WIXLIB

RADICARE -GOLDYour Personal Faraday Shield1 / RADICARE -GOLD

2 / RADICARE -GOLD

A problem has been detected and RAHN has developed RADICARE -GOLD toprevent damage to the skin.If this is the first time you've become aware of this problem, pleasecarefully take note of the following presentation. If the problem appearsagain, please consider the following fact:At this moment, approximately 900 000 000 000 photons of HEV light arehitting one cm2 of your skin. Your eyes are protected by the carotenoidlutein.Make sure your skin is protected as well. HEV light will increase the ROSlevel of your skin cells.Check with your cosmetic actives vendor for any new radiation protectionactives. Formulate your cosmetics to put your customers in Safe Mode forany radiation threats with RADICARE -GOLD.Check for advanced skin anti-ageing options from RAHN AGTechnical informations:*** STOP:0x0041443154200 -GOLD3 / RADICARECONTINUE(Y/N)(0xRAHNAGSWITZERLAND, WWW.RAHN-GROUP.COM)

Modern lifestyle – lost in connection?0 Hz10 Hz900 MHzLow frequencyfieldsUnplug and relax withRADICARE -GOLDRadio frequencies300 GHzVisible lightDid you know that all kinds ofradiation can create a massiveROS load on skin cells?InfraredUltraviolet4 / RADICARE -GOLD

RADICARE -GOLD in a nutshellYour Personal Faraday ShieldCtrl CCtrl VCtrl. Copy-paste concept: Carotenoids fromthe fresh water algae Tetradesmus obliquus Refills carotenoid depot in the skin barrier Protects against radiation fromvarious sources such as WiFiDelete Reduces ROS production and lipid peroxidationHome Double-action-protection:shields skin barrier and cellsShift Increases the vitality of keratinocytesBreak Decelerates skin ageing for ayouthful appearance5 / RADICARE -GOLD

The skin's own Faraday shieldProtection by carotenoids3. Deposit of carotenoids4. Penetration into the skin barrier2. Transfer of carotenoids via sweat glandsto the skin surface1. Ingestion of carotenoids via nutrition6 / RADICARE -GOLD

Radiation of natural and human origin7 / RADICARE -GOLD

Extrinsic and intrinsic ROS generationcauses cell damage and skin ageing8 / RADICARE -GOLD

The respiratory chainIntrinsic ROS generation by leak electrons9 / RADICARE -GOLD

Tetradesmus obliquusCarotenoids as natural Faraday shield Also known as Acutodesmus or Scenedesmusobliquus One of the most studied fresh water algae Sustainable cultivation in photo bioreactors Forms a natural carotenoid protective shieldwhen exposed to light "Copy alga – paste human" Crambe abyssinica seed oil is used as carrieroil Rich in unsaturated fatty acids (92 %), withthe omega-9 erucic acid (C22:1) Its natural tocopherol content protectsvaluable Tetradesmus obliquus carotenoidsfrom oxidation10 / RADICARE -GOLD

Active compounds from Tetradesmus obliquusβ-carotene and lutein protect lipid membranes against oxidation11 / RADICARE -GOLD

Active compounds in RADICARE -GOLDHPLC fingerprint of carotenoids12 / RADICARE -GOLD

Active compounds in RADICARE -GOLDStability of carotenoidsMethod:Storage test at different conditionsand HPLC analysisImplementation:Determination of total carotenoidsby HPLCResult:Extraordinary stability at roomtemperature. Storage at 20 C, 90 %of the carotenoids are still present.At 40 C, less than 17 % ofthe carotenoids are degraded.13 / RADICARE -GOLD

Cosmetic importanceto protect the skin barrierDeleteOur modern lifestyle constantly increases thestress level of the skin, thus it loses its vitality,elasticity and moistureShiftElevated ROS lead to skin barrier dysfunctionand skin agingInsertThe skin needs double protection – a shield forbarrier and cellsPrintScreenThe skin barrier acts like an antioxidativeprotection shield against intrinsic and extrinsicROS stress factorsInsertStrengthening the skin's own defencemechanism creates a balanced ROS productionCtrlA balanced ROS level is important for a vital skin14 / RADICARE -GOLD

The mode of actionDouble protection for barrier and cells15 / RADICARE -GOLD

RADICARE -GOLDYour Personal Faraday ShieldScientifically confirmed effects: Absorbs high energy visible light (in-vitro study) Activates the cells' oxidative stress defense system(in-vitro study) Relieves cells from oxidative stress (in-vitro study) Reduces blue light-induced oxidative stress (in-vitro study) Reduces WiFi-induced oxidative stress (in-vitro study) Protects cells against lipid peroxidation (in-vitro study) Vitalises the epidermis (in-vitro study) Attenuates the skin's carotenoid loss in the summer season(in-vivo study) Reduces lipid peroxidation and strengthensthe skin barrier (in-vivo study) Increases skin hydration and deceleratesskin ageing (in-vivo study)16 / RADICARE -GOLD

RADICARE -GOLD absorbs high energy visiblelight (in-vitro study)Test designUV/VIS spectroscopy and light transmissionTest itemsProducts in 1cm cuvetteTest formulationsRADICARE -GOLD 50 % in crambe oilBenchmark UVA/UVB sun screen SPF30Primary endpointUV/VIS spectrum: SpectrophotometerSecondary endpointTransmitted light appearance: Photography17 / RADICARE -GOLD

RADICARE -GOLD absorbs high energy visiblelight (in-vitro study)Blue 470 nmUV 395 nm18 / RADICARE -GOLD

RADICARE -GOLD activates the cell's oxidativestress defence system (in-vitro study)Test designIn-vitro cell culture luciferase reporter gene assayTest subjects3T3-L1 adipocytesTest formulationsMedium without RADICARE -GOLD (untreated control)Medium with 0.1 % or 1 % RADICARE -GOLDApplication period6 hoursPrimary endpointPPARα and PPARγ activity: Luciferase assay19 / RADICARE -GOLD

RADICARE -GOLD activates PPARα and PPARγ(in-vitro study)20 / RADICARE -GOLD

RADICARE -GOLD relieves cells fromoxidative stress (in-vitro study)Test designIn-vitro cell culture assayTest subjectsHaCaT-cells (human keratinocytes)Test formulationsMedium without supplements (untreated control)Medium with 0.5 % DMSO with or without 500 μM hydrogen peroxide (vehicle control)Medium with 0.5 % DMSO and 0.005 % T. obliquus carotenoids with or without500 μM hydrogen peroxideApplication period20 minutesPrimary endpointIntracellular ROS content: DCFDA Kit21 / RADICARE -GOLD

RADICARE -GOLD relieves cells fromoxidative stress (in-vitro study)22 / RADICARE -GOLD

RADICARE -GOLD reduces blue light-inducedoxidative stress (in-vitro study)Test designIn-vitro cell culture assayTest subjectsHaCaT-cells (human keratinocytes)Test formulationsMedium without supplements (control)Medium with 0.005 % T. obliquus carotenoidsBlue light irradiation 0 or 13 J/cm2Application period2 hoursPrimary endpointIntracellular ROS content: DFFH-DA assay, flow cyrometry23 / RADICARE -GOLD

RADICARE -GOLD reduces blue light-inducedoxidative stress (in-vitro study)24 / RADICARE -GOLD

RADICARE -GOLD reduces WiFi-inducedoxidative stress (in-vitro study)Test designIn-vitro cell culture assayTest subjectsHaCaT-cells (human keratinocytes)Test formulationsMedium with 0.5 % DMSO (vehicle control)Medium with 0.5 % DMSO and 0.005 % T. obliquus carotenoidsWiFi irradiation 0, 0.5 and 50 mWApplication period5 hoursPrimary endpointIntracellular ROS content: DCFDA Kit25 / RADICARE -GOLD

RADICARE -GOLD reduces WiFi-inducedoxidative stress (in-vitro study)WiFi emitter systemwith Faraday's cage26 / RADICARE -GOLD

RADICARE -GOLD protects cells againstlipid peroxidation (in-vitro study)Test designIn-vitro cell culture assayTest subjectsPrimary human dermal fibroblastsTest formulationsMedium without supplements (untreated control)Medium with 0.5 or 1 % DMSO (vehicle control)Medium with 0.5 or 1 % DMSO and 0.25 % or 0.8 % tocopherolMedium with 0.5 or 1 % DMSO and 0.005 % or 0.01 % T. obliquus carotenoidsWith or without 100 μM cumene hydroperoxideApplication period30 minutesPrimary endpointLipid peroxidation: Image-iT Lipid Peroxidation Kit, epifluorescence imaging27 / RADICARE -GOLD

RADICARE -GOLD protects unstressed cellsagainst lipid peroxidation (in-vitro study)28 / RADICARE -GOLD

RADICARE -GOLD protects stressed cellsagainst lipid peroxidation (in-vitro study)29 / RADICARE -GOLD

RADICARE -GOLD protects cells againstlipid peroxidation (in-vitro study)30 / RADICARE -GOLD

RADICARE -GOLD vitalises the epidermis(in-vitro study)Test designIn-vitro cell culture assayTest subjectsHaCaT-cells (human keratinocytes)Test formulationsMedium without RADICARE -GOLD (untreated control)Medium with 0.1 % RADICARE -GOLDMedium with 10 % FBS (proliferation control)Application period48 hoursPrimary endpointKeratinocytes proliferation: Scratch test, IncuCyte HD imaging31 / RADICARE -GOLD

RADICARE -GOLD promotes lateral keratinocyteproliferation (in-vitro study)32 / RADICARE -GOLD

RADICARE -GOLDYour Personal Faraday Shield- 37 % Lipid peroxidation 68 % Skin barrier 48 % Elasticity- 42 % Less carotenoid depletion 49 % Hydration33 / RADICARE -GOLD- 58 % Roughness

RADICARE -GOLD attenuates the skin'scarotenoid loss in summer (in-vivo study)Test designDouble-blind, placebo controlled, randomisedTest subjects15 female and 9 male subjects with healthy, Caucasian skin, 21 - 56 years (average 40.6)Test formulationsEmulsion containing 0 % RADICARE -GOLD (placebo)Emulsion containing 5 % RADICARE -GOLDApplication areaInner side of forearmsApplication period61 – 64 daysApplication frequencyTwice dailyPrimary endpointsCarotenoid content in the skin:Multiple spatially resolved reflection spectroscopy (MSRRS), Biozoom34 / RADICARE -GOLD

RADICARE -GOLD supports the skin'santioxidative defence level (in-vivo study)35 / RADICARE -GOLD

RADICARE -GOLD reduces lipid peroxidationand strengthens the skin barrier (in-vivo study)Test designDouble-blind, placebo controlled, randomisedTest subjects20 female subjects with healthy, Caucasian skin, 20 - 46 years (average 30.6)Test formulationsEmulsion containing 0 % RADICARE -GOLD (placebo)Emulsion containing 3 % RADICARE -GOLDApplication areaHemifaceApplication period28 daysApplication frequencyTwice dailyPrimary endpointsLipid peroxidation: MDA assay KitDesquamation: Tapestripping (Corneofix F20)36 / RADICARE -GOLD

RADICARE -GOLD reduces lipid peroxidation(in-vivo study)37 / RADICARE -GOLD

RADICARE -GOLD improves the skin barrier(in-vivo study)38 / RADICARE -GOLD

RADICARE -GOLD increases skin hydrationand decelerates skin ageing (in-vivo study)Test designDouble-blind, placebo controlledTest subjects30 female subjects with healthy, Caucasian skin; 35 - 64 years (average 48.8)Test formulationsEmulsion containing 0 % RADICARE -GOLD (placebo)Emulsion containing 3 % RADICARE -GOLDApplication areaInner side of forearmsApplication period28 daysApplication frequencyTwice dailyPrimary endpointsHydration: Corneometer MPA 5Elasticity: Cutometer MPA 580Roughness: Primos 5.739 / RADICARE -GOLD

RADICARE -GOLD improves skin hydration(in-vivo study)40 / RADICARE -GOLD

RADICARE -GOLD increases skin elasticity(in-vivo study)41 / RADICARE -GOLD

RADICARE -GOLD reduces skin roughness(in-vivo study)42 / RADICARE -GOLD

MarketingClaims and benefitsPageUpCtrlEnterCtrl CCtrl VPageDownCtrlStart the day with an dose of extra protection againstHEV-radiation.Protect your skin from free radicals from blue lightor WiFi radiation.Enjoy the modern lifestyle by refillingthe skin barrier with carotenoids.Copy - Paste the protection concept fromthe algae to your skin.Slow down skin aging for a radiant andyouthful appearanceIdeal all-round protection supplement insun products against different types of radiation.Step under your personal Faraday shield, or .43 / RADICARE -GOLD

Should your skin age faster than you?44 / RADICARE -GOLD

Should your skin age faster than you?45 / RADICARE -GOLD