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OverviewQuantiNova SYBR Green PCR KitUltrafast, highly sensitive, SYBR Green real-time PCRProcedureStringenthot startSingle copydetectionWide dynamicrangeSuperiorprecisionFor all cyclersOrderinginformationSample to Insight
QuantiNova SYBR Green PCR KitOverviewUltrafast procedure with built-in pipetting controlRNA templateProcedureStringenthot startQuantiNova ReverseTranscription Kit20 minDNA templateHeat activation2 minSingle copydetectionWide dynamicrangeSuperiorprecisionFor all cyclersOrderinginformationSample to InsightDenaturation5s35–40 cyclesCombined annealing/extension10 sFastest results Fastest cycling conditions Simple, color change verifies correct pipettingDownloadQuick-StartProtocol
QuantiNova SYBR Green PCR KitOverviewTrue, room-temperature setup with antibodymediated hot startProcedureStringenthot startSingle copydetectionWide dynamicrangeSuperiorprecisionInactive QuantiNovaDNA PolymeraseActive QuantiNovaDNA PolymeraseHeat activation2 min, 95 CQuantiNova DNAPolymeraseQuantiNova AntibodyQuantiNova GuardDNAFor all cyclersOrderinginformationSample to Insight O ur stringent hot start prevents primer dimer formation andextension of non-specific products
4540QuantiNova SYBR Green PCR Kit353025OverviewSensitive detection – even single target molecules0.1130 ng10100Copy number (CFTR)3 ng0.3 ng10000.03 ng100003 pg DNAProcedureStringenthot startCq valueDetection frequency (%)4580406035Single copydetection403020250.1Wide dynamicrange130 ng10100Copy number (CFTR)3 ng0.3 ng100010000001–4Copy number (CFTR)0.03 ng3 pg DNACalculated (Poisson's equation)ExperimentalSuperiorprecisionDetection frequency (%)T he calculated and experimental detection frequencies were highly concordant, demonstrating high80sensitivity and robustnessFor all cyclers6040OrderinginformationSample to Insight 20Robust detection of rare targets001–4Copy number (CFTR)Calculated (Poisson's equation)Experimental
OverviewHeLa cDNAViiA 7CFX96100 ng11.3311.5510 ng14.6714.941 ng18.1118.37100 pg21.7422.1210 pg25.0825.40PCR efficiency95%7QuantiNova AppliedSYBRBiosystemsGreenViiAPCRKit Rn4.03.0Accurate quantitation over a wide dynamic range1 pg28.5728.74100 fg31.8532.2910 fg35.3936.02––95%94%No template controlPCR efficiency2.01.010 fg01Procedure510Applied Biosystems ViiA 7Stringenthot startSingle copydetectionWidedynamicrangeSuperiorprecisionHeLa cDNAViiA 7CFX96100 ng11.3311.5510 ng14.6714.941 ng18.1118.37100 pg21.7422.1210 pg25.0825.401 pg28.5728.74100 fg31.8532.2910 fg35.3936.02––95%94%No template controlPCR efficiency RnRFU4.020003.015002.010001.050010 1520Cycle253035401.0 Accurate quantitation over an 8 log dynamic range10 fg1Sample to Insight10 fg0510152025CycleBio-Rad CFX9630354030354010 fg005101520CycleTen-fold dilutions of HeLa cDNA were assayed for beta-actin.200002025CycleBio-Rad CFX96 RnOrderinginformation15Bio-Rad CFX960Applied Biosystems ViiA 7For all cyclers94%25303540
40353025OverviewQIAGENSupplier BQuantiNova SYBR GreenPCRKitAmplificationefficiency: 83%Amplificationefficiency: 97%20Superior assay precision150.010.11cDNA template (ng)10ProcedureCq value40Stringenthot start30Amplificationefficiency: 83%Amplificationefficiency: 97%25Wide dynamicrange40302520150.01150.010.11cDNA template (ng)10QIAGENSupplier L3520SuperiorprecisionAmplificationefficiency: 102%0.11cDNA template (ng)Ten-fold dilutions of plasmid DNA were assayed using QuantiTect Primer assays for EGFR.Cq valueFor all cyclers4035Sample to InsightQIAGENSupplier B35Single copydetectionOrderinginformationCq value 30252015QIAGENSupplier LAmplificationefficiency: 81%Amplification Higherreproducibility and reaction efficiencyefficiency: 102%Amplificationefficiency: 81%10
10.00.68.00.66.00.30510152025Cycle3035RFU Norm. Fluoro.60000.60.30005Procedure1QIAGEN Rotor-Gene QQIAGEN Rotor-Gene QNorm. Fluoro.0.9 Norm. Fluoro.Stringenthot start0.90.60.60.30.30Single copydetection10 00 02025Cycle3035040 Rn3035303540Bio-Rad CFX96 3540Applied Biosystems 7900HT Fast (384-well)Sample to 025Cycle15 1520 2.0 4.0 E-1500000 05 0 10 5 1510 2015 2520 3025Cycle Cycle4005101520 5 3540 4015 2520 3025 30Cycle Cycle10151520 2025 2530 3035 3540CycleCycle4.03.00 01 5 50 10 10 15 15 20 2025 2530 3035 3540CycleCycle10 2015 2520 3025 3530050 105 15Cycle Cycle40403540Applied Biosystems StepOnePlus BiosystemsViiA 7AppliedAppliedBiosystemsStepOnePlus Bio-Rad CFX96Applied Biosystems7900HTFast (384-well) Rn302535304035402520 3025 3530Cycle40357.0 Rn Rn6.0 7.0 7.55.0 6.06.04.0 5.03.0 4.0 4.52.0 3.0 3.03530403540303540409.0 E-14.0 E-15004.00 E-10 0 0 5050 Rn7.5 Rn Rn7.06.0 7.56.04.5 6.05.0101520 25 30Cycle1520 2025 25301015Cycle Cycle3540353040351.0 2.00 1.00 Rn7.06.04.03530403540303540Applied Biosystems StepOnePlus Applied Biosystems 7500 Fast5.03.02.01.00404035Applied Biosystems ViiA 7Applied Biosystems 7500 Fast3.02.01.01 054011015510202530Cycle152025Cycle35403035Applied Biosystems 7500 Fast Rn17.5 Rn4.02.03.02.01.01.51.001.55 0 10152025303540Cycle1015 1520 2025 2530 3035 3540Cycle Cycle00 1510151510152025Cycle2025Cycle3035403035401510 Reliable and consistent results with various real-time PCR cyclers1050401Applied Biosystems ViiA 7AppliedAppliedBiosystemsStepOnePlusBiosystemsViiA 751510Applied Biosystems 7500 FastApplied Biosystems 7500 Fast Rn4.0 Rn3.02.04.03.040 Rn4.000 1 5 5 10 1015 1520 2025 2530Cycle Cycle1510152025Cycle4.011.07.0 Rn02.0 Rn 2.4 Rn6.05.0 1.42.04.54.09.0 E-11.0 3.0 3.04.0 E-12.00 1.0 01.51 0 5 025 3530 40350 10 5 15 10 2015 2520 30Cycle Cycle4015 1 10152025303551015202530Cycle Cycle1.03.0 Rn6.02.0Applied Biosystems StepOnePlus Rn AppliedBiosystemsBiosystems7500 FastApplied7900HTFast (384-well) RnApplied AppliedBiosystemsStepOnePlusBiosystemsViiA 71.97.55.0Bio-Rad BiosystemsCFX96 ViiA 7Applied500 3.01.52.00 1.009.0 E-15000 4.0 E-1011510 2015 252005 0 1050Cycle4005 0 105 1510Cycle2015Cycle Bio-RadBiosystemsCFX96 ViiA 7Applied Applied Biosystems7900HTFast (384-well) RnBio-Rad CFX962.41.4100030001.04.5150010001.43.010005001.5 9.0 E-15000 0 4.0 E-120001.9 2.4Orderinginformation305153.06.09.0 E-110004.00 2.000 0 1 50 5 10 1015 1520 2025 2530 3035Cycle Cycle05 0 1015510 2015 2520 3025Cycle Cycle0 RnExpression of EGFR in HeLa cells was analyzed using a 06.03.0Dilutionsof the resulting cDNA from 10 ng to 0.01 ng wereamplified on 8 different cyclers.1.94.515003.01.4For all cyclers25101.99.0 E-1 1.410001.9406.07.06.015006.04.51000 5.03.04.040RFU 2.4 Rn0002.4354.02.4Applied Biosystems 7900HT Fast (384-well)Agilent Mx3005P Rn301.48.0 14.0 RFU 2.412.0 20001.910.012.0 14.0 90001.910.0 12.08.0 10.01.460006.0 8.09.0 E-14.0 6.03000E-12.0 4.04.00 2.0 014.0 9000Wide dynamicrange1010Fluorescence Rn (483–533)2000 Rn7.59550 RocheLightCycler480 Fast (384-well)AppliedBiosystems7900HT9000 RnRFU2000RFU7.5 Rn0 Bio-RadCFX96QIAGENRotor-GeneQ Roche LightCycler480 Agilent ce(dR)03514.0 Fluorescence2.4Fluorescence(dR)(483–533)orm. Fluoro.000300 0 RnRFU Rn0002025Cycle40Applied BiosystemsAgilentStepOnePlusMx3005P 00HTFast (384-well) Fluorescence (dR)Roche AgilentLightCycler480 Mx3005PQIAGEN Rotor-Gene Quorescence (dR)35 RocheLightCycler480Fast (384-well)AppliedBiosystems7900HT Fluorescence(483–533) RnAgilent Mx3005PRoche LightCycler480 Agilent Mx3005P 153014.0 9000150012.00.610.0100060008.00.3500 6.04.0 300030001052025Cycle1015Consistently high performance regardless of cycler,cycling conditions or presence of ROXOverview5155004.002.02000 Fluorescence0.9 Fluorescence(483–533)(dR)0.915 0 1019000 Norm. Fluoro.0040Agilent Mx3005P QIAGEN Rotor-Gene QFluorescence (dR)3QuantiNova SYBR Green PCR Cycle303540401
QuantiNova SYBR Green PCR KitOrdering InformationOverviewProcedureStringenthot startProductDescriptionCat. no.QuantiNova SYBR GreenPCR Kit (100)For 100 x 25 µl reactions: 1 ml 2x QuantiNova Probe PCRMaster Mix, 500 µl QuantiNova Yellow Template Dilution Buffer,250 µl QN ROX Reference Dye, 1.9 ml RNase-Free Water208252QuantiNova SYBR GreenPCR Kit (500)For 500 x 20 µl reactions: 3 x 1.7 ml 2x QuantiNova SYBR GreenPCR Master Mix, 500 µl QuantiNova Yellow Template Dilution Buffer,1 ml QN ROX Reference Dye, 1.9 ml Water208254QuantiNova SYBR GreenPCR Kit (2500)For 2500 x 25 µl reactions: 15 x 1.7 ml 2x Master Mix (containsROX dye), 5 x 500 µl QuantiNova Yellow Template Dilution Buffer,5 x 1 ml QN ROX Reference Dye, 5 x 1.9 ml RNase-Free Water208256Single copydetectionWide dynamicrangeSuperiorprecisionFor all cyclersOrderinginformationFor up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual.QIAGEN kit handbooks and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Servicesor your local distributor.Trademarks: QIAGEN , Sample to Insight , QuantiNova , Rotor-Gene , QuantiTect (QIAGEN Group); LightCycler (Roche );StepOne , ViiA , 7900HT Fast , ViiA (Applied Biosystems ); CFX96 (BioRad ); Mx3005P (Agilent ). 2018 QIAGEN, all rights reserved.Ordering www.qiagen.com/contact Technical Support support.qiagen.com Website www.qiagen.comSample to InsightVisit Webpage
QuantiNova SYBR Green PCR Kit Ultrafast, highly sensitive, SYBR Green real-time PCR For all cyclers Ultrafast procedure with built-in pipetting control Fastest results Download Quick-Start Protocol Fastest cycling conditions Simple, color change verifies correct pipetting True, room-temperature setup with antibody-
