Transcription
Driving GPC/SEC ForwardWinGPC UniChrom
About PSSDriving GPC/SEC ForwardPSS GmbH was founded in 1985 by two PhD students at the Physical Chemistry Department, University of Mainz, Germany, producing polymer standards at the University facilities. In the following years PSS expanded staff and productsincluding tailor-made polymers, organic and aqueous GPC/SEC columns, GPC/SEC software and moved 2001 to ownfacilities located in Mainz, Germany. PSS-USA opened its office in 1994, operates and serves North and South Americancustomers from Amherst, Massachusetts. To date, PSS has successfully gained leadership in the overall GPC/SEC market,making innovative contributions not only in Germany and the USA, but around the world.PSS is fully dedicated to the advancement of macromolecular liquid chromatography, by means of materials design,synthesis, manufacturing, consulting, service, and innovative research, applying the highest standard of expertise andreliability. Our close relationship with our customers has helped us to continuously improve the quality of our productsand services. Our high caliber staff, mostly chemists, is experienced, creative and trained in problem solving.Corporations, universities, and organizations in more than 60 countries use our products and profit from our outstandingservice and know-how.Certified DIN ISO EN 9001PSS is certified (DIN ISO EN 9001:2008) to produce high quality reference polymers, GPC/SEC columns and software forthe characterization of polymers by their molecular weight and their structural characteristics. PSS employs the latestfindings in polymer science for the synthesis and characterization of polymers, block copolymersand biopolymers. PSS operates a manufacturing facility equipped with a complete state-ofthe-art characterization laboratory at the headquarters in Mainz, Germany, fully supportingcustomers working under stringent requirements i.e., GLP, DIN, ISO certifications.ContactPSS Polymer Standards Service GmbHIn der Dalheimer Wiese 5D-55120 Mainz, GermanyPhone: 49 6131 96239-0Fax: 49 6131 96239-11info@polymer.dewww.polymer.dePSS-Polymer Standards Service – USA IncAmherst Fields Research Park160 Old Farm Rd, Suite AAmherst, MA 01002 USAPhone: (413) 835 0265Fax:(413) 835 0354pssusa@pssgpcshop.comwww.pssgpcshop.com
Why WinGPC UniChrom?UNIfied Macromolecular Chromatography UNIversal Applicability UNIque Solutions 445Designed for Now and the FutureFrom single instrument to Client/Server ChromPilot System Control Client/Server Solutions 677Full Compliant and ValidatedComplete tracebility, reproducibility and trustworthy results UniChrom Compliance Pack 89Powerful Software Modules for all TasksCopolymer analysis tools Module for 2D Chromatography Module for Chemical Heterogeneity Module for Copolymer Analysis Absolute molar mass and structure tools Modules for Light Scattering Module for Viscometry Module for Mass Spectrometry Module for End Group Analysis 101111111212131313Reporting is a Breeze!WinGPC UniChrom solutions for reporting WinGPC UniChrom Report Designer 1414Supplies and Services for Comprehensive Characterization of Natural and Synthetic MacromoleculesReference Polymer Standards Software Analytical Services GPC/SEC Columns GPC/SEC Instruments GPC/SEC Schools and Support 151515151515Contents
4Why WinGPC UniChrom?RI-Chromatogramm eines oligomeren Phenolformaldehydharzes mit den unterschiedlichenPolymerisationsgraden 1 bis 3 sowie den typischen Systempeaks.Why WinGPCUniChrom?UNIfied Macromolecular ChromatographyUniChrom is a dedicated, modular macromolecular chromatography datasystem (MCDS) for all separation and detection techniques required for thesuccessful analysis of synthetic and natural (bio)polymers, macromoleculesand proteins. No longer will there be a need to learn multiple programs tofully implement macromolecular chromatography within the laboratory.Time and Cost Savings are the result of more efficient allocation ofhuman resources, instruments and increased laboratory productivity:only one environment to learn, validate and maintain.UNIversal ApplicabilityWinGPC UniChrom’s Market-Wide Compatibility lets you acquire data from anycommercial system in the market, allowing you to capitalize on previous instrumentation investment. Convenient instrument control is available for manysystems and vendors. The WinGPC UniChrom permits simultaneous control ofmultiple instruments and detectors.The modular software design fits your changing needs by offering a completepath for expansion from a single instrument to network installations up to trueClient/Server solutions. Seamlessly fitting software modules for new methodscan be added any time making training on new features easy.
5UNIque SolutionsEase of Use:Method, Raw Data,Results plus uncertaintyvalues updated as you go.Be save:The WinGPC UniChromproject manager providesfast information retrievaland highest data security.Get the most outof your data:Combined HPLC andGPC/SEC analysis andmany other options.Ease of UseKeep track:A clear user interface letsyou always see what isgoing on. A bird-eye-viewshows all important parameters andresults simultaneously. All windowsare updated as you go. WinGPCUniChrom lets you monitor multipleinjections, assures complete methoddocumentation and traceability,offers unlimited data acquisition timeand allowes to prioritize samples.AExceptional Data SecurityBe safe:Database driven collectionand retrieval of data providesfast access to sample informationand maximum data security. Allinformation about method, calibration and processing parameters isautomatically stored in the databaseproviding an audit-ready environment with complete documentationin an internal log book. Raw dataare always protected and cannot bedeleted, modified or accidently beoverwritten. The powerful search option ensures fast retrieval of data andall related information.B Convenient Automation& High ThroughputConcentrate on result interpretation while WinGPCproduces results:Wizards and automatic routinesfor calibration, re-calibration, dataevaluation and calculation of productspecific parameters and qualityrelated numbers save mouse clicksand provide results. fast!CIntelligent Solutionsand Powerful ToolsEnhance the productivity without sacrificing theresolution:WinGPC UniChrom’s overlaid injection feature saves about 30 % time(and eluent) required per injectionallowing to inject a sample beforethe last sample run has completed.Achieve the highest throughput byusing WinGPC UniChrom in connection with PSS HighSpeed Columnsand the PSS ReadyCal Standards.DPrecise and accurate results:WinGPC UniChrom analyses automatically the quality of raw andmeta data and calculates uncertaintyvalues for all results and all methods. The detailed description of theparameters contributing to the resultuncertainty helps to identify areas ofimprovement to produce results withhigher quality.Get the most out of your data:Unique algorithms, focused softwaremodules, meaningful overlays anddedicated tools for many applicationsprovide versatility and eliminate theneed for spread sheet calculations.Rely on WinGPC UniChrom for providing traceable and validated results,even for special applications. Askabout our dedicated solutions whichare done on a custom programmingbasis in close cooperation with ourusers!A detailed description of general softwarefeatures, results, supported methods anddetectors can be found on our website:www.polymer.de
6Lab QCWinGPC UniChrom solutions from single workstation to Client/ServerSEC 01PC 1SEC 02Designed for Nowand the FutureSEC 03From single instrument to Client/ServerWinGPC UniChrom is the ideal solution for all laboratories. The flexible designfits your changing needs by offering a complete path for expansion from asingle user/single instrument with only a limited number of detectors to largeinstallations with multiple users and instruments, even up to true Client/Server setups, where all instruments are available any time, anywhere withoutany local installation.Addition of instruments to an existing installation is seamless and easy: Plug-in the instrument(s) Add the license file for more instruments Benefit from running independent systems in a single environment.The new instruments gain the WinGPC UniChrom advantages already experienced, maintaining the existing configurations, data and methods.Seamless software integration alleviates the need for re-installation, re-validation or additional training.
7ChromPilotSystem ControlChromPilotSystem ControlMultiple vendors, multiplesystems, multiple detectorsall controlled by the WinGPCUniChrom ChromPilot.Increase traceability andstreamline your workflowThe ChromPilot adds comprehensiveand convenient system control formany vendors. The future-provenand flexible instrument driverapproach guarantees easy, fast andseamless integration of new systems,when they are launched. Mixedsystems and configurations are fullysupported.Status and settings of the controlled system are displayed in theInstrument Manager, providing allinformation at one glance. TypicalGPC/SEC timed events like turningoff modules, setting parameters(e. g. flow-rate, wavelength) can bedone conveniently in one overviewwindow. All system communicationis logged in an encrypted instrumentaudit trail.Client/ServerSolutionsHighest flexibilityand convenienceThe NetConnect software managesall chromatography systems withinthe department or even companywide via network. Authorized usersmay reserve the requested instruments before starting tests. Whilethe data acquisition is activated, therun can be controlled, modified, orprocessed by any network client withaccess permission rights. The built inaccess control guarantees measurements free of interference from otherusers.UniChrom Client/Server installations improvecompliance and enhance productivity byreduced costs and administration requirements.Contact us to select the ideal setup for your lab.
8ComplianceFull Compliantand ValidatedComplete tracebility, reproducibility and trustworthyresultsThe PSS Quality Management System ensures design, production, sales andsupport following the standard ISO 9001.WinGPC UniChrom assures the quality, integrity and reliability of the completedata system by meeting all validation and compliance requirements in R&Dand QC environments alike. WinGPC UniChrom fully complies with nationaland international GPC standards: ISO/EN 13885, DIN55672, ASTM D 5296-05,JIS, BR etc.WinGPC UniChrom is a commercial off the shelf chromatography program andis a closed system. It is designed to ensure that the installed product is alwaysidentical. The user accessible functionality is controlled by a license key. Thisallows general validation and verification tools that can be directly executedby the user on-site.ValidationPSS WinGPC UniChrom uses additional theoretical molar mass distributions tovalidate software algorithms and data flow path. The documentation of thisvalidation is provided with every license. The validation can be repeated onevery customer workstation to prove operation and correct data flow path onthe workstation.
9UniChromCompliance PackUniChromCompliance Pack21CFR 11 compliance madeeasy and convenient.WinGPC UniChrom is designed inaccor dance with FDA 21CFR11 topro vide a full feature set to helpcompanies using macromolecularchromatography comply with theseregulations.Secure login:Secure login is guaranteed using aunique username and password.A direct link to the Windows authentication ensures that company policyis followed.Administration and User Levels:WinGPC UniChrom offers four different user levels. The Administrationtool allows you to add and removeusers, to assign a level and to setUniChrom administration rights andgroup privileges.The default PSS settings for UniChromprivileges can be adapted by administrators to reflect the tasks in the lab.Electronic Signatures:Results can be electronically signedby users in a two-step process withsigning and reviewing. For signing,username and password identification are required as well as the corresponding WinGPC privilege “Assignelectronic signatures” or „Approveelectronic signatures“.Traceability with Audit TrailsFour different audit trails independently record operator actions: WinGPC sample audit trailWinGPC session audit trailWinGPC system audit trailWinGPC administration audit trailAudit trail entries are time stampedand document user, workstation,action, actual value, if applicableprevious value, and if selected thereason for change. Audit trails areencrypted, only human readablewithin UniChrom and can not beedited or modified.
10Copolymer analysis toolsPowerful SoftwareModules for all TasksCopolymer analysis toolsLearn more about the chemistry of your macromolecular samplesMany samples are copolymers which have a comonomer distribution and amolar mass distribution. The chemical heterogeneity of a product can be animportant parameter for performance and quality.WinGPC UniChrom is the only software that seamlessly integrates any methodand detector. Data can be processed/reprocessed on-line or post-run by freelyselecting the most appropriate data analysis method or method combination.This reveals a wealth of product properties which are never available in anyother software.WinGPC UniChrom offers a variety of data analysis options to meet any R&Dand QA requirement of modern high-tech products. Access the full range ofproduct properties through WinGPC UniChrom’s advanced and unique characterization modules.
11Module for2D ChromatographyThe 2D analysis software modulecombines the separation powerof two different chromatographicmethods (HPLC, SEC/GPC, TREF, IC,CE, GC etc.). This results in a dramatical enhancement of resolution andpeak capacity as well as in-depthinformation on complex products.Data acquisition and control of thefraction transfer between dimensionsis fully automated. Multiple views ofraw and consolidated data, like 3Dsurface plots or contour maps, areavailable. WinGPC UniChrom is theonly software that determines andquantifies multiple property distributions simultaneously.Module forChemicalHeterogeneityThe chemical heterogeneity softwaremodule calculates the chemicalcomposition, the skew and width ofthe compositional distribution basedon HPLC-type separations (PolymerLAC, GPEC). It relies on a compositioncalibration of the chromatographytime base. This calibration can bedone either with standards of knowncomposition or via multiple detection(Copolymer Analysis module).Module forCopolymer Analysis10090SBS 1SBS 2main product:ABA block copolymer80Styrene Content [%]706050styrene content distribution (CCD)40by-product:AB block copolymer2010impurity homo polymer(not present in SBS1)010.511.011.512.012.513.013.514.014.5PSS WinGPC Unity3015.015.516.016.515.015.516.016.5SEC Elution Volume [ml]11.011.512.012.513.013.514.014.5(not present in SBS1)1020impurity homo polymerAB block copolymerby-product:]%[ tnetnoC enerytS3040506070styrene content distribution (CCD)ytinU CPGniW SSPSEC Elution Volume [ml]10.50The copolymer analysis softwaremodule calculates the average chemical composition, the comonomer distribution and the copolymer averagemolecular weights. It uses multipledetectors in GPC/SEC separations.This data processing method needs atleast two independent concentrationsignals for the automatic determination. It relies on the calibration ofthe individual signal responses tomeasure comonomer concentrationin an absolute way.
12Absolute molar mass and structure toolsPowerful SoftwareModules for all tasksAbsolute molar mass and structure toolsDetermine true molar masses and more with advanced detectionThe characterization of modern and highly specialized macromolecules is challenged by the various types of monomers and topologies. The macromoleculararchitecture strongly affects the application properties according to processing ability, rheological behavior and transport properties. GPC/SEC elucidationof these products has become more feasible with the availability of on-linemass spectrometers and molar mass sensitive detectors like light scatteringor viscometer devices. They provide absolute molecular weights, the type ofbranching architecture and the degree of branching.The correlating of light scattering and viscometry information adds reliabilityand substance by combining primary information. The use of viscometry inconjunction with RALLS (Triple Detection) has become standard methodologyto overcome the limitations posed by 90 light scattering only. The combination of the LS module and the viscosity module adds triple evaluation capability. However, WinGPC UniChrom goes one step further by combining MALLSwith viscometry. This allows for the unambiguous absolute measurement ofbranching, aggregation, agglomeration, structure parameters and gel content.Modules forLight ScatteringWinGPC UniChrom offers two lightscattering modules which differ inthe number of supported angles. Forapplications that can be restricted toa single angle the LS module is thesuitable choice for any RALLS or LALLSinstrument. Validated light scatteringdata evaluation yields absolute andreliable measurement of all molarmass averages and their distribution,including dn/dc or recovery determination.The light scattering module formulti angles has all the single anglefeatures as well as the followingadditional information: molecularsize (Rg) and its distribution evaluated with Zimm’s, Debye’s or Berry’smethod, degree of branching andstructure coefficients. WinGPC UniChrom allows monitoring of up to 18different angles and 6 auxiliary analog signals from RI, UV or viscometerat the same time.
13Module forViscometryThe viscosity module supports allviscometers regardless of manufacturer or design principle (e.g. singleor dual capillary, asymmetric or symmetric bridge). Viscometers directlymeasure intrinsic viscosity withoutrelying on assumptions.Validated and proprietary viscometrydata processing methods derive truemolecular weights and reliable MarkHouwink constants based on theuniversal calibration principle. Thebranching index g’ can be measuredacross the molar mass distribution.Module forMass SpectrometryThe mass spectrometry module supports mzXML data import of massspectrometry data to WinGPC UniChrom. The mass spectrum for everyelution volume slice can be reviewedwith automatic assignment of degreeof charge, the degree of polymerization and the molar mass of the endgroup.A dedicated calibration curve can becreated after automatic band broadening correction to generate truemolar masses.Module forEnd Group AnalysisThe end group analysis softwaremodule allows the determination offunctionality distribution and absolute molecular weights without theneed for calibration standards or lightscattering detection. End group dataanalysis only needs two independentdetector signals for the automaticcalculation of desired results.In addition, the calculation of heparinproduct quality in full compliancewith national and international pharmacopoeias (e.g. EP, USP, JP, DAB) canbe done.
14ReportingReportingis a Breeze!WinGPC UniChrom solutions for reportingWinGPC UniChrom lets you report anddistribute information in multiple ways: Export the graphics to word processing toolsTransfer data to LIMS Prepare electronic reports in various formats such as pdf or html Prepare powerful overlays and distribute them to anyone, anyhow,anywhereComplete control of the design and content of your report is achieved from theoptional WinGPC UniChrom Report Designer.InnovativeIdeasThe WinGPC UniChrom Report Designer offers options that outshine anyreport presentation tool. Workflow support on many levels helps to organizeand manage samples, reports and resources: p roduce lab statistics such as degree of utilization for instrumentsor samples/account use automatic custom calculation, e. g. average results for repeated injections select automatic intelligent rounding of results add comments during printout set user selectable QC flags automatically produce powerful overlays with automatic sorting for sample screeningexperiments
15Supplies and Services for Comprehensive Characterizationof Natural and Synthetic MacromoleculesReference Polymer StandardsGPC/SEC Columns GPC/SEC Standards and Kits For all Organic Eluents Certified Reference Materials For all Aqueous Eluents MALDI Kits Viscosity & Light Scattering Validation Kits For High and Low Molecular WeightSynthetic and Bio-Polymers ReadyCal Kits From Micro GPC/SEC up to Preparative Scale Deuterated Polymers HighSpeed Columns for fast Analysis Tailor made Polymers and CopolymersGPC/SEC InstrumentsSoftwareWinGPC UniChrom MacromolecularChromatography Data System Complete Systems and Components Light Scattering Detectors Viscosity Detectors Light Scattering Module for LALLS, RALLS,TALLS, MALLS dn/dc Instrumentation Viscosity ModuleGPC/SEC Schools and Support Copolymer Module End-group Analysis Module 2-dimensional Chromatography Module Heparin Module LAN/Server Solutions Compliance Pack PoroCheck Software for Poresize Analysisand inverse GPC/SECAnalytical Services Molar Mass Determination Branching/Structure Information Method Developement and Transfer Complete Product Deformulation Consulting Full Services from Installation to Validation,Operation, and Repair GPC/SEC and Software Training Schools GPC/SEC In-house Training User Meetings NetCommunity with Application andPublication Downloads
your local distributorwww.polymer.de
Driving GPC/SEC Forward . Driving GPC/SEC Forward PSS GmbH was founded in 1985 by two PhD students at the Physical Chemistry Department, University of Mainz, Ger- . Module for Mass Spectrometry 13 Module for End Group Analysis 13 Reporting is a Breeze! WinGPC UniChrom solutions for reporting 14
