CUT-012 Medical Protocolfor Cut-Up Colorectal CancerSpecimensCEL-MED-CUT-013 Medical Protocolfor Cut-Up Of Completion ProctectomySpecimensCEL-MED-CUT-014 Medical Protocolfor Cut-Up Of CholecystectomySpecimensCEL-MED-CUT-015 Medical protocolfor cut-up of urological specimensCEL-MED-CUT-016 Medical Protocolfor Cut-Up Endocrine (Adrenal andParathyroid) Specimens and TissuePathways for ReportingCEL-MED-CUT-017 GynaecologicalPathology Specimen DissectionCEL-MED-CUT-018 Medical Protocolfor Cut-Up of Colectomy Specimensfor Inflammatory Bowel DiseaseAssessment Manager: EWSPage 3 of 21

S c he dul e of Ac c re di ta ti onissued byUnited Kingdom Accreditation Service2 Pine Trees, Chertsey Lane, Staines -upon-Thames, TW18 3HR, UKSt George’s University Hospitals NHS Foundation Trust9913Accredited to15189:2012ISOIssue No: 003Issue date: 13 April 2021Testing performed at main address onlyMaterials/ProductstestedHUMAN BODY TISSUE /FLUIDS (cont’d)Fresh and formalin fixedtissue samplesType of test/Propertiesmeasured/Range ofmeasurementRoutine Histopathologyexaminations to assist inclinical investigations anddiagnosis (cont’d)Standard specifications/Equipment/Techniques usedLocationCodeMacroscopic and Microscopicexamination using in-houseprocedures (listed) in conjunction withmanufacturer’s instructions for thefollowing methods (where relevant)Macroanalysis and dissection (cont’d)using:CEL-MED-CUT-020 Medical Protocolfor Cut-Up of Paediatric BowelSpecimens in Hirschsprung’s DiseaseCEL-MED-CUT-021 Medical Protocolfor Paediatric Tumour Specimen CutUpSGHCEL-MED-CUT-022 Medical Protocolfor Cut-Up Of PlacentaeCEL-MED-CUT-023 Medical Protocolfor Cut-Up of Breast SpecimensCEL-MED-CUT-024 Medical Protocolfor Cut-Up of Lymph Node SpecimensCEL-MED-CUT-025 Medical Protocolfor Cut-Up of NeuropathologySpecimensCEL-HIS-APX-006 Routine Usage ofthe VANTAGE Workflow SolutionTracking SystemFormalin fixed tissuesincluding cell blocksTissue ProcessingAutomated method using:CEL-HIS-EQP-007 Tissue-Tek VIP6AICEL-HIS-LAB-024 Re-processingCEL-HIS-LAB-012 Fresh PaediatricTissuesCEL-HIS-LAB-013 Fresh SurgicalTissuesSGHFormalin fixed, processedtissues and cell blocksTissue EmbeddingManual method using:CEL-HIS-LAB-015Sakura Tissue Tek embedding centresCEL-HIS-EQP-008 Operation andMaintenance of the Tissue Tek Embedding CentresSGHAssessment Manager: EWSPage 4 of 21

S c he dul e of Ac c re di ta ti onissued byUnited Kingdom Accreditation Service2 Pine Trees, Chertsey Lane, Staines -upon-Thames, TW18 3HR, UKSt George’s University Hospitals NHS Foundation Trust9913Accredited to15189:2012Issue No: 003ISOIssue date: 13 April 2021Testing performed at main address onlyMaterials/ProductstestedHUMAN BODY TISSUE /FLUIDS (cont’d)Type of test/Propertiesmeasured/Range ofmeasurementRoutine Histopathologyexaminations to assist inclinical investigations anddiagnosis (cont’d)Formalin fixed, paraffinembedded (FFPE) tissueand cell blocksStandard specifications/Equipment/Techniques usedLocationCodeMacroscopic and Microscopicexamination using in-houseprocedures (listed) in conjunction withmanufacturer’s instructions for thefollowing methods (where relevant)SGHMicrotomyManual sectioning using:CEL-HIS-LAB-006Leica 2135 and Sakura SRM 200Accucut microtomesSGHFresh / frozen tissueIntra-operative diagnosis ofbasophilic and eosinophilictissue structureRapid frozen sectionCryotomy, automated and manualHaematoxylin & Eosin (H&E) stainingmethod using Leica CM1850 andLeica CM1860 UV CryostatsCEL-HIS-EQP-004 Operation andMaintenance of the Leica CM1850 &CM1860 UV CryostatCEL-HIS-LAB-030 CryotomyCEL-HIS-LAB-004 Freezing tissuesCEL-HIS-LAB-012 Paediatric tissueCEL-HIS-LAB-013 Surgical tissueCEL-HIS-LAB-017 Infective tissueCEL-HIS-LAB009 Handling andorientation of muscle biopsiesSGHFresh / frozen tissueDetection of:IgAIgMComplementManual ImmunofluorescenceCEL-HIS-LAB-026 Skin SpecimensRequiring ImmunofluorescenceCEL-ICC-GEN-008Fluorescence microscopyZeiss Axioplan 2SGHFresh / frozen tissue (skinand mucosa)Frozen Section Histologyexaminations to assist inclinical investigationsCEL-HIS-LAB-013 MohsLeica CM1850 cryostatShandon Linistain – Automated H&EstainingOlympus BX41 microscopeCEL-MOH-GEN-001 Mohs TechniqueCUHAssessment Manager: EWSPage 5 of 21

S c he dul e of Ac c re di ta ti onissued byUnited Kingdom Accreditation Service2 Pine Trees, Chertsey Lane, Staines -upon-Thames, TW18 3HR, UKSt George’s University Hospitals NHS Foundation Trust9913Accredited to15189:2012Issue No: 003ISOIssue date: 13 April 2021Testing performed at main address onlyMaterials/ProductstestedType of test/Propertiesmeasured/Range ofmeasurementHUMAN BODY TISSUE /FLUIDS (cont’d)Special staining ofHistopathology examinationsto assist in detection of clinicalabnormalitiesManual tinctorial and histochemicalmethods using:SGHSlides prepared fromsamples aboveIdentification of basophilic andeosinophilic tissue structuresand diastase resistantpolysaccharidesRoutine Haematoxylin & Eosin (H&E)and PAS /- D staining andcoverslippingAutomated method using:CEL-HIS-EQP-003Sakura Tissue Tek Prisma StainerShandon LinistainerSGHSections (slides) fromFFPE tissue / cell blocksAcid and neutral mucinsAlcian Blue PAS CEL-HIS-STA-003Acid mucopolysaccharideAlcian Blue CEL-HIS-STA-002AmyloidCongo Red CEL-HIS-STA-008Diastase resistantpolysaccharidesPAS /- Diastase CEL-HIS-STA-010GlycogenABPASD CEL-HIS-STA-004Connective tissueElastic Van Geison CEL-HIS-STA-011H. pylori and H. pylori likeorganismsGiemsa CEL-HIS-STA-013Bone canaliculaeGiemsa – Bone marrow CEL-HIS-STA012Gram Stain CEL-HIS-STA-014Gram positive and negativebacteriaAssessment Manager: EWSStandard specifications/Equipment/Techniques usedGram positive and negativebacteriaGram Twort CEL-HIS-STA-015Basement membrane, fungiGrocott CEL-HIS-STA-017Cellular ComponentsHaematoxylin and Eosin CEL-HISSTA-018LocationCodePage 6 of 21

S c he dul e of Ac c re di ta ti onissued byUnited Kingdom Accreditation Service2 Pine Trees, Chertsey Lane, Staines -upon-Thames, TW18 3HR, UKSt George’s University Hospitals NHS Foundation Trust9913Accredited to15189:2012Issue No: 003ISOIssue date: 13 April 2021Testing performed at main address onlyMaterials/ProductstestedHUMAN BODY TISSUE /FLUIDS (cont’d)Sections (slides) fromFFPE tissue / cell blocksAssessment Manager: EWSType of test/Propertiesmeasured/Range ofmeasurementStandard specifications/Equipment/Techniques usedCollagen, muscle and bilepigmentsSpecial staining ofHistopathology examinationsto assist in detection of clinicalabnormalities (cont’d)HVG CEL-HIS-STA-019Connective tissueMSB CEL-HIS-STA-023MelaninMasson Fontana CEL-HIS-STA-021Connective tissueHepatitis B surface antigenand copper associated proteinMasson Trichrome CEL-HIS-STA-022Orcein CEL-HIS-STA-027Fungi, glycogen, neutral mucinand basement membraneGlycogenPAS CEL-HIS-STA-028HaemosiderinPerls CEL-HIS-STA-0Reticulin fibresReticulin CEL-HIS-STA-034CopperRhodanine CEL-HIS-STA-033AmyloidThioflavin T CEL-HIS-STA-040Mast cell granules and acidmucinToluidine Blue CEL-HIS-STA-026Calcium depositsVon Kossa CEL-HIS-STA-036Leprosy bacilliWade Fite CEL-HIS-STA-037Spirochetes, HelicobacterWarthin-Starry CEL-HIS-STA-041MycobacteriaZiehl-Neelson CEL-HIS-STA-039Manual tinctorial and histochemicalmethods using:LocationCodeSGHPAS /- D – Muscle method CEL-HISSTA-051Page 7 of 21

S c he dul e of Ac c re di ta ti onissued byUnited Kingdom Accreditation Service2 Pine Trees, Chertsey Lane, Staines -upon-Thames, TW18 3HR, UKSt George’s University Hospitals NHS Foundation Trust9913Accredited to15189:2012Issue No: 003ISOIssue date: 13 April 2021Testing performed at main address onlyMaterials/ProductstestedType of test/Propertiesmeasured/Range ofmeasurementStandard specifications/Equipment/Techniques usedHUMAN BODY TISSUE /FLUIDS (cont’d)Enzyme HistochemistryPreparation and examinationto identify or excludemorphological & cytologicalabnormalitiesManual histochemical methods using:Fresh / frozen tissueAcid phosphataseAcid Phosphatase CEL-HIS-STA-043Fresh / frozen rectalbiopsy tissueAcetylcholinesterase – nervecellsAcetylcholinesterase CEL-HIS-STA005 for Diagnosing Hirschsprung’sMuscle fresh/frozensectionsCytochrome oxidase activityCOX-SDH CEL-HIS-STA-044Muscle fresh/frozensectionsType 1 and 2 muscle fibresMyoadenylate Deaminase CEL-HISSTA-052Muscle fresh/frozensectionsTetrazolium reductase activityNicotinamide adenosine CEL-HISSTA-049Muscle fresh/frozensectionsPhosphorylase activityPhosphorylase CEL-HIS-STA-048Muscle fresh/frozensectionsMuscle, mitochondria andcollagenGomori Trichrome CEL-HIS-STA-047Muscle fresh/frozensectionsLipidsOil Red O CEL-HIS-STA-025Assessment Manager: EWSLocationCodeSGHPage 8 of 21