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Fast, accurate sequencingstarts hereAccelerate your NGS success with automated sample prep
Comprehensive, scalable NGS automation solutionsThe most complete and versatile system forautomated NGS sample preparationAgilent offers a complete instrument and reagent solution for automating Next GenerationSequencing (NGS) sample preparation. As a result, you can achieve excellent reliability andreproducibility with easy scale up of your research laboratory throughput using reagents,automation, protocols, and support from a single source.Optimized protocolsOur NGS automation solutions include optimized scheduling protocols for liquidhandling and labware management using VWorks software to easily automateyour NGS sample preparation. Protocols have been fully developed and validatedfor Agilent's SureSelect/HaloPlex library preparation and target enrichmentreagents. Additional automation protocols for select 3rd-party reagent providersare also available.Reproducible dataAutomated liquid handling for library preparation and target enrichment leads tomore consistent yields and higher quality data than manual processing.Higher ThroughputAgilent’s comprehensive and flexible automation solutions reduce manualintervention to increase throughput and maximize your walk-way time.1
Simplify your scale up with modularautomation solutionsAgilent Bravo NGS (NGS A, Option A):High throughput and improved reproducibilitywith a small footprint–– Agilent Bravo Automated Liquid Handling platform–– Agilent 96 Channel Disposable Tip Head–– Agilent Bravo accessories for heating, cooling, shaking,and magnetic separationAgilent Bravo NGS (NGS B, Option B):High throughput and improved reproducibilitywith more walkaway time––––––––Agilent Bravo NGSAgilent BenchCel 4R Microplate HandlerAgilent Labware MiniHubAgilent PlateLoc Thermal Microplate SealerAgilent NGS Workstation delivers higher throughput with less hands-on timeAutomating your NGS sample preparation with either Agilent Bravo NGS or NGS Workstation increaseslaboratory throughput up 3.5 fold compared to manual sample preparation. For whole genome sequencingapplications that require only library preparation, 96 samples can be prepared in less than four hours toincrease laboratory output to 960 sequencer ready libraries in a single week. For more complex protocols, suchas targeted resequencing using Agilent SureSelectXT, both systems can complete library preparation and targetenrichment for 192 samples in a five-day work week.Hands-On Time forcomplete Manual PrepworkflowMaximum Numberof SamplesProcessed/DayHands-On Time forcomplete AutomatedPrep workflowMaximum Numberof SamplesProcessed/DayLibrary Prep375 min8-2425 min96Pre-Capture PCR20 min8-2415 min96Hybridization Preparation20 min8-245 min96Capture210 min8-2420 min96Post-Capture PCR50 min8-2410 min96Totals675 min16-48 per week75 min192 per weekTable 1. NGS Workstation drives 3.5x throughput with 4x less hands-on time by automating pipetting and sample handlingwith Agilent SureSelect XT. Number of samples/day assumes staggering sample processing by end user over multiple days.2
Reproducible genomics protocols made easyThe Bravo Automated Liquid Handling Platform is the core of Agilent NGS automation solutionsHighly Capable Liquid Handler–– Compact design with a small footprint is compatible withmost laboratory bench tops–– Open system for other genomics applications: PCR/qPCRplate setup, CRISPR library preparation, serial dilutions, andnucleic acid purifications–– Fast and flexible processing of full or partial platesReproducible and Accurate Pipetting Performance–– Dispenses from 2 uL to 180 uL with 5 % CV 10 % accuracy–– Installation Qualification (IQ) and Operation Qualification (OQ)services are availableVersatile Platform–– Easily swappable head expands your throughput to 384 wells–– Fully customizable with each position configurable forshaking, heating, cooling, and more–– Easy-to-change pipette heads expand dispensing volumefrom 300 nL to 250 μL in 96, 384, and 1536-well formatsA proven standard for NGS automationBravo liquid handlers deliver reliable performance forNGS at over 150 customer sites worldwide, includingmajor genome research centers, biopharmaceuticalcompanies, and academic research laboratories3
Agilent’s components increase throughput and protectprecious samplesBenchCel 4RMicroplate HandlerPlateLoc ThermalMicroplate SealerLabwareMiniHubThe BenchCel 4R MicroplateHandler is a reliable, rapid roboticplate handler with high-capacitylabware storage. It deliverslabware and reagents between theLabware MiniHub and the Bravoplatform. The BenchCel gives userssubstantially more walkaway timefor liquid-handling intensive NGSsample preparation methods.The programmable PlateLocThermal Microplate Sealer createsa high precision seal that preservesthe integrity of low volume samplesduring heated incubations andeven overnight. It is easy to use andflexible, accommodating a widerange of microplates and tube racks.The Labware MiniHub is a rotating,randomaccess carousel for presentingand storing labware. Featuring userconfigurable spacing to easily hold amix of microplates, reagent reservoirs,and pipette tips, the Labware MiniHubis compact and flexible.Agilent VWorks AutomationControl SoftwareAgilent VWorks is an intuitive schedulingprogram capable of driving theoperation of the Bravo platform andadditional devices. The graphical userinterface makes it easy for users torun existing protocols, develop newprotocols, and monitor progress. Formsare available for SureSelect, HaloPlexand numerous 3rd-party NGS reagentsto save time and maximize productivity.4
Proven protocols streamlinenext generation sequencingsample preparationAutomated sample preparation for NGS sequencing platformsAgilent has developed a complete automation protocol for SureSelectXT librarypreparation and target enrichment reagents for Illumina.SureSelect XT Library PrepShear Genomic DNARepair Ends3’-dA AdditionAdapter LigationPCR EnrichmentSureSelect XT Target EnrichmentAutomatedPurificationSureSelect OligoCapture utomatedBead epped LibraryProcedures automated with Agilent Bravo NGS or NGS Workstation.Procedures not carried out on the Bravo deck.5QCSequencing
Ready to use protocols for automated NGS sample prep18001800160016001400140012001200DNA (ng)DNA (ng)DNA yields of NGS libraries prepared using SureSelectXT on the Bravo platform displayhigher reproducibility as shown by a lower coefficient of variance (CV) compared to 012345678Sample number91011120123456Sample numberIn a comparison of library preparation DNA yield between automated and manual protocols (left and right figures,respectively), the automated samples show a tighter reproducibility of 11 % CV compared to 18 % CV for the manuallyprepared samples.Add higher throughput sample QCThe Agilent 4200 TapeStation system simplifies andexpedites DNA or RNA quality control for NGS applications.Automated process allows for walk-away operation for upto 96 samples. The easy-to-use system delivers results atthe rate of one sample per minute to increase your walkaway time.6
Agilent CrossLab Services:Maximize uptime with end-to-end supportYou can trust Agilent CrossLab service experts to deliver valuable insightsand keep your instruments running at top performance. Our industry-leadingservices—tailored to meet your needs—include technology refresh, applicationconsulting, repairs, preventive maintenance, compliance verification, andeducation. Ask us how we can support your laboratory today.Visit www.agilent.com/crosslabLearn more:www.agilent.com/chem/BravoNGSBuy online:www.agilent.com/chem/storeFind a local Agilent customer center in your country:www.agilent.com/chem/contactusU.S. and Canada1-800-227-9770agilent inquiries@agilent.comEuropeinfo agilent@agilent.comIndialsca-india marketing@agilent.comAsia Pacificinquiry lsca@agilent.comFor Research Use Only. Not for use in diagnostic procedures.This information is subject to change without notice. Agilent Technologies, Inc. 2017Published in the USA, Novermber 16, 20175991-4431EN
handling and labware management using VWorks software to easily automate your NGS sample preparation. Protocols have been fully developed and validated for Agilent's SureSelect/HaloPlex library preparation and target enrichment reagents. Additional automation protocols for select 3rd-party reagent providers are also available. Reproducible data
