WIXLIB

Solvents for LiquidChromatography・ for HPLC・・・ p.2・ for LC/MS・・・ p.3・ for QTofMS・・・ p.5・ for GPC・・・ p.7・ for JP General Tests・・・ p.8・ GalloTect ・・・ p.9

LC solvents of FUJIFILM Wako Pure Chemical CorporationAs various chromatograph detectors become more improved, the qualitydemanded in the mobile phase (solvent) has become higher and more diversified.We have a lineup of solvents for high-performance liquid chromatograph (HPLC) thatcan be used in LC analysis in general, solvents for high-purity LC/MS, and solventsfor QTofMS, which is suitable for ultrahigh sensitivity MS analysis. We also offeroptimal solvents for use in GPC, and solvents that can be used for JapanesePharmacopoeia tests.List of testing requirement for LC solventsThe proof test items vary even for the same solvent, depending on the detector inwhich it is expected to be used or the application.The solvents under each standard have passed the testing requirement that aresuited to the “expected applications,” in addition to the quality test as radient testFluorescencetestLC/MSAbsorbanceGradient testFluorescencetestParticlesSuitability forLC/MS analysisQTofMSAbsorbanceGradient test*FluorescencetestParticlesSuitability forQTofMS analysisGPCAbsorbanceGradient testSuitability forGPC analysisthe JP GeneralTestsJP methodUSP-NF methodEP method*The gradient test for QTofMS was conducted as a QTofMS-compatible test.Description of testing requirementAbsorbanceGradient testA test to confirm that the fluctuation of the baseline is lower than at the standardlevel when it is used in gradient elution.FluorescencetestA test that compares the quantity of impurities with fluorescence that cause the backgroundnoise or deterioration in detection sensitivity when it is used in fluorescence measurementwith the reference sample by the fluorescence intensity.ParticlesA test to confirm the number of particles that may affect the column life.Suitability forLC/MS analysisA test to confirm that the background noise is suppressed to a low level when it is used inanalysis using a mass spectrometer.Suitability forQTofMS analysisA test to confirm that that baseline fluctuation is lower than the standard level by connectinga column to UHPLC and conducting gradient measurement using QTofMS.Suitability forGPC analysis1A test to confirm that the peak hight that interferes with the analysis is lower thana certain standard level at a specific wavelength.A test to confirm the absorbance after heating with consideration of solvent discolorationcaused by heating during polymer dissolution and analysis.

for High Performance Liquid Chromatography （HPLC）AbsorbanceGradient testFluorescencetestWe offer various solvents of quality appropriate for use in high-performance liquid chromatograph.Product list (Solvents for HPLC)Product 5-06813207-06731209-06791Product NameAcetoneAcetonitrileBenzene1-Butanolt-Butyl Methyl EtherChloroformChloroform, Amylene methylformamideDistilled WaterDichloromethaneEthanol (99.5)Ethyl aneMethanol1-Propanol2-PropanolTetrahydrofuran, with StabilizerTetrahydrofuran, Stabilizer Free2,2,4-TrimethylpentaneToluenePackage 1L3L100 mL500 mL1 L3L1L1L3L1L1L3 L1L3L1L3L1L1L2

for LC/MSAbsorbanceGradient testFluorescencetestParticlesSuitability forLC/MS analysisThese are dedicated solvents for LC/MS for which LC/MS compatibility has been confirmed.They are also recommended for ultrahigh-performance liquid chromatograph (UHPLC), as theyare guaranteed for particles.A glass bottle with special treatment to suppress the elution of impurities is used as thecontainer*.*Patent No. WO/2005/047884Point for high recommendation 1The cap material for LC/MS solvents was changed from aluminum to a special resin.While the conventional aluminum caps posed a risk of aluminum dust adhering to the bottlemouth and contaminating the solvent, the new resin cap has no dust generation orcomponent elution from the material. It maintains the high-quality state of the solvent.Point for high recommendation 2The quality was improved further by a review on the manufacturing method.The baseline is suppressed at an even lower level compared to the conventional products.Comparison of baselines for acetonitrile (comparison of our products)Before improvement After improvement 3

Example of product specifications 0 ）[g/mL]Refractive index nD２０Water [％]Residue after evaporation [％]Acidity（as CH3COOH） [％]Ammonium（NH4） [ppm]Peroxides（as H2O2）[ppm]Substances reducing permanganate (as O)Gradient testParticles（0.5μm or more)[ number of particles per mL ]Absorbance200nm210nm220nm230nm240nmFluorescence testSuitability for LC/MS analysisSPECIFICATIONmin. 99.90.780 0.7831.343 1.346max. 0.03max. 0.001max. 0.001max. 0.3max. 5to pass testto pass testmax. 100max. 0.04以下max. 0.03以下max. 0.02以下max. 0.01以下max. 0.005以下to pass testto pass testProduct list (Solvents for LC/MS)Product 01301210-01303Product NameAcetonitrileEthanol (99.5)0.1vol% Formic Acid-AcetonitrilePackage Size1L3L1L3L1L3L131313Methanol2-PropanolUltrapure WaterLLLLLLProduct list ( Mobile phase additives for LC/MS)Product Number014-20063018-20061063-04533067-04531Product NameAcetic AcidFormic Acid (abt. 99%)Package Size1 mL 5A50 mL1 mL 5A50 mL4

for high-resolution MS (for QTofMS)AbsorbanceGradient test*FluorescencetestParticlesSuitability forQTofMS analysis*The gradient test for QTofMS was conducted as a QTofMS-compatible test.Since a high-performance liquid chromatograph-massspectrometer (LC-MS) has excellent characteristics, including thewidth of the scope of measuring objects and selectivity, it hasbecome popular in a wide range of fields. Mass spectrometerswith higher sensitivity and resolution have become popularizedthese days, and they are applied in research of metabolomics,proteomics, etc.This product is a high-purity solvent optimal for measurementsusing high-sensitivity and high-resolution MS Testing requirement for compatibility with QTofMS A wide mass range by full scan is guaranteed. (mass range: 50–3000) Quality assurance with utilization of multivariate analysis-principalcomponent analysis. Gradient measurement by column connection (UHPLC measurement) Particle assurance Content and container that ensure that the solvent can be used inhigh-purity conditions at any time 500 mL or 1 L content optimal for using up Glass bottles with special treatment that suppresses the elution of impuritiesare used (Patent No. WO/2005/047884)Low background noiseCompany ACompany Bfor QTofMSComparison of background noise between our solvent for QTofMS andLC/MS solvents of other companies (Companies A and B)5We reviewed the manufacturing processesfrom various points so that it can be used inhigh-performance MS.This product showed a lower backgroundnoise compared to the LC/MS solvents ofother companies (Companies A and B) inquality confirmation using QTofMS.[Total ion chromatogram]Green: Company APurple: Company BRed: for QTofMS (Fujifilm Wako)

Small packaging for usabilityOnce opened, solvents become contaminated due to entry of air in laboratory, etc.To prevent contamination after opening, this product comes in 500 mL or 1 L contentthat can be used up.These are the chromatograms immediatelyafter opening (green) and 1 week afteropening (red). Since it is highly likely thatthe increase in background noise would causeadverse effects on the measurement results inhigh-performance mass spectrometers, it isrecommended that the mobile phase is usedup each time.1 week laterImmediatelyafter openingProduct list (for QTofMS)Product Number018-26225016-26221130-18545138-18541Product 01Ultrapure WaterPackage Size500 mL１L500 mL1L500 mL1LConditions and equipment for data acquisitionConditions for data acquisitionHPLCACQUITY UPLC H-Class (Waters)ColumnWakopak Ultra C18-2, 2.1mmφ 50mmColumn temperature 40 0.3 EluateAcetonitrile : Water [Gradient]DetectorUV 210nmMass SpectrometerTime-of-Flight Mass Spectrometer (Waters Xevo G2-XS)IonizationESIMass Rangem/z 50 3000PolaritypositiveWaters Xevo G2-XS6

for GPCGradient testAbsorbanceSuitability forGPC analysisGPC solvents are solvents for which it has been confirmed that there is no coloring underheating for polymer dissolution or analysis. They are also guaranteed moisture, peroxides,non-volatile substances or the less changes in refractive index or ultraviolet absorptioncaused by impurities. This grades are optimal for preparation of GPC eluents.Example of product specifications IFICATIONAppearanceColorless clear liquidDensity (20 ) (g/mL)Absorbance1.617-1.624200 nmmax. 0.05220 nmmax. 0.03240 nmmax. 0.02254-400 nmmax. 0.01Suitability for GPC testto pass testWater (%)max. 0.03Residue after evaporation (%)max. 0.001Acidity (as HF) (%)max. 0.001Peroxides (as H2O2) (ppm)max. 5Assay (cGC) (%)min. 99.5Product list (for GPC)Product 201-20073209-20091205-20093７Product NameChloroformChloroform, Amylene ethylformamideDimethyl -2-pyrrolidoneTetrahydrofuran, Stabilizer FreeTetrahydrofuran, with StabilizerPackage Size1L3L3L1L3L1L3L1L3L1L3L100 mL500 mL1L3L1L3L1L3L

for the Japanese Pharmacopoeia General TestsJP methodUSP-NF methodEP methodThe solvents for the Japanese Pharmacopoeia general tests (liquid chromatography) are HPLCsolvents for which compliance with the Japanese Pharmacopoeia (JP) reagent standards, theUnited States Pharmacopoeia (USP) reagent standards, and the European Pharmacopeia (EP)reagent standards is guaranteed, and for which some testing requirement of our company’sown have been added.Example of product specifications (Acetonitrile)Example of product labelREQUIREMENTDescriptionIndicates the expiration dateJP method（*added requirementby us)USP-NF methodEP methodColorless clear liquid Colorless clear liquid Colorless clear liquidColor (APHA)-max. 10-Density0.780 - 0.783 g/ml*-Sp. Gr. (20/20 )about 0.78Refractive ｎD201.343 1.346*-about 1.344Absorbance---190 nm-max. 1.00-200 nmmax. 0.07 (0.05*）-max. 0.10210 nmmax. 0.046 (0.03*）--Indicates theguaranteed items*220 nmmax. 0.027 (0.02 ）max. 0.05-230 nmmax. 0.014 (0.01*）----240 nm max. 0.009 (0.005*）254 nm-max. 0.01250-280 nm-max. 0.01-255-420 nm--max. 0.01--max. 0.01Gradient test240-800 nmto pass test*to pass test-Residue afterevaporation0.001 ％*0.005%以下-*Titrable acidAcidity 0.001 ％8 μeq/g以下Titrable base-0.6 μeq/g以下-Watermax. 0.05 ％*0.003-pH (100g/L)--Neutral to litmuspaperDistillation range(80-82 )--min. 95 %Ammonium (NH4)0.3 ppm*------*Peroxides (H2O2)5 ppmSubstances reducingpermanganateto pass test**Fluorescence testto pass testAssaymin. 99.8 ％*--min. 99.5 ％min. 99.9 %Product list (Solvents for the Japanese Pharmacopoeia General Tests)Product NumberProduct 13L3 L 2136-15661132-15663Package Size3L3 L 2Tetrahydrofuran, Stabilizer Free1L8