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next generation sequencingxGen NGS product catalogIDT aims to deliver innovative, high-performing NGS products to enableyour discovery power. Investigate how the varied IDT solutions can propelyour NGS research forward.Library preparationHybridization captureAmplicon sequencingLibrary normalizationW H Y x G E N N EXT GE N E R ATION SEQ U ENC ING?xGen NGS represents our commitment to accelerating your next breakthrough scientific discovery. Uncover acomprehensive, flexible range of solutions designed to help you sequence samples and generate research data younever thought you could.x G E N L I B R ARY P R E PAR ATIONxGen Library Prep Kit selectionCatalog #ReactionsEZ DNA Library Prep KitEnzymatic fragmentation of dsDNA. Indexing by PCR workflow.10009863161000982196EZ DNA Library Prep Kit UNIEnzymatic fragmentation. Indexing by ligation workflow.10009864161000982296MC DNA Library Prep KitMechanical fragmentation. Indexing by PCR workflow.10009861161000981996MC DNA Library Prep Kit UNIMechanical fragmentation. Indexing by ligation workflow.10009862161000982096MC DNA Library Prep Kit UNIMechanical fragmentation. Indexing by ligation workflow.10006202161000620396cfDNA & FFPE DNA Library Prep KitUse degraded and low-input research samples for variant detection.10009859161000981796ssDNA & Low-Input DNA Library Prep KitEnables preparation of libraries from degraded and damaged ssDNA & dsDNA samples.10009860161000982496Methyl-Seq Library Prep KitUtilizes Adaptase technology to construct libraries post-bisulfite conversion, to empowerepigenetic research.10009866161000981496RNA Library Prep KitA fast, low-cost transcriptome sequencing workflow for Illumina platforms.10009866161000981496Broad-Range RNA Library Prep KitCompatible with a range of input types, quantities, and indexing options for your research.10009865161000981396 WWW.IDTDNA.COM
next generation sequencingx G E N A D AP TE R S & IN D E XIN G PRIMERSSingle indexP5SP1InsertSP2i7P7Unique dual indexP5i5SP1InsertSP2i7P7Dual index UMIP5i5SP1InsertSP2i7UMI AP7Flow cell binding sequence: Platform-specific sequences for library binding to instrumentSequencing primer sites: Binding sites for general sequencing primersSample indexes: Short sequences specific to a given sample libraryMolecular index/barcode: Short sequence used to uniquely tag each molecule in a given sample libraryInsert: Target DNA or RNA fragment from a given sample libraryFigure 1. Adapter designs. A variety of NGS adapter designs are available from IDT. When selecting or designing adapters, consideration mustbe given to the intended application, multiplexing needs, specification requirements, and analysis methods.xGen Custom Adapters & PrimersCatalog #TruSeq – Compatible Full-length AdaptersAvailable in single index, combinatorial dual index, UDI, & UDI-UMI configurations.Request onlineTruSeq – Compatible Stubby Adapter and Indexing PrimersAvailable in single index, combinatorial dual index, & UDI configurations.Request onlineTruSeq – Compatible Indexing PrimersAvailable in single index, combinatorial dual index, & UDI configurations.Request onlineNextera – Compatible Indexing PrimersAvailable in single index, combinatorial dual index, & UDI configurations.Request online TruSeq and Nextera are registered trademarks of Illumina, Inc., and are used with permission. All rights reserved.xGen Indexing PrimersStubby AdaptersStubby Adapters and UDI PrimersUDI Primer PairsCDI PrimersCatalog ## of uniqueprimer 76168 nt10005921968 nt10005975168 nt10005922968 nt Plate 110009816968 nt Plate 21000805238410 nt Plates 1-41000805376810 nt Plates 1-810008054153610 nt Plates 1-161000805538410 nt Plates 1-4, 2 nmol*1000805676810 nt Plates 1-8, 2 nmol*10008057153610 nt Plates 1-16, 2 nmol*10009815968 nt* Bulk number of reactions will vary depending on quantity of primers added.xGen Full-Length AdaptersUDI-UMI Adapters2xGen NGS–Made for youCatalog #Reactions10006914161000590396
next generation sequencingFull-lengthadaptersIndexing strategyUDI UMIIndexing primer optionsUDIUDI stubbyCDINormalaseUDINormalaseCDIxGen RNA Library Prep xGen Broad-range RNA Library Prep xGen DNA Library Prep EZ or MC xGen DNA Library Prep EZ or MC UMI xGen ssDNA & Low-input DNA library Prep xGen Methyl-Seq Library Prep xGen cfDNA & FFPE Library Prep x G E N H Y B RIDIZ ATION CAP TURE PA NELSxGen Custom Hyb PanelsCatalog #ReactionsCustom Hyb PanelProvides the best trade-off between speed and value. NGS functional testing documentationincluded with a 3-week TAT*.Request online16, 96, & customCustom Hyb Panel—AccelHas fastest turnaround time for customers ( 5 days)*.Request online16, 96, & customCustom Hyb Panel—ProductionProvides additional quality and NGS functional testing documentation.Request online16, 96, & customCatalog #Reactions10005151410005152161000515396AML Cancer Hyb PanelTargets 260 genes associated with AML for efficient identification of ed Disease Hyb PanelTargets genes and single nucleotide polymorphisms (SNPs) associated withinherited diseases.101635216101635196Pan-Cancer Hyb PanelCaptures 127 mutated genes implicated across 12 tumor types for deeper sequencing.105620516105620496CNV Backbone Hyb PanelSpike-in panel that can be used to identify copy number variations in human NGSresearch data.108056316108056496Human ID Hyb PanelSpike-in panel that uses targeted enrichment of a definitive set of SNPs to identify aunique individual from a population of over 5 million biological samples.107570216107570396Human mtDNA Hyb PanelSpike-in panel for cost-effective identification of sequence variants in mitochondrial DNA.107570516107570696SARS-CoV-2 Hyb PanelSpans the complete viral genome and is not susceptible to known and novel mutationsarising from diverse strains.10006764961000698896** Turnaround time reflects up to 50,000 probes.xGen Predesigned Hyb PanelsExome Hyb PanelExome capture panel manufactured using GMP standards targeting 19,396 genes.* Includes SARS-CoV-2 Hyb Panel, hybridization wash kit, blockers, and library amplification primers.xGen NGS—Made for you3
next generation sequencingx G E N H Y B RIDIZ ATION CAP TURE C O RE REA GENT SxGen Hyb and Wash KitsHybridization and Wash KitsContains buffers, cot DNA & streptavidin beads required for capturexGen Blocking OligosTS Mix (for TruSeq -style library) 10 bp TS Mix (for TruSeq -style library) Standard Blocking OligosNXT Mix (for Nextera -style library)Ion Torrent (IT-P1 & A)Catalog #Reactions108057716108058496Catalog tom10Custom25Catalog #Size1080768150 µL1080769650 µLCatalog #Reactions1077675161077676961077677192TruSeq and Nextera are registered trademarks of Illumina, Inc., used with permission. All rights reserved.xGen Cot DNAHuman Cot DNAPlacental DNA enriched for repetitive DNA sequences. Used to block non-specific binding inDNA hybridization reactions.xGen Primer MixLibrary Amplification Primer MixContains primers complement to P5 & P7 that are required for post-capture PCR and amplifyany library containing P5/P7.4xGen NGS–Made for you
next generation sequencingx G E N A M PLICON PAN E LSxGen Predesigned Amplicon PanelsSARS-CoV-2 Amplicon PanelCatalog #Reactions1000983296ACE2 Gene Amplicon Panel1000983096SARS-CoV-2 S Gene Amplicon Panel1000983196SARS-CoV-2 ARTIC Amplicon Panel v4.110011442500SARS-CoV-2 Midnight Amplicon Panel1000718450056G Oncology & Inherited Disease Amplicon Panel v2100098389657G Pan-Cancer Amplicon Panel1000983796BRCA1/2 Amplicon Panel1000984096BRCA1/2 PALB2 Amplicon Panel1000984096CFTR Amplicon Panel1000984496Colorectal Amplicon Panel1000983496EGFR Pathway Amplicon Panel1000983996Lung Amplicon Panel1000983396Lynch Syndrome Amplicon Panel1000983596Myeloid Amplicon Panel1000983696TP53 Amplicon Panel100098429616S Amplicon Panel v21000982896ITS1 Amplicon Panel1000982996Human Sample ID Amplicon Panel1000984396HS EGFR Pathway Amplicon Panel1000985824Catalog #Reactions1000982796Catalog #Reactions1000984596Catalog #Reactions10009847Plate 1/96 rxn10009848Plate 2/96 rxn10009849Plate 3/96 rxn10009850Plate 4/96 rxn10009846Set 1/4x96 rxn10009851Set 2/4x96 rxn10009852Set 3/4x96 rxn10009853Set 4/4x96 rxnCatalog #Reactions1000985496xGen Amplicon Core KitAmplicon CoreTargeted Amplicon Library Prep Kit for contiguous coverage (overlapping amplicons),comprises multiplexed PCR, indexing PCR, and Normalase reagents (target-specific paneland indexing primers sold separately).xGen Normalase CDI PrimersAmplicon CDI PrimersCDI primers designed for Normalase workflow when using the xGen Amplicon Core Kit.xGen Normalase CDI PrimersAmplicon UDI PrimersPremixed, unique index primers designed for the Normalase workflow when using the xGenAmplicon Core Kit.xGen BufferPEG NaCl BufferBuffer used during the “with bead” purification steps when DNA is eluted into the nextreaction mixture and the beads are reused. Supplied with the xGen Amplicon Core Kit, alsosold separately when additional buffer is needed.xGen NGS—Made for you5
next generation sequencingx G E N NOR MALAS E M ODULExGen Normalase ModuleCatalog #Reactions1000979396Catalog #Reactions1000979496Catalog #Reactions10009796Plate 1/96 rxn10009797Plate 2/96 rxn10009798Plate 3/96 rxn10009799Plate 4/96 rxn10009795Set 1/4x96 rxn10009800Set 2/4x96 rxn10009811Set 3/4x96 rxn10009812Set 4/4x96 rxnNormalase ModuleCore kit for performing Normalase, includes Normalase I & II reagents and terminal primers(indexing primers sold separately).xGen Normalase CDI PrimersCDI PrimersCDI primers designed for the Normalase workflow.xGen Normalase CDI PrimersUDI PrimersPremixed, unique index primers designed for the Normalase workflow. FOR MORE INFORMATION, VISIT WWW.IDTDNA.COM/NGS.For Research Use Only. Not for diagnsotic procedures. Unless otherwise agreed to in writing, IDT does not intend these products to be used in clinical applications and does not warrant theirfitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations. 2022 Integrated DNA Technologies, Inc. All rights reserved. For specific trademark and licensing information, see www.idtdna.com/trademarks. Doc ID: RUO22-0715 001 04/22
xGen Custom Hyb Panels Catalog # Reactions Custom Hyb Panel Provides the best trade-off between speed and value. NGS functional testing documentation included with a 3-week TAT*. Request online 16, 96, & custom Custom Hyb Panel—Accel Has fastest turnaround time for customers ( 5 days)*. Request online 16, 96, & custom Custom Hyb Panel .
