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Cytek DxP AthenaChoose from configurations with up to 3 lasers and 13 fluorescent detectors.
Cytek DxP Athena Start accelerating your research.CytekBiosciencesFrom multicolor systems withtechnology to resolve dim populationsto comprehensive service planofferings, Cytek has the solutionthat aligns with your needs.2Cytek Biosciences, Inc.46107 Landing Parkway, Fremont, CA 94538(877) 92-CYTEKsales@cytekbio.com
Cytek DxP Athena Start accelerating your research.Meet TheDxP Athena Built by Cytek from the ground up.The DxP Athena flow cytometry system incorporates Cytek’s provenDxP technology enabling the capability to resolve dim populations in amulticolor format. Multiple configurations available from 2 lasers and 6colors up to 3 lasers and 13 colors. Each configuration offers consistentpowerful performance to meet your application requirements at anaffordable price.13 ColorConfigurationBlue Laser45mWRed Laser80mWViolet Laser50mWCytek Biosciences, Inc.LaserFilterFSC488/10SSC488/10Channel 1530/30FITC, Alexa Fluor 488Channel 2575/30PEChannel 3615/25PE-Texas Red , PE/Dazzle 594Channel 4695/40PerCP-Cy5.5Channel 5780/60PE-Cy7Channel 1661/16APCChannel 2710/50Alexa Fluor 700, APC-Cy5.5Channel 3780/60APC-Cy7, APC-H7Channel 1450/50Brilliant Violet 421, Pacific Blue Channel 2525/50Brilliant Violet 510Channel 3615/25Brilliant Violet 605, eFluor 605NCChannel 4710/50Brilliant Violet 711Channel 5780/60Brilliant Violet 78546107 Landing Parkway, Fremont, CA 94538(877) 92-CYTEKCommon Dyes (not exclusive)sales@cytekbio.com3
Cytek DxP Athena Start accelerating your research.FlexibilityEasily change filters to meet yourspecific application requirements.4Cytek Biosciences, Inc.46107 Landing Parkway, Fremont, CA 94538(877) 92-CYTEKsales@cytekbio.com
Cytek DxP Athena Upgrade from 6-color to 13-colorDxP Athena ConfigurationsStart accelerating your research.Cytek Biosciences, Inc.46107 Landing Parkway, Fremont, CA 94538(877) 92-CYTEKsales@cytekbio.com5
Cytek DxP Athena BV510BV421FITCAPCPEAPC-Cy7PE Tx-Red 8 Peak BeadsPerCP-Cy5.5PE-Cy7Start accelerating your research.6Cytek Biosciences, Inc.46107 Landing Parkway, Fremont, CA 94538(877) 92-CYTEKsales@cytekbio.com
(877) 92-CYTEKCD56 PESSCCD19 BV605B and NK CellsNonT-cellsCD8 PerCP-Cy5.5NK Cell SubsetsCD3 FITCCD8 PerCP-Cy5.5CD27 BV785CD27 BV785CCR7 BV421CD8 memory/effectorCD4sCD27 BV785CD27 BV785CCR7 BV421CD4 memory/effectorCD4 APC-Cy7CD45RA BV510CD45RA BV510T-cellsCD45RA BV510CD45RA BV510CD127 Alexa 64746107 Landing Parkway, Fremont, CA 94538CD127 Alexa 647Cytek Biosciences, Inc.CD56 PECD8sCD45RA BV510CD25 PE-Cy7Regulatory T-cellsCD56 PENK T-cellsCD127 Alexa 64710 Color AssayCytek DxP Athena Start accelerating your research.sales@cytekbio.com7CD8 PerCP-Cy5.5
Cytek DxP Athena Start accelerating your research.RESOLUTION OF DIM POPULATIONSBenefitsIncorporates DxP Technology with efficientphotomultiplier tubes (PMT) enabling high sensitivityand resolution.Cytek provides compactand affordable instrumentswith multiplexing capabilitythat streamline workflowsBUILT-IN MAINTENANCE CAPABILITYAutomated monthly clean bleach cycle minimizesdowntime, streamlines maintenance, and encouragesand deliver a high level ofcompliance.biological clarity for amajority of applications.FUTURE-PROOFINGUpgradeable to 3 lasers and 13 colors. An optional 96Well Automated Micro-Sampler(AMS) is available.SMALL FOOTPRINT55 x 52.4 x 57.8 cm(21.7 x 20.6 x 22.8 in)REPRODUCIBLE RESULTSQbSure software characterizes the detectors andensures optimal daily instrument performance.REFERENCESDxP Athena incorporates the same DxP Technologyused in other Cytek upgraded platforms that arereferenced in over 100 publications.8Cytek Biosciences, Inc.46107 Landing Parkway, Fremont, CA 94538(877) 92-CYTEKsales@cytekbio.com
Cytek DxP Athena Start accelerating your research.Cytek’s ExclusiveFlowJo Collector’s EditionSoftwareDriving the powerful DxP technologyis Cytek’s Exclusive FlowJo Collector’sEdition Software. With this intuitivesoftware you enjoy high-speed dataacquisition along with the tools requiredto optimize setup for a wide range ofapplications.Learn more about FlowJo Collector’sEdition software at www.cytekbio.comCytek Biosciences, Inc.46107 Landing Parkway, Fremont, CA 94538(877) 92-CYTEKsales@cytekbio.com9
Cytek DxP Athena Start accelerating your research.QbSure SoftwareTMEnsures optimal daily performance.By using Cytek’s validation beads and our innovative QbSure performance trackingsoftware, you can feel confident that your cytometer is performing consistently.10Cytek Biosciences, Inc.46107 Landing Parkway, Fremont, CA 94538(877) 92-CYTEKsales@cytekbio.com
Cytek DxP Athena Start accelerating your research.Options96-Well Auto-samplingCytek’s automatic micro-sampler canbe mounted directly under the sampleinjection tube reducing dead volume andincreasing throughput.Auto-modeFull 96-well plate in 40 minutes (15 sec.acquisition/well and 10 sec. wash/well) lessthan 1% carryover.High throughput modeFull 96-well plate in 15 minutes. (9 sec.acquisition/well) less than 3% carryover.Cytek Biosciences, Inc.46107 Landing Parkway, Fremont, CA 94538(877) 92-CYTEKsales@cytekbio.com11
Cytek DxP Athena Start accelerating your research.DxP Athena Technical SpecificationsExcitation OpticsEmission OpticsFluidicsOptical PlatformOptical CouplingSample Flow RatesAllows up to 3 lasers. Fixed optical assemblywith three spatially separated laser beams.Fused silica cuvette coupled to emissionlens by refractive index matching optical gelfor optimum collection efficiency.Front panel keypad provides four modes:Lasers407nm: 50mW488nm: 45mWRun, Standby, Prime and CleanThree preset flow rates:Background RejectionObscuration blade and slit for minimizingLo: 12 μL/minMed: 35 μL/min637nm: 80mWunwanted laser radiation at the detector.Hi: 60 μL/minBeam GeometryForward Scatter DetectorStandard Fluidic ReservoirsPrismatic expander and achromaticspherical lens.And FilterOne 4-L sheath container and one 4-L wastecontainer provided.Optical EfficiencyPower loss at flow cell: 30% of specified laser power.High-performance solid-state silicondetector with 488nm bandpass filter.Side Scatter DetectorPhotomultiplier and 488nm bandpass filter.Photomultiplier TubesHigh efficiency Tri-alkali PhotomultipliersFluorescence Detectors AndFiltersDxP Athena 10-color configuration shown.BluFL1: 530/30 (FITC)BluFL2: 575/30 (PE)BluFL3: 695/40 (PerCP-Cy5.5)BluFL4: 780/60 (PE-Cy7)RedFL1: 661/16 (APC)RedFL2: 780/60 (APC-Cy7)VioFL1: 450/50 (Brilliant Violet 421)VioFL2: 525/50 (Brilliant Violet 510)VioFL3: 615/25 (Brilliant Violet 605)VioFL4: 780/60 (Brilliant Violet 785)12Cytek Biosciences, Inc.46107 Landing Parkway, Fremont, CA 94538(877) 92-CYTEKsales@cytekbio.com
Cytek DxP Athena Start accelerating your research.DxP Technology PerformanceFluorescence SensitivityMolecules of Equivalent Fluorescence (MEFL),using Q&b Method*Min. QMax. b**Typical R 75001161BV4210.013750818BV5100.0115001261*Q measures optical efficiency, b measures background, and R (resolution limit), measuresthe number of dye molecules required to resolve a dim population from noise.**Average R value across 4 systems. MEFL required to be 2 standard deviations abovenoise. Assumes no compensation applied.Fluorescence SensitivityThresholdForward and Side ScatterSensitivityFluorescence ResolutionFITC: 50 molecules of equivalent solublefluorochrome (MEFLFITC).Enables separation of fixed platelets fromnoise.Data Acquisition RatePE: 30 molecules of equivalent solublefluorochrome (MEFL-PE).Forward and Side ScatterResolutionFITC and PE measurements performedusing SPHERO Rainbow Calibration Particle(RCP-30-5A).18 bit 5 log decades.7,500 events/s with beads.Performance is optimized for resolvinglymphocytes, monocytes, and granulocytes.Side Scatter ResolutionFluorescence LinearityDelivers doublet/singlet ratio of 1.95–2.05for CEN stained with Propidium IodideCapable to resolve 0.5μm beads from thenoise.excited with the 488nm laser.Cytek Biosciences, Inc.46107 Landing Parkway, Fremont, CA 94538(877) 92-CYTEKsales@cytekbio.com13
Cytek DxP Athena Start accelerating your research.Data ManagementFlowJo Collector’s Edition 7.5.110 or laterOur acquisition interface can be adapted to any application. Real time spillover matrix for viewing livecompensated data. Acquisition templates include hardware, gate, spillover, layout and statistical settings.Save workspaces and use during multiple acquisition sessions.Cytek AMS Software Version 1.0.4Fast and Easy SetupExperiment plate mapping, including stain names can be setup on stand alone computer and saved as atemplate for future use.Well ID StampingWell ID in the file name confirms data file to Well ID relationship.Block InspectorChange the FlowJo Collector’s Edition acquisition settings on a per block basis.14Cytek Biosciences, Inc.46107 Landing Parkway, Fremont, CA 94538(877) 92-CYTEKsales@cytekbio.com
Cytek DxP Athena Start accelerating your research.WorkstationOperating SystemWindows 7 32-Bit ProfessionalProcessorIntel Quad Core processor, 3.0 GHzRAM4GB (1 x 4GB), 16000 MHz DDR3Hard Drive500GB SATA 3.0Gb/sDVD Drive16x DVD /- RW, SATAVideo ProcessorHD Graphics GMA sDimensions (W x D x H)PowerSensor module (withoutAMS)100-240V, 50/60 Hz, 2A max55 x 52.4 x 57.8 cm450 watts with all solid-state lasersWeightTemperature45.4 kg (100 lbs)16–29 C (60–85 F)ComputerHumidity3.5 x 18.3 x 17.9 cm (1.4 x 7.2 x 7.1 in)10% to 90% relative non-condensingRecommended workspaceAir Filtering(W x D x H)180 x 91 x 132 cm(71 x 36 x 52 in)No excessive dust and smokeHeat dissipationLightingOptics and detectors shielded from room.24” LCDRegulatoryStatusFor Research Use Only. Not for use indiagnostic or therapeutic procedures.Cytek Biosciences, Inc.46107 Landing Parkway, Fremont, CA 94538(877) 92-CYTEKsales@cytekbio.com15
Cytek DxP AthenaStart accelerating your research.If you would like more information,email us at: sales@cytekbio.comor call 1-877-92CYTEK
DxP Athena Laser Filter Common Dyes (not exclusive) Blue Laser 45mW FSC 488/10 SSC 488/10 Channel 1 530/30 FITC, Alexa Fluor 488 Channel 2 575/30 PE Channel 3 615/25 PE-Texas Red , PE/Dazzle 594 Channel 4 695/40 PerCP-Cy5.5 Channel 5 780/60 PE-Cy7 Red Laser 80mW Channel 1 661/16 APC Channel 2 710/50 Alexa Fluor 700, APC-Cy5.5 Channel .
