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m o l e c u l a rs p e c t r o s c o p yEvolution 300Evolution 600UV-Vis SpectrophotometersLife ScienceMaterial SciencePharmaceuticalPart of Thermo Fisher Scientific
ContentsOptical Design4System Configuration6Reliable, High-Performance UV-Visible SpectrophotometersLife Science Biochemical 8Pharmaceutical& QC Analysis10Material Science12Smart Accessories14For over 60 years, customers in world-renowned research institutions, highly regulatedQC laboratories and teaching institutions have relied on Thermo Scientific UV-Visible(UV-Vis) spectrophotometers. The Evolution UV-Visible 300/600 spectrophotometer seriesis founded on the successful legacy of the Pye-Unicam, Bausch & Lomb, Philips Scientific,and Spectronic products. Many customers today say they made their first absorbancemeasurement on a SPECTRONIC 20 spectrophotometer, a product on the market since1953. The Evolution 300/600 series continues in this tradition by delivering the solid,high-performance you need for your UV-Vis applications.Thermo Scientific UV-VisSpectrophotometer Product LinesSPECTRONIC 20/20D, SPECTRONIC Educator ,and GENESYS 20 Basic teaching Easy sample and data handlingGENESYS 10 Series Visible, UV-Vis, and UV-Vis scanning options Routine quantitative analysis and common bio assays Easy-to-use accessoriesHelios , Evolution 60, and Evolution 100 Routine quantitative analysis and bio assays More advanced accessories and softwareEvolution 300/600 Series Wide range of accessories Advanced quantitative analysis and bio assays Extensive software offerings Tablet dissolution Material science2The PU8800, designed by Pye Unicam inthe 1980’s, was one of the industry-leadingresearch instruments of its time.
Life ScienceBiochemicalMaterial SciencePharmaceuticalQCThe Evolution 300/600 spectrophotometer seriesincorporates a high-performance optical design, versatileVISION software packages, and the highest qualityaccessories for the most demanding applications. Thesystems offer a multitude of advantages for a widevariety of applications including: Life Science Biochemical Pharmaceutical & QC Material ScienceInnovative Smart Accessories offer ease of use andincreased productivity by providing: Snap-and-go installation Seamless software integration Serial number tracking for the regulated environment System configuration choices offer the ultimate inflexibility for your laboratory. Choose from: Stand-alone Local Control version PC Control version Combination of both3
Evolution 300 & Evolution 600UV-Vis SpectrophotometersHigh-Performance Optical Design for Reliable ResultsMaintaining high performance is the ongoing legacy you can count onwith the Thermo Scientific Evolution 300/600 spectrophotometer series.Evolution 300OpticsOpticsQuartz overcoatedoptics for longlasting reflectivityand cleaning withoutdamaging theoptical surfaceMercury LampPrimary referencestandard, allowsseamless validationand calibration ofthe instrumentDetectorsSilicon photodiodes forextended wavelengthrange to 1100 nm4Light SourceXenon flash lampis only on whentaking measurementsextending its lifetimeSlit MechanismPhoto-etched slitsfor greater accuracy.Stepper-motordriven slit drivewith intelligentoptimizationGrating1200 lines/mm,240 nm blazed,holographic grating,for exceptionalstray lightperformanceChassisRigid, cast-aluminum base with opticalmounts bolted directly onto the base providessuperior system stability and reliable resultsMicrometer MonochromatorDrive with Sliding CouplingProvides excellent accuracy,reproducibility and minimizestemperature effectsThe Evolution 300 optical design includes a long lifetime xenon flash lamp and extended,wavelength-range silicon photodiode detectors. This system is an excellent choice for the routineand research laboratories in the Life Science and Pharmaceutical markets.
Evolution 600OpticsSample CompartmentLarge sample compartmentand wide beam separationwith interchangeablebaseplates for fullsampling hotomultiplier(PMT) detector fordemanding samplesSource OpticsAuto-aligningsource opticsautomaticallyoptimize throughputwhen the lamp ischangedSmart SamplingPlug-in, instrumentcontrolled accessoriesfor ease of useand reliabilityLight SourcesDeuterium andHalogen lamps foradded stabilitywhen measuringminute absorbancechangesMonochromatorBoth models featureresearch grade,Ebert monochromatorfor excellent energythroughputChopperFour-segment chopper runningat 33 Hz eliminates possibleoscillation conflicts with globalmains AC power frequenciesof 60 or 50 HzThe optical design of the Evolution 600 includes dual deuterium and tungsten sources and ahigh-performance photomultiplier detector. These features provide enhanced high-levelperformance for the Material Science market and general research applications.5
Evolution 300 &Evolution 600 UV-VisSpectrophotometersOperational Flexibility to Fit Your Lab NeedsThe Evolution 300/600 series offers the flexibility to choose aconfiguration suited to your needs: Local Control, PC software control,or Local Control with PC software control. Each of these configurationsoffers various levels of operational flexibility to fit the individual ormulti-user laboratory environment.Local ControlPC ControlThe Local Control configuration offerscomplete instrument control, comprehensivemethod and data file manipulation, and savesvaluable bench space. It can also be password protected preventing unwanted accessfrom outside your laboratory. For presentationpurposes, the Local Control screen displaycan be viewed on an external monitor orprojection system using the DVI output.The PC Control configuration allows users tocollect data and distribute it to third-partyapplications for advanced processing andarchival. In multi-user environments, LocalControl with PC software control gives youthe ultimate flexibility.The PC Software Control configurationuses the powerful VISION suite of softwareto control the Evolution 300/600 instruments.This software allows you to collect, store,recall, and analyze your data, using oneconvenient program. From QC quantitativeanalysis measurements to complex enzymaticassays, the right VISION software packageis available for your application.Local and PC ControlThe large, color display and tactile keypadmake the Evolution Local Control systemconvenient and easy-to-use.6The Local Control with PC software controlconfiguration gives you the freedom tochoose the configuration that matches yourexperimental needs. For example, use LocalControl to make a quick absorption measurement to determine the purity of a DNAsample. You do not need to wait for the PC toboot and the software to load. Later, to runa kinetics experiment based on a methodstored on a network drive, simply openVISION software, and switch the instrumentto PC control. This configuration is ideal forlabs with a variety of experimental needs.
VISION Software SuiteVISIONpro is a flexible software packagedesigned for the general research and QClaboratory. When used with VISIONpro, theVISIONlife software package adds the abilityto monitor and calculate reaction kineticsand to perform DNA melting experiments.For those laboratories requiring 21 CFR Part11 compliance, VISIONsecurity sets thestandard for UV-Vis instrumentation.Additional application softwarepackages for the Evolution 300/600 UV-Visseries include: VISION Chroma for colormeasurements and EnzLab for EnzymaticFood Analysis.The standard VISIONpro software offers advanced scanning, multiple fixed wavelength measurements,and multi-wavelength measurements with customized UVcalc. Quantitative analysis and multicomponentanalysis applications are also included.VISION Software Application Comparison ChartSoftwareVISIONproVISIONpro VISIONlifeVISIONsecurityVISIONsecurity VISIONlifeScanningFixed Abs/%TQuantMulticomponent RateDNA Customized ReportingThe Evolution advanced Report Composer inVISIONpro, VISIONlife and VISIONsecuritysoftware allows you to precisely configure theformat of reports. Thesetemplates can be linked toan application methodand used to automaticallyproduce reports in yourcustomized format.Smart StartSmartStart allows each user to set up theSmartStart menu with the applications they usemost often. This increases the throughput of everyuser by eliminating the need to navigate menusand by decreasing the amount of time required totrain users. Load the SmartStart menu with anyapplication, including your stored methods, andwatch the productivity of your lab increase.7
Evolution 300 & Evolution 600UV-Vis SpectrophotometersComprehensive Solutions forLife Science Biochemical ApplicationsThe Evolution 300/600 series is the natural fit for any multi-user, life-scienceresearch laboratory. For the user performing fast or routine assays, such asNucleic Acid tests, to users performing more advanced studies, such asMichaelis-Menton kinetics or Thermal Denaturation/Renaturation assays,these instruments offer a configuration ideal for your laboratory.Nucleic Acid TestsProtein ConcentrationThe Evolution series provides all the necessarytests for determining DNA purity in thepresence of protein or phenol. DNA/RNAconcentrations can be determined by simpleratios or by wavelength scanning. Thewavelength scanning option allows theadded flexibility of checking for potentialcontaminations.Various quantization methods areavailable to determine the concentration ofpurified double-stranded and single-strandedDNA and RNA. Based on user-entered factors,the Evolution instrument measures theabsorbance of the DNA or RNA and calculates the appropriate concentration.The Oligo (Calc Factor) function allowsyou to enter a specific oligonucleotidesequence and from a subsequent absorbancemeasurement, the system calculates the%GC content, MW of the oligo, molar(pmol/ul) concentration of the sample andan estimate of the Tm value.If your research involves the determination ofprotein concentration assays, the EvolutionLocal Control systems provide the standardprotein assays at the push of a button.All of the major, industry-standard proteinconcentration assays at your fingertips.Thermal Denaturation/RenaturationWhether your research examines short orlong DNA or RNA sequences, duplexes ortriplexes, the Evolution VISIONlife ThermalDenaturation/Renaturation system will meetall of your experimental needs. The systemprovides all the tools necessary to controlcomplex heating and cooling profiles withuser defined hold times and ramp ratesdown to 0.1 C/min. Multiple built-in fittingalgorithms are available to calculate the Tmvalue from all types of melting curves.With no external circulating water required, the Evolution spectrophotometerwith the Smart Peltier accessory can reach a maximum temperature of110 C. This extended range provides added flexibility for making accurateTm measurements on very long oligonucleotides.8
KineticsBoth the Local Control and PC Control configurationscater to the user performing single or multi-celldouble-beam kinetics. The Local Control colorscreen makes it easy to distinguish the individualresults from multiple kinetics reactions. Up toseven active reference cuvettes can be used in aseven-sample/seven-reference configuration. Forlaboratories performing more complex assays, suchas inhibitor studies, VISIONlife software providescalculations for the Michaelis-Menton Km and Vmaxparameters using methods such as LineweaverBurke and Eadie-Hoftsee.All of the routine biological assays, including abuilt-in Oligo calculator, are accessible in onlytwo keystrokes.9
Evolution 300 & Evolution 600UV-Vis SpectrophotometersComplete Tools for Pharmaceutical & QC AnalysisQuantitative Analysis Made SimpleThe Evolution Quant application is availablein both the Local Control and PC Controlconfigurations. For very quick and simplequant analysis in a routine QC laboratory,the Local Control configuration with built-in,soft-key menu operation is an excellentchoice. In a regulated environment, theEvolution series provides the tools toachieve 21 CFR Part 11 compliance.The Quant application can use the datafrom up to 50 standards (3 replicates each)to analyze 999 samples (3 replicates each).VISIONpro software provides automatedweight and volume correction eliminatingadditional time-consuming calculations. Ifyour laboratory is performing analysis onmixtures, then the multicomponent analysisapplication, standard with VISIONpro, is ideal.Remote Fiber Optics MeasurementsA remote fiber optic probe allows the user to performabsorption measurements inside almost any vessel. A simplerinse between each measurement makes sample carryovernonexistent. Taking the light to any sample container insteadof wasting time bringing the sample to the instrument in acuvette or test tube, increases sample throughput.10UVcalc for Automated UserCalculationsIf your analyses routinely demand automatedcalculations on experimental data, youwill find the easy-to-use spreadsheetformat of the UVcalc function beneficial.UVcalc allows you to enter and store thecalculation formulas as you develop dataacquisition methods. When the method isrecalled, both the data acquisition and thecalculations are performed automatically,saving valuable time.
Comprehensive Solutions for Regulatory ComplianceThe US FDA Regulation 21 CFR Part 11describes the criteria under which the FDAwill consider electronic records equivalentto paper records and electronic signaturesequivalent to handwritten signatures. Thisregulation applies to all GxP manufacturersand industries that are FDA-regulated.VISIONsecurity software provides allthe tools necessary to help your laboratoryachieve 21 CFR Part 11 compliance.With continually changing requirements to keepyour Evolution spectrophotometer up to regulatorystandards, no supplier in the industry can providea more comprehensive selection of calibrationand verification tools.Whether compliance requires a manuallyinserted, traceable standard or fully automatedintelligent verification, the Thermo Scientificproprietary Smart Calibration ValidationCarousel (CVC) traceable to NIST1 or NPL2standards fully automates testing for: Wavelength accuracy Photometric accuracy Stray light Photometric noise Photometric stability Baseline flatnessTo obtain the best possible accuracy acrossthe entire wavelength range each instrumentis factory calibrated at multiple wavelengthsfrom the ultraviolet to the near-IR region usinga mercury lamp. An optional mercury lampaccessory is available and user installable.VISIONsecurity not only has all of the acquisition,analysis, and reporting capabilities found in theVISIONpro software, but also has the toolsnecessary to control individual or group accessto the instrument, data, and records. Userauthentication at start-up allows event audittrails, electronic signatures, and prevents usewhile operator is away.Tablet DissolutionAn important application where complianceissues are critical is in the testing of soliddosage forms. Thermo Fisher Scientific andSOTAX have entered into a relationship toprovide semi-automated and fully automatedTablet Dissolution testing systems supportingApparatus I, II, IV, V and VI. The systemcombination provides a complete and seamless solution for any dissolution laboratory.By replacing paper-based archival storage withelectronic data storage and data transfer, theproper use of VISIONsecurity software withyour Thermo Scientific spectrophotometer willhelp you pass FDA audits while reducing costsand increasing security.1. National Institute of Standards and Technology, USA2. National Physical Laboratory, UKEvolution 300 with the SOTAX Apparatus IVflow-through system offers a unique solution fora variety of Tablet Dissolution assays11
Evolution 300 & Evolution 600UV-Vis SpectrophotometersHigh-performance Measurements for Material ScienceThe Evolution 300/600 UV-Vis spectrophotometers offer a variety ofaccessories designed to meet your solid sample testing needs. With thehorizontal mounting of our Diffuse Reflectance accessory (DRA) or oursingle-bounce VN Absolute Specular Reflectance accessory, a new world ofmaterial science measurements is available with the Evolution spectrophotometers. Whatever your routine high-performance measurements require,we have a variety of sample handling accessories catering to your needs.VN Absolute Specular ReflectanceAccessoryThe VN accessory offers significant designadvantages over other designs such as relative specular and VW specular reflectanceaccessories. With relative designs, knowncalibrated specular standards, such as afirst-surface aluminum mirror, are required.These designs require finding a suitablestandard and maintaining the quality of thefront surface. VN has an advantage overVW designs because the light beam onlystrikes the sample surface once. The “W”configuration means the sample strikes thesample surface twice. With the innovativeVN accessory, it is easier than ever tomeasure small samples.12Diffuse Reflectance AccessorySolid Sample Holder AccessoryThe Diffuse Reflectance accessory opens thedoor to measuring many samples that werealmost impossible to measure in the past.This accessory offers a horizontal mountingreflectance port to conveniently measureboth delicate and large samples. The doublebeam Spectralon integrating sphere ensuresthe highest possible energy throughput andaccuracy. A transmittance port is included fordiffuse transmittance measurement of solidor turbid liquid materials. The integratingsphere is fully CIE compliant for performingcolor measurements.The Solid Sample Holder provides thecapacity to measure a variety of solidsamples in % Transmission mode. Anassortment of sample holders are availablefor round, square and odd shaped samples.The 2"x 3" sample slides allow otherindustry accessories, such as a relativespecular reflectance accessory, to be usedat various angles of incidence.
The horizontal sample port of the DRAprovides almost unlimited sample sizemeasurements. Diffuse Reflectancesamples using the DRA in combinationwith the VISIONlite ColorCalc softwareprovides routine solutions for CIE Colorand APHA Color compliant measurements.The VISIONlite MaterialsCalc software provides an array ofcalculations ranging from Luminous Transmittance, Haze,UV protection and UPF protection of Fabrics. One of the keycalculations is the luminous transmittance of sunglasses.An example of a pair of high end sunglasses is shown herealong with the luminous transmittance calculation.The VN accessory can easilymeasure sample sizes down to0.5" (12.5 mm) diameter with anangle of incidence of 10 13
Evolution 300 & Evolution 600UV-Vis SpectrophotometersSmart Accessories for Enhanced ProductivityThe Evolution 300/600 UV-Vis series providesSmart Accessories with auto-intelligence. BothLocal Control and PC Control configurationsrecognize individual accessories and automatically identify the accessory’s uniqueserial number. This saves time and enhancesproductivity in regulatory environments.Kinematic mounts allow easy interchangeand reproducible alignment each time theaccessory is removed and installed.VN Absolute SpecularReflectance Accessory14Smart Accessories intelligently interactwith software menus and local control.Simply snap the accessory in place and theappropriate software menu for that accessorywill appear on the screen. All other accessorysoftware menus are hidden eliminatingpossible confusion.Praying Mantis AccessorySmart Thermostatted Rotary 7-Cell ChangerSmart Thermostatted Linear 8-Cell ChangerSmart Peltier ThermostattedSingle Cell HolderCylindrical Cell HolderAdjustable Microcell HolderSmart Calibration Validation Carousel (CVC)
RX2000 RapidMix AccessorynanoCellAccessoryDRA-EV-600 DiffuseReflectance AccessorySolid Sample HolderVERSA Fiber Optic ProbeSmart Sipper Accessory15 , 30 , 45 , and 60 SpecularReflectance AccessoriesTest Tube and Vial HolderLong Pathlength Rectangular Cell HolderTemperature Probe15
Laboratory Solutions Backed by Worldwide Service and SupportTap our expertise throughout the life of your instrument. Thermo Scientific Servicesextends its support throughout our worldwide network of highly trained and certifiedIn addition to these offices, ThermoFisher Scientific maintains a networkof representative organizationsthroughout the world.engineers who are experts in laboratory technologies and applications. Put our teamof experts to work for you in a range of disciplines – from system installation, trainingand technical support, to complete asset management and regulatory complianceAustralia 61 2 8844 9500 analyze.au@thermo.comconsulting. Improve your productivity and lower the cost of instrument ownershipAustria 43 1 333 50340 analyze.at@thermo.comthrough our product support services. Maximize uptime while eliminating theBelgium 32 2 482 30 30 analyze.be@thermo.comuncontrollable cost of unplanned maintenance and repairs. When it’s time toenhance your system, we also offer certified parts and a range of accessories andCanada 1 800 532 4752 analyze.ca@thermo.comconsumables suited to your application.China 86 10 5850 3588 analyze.cn@thermo.comTo learn more about our products and comprehensive service offerings,Denmark 45 70 23 62 60 analyze.dk@thermo.comvisit us at www.thermo.com.France 33 1 60 92 48 00 analyze.fr@thermo.comGermany 49 6103 408 1014 analyze.de@thermo.comIndia 91 22 6742 9434 analyze.in@thermo.comItaly 39 02 950 591 analyze.it@thermo.comJapan 81 45 453 9100 analyze.jp@thermo.comLatin America 1 608 276 5659 analyze.la@thermo.comNetherlands 31 76 587 98 88 analyze.nl@thermo.comSouth Africa 27 11 570 1840 analyze.sa@thermo.comSpain 34 91 657 4930 analyze.es@thermo.comSweden / Norway / Finland 46 8 556 468 00 analyze.se@thermo.comSwitzerland 41 61 48784 00 analyze.ch@thermo.comUK 44 1442 233555 analyze.uk@thermo.comUSA 1 800 532 4752 analyze.us@thermo.comwww.thermo.comThermo Electron Scientific Instruments LLC,Madison, WI USA is ISO Certified. 2007 Thermo Fisher Scientific Inc. All rights reserved.Spectralon is a registered trademark of Labsphere, Inc.All other trademarks are the property of Thermo FisherScientific Inc. and its subsidiaries.Specifications, terms and pricing are subject to change.Not all products are available in all countries. Pleaseconsult your local sales representative for details.BR51226 E 06/07M
stray light performance Evolution 300 Optics The Evolution 300 optical design includes a long lifetime xenon flash lamp and extended, wavelength-range silicon photodiode detectors. This system is an excellent choice for the routine and research lab
